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What is this view?

Binary interactions

In this tab, we display the list of interactions that you have selected using one of our search features. Despite the fact that our data are annotated to accurately reflect the interactions reported in scientific literature, the data is shown in this view as binary interactions. Whenever the data was reported as a co-complex involving more than two molecules, we store it as such in the IntAct database and post-process it so the portal can show it as binary interaction. This post-processing is the Spoke Expansion model (connects bait to all preys):



sourceExp

At any moment you can choose to display the expansion column in this view in order to see which interaction are spoke expanded and which are not.

Description of what has changed

  • We have added more download options to allow users to retrieve their interaction set using more standard formats such as PSI-MI XML and PSIMITAB (version 2.5, 2.6 or 2.7) but also XGMML, RDF and Biopax (level 2 and 3).
  • We have now four different table views : minimal(molecule names and interaction AC), basic (minimal + molecule links, interaction detection method, negative), standard(minimal + molecule species, confidences, publication details, experiment details), expanded (standard + more experiment details) and complete (all mitab 2.7 columns).

Configuring the view to your need"

In the header of the interaction table you will find a button: ‘Change Column Display’ that will show you all the columns/Table views available and allow you to update the current selected set.

Downloading the data into Standard formats"

In the header of the interaction table you will find a drop down list that contains all the formats currently supported when downloading the interaction data. Select one of them and click the export button next to the list. Please note that PSI-MI XML is only available when the interaction set is no bigger than 1000 interactions.

Opening the interaction details"

Clicking on the magnifying glass in the first column of the interaction table will open the details of the corresponding interaction in the Interaction Details tab, giving you access to more details of the manually curated record.

What is this view?

Browsing (Browse Tab)

This tab is meant to give you access to more content based on the currently selected set of interactions. Please note that linking to third party resources will only include up to 200 molecules , if you exceed this number you will see the warning icon (This number has been reduced to 125 molecules for mRNA expression). Now let’s look at the features available to you:

Limiting the scope of the current dataset with the Uniprot Taxonomy ontology

Allows users to browse the Uniprot Taxonomy hierarchy as a tree and select terms in order to narrow down their dataset. Once a term is selected, you are taken back to the interaction tab to review your dataset.

Limiting the scope of the current dataset with the GO ontology

Allows users to browse the GO hierarchy as a tree and select terms in order to narrow down their dataset. Once a term is selected, you are taken back to the interaction tab to review your dataset.

Limiting the scope of the current dataset with the ChEBI ontology

Allows users to browse the ChEBI hierarchy as a tree and select terms in order to narrow down their dataset. Once a term is selected, you are taken back to the interaction tab to review your dataset.

Bulk linking to third party resources by using involved proteins

  • Proteins by Reactome pathway: Sends your proteins to the Reactome SkyPainter that will show you the pathways in which these molecules are know to play a role.
  • Proteins by Chromosomal location: Sends your list of proteins to Ensembl’s Karyotype viewer and overlays the proteins on the chromosomes.
  • Proteins by mRNA expression: Sends your set of proteins to the ArrayExpress Atlas that will show the known gene expression based on experimental studies.
What is this view?

Searching Interactions (Search Tab)

As you can see in this tab we are now trying to give you more targeted choice to do your queries, please note that the examples provided in this tab are live links so you can simply click them to see the resulting interactions sets.

Using the Quick Search

In this search panel you are free to type anything that might relate to interactions, whether it is properties of their interactor (gene name, identifiers, GO term…) or more specific to the interaction like publication, authors, experimental detection method, ...

Some examples:

  • Try the query: imatinib
    This is a drug for which we have curated a number of interactions.
    Once you press the search button you should be taken to the Interaction Tab that lists 130 binary interactions.
    If you want to construct more complex queries we recommend you take a look at the Molecular Interaction Query Language, accessible from the quick search panel.
  • Try the query: species:yeast AND type:"direct interaction"
    This query selects all interactions involving yeast interactors that have been shown to have direct interactions. If you customize the column display of the interaction tab, you will see that not only “direct interaction” have been selected but also children terms in the PSI-MI ontology.

Using the Ontology Search

Open the Rearch Tab. This panel is specialised to give you an easy access to ontology search. So far you can search on 4 ontologies:

  • Gene Ontology
  • InterPro
  • PSI-MI
  • ChEBI

Whenever you start typing a query in this search panel, the system will search as you type and propose a list of matching controlled vocabulary terms. You can then select one of them and select matching interactions.

