Please wait...
loading   Loading...
What is this view?

Binary interactions

In this tab, we display the list of interactions that you have selected using one of our search features. Despite the fact that our data are annotated to accurately reflect the interactions reported in scientific literature, the data is shown in this view as binary interactions. Whenever the data was reported as a co-complex involving more than two molecules, we store it as such in the IntAct database and post-process it so the portal can show it as binary interaction. This post-processing is the Spoke Expansion model (connects bait to all preys):



sourceExp

At any moment you can choose to display the expansion column in this view in order to see which interaction are spoke expanded and which are not.

Description of what has changed

  • We have added more download options to allow users to retrieve their interaction set using more standard formats such as PSI-MI XML and PSIMITAB (version 2.5, 2.6 or 2.7) but also XGMML, RDF and Biopax (level 2 and 3).
  • We have now four different table views : minimal(molecule names and interaction AC), basic (minimal + molecule links, interaction detection method, negative), standard(minimal + molecule species, confidences, publication details, experiment details), expanded (standard + more experiment details) and complete (all mitab 2.7 columns).

Configuring the view to your need"

In the header of the interaction table you will find a button: ‘Change Column Display’ that will show you all the columns/Table views available and allow you to update the current selected set.

Downloading the data into Standard formats"

In the header of the interaction table you will find a drop down list that contains all the formats currently supported when downloading the interaction data. Select one of them and click the export button next to the list. Please note that PSI-MI XML is only available when the interaction set is no bigger than 1000 interactions.

Opening the interaction details"

Clicking on the magnifying glass in the first column of the interaction table will open the details of the corresponding interaction in the Interaction Details tab, giving you access to more details of the manually curated record.

What is this view?

Browsing (Browse Tab)

This tab is meant to give you access to more content based on the currently selected set of interactions. Please note that linking to third party resources will only include up to 200 molecules , if you exceed this number you will see the warning icon (This number has been reduced to 125 molecules for mRNA expression). Now let’s look at the features available to you:

Limiting the scope of the current dataset with the Uniprot Taxonomy ontology

Allows users to browse the Uniprot Taxonomy hierarchy as a tree and select terms in order to narrow down their dataset. Once a term is selected, you are taken back to the interaction tab to review your dataset.

Limiting the scope of the current dataset with the GO ontology

Allows users to browse the GO hierarchy as a tree and select terms in order to narrow down their dataset. Once a term is selected, you are taken back to the interaction tab to review your dataset.

Limiting the scope of the current dataset with the ChEBI ontology

Allows users to browse the ChEBI hierarchy as a tree and select terms in order to narrow down their dataset. Once a term is selected, you are taken back to the interaction tab to review your dataset.

Bulk linking to third party resources by using involved proteins

  • Proteins by Reactome pathway: Sends your proteins to the Reactome SkyPainter that will show you the pathways in which these molecules are know to play a role.
  • Proteins by Chromosomal location: Sends your list of proteins to Ensembl’s Karyotype viewer and overlays the proteins on the chromosomes.
  • Proteins by mRNA expression: Sends your set of proteins to the ArrayExpress Atlas that will show the known gene expression based on experimental studies.
What is this view?

Searching Interactions (Search Tab)

As you can see in this tab we are now trying to give you more targeted choice to do your queries, please note that the examples provided in this tab are live links so you can simply click them to see the resulting interactions sets.

Using the Quick Search

In this search panel you are free to type anything that might relate to interactions, whether it is properties of their interactor (gene name, identifiers, GO term…) or more specific to the interaction like publication, authors, experimental detection method, ...

Some examples:

  • Try the query: imatinib
    This is a drug for which we have curated a number of interactions.
    Once you press the search button you should be taken to the Interaction Tab that lists 130 binary interactions.
    If you want to construct more complex queries we recommend you take a look at the Molecular Interaction Query Language, accessible from the quick search panel.
  • Try the query: species:yeast AND type:"direct interaction"
    This query selects all interactions involving yeast interactors that have been shown to have direct interactions. If you customize the column display of the interaction tab, you will see that not only “direct interaction” have been selected but also children terms in the PSI-MI ontology.

Using the Ontology Search

Open the Rearch Tab. This panel is specialised to give you an easy access to ontology search. So far you can search on 4 ontologies:

  • Gene Ontology
  • InterPro
  • PSI-MI
  • ChEBI

Whenever you start typing a query in this search panel, the system will search as you type and propose a list of matching controlled vocabulary terms. You can then select one of them and select matching interactions.

For example, type: cancer
You will be presented with a few choices, please note that each term is followed by the count of matching interactions in the IntAct database.

