EC - D-2-hydroxyacid dehydrogenase (NADP+)

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IntEnz Enzyme Nomenclature


Accepted name:
D-2-hydroxyacid dehydrogenase (NADP+)
Other name:
ddh (gene name)
Systematic name:
(R)-2-hydroxycarboxylate:NADP+ oxidoreductase



This enzyme, characterized from the halophilic archaeon Haloferax mediterranei and the mold Aspergillus oryzae, catalyses a reversible stereospecific reduction of 2-oxocarboxylic acids into the corresponding D-2-hydroxycarboxylic acids. The enzyme prefers substrates with a main chain of 5 carbons (such as 4-methyl-2-oxopentanoate) to those with a shorter chain, and can use NADH with much lower efficiency. cf. EC, (D)-2-hydroxyacid dehydrogenase (NAD+).

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0050578


  1. Domenech, J., Ferrer, J.
    A new D-2-hydroxyacid dehydrogenase with dual coenzyme-specificity from Haloferax mediterranei, sequence analysis and heterologous overexpression.
    Biochim. Biophys. Acta 1760: 1667-1674 (2006). [PMID: 17049749]
  2. Shimizu, M., Yamamoto, T., Okabe, N., Sakai, K., Koide, E., Miyachi, Y., Kurimoto, M., Mochizuki, M., Yoshino-Yasuda, S., Mitsui, S., Ito, A., Murano, H., Takaya, N. and Kato, M.
    Novel 4-methyl-2-oxopentanoate reductase involved in synthesis of the Japanese sake flavor, ethyl leucate.
    Appl. Microbiol. Biotechnol. (2015). [PMID: 26615399]

[EC created 2002, modified 2013]