For example, type: cancer
You will be presented with a few choices, please note that each term is followed by the count of matching interactions in the IntAct database.

Select a term with the mouse or using the keyboard cursor keys and you will be taken to the interaction tab.

Searching the Compound chemical structure

In this panel you will be able to draw all or part of a chemical structure and search for chemical compounds. If you get any matched, you can then see all interactions involving them.

First you have to open up the chemical search panel so that the applet can load, it might take a few seconds. Then you can start drawing your structure, for instance:

Once you have drawn your structure, select Similarity and press Search. You should be presented with a list of matching compound. Now choose one molecule and click the link: IntAct interactions. You will be taken to the interaction tab to review the data.

Complex Expansion

Binary interactions generated by co-complex expansion

Why should you care about complex expansion ?

Some experimental methods such as Tandem Affinity Purification do generate molecular interactions that can involve more than 2 molecules. Despite the fact that IntAct curation team do capture the molecular interaction as they were reported in the corresponding experiment, when you search using the intact web site, the results of your query is always shown as set of binary interactions (i.e. 2 molecules). We would like to draw your attention on the fact that whenever the reported interaction was a co-complex we do apply an expansion algorithm that transform this n-ary interaction into a set of binary interactions. While none of these agorithms is perfect and will very likely generate some false positive interactions, it is useful to present the data in a consistent manner. Bear in mind that we will strive to differentiate in the search results which interactions are a real experimental binary from expanded ones.

Existing expansion algorithm

There are several known algorithm allowing to transform an n-ary interaction into a set of binaries. The illustration below present the two well known expansion model and illustrates why they can be incorrect.



sourceExp

  • Spoke expansion: Links the bait molecule to all prey molecules. If N is the count of molecule in the complex, it generated N-1 binary interactions.
  • Matrix expansion: Links all molecule to all other molecule present in the complex. If N is the count of molecule in the complex, it generated (N*(N-1))/2 binary interactions.

Now the issue (as illustrated at the bottom right of the diagram above) with these two models lies in the fact that the real complex might not be articulated around the experimental bait but instead, this bait might be linked to a smaller complex, hence most binary interaction generated by spoke and matrix expansion result in false positive.



PSICQUIC

How is the number of interactions in other databases obtained?

PSICQUIC is a standard way to access molecular interaction databases across which it repeats the same query. The number of databases providing data may vary, depending on the status of their services and only those that are active are used in this query. By clicking on the number of interactions you will be redirected to the PSICQUIC View, where you can browse the results in those other resources.

The services currently active are:

Check the PSICQUIC site for more information.

IMEx

What is the significance of the IMEx dataset?"

IMEx is a network of databases which have agreed to supply a non-redundant set of data expertly manually annotated to the same consistent detailed standard which, as such, represents a high-quality subset of the data each individually provides. The number of databases providing data may vary, depending on the status of their services and only those that are active are used in this query. By clicking on the number of interactions you will be redirected to the IMEx View, where you can browse the results in those other resources.

The services currently active are:

Check the IMEx site for more information.

What is this view?

Representation of Experimental Features

This section shows the graphical representation of experimental features, where each participant is represented as a white rectangle with a black border and a line for each hundredth amino acid. All available features are attached to their associated participant and their categories are represented in the right side of the legend. The left side of the legend dynamically shows the range statuses occuring in the shown interaction. These are the possible range statuses:

sourceExp

Interacting with the widget

Hover over a feature to see more information in a tooltip.
sourceExp

To display a single interacting region click on it and click again to display all interacting regions.
Displaying all interacting regionsDisplaying one interacting region
sourceExpsourceExp
What is this view?

Dynamic molecular interaction data

This section shows the graphical representation of dynamic molecular interactions. By default it displays all the interactions from one experiment using radio buttons to allow users to highlight interactions in different variable conditions.