Select a term with the mouse or using the keyboard cursor keys and you will be taken to the interaction tab.

Searching the Compound chemical structure

In this panel you will be able to draw all or part of a chemical structure and search for chemical compounds. If you get any matched, you can then see all interactions involving them.

First you have to open up the chemical search panel so that the applet can load, it might take a few seconds. Then you can start drawing your structure, for instance:

Once you have drawn your structure, select Similarity and press Search. You should be presented with a list of matching compound. Now choose one molecule and click the link: IntAct interactions. You will be taken to the interaction tab to review the data.

Complex Expansion

Binary interactions generated by co-complex expansion

Why should you care about complex expansion ?

Some experimental methods such as Tandem Affinity Purification do generate molecular interactions that can involve more than 2 molecules. Despite the fact that IntAct curation team do capture the molecular interaction as they were reported in the corresponding experiment, when you search using the intact web site, the results of your query is always shown as set of binary interactions (i.e. 2 molecules). We would like to draw your attention on the fact that whenever the reported interaction was a co-complex we do apply an expansion algorithm that transform this n-ary interaction into a set of binary interactions. While none of these agorithms is perfect and will very likely generate some false positive interactions, it is useful to present the data in a consistent manner. Bear in mind that we will strive to differentiate in the search results which interactions are a real experimental binary from expanded ones.

Existing expansion algorithm

There are several known algorithm allowing to transform an n-ary interaction into a set of binaries. The illustration below present the two well known expansion model and illustrates why they can be incorrect.



sourceExp

  • Spoke expansion: Links the bait molecule to all prey molecules. If N is the count of molecule in the complex, it generated N-1 binary interactions.
  • Matrix expansion: Links all molecule to all other molecule present in the complex. If N is the count of molecule in the complex, it generated (N*(N-1))/2 binary interactions.

Now the issue (as illustrated at the bottom right of the diagram above) with these two models lies in the fact that the real complex might not be articulated around the experimental bait but instead, this bait might be linked to a smaller complex, hence most binary interaction generated by spoke and matrix expansion result in false positive.



PSICQUIC

How is the number of interactions in other databases obtained?

PSICQUIC is a standard way to access molecular interaction databases across which it repeats the same query. The number of databases providing data may vary, depending on the status of their services and only those that are active are used in this query. By clicking on the number of interactions you will be redirected to the PSICQUIC View, where you can browse the results in those other resources.

The services currently active are:

Check the PSICQUIC site for more information.

IMEx

What is the significance of the IMEx dataset?"

IMEx is a network of databases which have agreed to supply a non-redundant set of data expertly manually annotated to the same consistent detailed standard which, as such, represents a high-quality subset of the data each individually provides. The number of databases providing data may vary, depending on the status of their services and only those that are active are used in this query. By clicking on the number of interactions you will be redirected to the IMEx View, where you can browse the results in those other resources.

The services currently active are:

Check the IMEx site for more information.

What is this view?

Representation of Experimental Features

This section shows the graphical representation of experimental features, where each participant is represented as a white rectangle with a black border and a line for each hundredth amino acid. All available features are attached to their associated participant and their categories are represented in the right side of the legend. The left side of the legend dynamically shows the range statuses occuring in the shown interaction. These are the possible range statuses:

sourceExp

Interacting with the widget

Hover over a feature to see more information in a tooltip.
sourceExp

To display a single interacting region click on it and click again to display all interacting regions.
Displaying all interacting regionsDisplaying one interacting region
sourceExpsourceExp
What is this view?

Dynamic molecular interaction data

This section shows the graphical representation of dynamic molecular interactions. By default it displays all the interactions from one experiment using radio buttons to allow users to highlight interactions in different variable conditions.

Publication

PubMed Id: 31980649

Journal: Nature Communications

Year of Publication: 2019

Title: Extensive Rewiring of the EGFR Network in Colorectal Cancer Cells Expressing Transforming Levels of KRASG13D

Author List: Kennedy SA, Jarboui MA, Srihari S, Raso C, Bryan K, Dernayka L, Charitou T, Bernal-Llinares M, Herrera-Montavez C, Krstic A, Matallanas D, Kotlyar M, Jurisica I, Curak J, Wong V, Stagljar I, LeBihan T, Imrie L, Pillai P, Lynn MA, Fasterius E, Al-Khalili Szigyarto C, Breen J, Kiel C, Serrano L, Rauch N, Rukhlenko O, Kholodenko BN, Iglesias-Martinez LF, Ryan CJ, Pilkington R, Cammareri P, Sansom O, Shave S, Auer M, Horn N, Klose F, Ueffing M, Boldt K, Lynn DJ, Kolch W

