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What is this view?

Binary interactions

In this tab, we display the list of interactions that you have selected using one of our search features. Despite the fact that our data are annotated to accurately reflect the interactions reported in scientific literature, the data is shown in this view as binary interactions. Whenever the data was reported as a co-complex involving more than two molecules, we store it as such in the IntAct database and post-process it so the portal can show it as binary interaction. This post-processing is the Spoke Expansion model (connects bait to all preys):



sourceExp

At any moment you can choose to display the expansion column in this view in order to see which interaction are spoke expanded and which are not.

Description of what has changed

  • We have added more download options to allow users to retrieve their interaction set using more standard formats such as PSI-MI XML and PSIMITAB (version 2.5, 2.6 or 2.7) but also XGMML, RDF and Biopax (level 2 and 3).
  • We have now four different table views : minimal(molecule names and interaction AC), basic (minimal + molecule links, interaction detection method, negative), standard(minimal + molecule species, confidences, publication details, experiment details), expanded (standard + more experiment details) and complete (all mitab 2.7 columns).

Configuring the view to your need"

In the header of the interaction table you will find a button: ‘Change Column Display’ that will show you all the columns/Table views available and allow you to update the current selected set.

Downloading the data into Standard formats"

In the header of the interaction table you will find a drop down list that contains all the formats currently supported when downloading the interaction data. Select one of them and click the export button next to the list. Please note that PSI-MI XML is only available when the interaction set is no bigger than 1000 interactions.

Opening the interaction details"

Clicking on the magnifying glass in the first column of the interaction table will open the details of the corresponding interaction in the Interaction Details tab, giving you access to more details of the manually curated record.

What is this view?

Browsing (Browse Tab)

This tab is meant to give you access to more content based on the currently selected set of interactions. Please note that linking to third party resources will only include up to 200 molecules , if you exceed this number you will see the warning icon (This number has been reduced to 125 molecules for mRNA expression). Now let’s look at the features available to you:

Limiting the scope of the current dataset with the Uniprot Taxonomy ontology

Allows users to browse the Uniprot Taxonomy hierarchy as a tree and select terms in order to narrow down their dataset. Once a term is selected, you are taken back to the interaction tab to review your dataset.

Limiting the scope of the current dataset with the GO ontology

Allows users to browse the GO hierarchy as a tree and select terms in order to narrow down their dataset. Once a term is selected, you are taken back to the interaction tab to review your dataset.

Limiting the scope of the current dataset with the ChEBI ontology

Allows users to browse the ChEBI hierarchy as a tree and select terms in order to narrow down their dataset. Once a term is selected, you are taken back to the interaction tab to review your dataset.

Bulk linking to third party resources by using involved proteins

  • Proteins by Reactome pathway: Sends your proteins to the Reactome SkyPainter that will show you the pathways in which these molecules are know to play a role.
  • Proteins by Chromosomal location: Sends your list of proteins to Ensembl’s Karyotype viewer and overlays the proteins on the chromosomes.
  • Proteins by mRNA expression: Sends your set of proteins to the ArrayExpress Atlas that will show the known gene expression based on experimental studies.
What is this view?

Searching Interactions (Search Tab)

As you can see in this tab we are now trying to give you more targeted choice to do your queries, please note that the examples provided in this tab are live links so you can simply click them to see the resulting interactions sets.

Using the Quick Search

In this search panel you are free to type anything that might relate to interactions, whether it is properties of their interactor (gene name, identifiers, GO term…) or more specific to the interaction like publication, authors, experimental detection method, ...

Some examples:

  • Try the query: imatinib
    This is a drug for which we have curated a number of interactions.
    Once you press the search button you should be taken to the Interaction Tab that lists 130 binary interactions.
    If you want to construct more complex queries we recommend you take a look at the Molecular Interaction Query Language, accessible from the quick search panel.
  • Try the query: species:yeast AND type:"direct interaction"
    This query selects all interactions involving yeast interactors that have been shown to have direct interactions. If you customize the column display of the interaction tab, you will see that not only “direct interaction” have been selected but also children terms in the PSI-MI ontology.

Using the Ontology Search

Open the Rearch Tab. This panel is specialised to give you an easy access to ontology search. So far you can search on 4 ontologies:

  • Gene Ontology
  • InterPro
  • PSI-MI
  • ChEBI

Whenever you start typing a query in this search panel, the system will search as you type and propose a list of matching controlled vocabulary terms. You can then select one of them and select matching interactions.