Publication

PubMed Id: 31980649

Journal: Nature Communications

Year of Publication: 2019

Title: Extensive Rewiring of the EGFR Network in Colorectal Cancer Cells Expressing Transforming Levels of KRASG13D

Author List: Kennedy SA, Jarboui MA, Srihari S, Raso C, Bryan K, Dernayka L, Charitou T, Bernal-Llinares M, Herrera-Montavez C, Krstic A, Matallanas D, Kotlyar M, Jurisica I, Curak J, Wong V, Stagljar I, LeBihan T, Imrie L, Pillai P, Lynn MA, Fasterius E, Al-Khalili Szigyarto C, Breen J, Kiel C, Serrano L, Rauch N, Rukhlenko O, Kholodenko BN, Iglesias-Martinez LF, Ryan CJ, Pilkington R, Cammareri P, Sansom O, Shave S, Auer M, Horn N, Klose F, Ueffing M, Boldt K, Lynn DJ, Kolch W

Cross References:

Annotations:

Topic
Text
Cancer - Interactions investigated in the context of cancer

Experiment (91 interactions)

Accession: EBI-25385328

Name: kennedy-2019-3

Host organism: human HKE-3 cell line

Organism: human HKE-3 cell line

Accession: EBI-22274219

Name: human-hke3

Description: human HKE-3 cell line

Interaction Detection Method: anti tag coip

Interaction detection method: anti tag coip
Accession: EBI-90

Name: anti tag coip

Description: anti tag coimmunoprecipitation

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0007
-
identity
pubmed
7708014
-
primary-reference


Annotations:
Topic
Text
definition
A specific antibody for the molecule of interest is not available, therefore the bait protein is expressed as a hybrid protein fused to a tag peptide/protein for which efficient and specific antibodies or a specific ligand are available.


Participant Identification Method: sequence tag

Participant identification method: sequence tag
Accession: EBI-81

Name: sequence tag

Description: sequence tag identification

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0102
-
identity
pubmed
10967324
-
primary-reference
pubmed
11752590
-
primary-reference
pubmed
11805837
-
primary-reference


Annotations:
Topic
Text
definition
This approach leads to protein identification by combining mass measurement and short amino acid sequence information obtained by tandem mass spectrometry. This information is then used to automatically find the best match in a sequence database. A mixture of peptides derived from a protease digestion is analysed by nanoelectrospray LC-MS/MS (Liquid Chromatography Tandem Mass Spectrometer or nanoESI MS/MS) mass spectrometry. Electrospray mass spectrometry cannot be applied to dilute samples and is affected by high salt. As a consequence peptides, normally extracted from acrylamide gels by in situ proteolysis, are desalted and concentrated on a microcolumn followed by elution into a capillary used for nanoelectrospray tandem mass spectrometry. A first mass spectrum (Normal mass spectrum or Q1 mass spectrum) gives information about the masses of all the peptides. Peptides observed in the normal mass spectrum are isolated in turn and dissociated into fragments by collision with gas molecules within the mass spectrometer. Some of the fragments obtained from a peptide constitute a nested set, differing by one amino acid, and the mass difference between them allows assignment of a partial sequence. The masses of the fragments define the position of the partial sequence in the peptide. Together with the cleavage specificity of the protease used to cleave the protein, and mass information such sequence tag provides much higher search specificity to match the a database entry. The procedure is repeated with several peptides from the digest, resulting in multiple identifications of the same protein or identification of several proteins from the peptide mixture. Unknown proteins can easily be identified by using the high specificity of the peptide sequence tag for searches in most sequence databases including EST or genome databases.


Cross References:

Annotations:

Topic
Text
The entry is part of the PrimesDB project, an IMEx observer member.
95 bait proteins were selected based on a highly curated EGFR signaling. The baits were expressed as FLAG-tagged proteins carefully titrating transfection to achieve a similar expression level in both cell lines (HCT116 and HKE3). Baits were immunoprecipitated and associated prey proteins were identified by high- resolution Orbitrap qMS. We defined preys as being “rewired” in EGFRNet mtKRAS-Lo (HKE3 cells), if the abundance of the prey protein in the pull-down was significantly different (P <= 0.05) compared to EGFRNet mtKRAS-Hi (HCT116 cells) and the significance A value for the prey protein was also <= 0.05. Interactions that were identified in only one EGFRNet, but where prey abundance was subsequently not found to be statistically significantly different in the respective bait-prey complexes in the two cell lines were not considered as rewired preys. (Only for 91 baits interactions were found).
imex curation
walter.kolch@ucd.ie,david.lynn@sahmri.com,karsten.boldt@uni-tuebingen.de
2019-12-05: Submitted by David J. Lynn, EMBL Australia Group, Adelaide, Australia

Interaction

Accession: EBI-25391779

Name: map2k2-araf-5

Description: -

Type: association

Interaction type: association
Accession: EBI-1813191

Name: association

Description: association

Cross References:
Database
Identifier
Secondary identifier
Qualifier
pubmed
14755292
-
primary-reference
psi-mi
MI:0914
-
identity


Annotations:
Topic
Text
definition
Interaction between molecules that may participate in formation of one, but possibly more, physical complexes. Often describes a set of molecules that are co-purified in a single pull-down or coimmunoprecipitation but might participate in formation of distinct physical complexes sharing a common bait.


