EC - Cholesterol oxidase

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IntEnz Enzyme Nomenclature


Accepted name:
cholesterol oxidase
Other names:
3β-hydroxysteroid:oxygen oxidoreductase
3β-hydroxy steroid oxidoreductase
cholesterol-O2 oxidoreductase
Systematic name:
cholesterol:oxygen oxidoreductase




Contains flavin adenine dinucleotide (FAD). Cholesterol oxidases are secreted bacterial bifunctional enzymes that catalyse the first two steps in the degradation of cholesterol. The enzyme catalyses the oxidation of the 3β-hydroxyl group to a keto group, and the isomerization of the double bond in the oxidized steroid ring system from the Δ5 position to the Δ6 position (cf. EC, steroid Δ-isomerase).

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00543
Structural data: CSA , EC2PDB
Gene Ontology: GO:0016995
CAS Registry Number: 9028-76-6


  1. Richmond, W.
    Preparation and properties of a cholesterol oxidase from Nocardia sp. and its application to the enzymatic assay of total cholesterol in serum.
    Clin. Chem. 19 : 1350-1356 (1973). [PMID: 4757363]
  2. Stadtman, T.C., Cherkes, A. and Anfinsen, C.B.
    Studies on the microbiological degradation of cholesterol.
    J. Biol. Chem. 206 : 511-523 (1954). [PMID: 13143010]
  3. MacLachlan, J., Wotherspoon, A. T., Ansell, R. O., Brooks, C. J.
    Cholesterol oxidase: sources, physical properties and analytical applications.
    J. Steroid Biochem. Mol. Biol. 72 : 169-195 (2000). [PMID: 10822008]
  4. Vrielink, A.
    Cholesterol oxidase: structure and function.
    Subcell. Biochem. 51 : 137-158 (2010). [PMID: 20213543]

[EC created 1961, modified 1982, modified 2012]