Cross References:

Annotations:

Topic
Text
Cancer - Interactions investigated in the context of cancer

Experiment (91 interactions)

Accession: EBI-25385328

Name: kennedy-2019-3

Host organism: human HKE-3 cell line

Organism: human HKE-3 cell line

Accession: EBI-22274219

Name: human-hke3

Description: human HKE-3 cell line

Interaction Detection Method: anti tag coip

Interaction detection method: anti tag coip
Accession: EBI-90

Name: anti tag coip

Description: anti tag coimmunoprecipitation

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0007
-
identity
pubmed
7708014
-
primary-reference


Annotations:
Topic
Text
definition
A specific antibody for the molecule of interest is not available, therefore the bait protein is expressed as a hybrid protein fused to a tag peptide/protein for which efficient and specific antibodies or a specific ligand are available.


Participant Identification Method: sequence tag

Participant identification method: sequence tag
Accession: EBI-81

Name: sequence tag

Description: sequence tag identification

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0102
-
identity
pubmed
10967324
-
primary-reference
pubmed
11752590
-
primary-reference
pubmed
11805837
-
primary-reference


Annotations:
Topic
Text
definition
This approach leads to protein identification by combining mass measurement and short amino acid sequence information obtained by tandem mass spectrometry. This information is then used to automatically find the best match in a sequence database. A mixture of peptides derived from a protease digestion is analysed by nanoelectrospray LC-MS/MS (Liquid Chromatography Tandem Mass Spectrometer or nanoESI MS/MS) mass spectrometry. Electrospray mass spectrometry cannot be applied to dilute samples and is affected by high salt. As a consequence peptides, normally extracted from acrylamide gels by in situ proteolysis, are desalted and concentrated on a microcolumn followed by elution into a capillary used for nanoelectrospray tandem mass spectrometry. A first mass spectrum (Normal mass spectrum or Q1 mass spectrum) gives information about the masses of all the peptides. Peptides observed in the normal mass spectrum are isolated in turn and dissociated into fragments by collision with gas molecules within the mass spectrometer. Some of the fragments obtained from a peptide constitute a nested set, differing by one amino acid, and the mass difference between them allows assignment of a partial sequence. The masses of the fragments define the position of the partial sequence in the peptide. Together with the cleavage specificity of the protease used to cleave the protein, and mass information such sequence tag provides much higher search specificity to match the a database entry. The procedure is repeated with several peptides from the digest, resulting in multiple identifications of the same protein or identification of several proteins from the peptide mixture. Unknown proteins can easily be identified by using the high specificity of the peptide sequence tag for searches in most sequence databases including EST or genome databases.


Cross References:

Annotations:

Topic
Text
The entry is part of the PrimesDB project, an IMEx observer member.
95 bait proteins were selected based on a highly curated EGFR signaling. The baits were expressed as FLAG-tagged proteins carefully titrating transfection to achieve a similar expression level in both cell lines (HCT116 and HKE3). Baits were immunoprecipitated and associated prey proteins were identified by high- resolution Orbitrap qMS. We defined preys as being “rewired” in EGFRNet mtKRAS-Lo (HKE3 cells), if the abundance of the prey protein in the pull-down was significantly different (P <= 0.05) compared to EGFRNet mtKRAS-Hi (HCT116 cells) and the significance A value for the prey protein was also <= 0.05. Interactions that were identified in only one EGFRNet, but where prey abundance was subsequently not found to be statistically significantly different in the respective bait-prey complexes in the two cell lines were not considered as rewired preys. (Only for 91 baits interactions were found).
imex curation
walter.kolch@ucd.ie,david.lynn@sahmri.com,karsten.boldt@uni-tuebingen.de
2019-12-05: Submitted by David J. Lynn, EMBL Australia Group, Adelaide, Australia

Interaction

Accession: EBI-25395492

Name: ywhae-arhgef19-2

Description: -

Type: association

Interaction type: association
Accession: EBI-1813191

Name: association

Description: association

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0914
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Interaction between molecules that may participate in formation of one, but possibly more, physical complexes. Often describes a set of molecules that are co-purified in a single pull-down or coimmunoprecipitation but might participate in formation of distinct physical complexes sharing a common bait.