Cross References:

Database
Identifier
Secondary identifier
Qualifier
-
-

Annotations:

Topic
Text
Suppl Data 2, 4 and 5

Participants (33)

Legend:
A
Annotation and Cross Reference  
P
Experimental Parameter   
S
Stoichiometry   
F
Experimental Feature
C
Participant Confidence
pp12pp
#
Name
Links
Primary Identifier
Aliases
Description
Species
Expression system
Experimental role
Biological role
Interactor type
More...
pp12pp
1
EBI-1056930
 logo
MAP2K2
MEK2
MKK2

[+3]
Dual specificity mitogen-activated protein kinase kinase 2
Homo sapiens
Organism Details

Accession: EBI-874

Name: human

Description: Homo sapiens

Cross References:

Database
Identifier
Secondary identifier
Qualifier
human

Expressed In Details

Accession:

Name:

-
bait
Experimental role: bait
Accession: EBI-49

Name: bait

Description: bait

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0496
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Molecule experimentally treated to capture its interacting partners.


unspecified role
Biological role: unspecified role
Accession: EBI-77781

Name: unspecified role

Description: unspecified role

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0499
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Role not specified or not applicable to the data.


protein
Interactor type: protein
Accession: EBI-619654

Name: protein

Description: protein

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0326
-
identity
pubmed
14755292
-
primary-reference
so
SO:0000358
-
see-also


Annotations:
Topic
Text
definition
A linear polymer of amino acids joined by peptide bonds in a specific sequence.


A
Participant: Dual specificity mitogen-activated protein kinase kinase 2

Participant: Dual specificity mitogen-activated protein kinase kinase 2

Accession: EBI-25391783

Name: EBI-1056930

P
Participant: Dual specificity mitogen-activated protein kinase kinase 2

Participant: Dual specificity mitogen-activated protein kinase kinase 2

Accession: EBI-25391783

Name: EBI-1056930

Parameters:

Type
Value
Unit
Base
Exponent
Uncertainty
No records found.


S
Participant: Dual specificity mitogen-activated protein kinase kinase 2

Participant: Dual specificity mitogen-activated protein kinase kinase 2

Accession: EBI-25391783

Name: EBI-1056930

Stoichiometry: 0.0

F
Participant: Dual specificity mitogen-activated protein kinase kinase 2

Participant: Dual specificity mitogen-activated protein kinase kinase 2

Accession: EBI-25391783

Name: EBI-1056930

Features:

Legend:
A
Annotation and Cross Reference  
Feature Name
Feature Type
Detection Method
Range positions
More...
flag tag
  • n-n
  • A
    Feature: EBI-25391785

    Feature: flag tag

    Accession: EBI-25391785

    Name: flag tag


    C
    Participant: Dual specificity mitogen-activated protein kinase kinase 2

    Participant: Dual specificity mitogen-activated protein kinase kinase 2

    Accession: EBI-25391783

    Name: EBI-1056930

    Confidences:

    Type
    Value
    No records found.

    2
    EBI-1103245
     logo
    TUBA1C
    TUBA6
    Tubulin alpha-6 chain

    [+1]
    Tubulin alpha-1C chain
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference
    so
    SO:0000358
    -
    see-also


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Tubulin alpha-1C chain

    Participant: Tubulin alpha-1C chain

    Accession: EBI-25391787

    Name: EBI-1103245

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 2.57

    P
    Participant: Tubulin alpha-1C chain

    Participant: Tubulin alpha-1C chain

    Accession: EBI-25391787

    Name: EBI-1103245

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Tubulin alpha-1C chain

    Participant: Tubulin alpha-1C chain

    Accession: EBI-25391787

    Name: EBI-1103245

    Stoichiometry: 0.0

    F
    Participant: Tubulin alpha-1C chain

    Participant: Tubulin alpha-1C chain

    Accession: EBI-25391787

    Name: EBI-1103245

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Tubulin alpha-1C chain

    Participant: Tubulin alpha-1C chain

    Accession: EBI-25391787

    Name: EBI-1103245

    Confidences:

    Type
    Value
    No records found.

