EC - Thiamine oxidase

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IntEnz Enzyme Nomenclature


Accepted name:
thiamine oxidase
Other names:
thiamin dehydrogenase
thiamin:oxygen 5-oxidoreductase
thiamine dehydrogenase
Systematic name:
thiamine:oxygen 5-oxidoreductase




A flavoprotein (FAD). The product differs from thiamine in replacement of -CH2.CH2.OH by -CH2.COOH; the two-step oxidation proceeds without the release of the intermediate aldehyde from the enzyme.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0050423
CAS Registry Number: 96779-44-1


  1. Edmondson, D.E., Kenney, W.C. and Singer, T.P.
    Structural elucidation and properties of 8α-(N1-histidyl)riboflavin: the flavin component of thiamine dehydrogenase and β-cyclopiazonate oxidocyclase.
    Biochemistry 15: 2937-2945 (1976). [PMID: 8076]
  2. Gomez-Moreno, C. and Edmondson, D.E.
    Evidence for an aldehyde intermediate in the catalytic mechanism of thiamine oxidase.
    Arch. Biochem. Biophys. 239: 46-52 (1985). [PMID: 2988447]
  3. Neal, R.A.
    Bacterial metabolism of thiamine. 3. Metabolism of thiamine to 3-(2'-methyl-4'-amino-5'-pyrimidylmethyl)-4-methyl-thiazole-5-acetic acid (thiamine acetic acid) by a flavoprotein isolated from a soil microorganism.
    J. Biol. Chem. 245: 2599-2604 (1970). [PMID: 4987737]

[EC created 1984]