Cross References:

Database
Identifier
Secondary identifier
Qualifier
-
-

Annotations:

Topic
Text
Suppl Data 2, 4 and 5

Participants (27)

Legend:
A
Annotation and Cross Reference  
P
Experimental Parameter   
S
Stoichiometry   
F
Experimental Feature
C
Participant Confidence
pp12pp
#
Name
Links
Primary Identifier
Aliases
Description
Species
Expression system
Experimental role
Biological role
Interactor type
More...
pp12pp
1
EBI-356498
 logo
YWHAE
14-3-3 protein epsilon
Homo sapiens
Organism Details

Accession: EBI-874

Name: human

Description: Homo sapiens

Cross References:

Database
Identifier
Secondary identifier
Qualifier
human

Expressed In Details

Accession:

Name:

-
bait
Experimental role: bait
Accession: EBI-49

Name: bait

Description: bait

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0496
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Molecule experimentally treated to capture its interacting partners.


unspecified role
Biological role: unspecified role
Accession: EBI-77781

Name: unspecified role

Description: unspecified role

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0499
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Role not specified or not applicable to the data.


protein
Interactor type: protein
Accession: EBI-619654

Name: protein

Description: protein

Cross References:
Database
Identifier
Secondary identifier
Qualifier
so
SO:0000358
-
see-also
psi-mi
MI:0326
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
A linear polymer of amino acids joined by peptide bonds in a specific sequence.


A
Participant: 14-3-3 protein epsilon

Participant: 14-3-3 protein epsilon

Accession: EBI-25395496

Name: EBI-356498

P
Participant: 14-3-3 protein epsilon

Participant: 14-3-3 protein epsilon

Accession: EBI-25395496

Name: EBI-356498

Parameters:

Type
Value
Unit
Base
Exponent
Uncertainty
No records found.


S
Participant: 14-3-3 protein epsilon

Participant: 14-3-3 protein epsilon

Accession: EBI-25395496

Name: EBI-356498

Stoichiometry: 0.0

F
Participant: 14-3-3 protein epsilon

Participant: 14-3-3 protein epsilon

Accession: EBI-25395496

Name: EBI-356498

Features:

Legend:
A
Annotation and Cross Reference  
Feature Name
Feature Type
Detection Method
Range positions
More...
flag tag
  • n-n
  • A
    Feature: EBI-25395498

    Feature: flag tag

    Accession: EBI-25395498

    Name: flag tag


    C
    Participant: 14-3-3 protein epsilon

    Participant: 14-3-3 protein epsilon

    Accession: EBI-25395496

    Name: EBI-356498

    Confidences:

    Type
    Value
    No records found.

    2
    EBI-727407
     logo
    Protein Nezha
    CAMSAP3
    KIAA1543
    Calmodulin-regulated spectrin-associated protein 3
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Calmodulin-regulated spectrin-associated protein 3

    Participant: Calmodulin-regulated spectrin-associated protein 3

    Accession: EBI-25395500

    Name: EBI-727407

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 6.59

    P
    Participant: Calmodulin-regulated spectrin-associated protein 3

    Participant: Calmodulin-regulated spectrin-associated protein 3

    Accession: EBI-25395500

    Name: EBI-727407

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Calmodulin-regulated spectrin-associated protein 3

    Participant: Calmodulin-regulated spectrin-associated protein 3

    Accession: EBI-25395500

    Name: EBI-727407

    Stoichiometry: 0.0

    F
    Participant: Calmodulin-regulated spectrin-associated protein 3

    Participant: Calmodulin-regulated spectrin-associated protein 3

    Accession: EBI-25395500

    Name: EBI-727407

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Calmodulin-regulated spectrin-associated protein 3

    Participant: Calmodulin-regulated spectrin-associated protein 3

    Accession: EBI-25395500

    Name: EBI-727407

    Confidences:

    Type
    Value
    No records found.

    3
    EBI-3258000
     logo
    Cell migration-inducing protein 23
    TBC1D7
    TBC7

    [+1]
    TBC1 domain family member 7
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: TBC1 domain family member 7

    Participant: TBC1 domain family member 7

    Accession: EBI-25395503

    Name: EBI-3258000

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 7.49

    P
    Participant: TBC1 domain family member 7

    Participant: TBC1 domain family member 7

    Accession: EBI-25395503

    Name: EBI-3258000

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: TBC1 domain family member 7

    Participant: TBC1 domain family member 7

    Accession: EBI-25395503

    Name: EBI-3258000

    Stoichiometry: 0.0

    F
    Participant: TBC1 domain family member 7

    Participant: TBC1 domain family member 7

    Accession: EBI-25395503

    Name: EBI-3258000

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: TBC1 domain family member 7

    Participant: TBC1 domain family member 7

    Accession: EBI-25395503

    Name: EBI-3258000

    Confidences:

    Type
    Value
    No records found.