    3
    EBI-486984
     logo
    KSR1
    KSR
    Kinase suppressor of Ras 1
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference
    so
    SO:0000358
    -
    see-also


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Kinase suppressor of Ras 1

    Participant: Kinase suppressor of Ras 1

    Accession: EBI-25391790

    Name: EBI-486984

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 4.46

    P
    Participant: Kinase suppressor of Ras 1

    Participant: Kinase suppressor of Ras 1

    Accession: EBI-25391790

    Name: EBI-486984

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Kinase suppressor of Ras 1

    Participant: Kinase suppressor of Ras 1

    Accession: EBI-25391790

    Name: EBI-486984

    Stoichiometry: 0.0

    F
    Participant: Kinase suppressor of Ras 1

    Participant: Kinase suppressor of Ras 1

    Accession: EBI-25391790

    Name: EBI-486984

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Kinase suppressor of Ras 1

    Participant: Kinase suppressor of Ras 1

    Accession: EBI-25391790

    Name: EBI-486984

    Confidences:

    Type
    Value
    No records found.

    4
    EBI-473801
     logo
    Mitofilin
    p87/89
    Cell proliferation-inducing gene 4/52 protein

    [+7]
    MICOS complex subunit MIC60
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference
    so
    SO:0000358
    -
    see-also


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: MICOS complex subunit MIC60

    Participant: MICOS complex subunit MIC60

    Accession: EBI-25391793

    Name: EBI-473801

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 4.11

    P
    Participant: MICOS complex subunit MIC60

    Participant: MICOS complex subunit MIC60

    Accession: EBI-25391793

    Name: EBI-473801

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: MICOS complex subunit MIC60

    Participant: MICOS complex subunit MIC60

    Accession: EBI-25391793

    Name: EBI-473801

    Stoichiometry: 0.0

    F
    Participant: MICOS complex subunit MIC60

    Participant: MICOS complex subunit MIC60

    Accession: EBI-25391793

    Name: EBI-473801

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: MICOS complex subunit MIC60

    Participant: MICOS complex subunit MIC60

    Accession: EBI-25391793

    Name: EBI-473801

    Confidences:

    Type
    Value
    No records found.

    5
    EBI-295634
     logo
    CDC37
    Hsp90 chaperone protein kinase-targeting subunit
    p50Cdc37

    [+1]
    Hsp90 co-chaperone Cdc37
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference
    so
    SO:0000358
    -
    see-also


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Hsp90 co-chaperone Cdc37

    Participant: Hsp90 co-chaperone Cdc37

    Accession: EBI-25391796

    Name: EBI-295634

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 9.24

    P
    Participant: Hsp90 co-chaperone Cdc37

    Participant: Hsp90 co-chaperone Cdc37

    Accession: EBI-25391796

    Name: EBI-295634

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Hsp90 co-chaperone Cdc37

    Participant: Hsp90 co-chaperone Cdc37

    Accession: EBI-25391796

    Name: EBI-295634

    Stoichiometry: 0.0

    F
    Participant: Hsp90 co-chaperone Cdc37

    Participant: Hsp90 co-chaperone Cdc37

    Accession: EBI-25391796

    Name: EBI-295634

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Hsp90 co-chaperone Cdc37

    Participant: Hsp90 co-chaperone Cdc37

    Accession: EBI-25391796

    Name: EBI-295634

    Confidences:

    Type
    Value
    No records found.

    6
    EBI-353193
     logo
    PRDX1
    PAGA
    PAGB

    [+6]
    Peroxiredoxin-1
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference
    so
    SO:0000358
    -
    see-also


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Peroxiredoxin-1

    Participant: Peroxiredoxin-1

    Accession: EBI-25391799

    Name: EBI-353193

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 1.67

    P
    Participant: Peroxiredoxin-1

    Participant: Peroxiredoxin-1

    Accession: EBI-25391799

    Name: EBI-353193

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Peroxiredoxin-1

    Participant: Peroxiredoxin-1

    Accession: EBI-25391799

    Name: EBI-353193

    Stoichiometry: 0.0

    F
    Participant: Peroxiredoxin-1

    Participant: Peroxiredoxin-1

    Accession: EBI-25391799

    Name: EBI-353193

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Peroxiredoxin-1

    Participant: Peroxiredoxin-1

    Accession: EBI-25391799

    Name: EBI-353193

    Confidences:

    Type
    Value
    No records found.