For example, type: cancer
You will be presented with a few choices, please note that each term is followed by the count of matching interactions in the IntAct database.

Select a term with the mouse or using the keyboard cursor keys and you will be taken to the interaction tab.

Searching the Compound chemical structure

In this panel you will be able to draw all or part of a chemical structure and search for chemical compounds. If you get any matched, you can then see all interactions involving them.

First you have to open up the chemical search panel so that the applet can load, it might take a few seconds. Then you can start drawing your structure, for instance:

Once you have drawn your structure, select Similarity and press Search. You should be presented with a list of matching compound. Now choose one molecule and click the link: IntAct interactions. You will be taken to the interaction tab to review the data.

Complex Expansion

Binary interactions generated by co-complex expansion

Why should you care about complex expansion ?

Some experimental methods such as Tandem Affinity Purification do generate molecular interactions that can involve more than 2 molecules. Despite the fact that IntAct curation team do capture the molecular interaction as they were reported in the corresponding experiment, when you search using the intact web site, the results of your query is always shown as set of binary interactions (i.e. 2 molecules). We would like to draw your attention on the fact that whenever the reported interaction was a co-complex we do apply an expansion algorithm that transform this n-ary interaction into a set of binary interactions. While none of these agorithms is perfect and will very likely generate some false positive interactions, it is useful to present the data in a consistent manner. Bear in mind that we will strive to differentiate in the search results which interactions are a real experimental binary from expanded ones.

Existing expansion algorithm

There are several known algorithm allowing to transform an n-ary interaction into a set of binaries. The illustration below present the two well known expansion model and illustrates why they can be incorrect.



sourceExp

  • Spoke expansion: Links the bait molecule to all prey molecules. If N is the count of molecule in the complex, it generated N-1 binary interactions.
  • Matrix expansion: Links all molecule to all other molecule present in the complex. If N is the count of molecule in the complex, it generated (N*(N-1))/2 binary interactions.

Now the issue (as illustrated at the bottom right of the diagram above) with these two models lies in the fact that the real complex might not be articulated around the experimental bait but instead, this bait might be linked to a smaller complex, hence most binary interaction generated by spoke and matrix expansion result in false positive.



PSICQUIC

How is the number of interactions in other databases obtained?

PSICQUIC is a standard way to access molecular interaction databases across which it repeats the same query. The number of databases providing data may vary, depending on the status of their services and only those that are active are used in this query. By clicking on the number of interactions you will be redirected to the PSICQUIC View, where you can browse the results in those other resources.

The services currently active are:

Check the PSICQUIC site for more information.

IMEx

What is the significance of the IMEx dataset?"

IMEx is a network of databases which have agreed to supply a non-redundant set of data expertly manually annotated to the same consistent detailed standard which, as such, represents a high-quality subset of the data each individually provides. The number of databases providing data may vary, depending on the status of their services and only those that are active are used in this query. By clicking on the number of interactions you will be redirected to the IMEx View, where you can browse the results in those other resources.

The services currently active are:

Check the IMEx site for more information.

What is this view?

Representation of Experimental Features

This section shows the graphical representation of experimental features, where each participant is represented as a white rectangle with a black border and a line for each hundredth amino acid. All available features are attached to their associated participant and their categories are represented in the right side of the legend. The left side of the legend dynamically shows the range statuses occuring in the shown interaction. These are the possible range statuses:

sourceExp

Interacting with the widget

Hover over a feature to see more information in a tooltip.
sourceExp

To display a single interacting region click on it and click again to display all interacting regions.
Displaying all interacting regionsDisplaying one interacting region
sourceExpsourceExp
What is this view?

Dynamic molecular interaction data

This section shows the graphical representation of dynamic molecular interactions. By default it displays all the interactions from one experiment using radio buttons to allow users to highlight interactions in different variable conditions.

Publication

PubMed Id: 21903422

Journal: Immunity (1074-7613)

Year of Publication: 2011

Title: Mapping a dynamic innate immunity protein interaction network regulating type I interferon production.

Author List: Li S., Wang L., Berman M., Kong Y.Y., Dorf M.E.