    4
    EBI-7954108
     logo
    TANC2
    KIAA1148
    KIAA1636

    [+1]
    Protein TANC2
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Protein TANC2

    Participant: Protein TANC2

    Accession: EBI-25395506

    Name: EBI-7954108

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 7.74

    P
    Participant: Protein TANC2

    Participant: Protein TANC2

    Accession: EBI-25395506

    Name: EBI-7954108

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Protein TANC2

    Participant: Protein TANC2

    Accession: EBI-25395506

    Name: EBI-7954108

    Stoichiometry: 0.0

    F
    Participant: Protein TANC2

    Participant: Protein TANC2

    Accession: EBI-25395506

    Name: EBI-7954108

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Protein TANC2

    Participant: Protein TANC2

    Accession: EBI-25395506

    Name: EBI-7954108

    Confidences:

    Type
    Value
    No records found.

    5
    EBI-7221344
     logo
    EPN3
    EPS-15-interacting protein 3
    Epsin-3
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Epsin-3

    Participant: Epsin-3

    Accession: EBI-25395509

    Name: EBI-7221344

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 12.05

    P
    Participant: Epsin-3

    Participant: Epsin-3

    Accession: EBI-25395509

    Name: EBI-7221344

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Epsin-3

    Participant: Epsin-3

    Accession: EBI-25395509

    Name: EBI-7221344

    Stoichiometry: 0.0

    F
    Participant: Epsin-3

    Participant: Epsin-3

    Accession: EBI-25395509

    Name: EBI-7221344

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Epsin-3

    Participant: Epsin-3

    Accession: EBI-25395509

    Name: EBI-7221344

    Confidences:

    Type
    Value
    No records found.

    6
    EBI-2799206
     logo
    CCDC123
    Coiled-coil domain-containing protein 123
    CEP89

    [+1]
    Centrosomal protein of 89 kDa
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Centrosomal protein of 89 kDa

    Participant: Centrosomal protein of 89 kDa

    Accession: EBI-25395512

    Name: EBI-2799206

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 9.03

    P
    Participant: Centrosomal protein of 89 kDa

    Participant: Centrosomal protein of 89 kDa

    Accession: EBI-25395512

    Name: EBI-2799206

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Centrosomal protein of 89 kDa

    Participant: Centrosomal protein of 89 kDa

    Accession: EBI-25395512

    Name: EBI-2799206

    Stoichiometry: 0.0

    F
    Participant: Centrosomal protein of 89 kDa

    Participant: Centrosomal protein of 89 kDa

    Accession: EBI-25395512

    Name: EBI-2799206

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Centrosomal protein of 89 kDa

    Participant: Centrosomal protein of 89 kDa

    Accession: EBI-25395512

    Name: EBI-2799206

    Confidences:

    Type
    Value
    No records found.

    7
    EBI-717962
     logo
    HECT-type E3 ubiquitin transferase NED4L
    Nedd4-2
    NEDD4.2

    [+3]
    E3 ubiquitin-protein ligase NEDD4-like
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: E3 ubiquitin-protein ligase NEDD4-like

    Participant: E3 ubiquitin-protein ligase NEDD4-like

    Accession: EBI-25395515

    Name: EBI-717962

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 5.75

    P
    Participant: E3 ubiquitin-protein ligase NEDD4-like

    Participant: E3 ubiquitin-protein ligase NEDD4-like

    Accession: EBI-25395515

    Name: EBI-717962

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: E3 ubiquitin-protein ligase NEDD4-like

    Participant: E3 ubiquitin-protein ligase NEDD4-like

    Accession: EBI-25395515

    Name: EBI-717962

    Stoichiometry: 0.0

    F
    Participant: E3 ubiquitin-protein ligase NEDD4-like

    Participant: E3 ubiquitin-protein ligase NEDD4-like

    Accession: EBI-25395515

    Name: EBI-717962

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: E3 ubiquitin-protein ligase NEDD4-like

    Participant: E3 ubiquitin-protein ligase NEDD4-like

    Accession: EBI-25395515

    Name: EBI-717962

    Confidences:

    Type
    Value
    No records found.

