IntEnz

EC 1.1.1.85 - 3-isopropylmalate dehydrogenase

IntEnz Enzyme Nomenclature
EC 1.1.1.85

Names

Reaction

• 32271 [IUBMB]
  (2R,3S)-3-isopropylmalate

  (2R,3S)-3-isopropylmalate

  Name origin: UniProt - CHECKED (C)

  CHEBI:35121

  Formula: C7H10O5
  Charge: -2

  ChEBI compound status: CHECKED (C)

  

  +

  NAD⁺

  NAD(+)

  Name origin: UniProt - CHECKED (C)

  CHEBI:57540

  Formula: C21H26N7O14P2
  Charge: -1

  ChEBI compound status: CHECKED (C)

  

  =

  4-methyl-2-oxopentanoate

  4-methyl-2-oxopentanoate

  Name origin: UniProt - CHECKED (C)

  CHEBI:17865

  Formula: C6H9O3
  Charge: -1

  ChEBI compound status: CHECKED (C)

  

  +

  CO₂

  CO2

  Name origin: UniProt - CHECKED (C)

  CHEBI:16526

  Formula: CO2
  Charge: 0

  ChEBI compound status: CHECKED (C)

  

  +

  NADH

  NADH

  Name origin: UniProt - CHECKED (C)

  CHEBI:57945

  Formula: C21H27N7O14P2
  Charge: -2

  ChEBI compound status: CHECKED (C)

  

Comments:

The product decarboxylates spontaneously to yield 4-methyl-2-oxopentanoate.

Links to other databases

References

1. Burns, R.O., Umbarger, H.E. and Gross, S.R.
   The biosynthesis of leucine. III. The conversion of α-hydroxy-β-carboxyisocaproate to α-ketoisocaproate.
   Biochemistry 2: 1053 (1963). [PMID: 14087358]
2. Parsons, S.J. and Burns, R.O.
   Purification and properties of β-isopropylmalate dehydrogenase.
   J. Biol. Chem. 244: 996-1003 (1969). [PMID: 4889950]
3. Németh, A., Svingor, Á., Pócsik, M., Dobó, J., Magyar, C., Szilagyi, A., Gál, P., Závodszky, P.
   Mirror image mutations reveal the significance of an intersubunit ion cluster in the stability of 3-isopropylmalate dehydrogenase.
   FEBS Lett. 468: 48-52 (2000). [PMID: 10683439]
4. Calvo, J.M., Stevens, C.M., Kalyanpur, M.G. and Umbarger, H.E.
   The absolute configuration of alpha-hydroxy-beta-carboxyisocaproic acid (3-isopropylmalic acid), an intermediate in leucine biosynthesis.
   Biochemistry 3: 2024-2027 (1964). [PMID: 14269331]

[EC 1.1.1.85 created 1972, modified 1976]