EC 188.8.131.523 - Ephedrine dehydrogenase
IntEnz Enzyme Nomenclature
- (1R,2S)-()-ephedrine + NAD+ = (S)-2-methylamino-1-phenylpropan-1-one + NADH + H+
The enzyme, characterized from the bacterium Arthrobacter sp. TS-15, acts on a broad range of different aryl-alkyl ketones, such as haloketones, ketoamines, diketones, and ketoesters. It accepts various types of aryl groups including phenyl-, pyridyl-, thienyl-, and furyl-rings, but the presence of an aromatic ring is essential for the activity. In addition, the presence of a functional group on the alkyl chain, such as an amine, a halogen, or a ketone, is also crucial. The enzyme exhibits a strict Prelog enantioselectivity. When acting on diketones, it catalyses the reduction of only the keto group closest to the ring, with no further reduction to the diol. cf. EC 184.108.40.2062.
Links to other databases
Two enantiocomplementary ephedrine dehydrogenases from Arthrobacter sp. TS-15 with broad substrate specificity.ACS Catal. 6202-6211 (2019).
[EC 220.127.116.113 created 2020]