EC - 3α-hydroxysteroid 3-dehydrogenase

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IntEnz Enzyme Nomenclature


Accepted name:
3α-hydroxysteroid 3-dehydrogenase
Other names:
3α-hydroxysteroid dehydrogenase
AKR1C4 (gene name)
AKR1C2 (gene name)
hsdA (gene name)
Systematic name:
3α-hydroxysteroid:NAD(P)+ 3-oxidoreductase



The enzyme acts on multiple 3α-hydroxysteroids, such as androsterone and 5 α-dihydrotestosterone. The mammalian enzymes are involved in inactivation of steroid hormones, while the bacterial enzymes are involved in steroid degradation. This entry stands for enzymes whose stereo-specificity with respect to NAD+ or NADP+ is not known. [cf. EC, 3α-hydroxysteroid 3-dehydrogenase (Si-specific) and EC, 3α-hydroxysteroid 3-dehydrogenase (Re-specific)].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB


  1. Deyashiki, Y., Ogasawara, A., Nakayama, T., Nakanishi, M., Miyabe, Y., Sato, K., Hara, A.
    Molecular cloning of two human liver 3 alpha-hydroxysteroid/dihydrodiol dehydrogenase isoenzymes that are identical with chlordecone reductase and bile-acid binder.
    Biochem. J. 299: 545-552 (1994). [PMID: 8172617]
  2. Khanna, M., Qin, K. N., Wang, R. W., Cheng, K. C.
    Substrate specificity, gene structure, and tissue-specific distribution of multiple human 3 alpha-hydroxysteroid dehydrogenases.
    J. Biol. Chem. 270: 20162-20168 (1995). [PMID: 7650035]
  3. Oppermann, U. C., Maser, E.
    Characterization of a 3 alpha-hydroxysteroid dehydrogenase/carbonyl reductase from the gram-negative bacterium Comamonas testosteroni.
    Eur. J. Biochem. 241: 744-749 (1996). [PMID: 8944761]
  4. Mobus, E., Maser, E.
    Molecular cloning, overexpression, and characterization of steroid-inducible 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni. A novel member of the short-chain dehydrogenase/reductase superfamily.
    J. Biol. Chem. 273: 30888-30896 (1998). [PMID: 9812981]
  5. Nahoum, V., Gangloff, A., Legrand, P., Zhu, D. W., Cantin, L., Zhorov, B. S., Luu-The, V., Labrie, F., Breton, R., Lin, S. X.
    Structure of the human 3alpha-hydroxysteroid dehydrogenase type 3 in complex with testosterone and NADP at 1.25-A resolution.
    J. Biol. Chem. 276: 42091-42098 (2001). [PMID: 11514561]

[EC created 2013]