Cross References:

Database
Identifier
Secondary identifier
Qualifier
-
-

Experiment (47 interactions)

Accession: EBI-6115363

Name: li-2011j-1

Host organism: Homo sapiens transformed primary embryonal kidney cells

Organism: Homo sapiens transformed primary embryonal kidney cells

Accession: EBI-307628

Name: human-293

Description: Homo sapiens transformed primary embryonal kidney cells

Cross References:

Database
Identifier
Secondary identifier
Qualifier
human
-
-

Interaction Detection Method: anti tag coip

Interaction detection method: anti tag coip
Accession: EBI-90

Name: anti tag coip

Description: anti tag coimmunoprecipitation

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0007
-
identity
pubmed
7708014
-
primary-reference


Annotations:
Topic
Text
definition
A specific antibody for the molecule of interest is not available, therefore the bait protein is expressed as a hybrid protein fused to a tag peptide/protein for which efficient and specific antibodies or a specific ligand are available.


Participant Identification Method: sequence tag

Participant identification method: sequence tag
Accession: EBI-81

Name: sequence tag

Description: sequence tag identification

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0102
-
identity
pubmed
10967324
-
primary-reference
pubmed
11752590
-
primary-reference
pubmed
11805837
-
primary-reference


Annotations:
Topic
Text
definition
This approach leads to protein identification by combining mass measurement and short amino acid sequence information obtained by tandem mass spectrometry. This information is then used to automatically find the best match in a sequence database. A mixture of peptides derived from a protease digestion is analysed by nanoelectrospray LC-MS/MS (Liquid Chromatography Tandem Mass Spectrometer or nanoESI MS/MS) mass spectrometry. Electrospray mass spectrometry cannot be applied to dilute samples and is affected by high salt. As a consequence peptides, normally extracted from acrylamide gels by in situ proteolysis, are desalted and concentrated on a microcolumn followed by elution into a capillary used for nanoelectrospray tandem mass spectrometry. A first mass spectrum (Normal mass spectrum or Q1 mass spectrum) gives information about the masses of all the peptides. Peptides observed in the normal mass spectrum are isolated in turn and dissociated into fragments by collision with gas molecules within the mass spectrometer. Some of the fragments obtained from a peptide constitute a nested set, differing by one amino acid, and the mass difference between them allows assignment of a partial sequence. The masses of the fragments define the position of the partial sequence in the peptide. Together with the cleavage specificity of the protease used to cleave the protein, and mass information such sequence tag provides much higher search specificity to match the a database entry. The procedure is repeated with several peptides from the digest, resulting in multiple identifications of the same protein or identification of several proteins from the peptide mixture. Unknown proteins can easily be identified by using the high specificity of the peptide sequence tag for searches in most sequence databases including EST or genome databases.


Cross References:

Database
Identifier
Secondary identifier
Qualifier
-
-

Annotations:

Topic
Text
dorf@hms.harvard.edu
imex curation
Only protein-protein interactions
-

Interaction

Accession: EBI-6115727

Name: mavs-bag6

Description: -

Type: association

Interaction type: association
Accession: EBI-1813191

Name: association

Description: association

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0914
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Interaction between molecules that may participate in formation of one, but possibly more, physical complexes. Often describes a set of molecules that are co-purified in a single pull-down or coimmunoprecipitation but might participate in formation of distinct physical complexes sharing a common bait.


Cross References:

Database
Identifier
Secondary identifier
Qualifier
-

Annotations:

Topic
Text
Figs 1 and S1, table S1
Treatment with DNA mimic poly(I:C) induces the interaction with RIG-I and RIP2.

Participants (11)

Legend:
A
Annotation and Cross Reference  
P
Experimental Parameter   
S
Stoichiometry   
F
Experimental Feature
C
Participant Confidence
#
Name
Links
Primary Identifier
Aliases
Description
Species
Expression system
Experimental role
Biological role
Interactor type
More...
1
EBI-995373
 logo
Interferon beta promoter stimulator protein 1
Virus-induced-signaling adapter
CARD adapter inducing interferon beta

[+5]
Mitochondrial antiviral-signaling protein
Homo sapiens
Organism Details

Accession: EBI-874

Name: human

Description: Homo sapiens

Cross References:

Database
Identifier
Secondary identifier
Qualifier
human

Expressed In Details

Accession:

Name:

-
bait
Experimental role: bait
Accession: EBI-49

Name: bait

Description: bait

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0496
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Molecule experimentally treated to capture its interacting partners.


unspecified role
Biological role: unspecified role
Accession: EBI-77781

Name: unspecified role

Description: unspecified role

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0499
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Role not specified or not applicable to the data.


protein
Interactor type: protein
Accession: EBI-619654

Name: protein

Description: protein

Cross References:
Database
Identifier
Secondary identifier
Qualifier
so
SO:0000358
-
see-also
psi-mi
MI:0326
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
A linear polymer of amino acids joined by peptide bonds in a specific sequence.


