EC 1.1.1.203 - Uronate dehydrogenase

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IntEnz Enzyme Nomenclature
EC 1.1.1.203

Names

Accepted name:
uronate dehydrogenase
Other names:
uronate:NAD-oxidoreductase
uronic acid dehydrogenase
Systematic name:
uronate:NAD+ 1-oxidoreductase

Reactions

Cofactor

Comments:

Requires Mg2+. The enzyme, characterized from the bacterium Agrobacterium fabrum, participates in oxidative degradation pathways for galacturonate and glucuronate. The enzyme can only accept the β anomeric form of the substrate [4]. The 1,5 lactone product is rather stable at cytosolic pH and does not hydrolyse spontaneously at a substantial rate. Formerly EC 1.2.1.35.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ERGO , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0050388
CAS Registry Number: 37250-98-9
UniProtKB/Swiss-Prot:

References

  1. Kilgore, W.W. and Starr, M.P.
    Uronate oxidation by phytopathogenic pseudomonads.
    Nature 183: 1412-1413 (1959). [PMID: 13657147]
  2. Boer, H., Maaheimo, H., Koivula, A., Penttila, M., Richard, P.
    Identification in Agrobacterium tumefaciens of the D-galacturonic acid dehydrogenase gene.
    Appl. Microbiol. Biotechnol. 86: 901-909 (2010). [PMID: 19921179]
  3. Andberg, M., Maaheimo, H., Boer, H., Penttila, M., Koivula, A., Richard, P.
    Characterization of a novel Agrobacterium tumefaciens galactarolactone cycloisomerase enzyme for direct conversion of D-galactarolactone to 3-deoxy-2-keto-L-threo-hexarate.
    J. Biol. Chem. 287: 17662-17671 (2012). [PMID: 22493433]
  4. Parkkinen, T., Boer, H., Janis, J., Andberg, M., Penttila, M., Koivula, A., Rouvinen, J.
    Crystal structure of uronate dehydrogenase from Agrobacterium tumefaciens.
    J. Biol. Chem. 286: 27294-27300 (2011). [PMID: 21676870]

[EC 1.1.1.203 created 1972 as EC 1.2.1.35, transferred 1984 to EC 1.1.1.203, modified 2014]