    8
    EBI-1790529
     logo
    THAP11
    HRIHFB2206
    THAP domain-containing protein 11
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: THAP domain-containing protein 11

    Participant: THAP domain-containing protein 11

    Accession: EBI-25395518

    Name: EBI-1790529

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 32.03

    P
    Participant: THAP domain-containing protein 11

    Participant: THAP domain-containing protein 11

    Accession: EBI-25395518

    Name: EBI-1790529

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: THAP domain-containing protein 11

    Participant: THAP domain-containing protein 11

    Accession: EBI-25395518

    Name: EBI-1790529

    Stoichiometry: 0.0

    F
    Participant: THAP domain-containing protein 11

    Participant: THAP domain-containing protein 11

    Accession: EBI-25395518

    Name: EBI-1790529

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: THAP domain-containing protein 11

    Participant: THAP domain-containing protein 11

    Accession: EBI-25395518

    Name: EBI-1790529

    Confidences:

    Type
    Value
    No records found.

    9
    EBI-11017708
     logo
    TTC17
    Tetratricopeptide repeat protein 17
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Tetratricopeptide repeat protein 17

    Participant: Tetratricopeptide repeat protein 17

    Accession: EBI-25395521

    Name: EBI-11017708

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 6.05

    P
    Participant: Tetratricopeptide repeat protein 17

    Participant: Tetratricopeptide repeat protein 17

    Accession: EBI-25395521

    Name: EBI-11017708

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Tetratricopeptide repeat protein 17

    Participant: Tetratricopeptide repeat protein 17

    Accession: EBI-25395521

    Name: EBI-11017708

    Stoichiometry: 0.0

    F
    Participant: Tetratricopeptide repeat protein 17

    Participant: Tetratricopeptide repeat protein 17

    Accession: EBI-25395521

    Name: EBI-11017708

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Tetratricopeptide repeat protein 17

    Participant: Tetratricopeptide repeat protein 17

    Accession: EBI-25395521

    Name: EBI-11017708

    Confidences:

    Type
    Value
    No records found.

    10
    EBI-3938822
     logo
    PHACTR4
    PRO2963
    Phosphatase and actin regulator 4
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Phosphatase and actin regulator 4

    Participant: Phosphatase and actin regulator 4

    Accession: EBI-25395524

    Name: EBI-3938822

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 9.26

    P
    Participant: Phosphatase and actin regulator 4

    Participant: Phosphatase and actin regulator 4

    Accession: EBI-25395524

    Name: EBI-3938822

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Phosphatase and actin regulator 4

    Participant: Phosphatase and actin regulator 4

    Accession: EBI-25395524

    Name: EBI-3938822

    Stoichiometry: 0.0

    F
    Participant: Phosphatase and actin regulator 4

    Participant: Phosphatase and actin regulator 4

    Accession: EBI-25395524

    Name: EBI-3938822

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Phosphatase and actin regulator 4

    Participant: Phosphatase and actin regulator 4

    Accession: EBI-25395524

    Name: EBI-3938822

    Confidences:

    Type
    Value
    No records found.

    11
    EBI-7799822
     logo
    Ephexin-2
    ARHGEF19
    Rho guanine nucleotide exchange factor 19
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Rho guanine nucleotide exchange factor 19

    Participant: Rho guanine nucleotide exchange factor 19

    Accession: EBI-25395527

    Name: EBI-7799822

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 12.21

    P
    Participant: Rho guanine nucleotide exchange factor 19

    Participant: Rho guanine nucleotide exchange factor 19

    Accession: EBI-25395527

    Name: EBI-7799822

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Rho guanine nucleotide exchange factor 19

    Participant: Rho guanine nucleotide exchange factor 19

    Accession: EBI-25395527

    Name: EBI-7799822

    Stoichiometry: 0.0

    F
    Participant: Rho guanine nucleotide exchange factor 19

    Participant: Rho guanine nucleotide exchange factor 19

    Accession: EBI-25395527

    Name: EBI-7799822

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Rho guanine nucleotide exchange factor 19

    Participant: Rho guanine nucleotide exchange factor 19

    Accession: EBI-25395527

    Name: EBI-7799822

    Confidences:

    Type
    Value
    No records found.

    12
    EBI-3893327
     logo
    FAM117B
    ALS2CR13
    Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 13 protein
    Protein FAM117B
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Protein FAM117B

    Participant: Protein FAM117B

    Accession: EBI-25395530

    Name: EBI-3893327

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 10.14

    P
    Participant: Protein FAM117B

    Participant: Protein FAM117B

    Accession: EBI-25395530

    Name: EBI-3893327

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Protein FAM117B

    Participant: Protein FAM117B

    Accession: EBI-25395530

    Name: EBI-3893327

    Stoichiometry: 0.0

    F
    Participant: Protein FAM117B

    Participant: Protein FAM117B

    Accession: EBI-25395530

    Name: EBI-3893327

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Protein FAM117B

    Participant: Protein FAM117B

    Accession: EBI-25395530

    Name: EBI-3893327

    Confidences:

    Type
    Value
    No records found.