A
Participant: Mitochondrial antiviral-signaling protein

Participant: Mitochondrial antiviral-signaling protein

Accession: EBI-6115767

Name: EBI-995373

P
Participant: Mitochondrial antiviral-signaling protein

Participant: Mitochondrial antiviral-signaling protein

Accession: EBI-6115767

Name: EBI-995373

Parameters:

Type
Value
Unit
Base
Exponent
Uncertainty
No records found.


S
Participant: Mitochondrial antiviral-signaling protein

Participant: Mitochondrial antiviral-signaling protein

Accession: EBI-6115767

Name: EBI-995373

Stoichiometry: 0.0

F
Participant: Mitochondrial antiviral-signaling protein

Participant: Mitochondrial antiviral-signaling protein

Accession: EBI-6115767

Name: EBI-995373

Features:

Legend:
A
Annotation and Cross Reference  
Feature Name
Feature Type
Detection Method
Range positions
More...
flag tag
  • ?-?
  • A
    Feature: EBI-6115768

    Feature: flag tag

    Accession: EBI-6115768

    Name: flag tag


    C
    Participant: Mitochondrial antiviral-signaling protein

    Participant: Mitochondrial antiviral-signaling protein

    Accession: EBI-6115767

    Name: EBI-995373

    Confidences:

    Type
    Value
    No records found.

    2
    EBI-1175454
     logo
    KIAA0648
    PDS5
    PIG54

    [+3]
    Sister chromatid cohesion protein PDS5 homolog A
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Sister chromatid cohesion protein PDS5 homolog A

    Participant: Sister chromatid cohesion protein PDS5 homolog A

    Accession: EBI-6115757

    Name: EBI-1175454

    P
    Participant: Sister chromatid cohesion protein PDS5 homolog A

    Participant: Sister chromatid cohesion protein PDS5 homolog A

    Accession: EBI-6115757

    Name: EBI-1175454

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Sister chromatid cohesion protein PDS5 homolog A

    Participant: Sister chromatid cohesion protein PDS5 homolog A

    Accession: EBI-6115757

    Name: EBI-1175454

    Stoichiometry: 0.0

    F
    Participant: Sister chromatid cohesion protein PDS5 homolog A

    Participant: Sister chromatid cohesion protein PDS5 homolog A

    Accession: EBI-6115757

    Name: EBI-1175454

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Sister chromatid cohesion protein PDS5 homolog A

    Participant: Sister chromatid cohesion protein PDS5 homolog A

    Accession: EBI-6115757

    Name: EBI-1175454

    Confidences:

    Type
    Value
    No records found.

    3
    EBI-347552
     logo
    BCL2-associated athanogene 6
    BAG6
    BAG family molecular chaperone regulator 6

    [+5]
    Large proline-rich protein BAG6
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Large proline-rich protein BAG6

    Participant: Large proline-rich protein BAG6

    Accession: EBI-6115758

    Name: EBI-347552

    P
    Participant: Large proline-rich protein BAG6

    Participant: Large proline-rich protein BAG6

    Accession: EBI-6115758

    Name: EBI-347552

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Large proline-rich protein BAG6

    Participant: Large proline-rich protein BAG6

    Accession: EBI-6115758

    Name: EBI-347552

    Stoichiometry: 0.0

    F
    Participant: Large proline-rich protein BAG6

    Participant: Large proline-rich protein BAG6

    Accession: EBI-6115758

    Name: EBI-347552

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Large proline-rich protein BAG6

    Participant: Large proline-rich protein BAG6

    Accession: EBI-6115758

    Name: EBI-347552

    Confidences:

    Type
    Value
    No records found.

    4
    EBI-6115729
     logo
    OAS3
    p100 OAS
    P/OKcl.4
    2'-5'-oligoadenylate synthase 3
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: 2'-5'-oligoadenylate synthase 3

    Participant: 2'-5'-oligoadenylate synthase 3

    Accession: EBI-6115759

    Name: EBI-6115729

    P
    Participant: 2'-5'-oligoadenylate synthase 3

    Participant: 2'-5'-oligoadenylate synthase 3

    Accession: EBI-6115759

    Name: EBI-6115729

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: 2'-5'-oligoadenylate synthase 3

    Participant: 2'-5'-oligoadenylate synthase 3

    Accession: EBI-6115759

    Name: EBI-6115729

    Stoichiometry: 0.0

    F
    Participant: 2'-5'-oligoadenylate synthase 3

    Participant: 2'-5'-oligoadenylate synthase 3

    Accession: EBI-6115759

    Name: EBI-6115729

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: 2'-5'-oligoadenylate synthase 3

    Participant: 2'-5'-oligoadenylate synthase 3

    Accession: EBI-6115759

    Name: EBI-6115729

    Confidences:

    Type
    Value
    No records found.

