EC 1.1.1.283 - Methylglyoxal reductase (NADPH)

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IntEnz Enzyme Nomenclature
EC 1.1.1.283

Names

Accepted name:
methylglyoxal reductase (NADPH)
Other names:
lactaldehyde dehydrogenase (NADP+)
GRE2 (gene name)
methylglyoxal reductase (NADPH-dependent)
lactaldehyde:NADP+ oxidoreductase
Systematic name:
(S)-lactaldehyde:NADP+ oxidoreductase

Reaction

Comments:

The enzyme from the yeast Saccharomyces cerevisiae catalyses the reduction of a keto group in a number of compounds, forming enantiopure products. Among the substrates are methylglyoxal (which is reduced to (S)-lactaldehyde) [1,2], 3-methylbutanal [3], hexane-2,5-dione [4] and 3-chloro-1-phenylpropan-1-one [5]. The enzyme differs from EC 1.1.1.78, methylglyoxal reductase (NADH), which is found in mammals, by its coenzyme requirement, reaction direction, and enantiomeric preference.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0043892
UniProtKB/Swiss-Prot:

References

  1. Murata, K., Fukuda, Y., Simosaka, M., Watanabe, K., Saikusa, T. and Kimura, A.
    Metabolism of 2-oxoaldehyde in yeasts. Purification and characterization of NADPH-dependent methylglyoxal-reducing enzyme from Saccharomyces cerevisiae.
    Eur. J. Biochem. 151: 631-636 (1985). [PMID: 3896793]
  2. Chen, C.N., Porubleva, L., Shearer, G., Svrakic, M., Holden, L.G., Dover, J.L., Johnston, M., Chitnis, P.R. and Kohl, D.H.
    Associating protein activities with their genes: rapid identification of a gene encoding a methylglyoxal reductase in the yeast Saccharomyces cerevisiae.
    Yeast 20: 545-554 (2003). [PMID: 12722185]
  3. Hauser, M., Horn, P., Tournu, H., Hauser, N. C., Hoheisel, J. D., Brown, A. J., Dickinson, J. R.
    A transcriptome analysis of isoamyl alcohol-induced filamentation in yeast reveals a novel role for Gre2p as isovaleraldehyde reductase.
    FEMS Yeast Res. 7: 84-92 (2007). [PMID: 16999827]
  4. Muller, M., Katzberg, M., Bertau, M., Hummel, W.
    Highly efficient and stereoselective biosynthesis of (2S,5S)-hexanediol with a dehydrogenase from Saccharomyces cerevisiae.
    Org. Biomol. Chem. 8: 1540-1550 (2010). [PMID: 20237665]
  5. Choi, Y. H., Choi, H. J., Kim, D., Uhm, K. N., Kim, H. K.
    Asymmetric synthesis of (S)-3-chloro-1-phenyl-1-propanol using Saccharomyces cerevisiae reductase with high enantioselectivity.
    Appl. Microbiol. Biotechnol. 87: 185-193 (2010). [PMID: 20111861]
  6. Breicha, K., Muller, M., Hummel, W., Niefind, K.
    Crystallization and preliminary crystallographic analysis of Gre2p, an NADP(+)-dependent alcohol dehydrogenase from Saccharomyces cerevisiae.
    Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 66: 838-841 (2010). [PMID: 20606287]

[EC 1.1.1.283 created 2005, modified 2013]