    7
    EBI-306940
     logo
    YWHAH
    YWHA1
    Protein AS1
    14-3-3 protein eta
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference
    so
    SO:0000358
    -
    see-also


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: 14-3-3 protein eta

    Participant: 14-3-3 protein eta

    Accession: EBI-25391802

    Name: EBI-306940

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 9.44

    P
    Participant: 14-3-3 protein eta

    Participant: 14-3-3 protein eta

    Accession: EBI-25391802

    Name: EBI-306940

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: 14-3-3 protein eta

    Participant: 14-3-3 protein eta

    Accession: EBI-25391802

    Name: EBI-306940

    Stoichiometry: 0.0

    F
    Participant: 14-3-3 protein eta

    Participant: 14-3-3 protein eta

    Accession: EBI-25391802

    Name: EBI-306940

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: 14-3-3 protein eta

    Participant: 14-3-3 protein eta

    Accession: EBI-25391802

    Name: EBI-306940

    Confidences:

    Type
    Value
    No records found.

    8
    EBI-359174
     logo
    SLC25A11
    SLC20A4
    Solute carrier family 25 member 11
    Mitochondrial 2-oxoglutarate/malate carrier protein
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference
    so
    SO:0000358
    -
    see-also


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Mitochondrial 2-oxoglutarate/malate carrier protein

    Participant: Mitochondrial 2-oxoglutarate/malate carrier protein

    Accession: EBI-25391805

    Name: EBI-359174

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 2.59

    P
    Participant: Mitochondrial 2-oxoglutarate/malate carrier protein

    Participant: Mitochondrial 2-oxoglutarate/malate carrier protein

    Accession: EBI-25391805

    Name: EBI-359174

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Mitochondrial 2-oxoglutarate/malate carrier protein

    Participant: Mitochondrial 2-oxoglutarate/malate carrier protein

    Accession: EBI-25391805

    Name: EBI-359174

    Stoichiometry: 0.0

    F
    Participant: Mitochondrial 2-oxoglutarate/malate carrier protein

    Participant: Mitochondrial 2-oxoglutarate/malate carrier protein

    Accession: EBI-25391805

    Name: EBI-359174

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Mitochondrial 2-oxoglutarate/malate carrier protein

    Participant: Mitochondrial 2-oxoglutarate/malate carrier protein

    Accession: EBI-25391805

    Name: EBI-359174

    Confidences:

    Type
    Value
    No records found.

    9
    EBI-1045757
     logo
    CDHF4
    Desmosomal glycoprotein 1
    Pemphigus foliaceus antigen

    [+2]
    Desmoglein-1
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference
    so
    SO:0000358
    -
    see-also


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Desmoglein-1

    Participant: Desmoglein-1

    Accession: EBI-25391808

    Name: EBI-1045757

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    -

    Annotations:

    Topic
    Text
    Prey abundance was significantly different between HCT116 and HKE3 cells.
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 4.06
    SILAC Ratio (Prey in HCT116 IP / Prey in HKE3 IP): 0.10

    P
    Participant: Desmoglein-1

    Participant: Desmoglein-1

    Accession: EBI-25391808

    Name: EBI-1045757

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Desmoglein-1

    Participant: Desmoglein-1

    Accession: EBI-25391808

    Name: EBI-1045757

    Stoichiometry: 0.0

    F
    Participant: Desmoglein-1

    Participant: Desmoglein-1

    Accession: EBI-25391808

    Name: EBI-1045757

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Desmoglein-1

    Participant: Desmoglein-1

    Accession: EBI-25391808

    Name: EBI-1045757

    Confidences:

    Type
    Value
    No records found.