    5
    EBI-2691157
     logo
    HECT domain-containing protein 3
    HECTD3
    HECT-type E3 ubiquitin transferase HECTD3
    E3 ubiquitin-protein ligase HECTD3
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: E3 ubiquitin-protein ligase HECTD3

    Participant: E3 ubiquitin-protein ligase HECTD3

    Accession: EBI-6115760

    Name: EBI-2691157

    P
    Participant: E3 ubiquitin-protein ligase HECTD3

    Participant: E3 ubiquitin-protein ligase HECTD3

    Accession: EBI-6115760

    Name: EBI-2691157

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: E3 ubiquitin-protein ligase HECTD3

    Participant: E3 ubiquitin-protein ligase HECTD3

    Accession: EBI-6115760

    Name: EBI-2691157

    Stoichiometry: 0.0

    F
    Participant: E3 ubiquitin-protein ligase HECTD3

    Participant: E3 ubiquitin-protein ligase HECTD3

    Accession: EBI-6115760

    Name: EBI-2691157

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: E3 ubiquitin-protein ligase HECTD3

    Participant: E3 ubiquitin-protein ligase HECTD3

    Accession: EBI-6115760

    Name: EBI-2691157

    Confidences:

    Type
    Value
    No records found.

    6
    EBI-995350
     logo
    Probable ATP-dependent RNA helicase DDX58
    Retinoic acid-inducible gene I protein
    RIG-I-like receptor 1

    [+3]
    Antiviral innate immune response receptor RIG-I
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Antiviral innate immune response receptor RIG-I

    Participant: Antiviral innate immune response receptor RIG-I

    Accession: EBI-6115761

    Name: EBI-995350

    P
    Participant: Antiviral innate immune response receptor RIG-I

    Participant: Antiviral innate immune response receptor RIG-I

    Accession: EBI-6115761

    Name: EBI-995350

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Antiviral innate immune response receptor RIG-I

    Participant: Antiviral innate immune response receptor RIG-I

    Accession: EBI-6115761

    Name: EBI-995350

    Stoichiometry: 0.0

    F
    Participant: Antiviral innate immune response receptor RIG-I

    Participant: Antiviral innate immune response receptor RIG-I

    Accession: EBI-6115761

    Name: EBI-995350

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Antiviral innate immune response receptor RIG-I

    Participant: Antiviral innate immune response receptor RIG-I

    Accession: EBI-6115761

    Name: EBI-995350

    Confidences:

    Type
    Value
    No records found.

    7
    EBI-355744
     logo
    Tumor necrosis factor type 2 receptor-associated protein 3
    E3 ubiquitin-protein ligase TRAF2
    RING-type E3 ubiquitin transferase TRAF2

    [+2]
    TNF receptor-associated factor 2
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: TNF receptor-associated factor 2

    Participant: TNF receptor-associated factor 2

    Accession: EBI-6115762

    Name: EBI-355744

    P
    Participant: TNF receptor-associated factor 2

    Participant: TNF receptor-associated factor 2

    Accession: EBI-6115762

    Name: EBI-355744

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: TNF receptor-associated factor 2

    Participant: TNF receptor-associated factor 2

    Accession: EBI-6115762

    Name: EBI-355744

    Stoichiometry: 0.0

    F
    Participant: TNF receptor-associated factor 2

    Participant: TNF receptor-associated factor 2

    Accession: EBI-6115762

    Name: EBI-355744

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: TNF receptor-associated factor 2

    Participant: TNF receptor-associated factor 2

    Accession: EBI-6115762

    Name: EBI-355744

    Confidences:

    Type
    Value
    No records found.

