EC - Isocitrate dehydrogenase (NAD+)

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IntEnz Enzyme Nomenclature


Accepted name:
isocitrate dehydrogenase (NAD+)
Other names:
β-ketoglutaric-isocitric carboxylase
NAD dependent isocitrate dehydrogenase
NAD isocitrate dehydrogenase
NAD isocitric dehydrogenase
NAD-linked isocitrate dehydrogenase
NAD-specific isocitrate dehydrogenase
isocitric acid dehydrogenase
isocitric dehydrogenase
isocitrate dehydrogenase (NAD)
IDH [ambiguous]
nicotinamide adenine dinucleotide isocitrate dehydrogenase
Systematic name:
isocitrate:NAD+ oxidoreductase (decarboxylating)




Requires Mn2+ or Mg2+ for activity. Unlike EC, isocitrate dehydrogenase (NADP+), oxalosuccinate cannot be used as a substrate. In eukaryotes, isocitrate dehydrogenase exists in two forms: an NAD+-linked enzyme found only in mitochondria and displaying allosteric properties, and a non-allosteric, NADP+-linked enzyme that is found in both mitochondria and cytoplasm [7]. The enzyme from some species can also use NADP+ but much more slowly [9].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00389
Structural data: CSA , EC2PDB
Gene Ontology: GO:0004449
CAS Registry Number: 9001-58-5
UniProtKB/Swiss-Prot: (26) [show] [UniProt]


  1. Hathaway, J.A. and Atkinson, D.E.
    The effect of adenylic acid on yeast nicotinamide adenine dinucleotide isocitrate dehydrogenase, a possible metabolic control mechanism.
    J. Biol. Chem. 238: 2875-2881 (1963). [PMID: 14063317]
  2. Kornberg, A. and Pricer, W.E.
    Di- and triphosphopyridine nucleotide isocitric dehydrogenase in yeast.
    J. Biol. Chem. 189: 123-136 (1951). [PMID: 14832224]
  3. Plaut, G.W.E.
    Isocitrate dehydrogenases.
    In: Boyer, P.D., Lardy, H. and Myrbäck, K. (Eds.) The Enzymes, 2nd ed. vol. 7, Academic Press, New York, 1963, 105-126
  4. Plaut, G.W.E. and Sung, S.-C.
    Diphosphopyridine nucleotide isocitric dehydrogenase from animal tissues.
    J. Biol. Chem. 207: 305-314 (1954). [PMID: 13152105]
  5. Ramakrishnan, C.V. and Martin, S.M.
    Isocitric dehydrogenase in Aspergillus niger.
    Arch. Biochem. Biophys. 55: 403-407 (1955).
  6. Vickery, H.B.
    A suggested new nomenclature for the isomers of isocitric acid.
    J. Biol. Chem. 237: 1739-1741 (1962). [PMID: 13925783]
  7. Camacho, M.L., Brown, R.A., Bonete, M.-J., Danson, M.J. and Hough, D.W.
    Isocitrate dehydrogenases from Haloferax volcanii and Sulfolobus solfataricus: enzyme purification, characterisation and N-terminal sequence.
    FEMS Microbiol. Lett. 134: 85-90 (1996). [PMID: 8593959]
  8. Kim, Y.-O., Koh, H.-J., Kim, S.-H., Jo, S.-H., Huh, J.-W., Jeong, K.-S., Lee, I.J., Song, B.J. and Huh, T.-L.
    Identification and functional characterization of a novel, tissue-specific NAD+-dependent isocitrate dehydrogenase β subunit isoform.
    J. Biol. Chem. 274: 36866-36875 (2000). [PMID: 10601238]
  9. Inoue, H., Tamura, T., Ehara, N., Nishito, A., Nakayama, Y., Maekawa, M., Imada, K., Tanaka, H. and Inagaki, K.
    Biochemical and molecular characterization of the NAD+-dependent isocitrate dehydrogenase from the chemolithotroph Acidithiobacillus thiooxidans.
    FEMS Microbiol. Lett. 214: 127-132 (2002). [PMID: 12204383]

[EC created 1961, modified 2005]