EC - 3α(or 20β)-hydroxysteroid dehydrogenase

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IntEnz Enzyme Nomenclature


Accepted name:
3α(or 20β)-hydroxysteroid dehydrogenase
Other names:
(R)-20-hydroxysteroid dehydrogenase
20β-hydroxysteroid dehydrogenase
Δ4-3-ketosteroid hydrogenase
NADH-20β-hydroxysteroid dehydrogenase
cortisone reductase
dehydrogenase, 20β-hydroxy steroid
Systematic name:
3α(or 20β)-hydroxysteroid:NAD+ oxidoreductase



The 3α-hydroxy group or 20β-hydroxy group of pregnane and androstane steroids can act as donor.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00060
Structural data: CSA , EC2PDB
Gene Ontology: GO:0047044
CAS Registry Number: 9028-42-6


  1. Edwards, C.A.F. and Orr, J.C.
    Comparison of the 3α-and 20β-hydroxysteroid dehydrogenase activities of the cortisone reductase of Streptomyces hydrogenans.
    Biochemistry 17: 4370-4376 (1978). [PMID: 718844]
  2. Hübener, H.J. and Sahrholz, F.G.
    20β-hydroxy-steroid-dehydrogenase. II. Darstellung und Kristallisation.
    Biochem. Z. 333: 95-105 (1960).
  3. Hübener, H.J., Sahrholz, F.G., Schmidt-Thomé, J., Nesemann, G. and Junk, R.
    20β-Hydroxy-Steroid-Dehydrogenase, ein neues kristallines Enzym.
    Biochim. Biophys. Acta 35: 270-272 (1959).
  4. Lynn, W.S. and Brown, R.H.
    The conversion of progesterone to androgens by testes.
    J. Biol. Chem. 232: 1015-1030 (1958).
  5. Strickler, R.C., Covey, D.F. and Tobias, B.
    Study of 3α, 20 β-hydroxysteroid dehydrogenase with an enzyme-generated affinity alkylator: dual enzyme activity at a single active site.
    Biochemistry 19: 4950-4954 (1980). [PMID: 6936053]
  6. Sweet, F. and Samant, B.S.
    Bifunctional enzyme activity at the same active site: study of 3α and 20β activity by affinity alkylation of 3α, 20β-hydroxysteroid dehydrogenase with 17-(bromoacetoxy)steroids.
    Biochemistry 19: 978-986 (1980). [PMID: 6928375]

[EC created 1961, modified 1986]