    8
    EBI-359276
     logo
    Interleukin-1 signal transducer
    RING finger protein 85
    E3 ubiquitin-protein ligase TRAF6

    [+3]
    TNF receptor-associated factor 6
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: TNF receptor-associated factor 6

    Participant: TNF receptor-associated factor 6

    Accession: EBI-6115763

    Name: EBI-359276

    P
    Participant: TNF receptor-associated factor 6

    Participant: TNF receptor-associated factor 6

    Accession: EBI-6115763

    Name: EBI-359276

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: TNF receptor-associated factor 6

    Participant: TNF receptor-associated factor 6

    Accession: EBI-6115763

    Name: EBI-359276

    Stoichiometry: 0.0

    F
    Participant: TNF receptor-associated factor 6

    Participant: TNF receptor-associated factor 6

    Accession: EBI-6115763

    Name: EBI-359276

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: TNF receptor-associated factor 6

    Participant: TNF receptor-associated factor 6

    Accession: EBI-6115763

    Name: EBI-359276

    Confidences:

    Type
    Value
    No records found.

    9
    EBI-1048119
     logo
    RING-type E3 ubiquitin transferase E4 A
    UBE4A
    KIAA0126
    Ubiquitin conjugation factor E4 A
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Ubiquitin conjugation factor E4 A

    Participant: Ubiquitin conjugation factor E4 A

    Accession: EBI-6115764

    Name: EBI-1048119

    P
    Participant: Ubiquitin conjugation factor E4 A

    Participant: Ubiquitin conjugation factor E4 A

    Accession: EBI-6115764

    Name: EBI-1048119

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Ubiquitin conjugation factor E4 A

    Participant: Ubiquitin conjugation factor E4 A

    Accession: EBI-6115764

    Name: EBI-1048119

    Stoichiometry: 0.0

    F
    Participant: Ubiquitin conjugation factor E4 A

    Participant: Ubiquitin conjugation factor E4 A

    Accession: EBI-6115764

    Name: EBI-1048119

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Ubiquitin conjugation factor E4 A

    Participant: Ubiquitin conjugation factor E4 A

    Accession: EBI-6115764

    Name: EBI-1048119

    Confidences:

    Type
    Value
    No records found.

    10
    EBI-1057697
     logo
    Transcription factor ISGF-3 components p91/p84
    STAT1
    Signal transducer and activator of transcription 1-alpha/beta
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Signal transducer and activator of transcription 1-alpha/beta

    Participant: Signal transducer and activator of transcription 1-alpha/beta

    Accession: EBI-6115765

    Name: EBI-1057697

    P
    Participant: Signal transducer and activator of transcription 1-alpha/beta

    Participant: Signal transducer and activator of transcription 1-alpha/beta

    Accession: EBI-6115765

    Name: EBI-1057697

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Signal transducer and activator of transcription 1-alpha/beta

    Participant: Signal transducer and activator of transcription 1-alpha/beta

    Accession: EBI-6115765

    Name: EBI-1057697

    Stoichiometry: 0.0

    F
    Participant: Signal transducer and activator of transcription 1-alpha/beta

    Participant: Signal transducer and activator of transcription 1-alpha/beta

    Accession: EBI-6115765

    Name: EBI-1057697

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Signal transducer and activator of transcription 1-alpha/beta

    Participant: Signal transducer and activator of transcription 1-alpha/beta

    Accession: EBI-6115765

    Name: EBI-1057697

    Confidences:

    Type
    Value
    No records found.

    11
    EBI-358522
     logo
    RIP-like-interacting CLARP kinase
    Receptor-interacting protein 2
    CARD-containing interleukin-1 beta-converting enzyme-associated kinase

    [+6]
    Receptor-interacting serine/threonine-protein kinase 2
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Receptor-interacting serine/threonine-protein kinase 2

    Participant: Receptor-interacting serine/threonine-protein kinase 2

    Accession: EBI-6115766

    Name: EBI-358522

    P
    Participant: Receptor-interacting serine/threonine-protein kinase 2

    Participant: Receptor-interacting serine/threonine-protein kinase 2

    Accession: EBI-6115766

    Name: EBI-358522

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Receptor-interacting serine/threonine-protein kinase 2

    Participant: Receptor-interacting serine/threonine-protein kinase 2

    Accession: EBI-6115766

    Name: EBI-358522

    Stoichiometry: 0.0

    F
    Participant: Receptor-interacting serine/threonine-protein kinase 2

    Participant: Receptor-interacting serine/threonine-protein kinase 2

    Accession: EBI-6115766

    Name: EBI-358522

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Receptor-interacting serine/threonine-protein kinase 2

    Participant: Receptor-interacting serine/threonine-protein kinase 2

    Accession: EBI-6115766

    Name: EBI-358522

    Confidences:

    Type
    Value
    No records found.


    Graphical Representation of Experimental Features