    13
    EBI-2556221
     logo
    ANKRD15
    KANK
    KIAA0172

    [+3]
    KN motif and ankyrin repeat domain-containing protein 1
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: KN motif and ankyrin repeat domain-containing protein 1

    Participant: KN motif and ankyrin repeat domain-containing protein 1

    Accession: EBI-25395533

    Name: EBI-2556221

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    -

    Annotations:

    Topic
    Text
    Prey abundance was significantly different between HCT116 and HKE3 cells.
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 10.41
    SILAC Ratio (Prey in HCT116 IP / Prey in HKE3 IP): 0.34

    P
    Participant: KN motif and ankyrin repeat domain-containing protein 1

    Participant: KN motif and ankyrin repeat domain-containing protein 1

    Accession: EBI-25395533

    Name: EBI-2556221

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: KN motif and ankyrin repeat domain-containing protein 1

    Participant: KN motif and ankyrin repeat domain-containing protein 1

    Accession: EBI-25395533

    Name: EBI-2556221

    Stoichiometry: 0.0

    F
    Participant: KN motif and ankyrin repeat domain-containing protein 1

    Participant: KN motif and ankyrin repeat domain-containing protein 1

    Accession: EBI-25395533

    Name: EBI-2556221

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: KN motif and ankyrin repeat domain-containing protein 1

    Participant: KN motif and ankyrin repeat domain-containing protein 1

    Accession: EBI-25395533

    Name: EBI-2556221

    Confidences:

    Type
    Value
    No records found.

    14
    EBI-2556193
     logo
    ANKRD25
    KIAA1518
    MXRA3

    [+5]
    KN motif and ankyrin repeat domain-containing protein 2
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: KN motif and ankyrin repeat domain-containing protein 2

    Participant: KN motif and ankyrin repeat domain-containing protein 2

    Accession: EBI-25395539

    Name: EBI-2556193

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 10.41

    P
    Participant: KN motif and ankyrin repeat domain-containing protein 2

    Participant: KN motif and ankyrin repeat domain-containing protein 2

    Accession: EBI-25395539

    Name: EBI-2556193

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: KN motif and ankyrin repeat domain-containing protein 2

    Participant: KN motif and ankyrin repeat domain-containing protein 2

    Accession: EBI-25395539

    Name: EBI-2556193

    Stoichiometry: 0.0

    F
    Participant: KN motif and ankyrin repeat domain-containing protein 2

    Participant: KN motif and ankyrin repeat domain-containing protein 2

    Accession: EBI-25395539

    Name: EBI-2556193

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: KN motif and ankyrin repeat domain-containing protein 2

    Participant: KN motif and ankyrin repeat domain-containing protein 2

    Accession: EBI-25395539

    Name: EBI-2556193

    Confidences:

    Type
    Value
    No records found.

    15
    EBI-2871535
     logo
    DENND4C
    C9orf55
    C9orf55B
    DENN domain-containing protein 4C
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: DENN domain-containing protein 4C

    Participant: DENN domain-containing protein 4C

    Accession: EBI-25395542

    Name: EBI-2871535

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 5.61

    P
    Participant: DENN domain-containing protein 4C

    Participant: DENN domain-containing protein 4C

    Accession: EBI-25395542

    Name: EBI-2871535

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: DENN domain-containing protein 4C

    Participant: DENN domain-containing protein 4C

    Accession: EBI-25395542

    Name: EBI-2871535

    Stoichiometry: 0.0

    F
    Participant: DENN domain-containing protein 4C

    Participant: DENN domain-containing protein 4C

    Accession: EBI-25395542

    Name: EBI-2871535

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: DENN domain-containing protein 4C

    Participant: DENN domain-containing protein 4C

    Accession: EBI-25395542

    Name: EBI-2871535

    Confidences:

    Type
    Value
    No records found.

    16
    EBI-5278370
     logo
    LPIN1
    KIAA0188
    Lipin-1
    Phosphatidate phosphatase LPIN1
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Phosphatidate phosphatase LPIN1

    Participant: Phosphatidate phosphatase LPIN1

    Accession: EBI-25395545

    Name: EBI-5278370

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 6.07

    P
    Participant: Phosphatidate phosphatase LPIN1

    Participant: Phosphatidate phosphatase LPIN1

    Accession: EBI-25395545

    Name: EBI-5278370

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Phosphatidate phosphatase LPIN1

    Participant: Phosphatidate phosphatase LPIN1

    Accession: EBI-25395545

    Name: EBI-5278370

    Stoichiometry: 0.0

    F
    Participant: Phosphatidate phosphatase LPIN1

    Participant: Phosphatidate phosphatase LPIN1

    Accession: EBI-25395545

    Name: EBI-5278370

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Phosphatidate phosphatase LPIN1

    Participant: Phosphatidate phosphatase LPIN1

    Accession: EBI-25395545

    Name: EBI-5278370

    Confidences:

    Type
    Value
    No records found.

