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EC 1.1.1.86 - Ketol-acid reductoisomerase (NADP⁺)

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IntEnz Enzyme Nomenclature
EC 1.1.1.86

Names

Accepted name:

ketol-acid reductoisomerase (NADP⁺)

Other names:

2-hydroxy-3-keto acid reductoisomerase
α-keto-β-hydroxylacyl reductoisomerase
acetohydroxy acid isomeroreductase
acetohydroxy acid reductoisomerase
acetolactate reductoisomerase
dihydroxyisovalerate (isomerizing) dehydrogenase
dihydroxyisovalerate dehydrogenase (isomerizing)
isomeroreductase
ketol acid reductoisomerase
reductoisomerase
ketol-acid reductoisomerase
(R)-2,3-dihydroxy-3-methylbutanoate:NADP⁺ oxidoreductase (isomerizing)

Systematic name:

(2R)-2,3-dihydroxy-3-methylbutanoate:NADP⁺ oxidoreductase (isomerizing)

Reactions

22068 [IUBMB]

(2R)-2,3-dihydroxy-3-methylbutanoate

(2R)-2,3-dihydroxy-3-methylbutanoate

Name origin: UniProt - CHECKED (C)

CHEBI:49072

Formula: C5H9O4
Charge: -1

ChEBI compound status: CHECKED (C)

+

NADP⁺

NADP(+)

Name origin: UniProt - CHECKED (C)

CHEBI:58349

Formula: C21H25N7O17P3
Charge: -3

ChEBI compound status: CHECKED (C)

=

(2S)-2-acetolactate

(2S)-2-acetolactate

Name origin: UniProt - CHECKED (C)

CHEBI:58476

Formula: C5H7O4
Charge: -1

ChEBI compound status: CHECKED (C)

+

H⁺

H(+)

Name origin: UniProt - CHECKED (C)

CHEBI:15378

Formula: H
Charge: 1

ChEBI compound status: CHECKED (C)

+

NADPH

NADPH

Name origin: UniProt - CHECKED (C)

CHEBI:57783

Formula: C21H26N7O17P3
Charge: -4

ChEBI compound status: CHECKED (C)
13493 [IUBMB]

(2R,3R)-2,3-dihydroxy-3-methylpentanoate

(2R,3R)-2,3-dihydroxy-3-methylpentanoate

Name origin: UniProt - CHECKED (C)

CHEBI:49258

Formula: C6H11O4
Charge: -1

ChEBI compound status: CHECKED (C)

+

NADP⁺

NADP(+)

Name origin: UniProt - CHECKED (C)

CHEBI:58349

Formula: C21H25N7O17P3
Charge: -3

ChEBI compound status: CHECKED (C)

=

(S)-2-ethyl-2-hydroxy-3-oxobutanoate

(S)-2-ethyl-2-hydroxy-3-oxobutanoate

Name origin: UniProt - CHECKED (C)

CHEBI:49256

Formula: C6H9O4
Charge: -1

ChEBI compound status: CHECKED (C)

+

H⁺

H(+)

Name origin: UniProt - CHECKED (C)

CHEBI:15378

Formula: H
Charge: 1

ChEBI compound status: CHECKED (C)

+

NADPH

NADPH

Name origin: UniProt - CHECKED (C)

CHEBI:57783

Formula: C21H26N7O17P3
Charge: -4

ChEBI compound status: CHECKED (C)

Comments:

Also catalyses the reduction of 2-ethyl-2-hydroxy-3-oxobutanoate to 2,3-dihydroxy-3-methylpentanoate. The enzyme, found in many bacteria and archaea, is specific for NADPH (cf. EC 1.1.1.382, ketol-acid reductoisomerase (NAD⁺) and EC 1.1.1.383, ketol-acid reductoisomerase [NAD(P)⁺]).

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway

Structural data: CSA , EC2PDB

Gene Ontology: GO:0004455

CAS Registry Number: 9075-02-9

UniProtKB/Swiss-Prot: (642) [show] [UniProt]

References

Arfin, S.M. and Umbarger, H.E.

Purification and properties of the acetohydroxy acid isomeroreductase of Salmonella typhimurium.

J. Biol. Chem. 244: 1118-1127 (1969). [PMID: 4388025]
Hill, R.K., Sawada, S. and Arfin, S.M.

Stereochemistry of valine and isoleucine biosynthesis. IV. Synthesis, configuration, and enzymatic specificity of α-acetolactate and α-aceto-α-hydroxybutyrate.

Bioorg. Chem. 8: 175-189 (1979).
Kiritani, K., Narise, S. and Wagner, R.P.

The reductoisomerase of Neurospora crassa.

J. Biol. Chem. 241: 2047-2051 (1966).
Satyanarayana, T. and Radhakrishnan, A.N.

Biosynthesis of valine and isoleucine in plants. 3. Reductoisomerase of Phaseolus radiatus.

Biochim. Biophys. Acta 110: 380-388 (1965). [PMID: 5866387]
Brinkmann-Chen, S., Cahn, J. K., Arnold, F. H.

Uncovering rare NADH-preferring ketol-acid reductoisomerases.

Metab. Eng. 26C: 17-22 (2014). [PMID: 25172159]

[EC 1.1.1.86 created 1972, modified 1976, modified 1981 (EC 1.1.1.89 created 1972, incorporated 1976), modified 2015]

EC 1 - Oxidoreductases

EC 1.1 - Acting on the CH-OH group of donors

EC 1.1.1 - With NAD⁺ or NADP⁺ as acceptor

EC 1.1.1.86 - Ketol-acid reductoisomerase (NADP⁺)

Names

Reactions

Comments:

Links to other databases

References

EC 1.1.1.86 - Ketol-acid reductoisomerase (NADP+)

Names

Reactions

Comments:

Links to other databases

References

EC 1.1.1.86 - Ketol-acid reductoisomerase (NADP⁺)