EC 1 - Oxidoreductases
EC 1.1 - Acting on the CH-OH group of donors
EC 1.1.1 - With NAD+ or NADP+ as acceptor
EC 1.1.1.42 - Isocitrate dehydrogenase (NADP+)
IntEnz view
ENZYME view
IntEnz Enzyme Nomenclature
EC 1.1.1.42
Names
Accepted name:
isocitrate dehydrogenase (NADP+)
Other
names:
NADP isocitric dehydrogenase
NADP-dependent isocitrate dehydrogenase
NADP-dependent isocitric dehydrogenase
NADP-linked isocitrate dehydrogenase
NADP-specific isocitrate dehydrogenase
NADP+-linked isocitrate dehydrogenase
isocitrate (NADP) dehydrogenase
isocitrate (nicotinamide adenine dinucleotide phosphate) dehydrogenase
isocitrate dehydrogenase (NADP)
isocitrate dehydrogenase (NADP-dependent)
oxalosuccinate decarboxylase
oxalsuccinic decarboxylase
IDH [ambiguous]
triphosphopyridine nucleotide-linked isocitrate dehydrogenase-oxalosuccinate carboxylase
dual-cofactor-specific isocitrate dehydrogenase
NADP+-ICDH
NADP+-IDH
IDP
IDP1
IDP2
IDP3
NADP-dependent isocitrate dehydrogenase
NADP-dependent isocitric dehydrogenase
NADP-linked isocitrate dehydrogenase
NADP-specific isocitrate dehydrogenase
NADP+-linked isocitrate dehydrogenase
isocitrate (NADP) dehydrogenase
isocitrate (nicotinamide adenine dinucleotide phosphate) dehydrogenase
isocitrate dehydrogenase (NADP)
isocitrate dehydrogenase (NADP-dependent)
oxalosuccinate decarboxylase
oxalsuccinic decarboxylase
IDH [ambiguous]
triphosphopyridine nucleotide-linked isocitrate dehydrogenase-oxalosuccinate carboxylase
dual-cofactor-specific isocitrate dehydrogenase
NADP+-ICDH
NADP+-IDH
IDP
IDP1
IDP2
IDP3
Systematic name:
isocitrate:NADP+ oxidoreductase (decarboxylating)
Reactions
- (1) isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH + H+
- (1a) isocitrate + NADP+ = 2-oxalosuccinate + NADPH + H+
- (1b) 2-oxalosuccinate = 2-oxoglutarate + CO2
Cofactor
Comments:
Requires Mn2+ or Mg2+ for activity. Unlike EC 1.1.1.41, isocitrate dehydrogenase (NAD+), oxalosuccinate can be used as a substrate. In eukaryotes, isocitrate dehydrogenase exists in two forms: an NAD+-linked enzyme found only in mitochondria and displaying allosteric properties, and a non-allosteric, NADP+-linked enzyme that is found in both mitochondria and cytoplasm [6]. The enzyme from some species can also use NAD+ but much more slowly [6,7].
Links to other databases
Enzymes and pathways:
NC-IUBMB
,
BRENDA
,
DIAGRAM
,
ExplorEnz
,
ENZYME@ExPASy
,
KEGG
,
MetaCyc
,
NIST 74
,
UniPathway
Protein domains and families:
PROSITE:PDOC00389
Gene Ontology:
GO:0004450
CAS Registry Number:
9028-48-2
References
-
Partial purification and characterization of the isocitric dehydrogenase from Trypanosoma cruzi.Biochim. Biophys. Acta 21 : 117-126 (1956). [PMID: 13363868]
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Purification of the isocitrate enzyme (triphosphopyridine nucleotide-linked isocitrate dehydrogenase-oxalosuccinate carboxylase).Biochem. J. 63 : 548-552 (1956). [PMID: 13355848]
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Isocitrate dehydrogenases.In: Boyer, P.D., Lardy, H. and Myrbäck, K. (Eds.) The Enzymes , 2nd ed. vol. 7 , Academic Press , New York , 1963 , 105-126
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The preparation of isocitrate dehydrogenase from mammalian heart.J. Biol. Chem. 226 : 965-975 (1957). [PMID: 13438885]
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A suggested new nomenclature for the isomers of isocitric acid.J. Biol. Chem. 237 : 1739-1741 (1962). [PMID: 13925783]
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Isocitrate dehydrogenases from Haloferax volcanii and Sulfolobus solfataricus: enzyme purification, characterisation and N-terminal sequence.FEMS Microbiol. Lett. 134 : 85-90 (1996). [PMID: 8593959]
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Biochemical and phylogenetic characterization of isocitrate dehydrogenase from a hyperthermophilic archaeon, Archaeoglobus fulgidus.Arch. Microbiol. 168 : 412-420 (1998). [PMID: 9325430]
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Cytosolic NADP+-dependent isocitrate dehydrogenase plays a key role in lipid metabolism.J. Biol. Chem. 279 : 39968-39974 (2004). [PMID: 15254034]
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Crystal structure of porcine mitochondrial NADP+-dependent isocitrate dehydrogenase complexed with Mn2+ and isocitrate. Insights into the enzyme mechanism.J. Biol. Chem. 277 : 43454-43462 (2002). [PMID: 12207025]
[EC 1.1.1.42 created 1961, modified 2005]