    17
    EBI-1052944
     logo
    Chromatin assembly factor I p60 subunit
    M-phase phosphoprotein 7
    CHAF1B

    [+4]
    Chromatin assembly factor 1 subunit B
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Chromatin assembly factor 1 subunit B

    Participant: Chromatin assembly factor 1 subunit B

    Accession: EBI-25395548

    Name: EBI-1052944

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 8.22

    P
    Participant: Chromatin assembly factor 1 subunit B

    Participant: Chromatin assembly factor 1 subunit B

    Accession: EBI-25395548

    Name: EBI-1052944

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Chromatin assembly factor 1 subunit B

    Participant: Chromatin assembly factor 1 subunit B

    Accession: EBI-25395548

    Name: EBI-1052944

    Stoichiometry: 0.0

    F
    Participant: Chromatin assembly factor 1 subunit B

    Participant: Chromatin assembly factor 1 subunit B

    Accession: EBI-25395548

    Name: EBI-1052944

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Chromatin assembly factor 1 subunit B

    Participant: Chromatin assembly factor 1 subunit B

    Accession: EBI-25395548

    Name: EBI-1052944

    Confidences:

    Type
    Value
    No records found.

    18
    EBI-306940
     logo
    Protein AS1
    YWHAH
    YWHA1
    14-3-3 protein eta
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: 14-3-3 protein eta

    Participant: 14-3-3 protein eta

    Accession: EBI-25395551

    Name: EBI-306940

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 7.11

    P
    Participant: 14-3-3 protein eta

    Participant: 14-3-3 protein eta

    Accession: EBI-25395551

    Name: EBI-306940

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: 14-3-3 protein eta

    Participant: 14-3-3 protein eta

    Accession: EBI-25395551

    Name: EBI-306940

    Stoichiometry: 0.0

    F
    Participant: 14-3-3 protein eta

    Participant: 14-3-3 protein eta

    Accession: EBI-25395551

    Name: EBI-306940

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: 14-3-3 protein eta

    Participant: 14-3-3 protein eta

    Accession: EBI-25395551

    Name: EBI-306940

    Confidences:

    Type
    Value
    No records found.

    19
    EBI-1387467
     logo
    PROTOR1
    Protein observed with Rictor-1
    PP610

    [+1]
    Proline-rich protein 5
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Proline-rich protein 5

    Participant: Proline-rich protein 5

    Accession: EBI-25395554

    Name: EBI-1387467

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 15.28

    P
    Participant: Proline-rich protein 5

    Participant: Proline-rich protein 5

    Accession: EBI-25395554

    Name: EBI-1387467

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Proline-rich protein 5

    Participant: Proline-rich protein 5

    Accession: EBI-25395554

    Name: EBI-1387467

    Stoichiometry: 0.0

    F
    Participant: Proline-rich protein 5

    Participant: Proline-rich protein 5

    Accession: EBI-25395554

    Name: EBI-1387467

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Proline-rich protein 5

    Participant: Proline-rich protein 5

    Accession: EBI-25395554

    Name: EBI-1387467

    Confidences:

    Type
    Value
    No records found.

    20
    EBI-347088
     logo
    YWHAZ
    Protein kinase C inhibitor protein 1
    14-3-3 protein zeta/delta
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: 14-3-3 protein zeta/delta

    Participant: 14-3-3 protein zeta/delta

    Accession: EBI-25395557

    Name: EBI-347088

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 6.49

    P
    Participant: 14-3-3 protein zeta/delta

    Participant: 14-3-3 protein zeta/delta

    Accession: EBI-25395557

    Name: EBI-347088

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: 14-3-3 protein zeta/delta

    Participant: 14-3-3 protein zeta/delta

    Accession: EBI-25395557

    Name: EBI-347088

    Stoichiometry: 0.0

    F
    Participant: 14-3-3 protein zeta/delta

    Participant: 14-3-3 protein zeta/delta

    Accession: EBI-25395557

    Name: EBI-347088

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: 14-3-3 protein zeta/delta

    Participant: 14-3-3 protein zeta/delta

    Accession: EBI-25395557

    Name: EBI-347088

    Confidences:

    Type
    Value
    No records found.


    Graphical Representation of Experimental Features