    10
    EBI-358549
     logo
    SLC25A3
    PHC
    OK/SW-cl.48

    [+2]
    Phosphate carrier protein, mitochondrial
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference
    so
    SO:0000358
    -
    see-also


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Phosphate carrier protein, mitochondrial

    Participant: Phosphate carrier protein, mitochondrial

    Accession: EBI-25391814

    Name: EBI-358549

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 1.62

    P
    Participant: Phosphate carrier protein, mitochondrial

    Participant: Phosphate carrier protein, mitochondrial

    Accession: EBI-25391814

    Name: EBI-358549

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Phosphate carrier protein, mitochondrial

    Participant: Phosphate carrier protein, mitochondrial

    Accession: EBI-25391814

    Name: EBI-358549

    Stoichiometry: 0.0

    F
    Participant: Phosphate carrier protein, mitochondrial

    Participant: Phosphate carrier protein, mitochondrial

    Accession: EBI-25391814

    Name: EBI-358549

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Phosphate carrier protein, mitochondrial

    Participant: Phosphate carrier protein, mitochondrial

    Accession: EBI-25391814

    Name: EBI-358549

    Confidences:

    Type
    Value
    No records found.

    11
    EBI-351356
     logo
    TUBB4B
    TUBB2C
    Tubulin beta-2 chain

    [+1]
    Tubulin beta-4B chain
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference
    so
    SO:0000358
    -
    see-also


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Tubulin beta-4B chain

    Participant: Tubulin beta-4B chain

    Accession: EBI-25391817

    Name: EBI-351356

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 2.18

    P
    Participant: Tubulin beta-4B chain

    Participant: Tubulin beta-4B chain

    Accession: EBI-25391817

    Name: EBI-351356

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Tubulin beta-4B chain

    Participant: Tubulin beta-4B chain

    Accession: EBI-25391817

    Name: EBI-351356

    Stoichiometry: 0.0

    F
    Participant: Tubulin beta-4B chain

    Participant: Tubulin beta-4B chain

    Accession: EBI-25391817

    Name: EBI-351356

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Tubulin beta-4B chain

    Participant: Tubulin beta-4B chain

    Accession: EBI-25391817

    Name: EBI-351356

    Confidences:

    Type
    Value
    No records found.

    12
    EBI-351772
     logo
    TUBA4A
    Tubulin alpha-1 chain
    Alpha-tubulin 1

    [+3]
    Tubulin alpha-4A chain
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference
    so
    SO:0000358
    -
    see-also


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Tubulin alpha-4A chain

    Participant: Tubulin alpha-4A chain

    Accession: EBI-25391820

    Name: EBI-351772

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 2.45

    P
    Participant: Tubulin alpha-4A chain

    Participant: Tubulin alpha-4A chain

    Accession: EBI-25391820

    Name: EBI-351772

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Tubulin alpha-4A chain

    Participant: Tubulin alpha-4A chain

    Accession: EBI-25391820

    Name: EBI-351772

    Stoichiometry: 0.0

    F
    Participant: Tubulin alpha-4A chain

    Participant: Tubulin alpha-4A chain

    Accession: EBI-25391820

    Name: EBI-351772

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Tubulin alpha-4A chain

    Participant: Tubulin alpha-4A chain

    Accession: EBI-25391820

    Name: EBI-351772

    Confidences:

    Type
    Value
    No records found.

    13
    EBI-487083
     logo
    TUBA1B
    Tubulin alpha-ubiquitous chain
    Alpha-tubulin ubiquitous

    [+1]
    Tubulin alpha-1B chain
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference
    so
    SO:0000358
    -
    see-also


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Tubulin alpha-1B chain

    Participant: Tubulin alpha-1B chain

    Accession: EBI-25391823

    Name: EBI-487083

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 2.45

    P
    Participant: Tubulin alpha-1B chain

    Participant: Tubulin alpha-1B chain

    Accession: EBI-25391823

    Name: EBI-487083

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Tubulin alpha-1B chain

    Participant: Tubulin alpha-1B chain

    Accession: EBI-25391823

    Name: EBI-487083

    Stoichiometry: 0.0

    F
    Participant: Tubulin alpha-1B chain

    Participant: Tubulin alpha-1B chain

    Accession: EBI-25391823

    Name: EBI-487083

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Tubulin alpha-1B chain

    Participant: Tubulin alpha-1B chain

    Accession: EBI-25391823

    Name: EBI-487083

    Confidences:

    Type
    Value
    No records found.

    14
    EBI-347088
     logo
    YWHAZ
    Protein kinase C inhibitor protein 1
    14-3-3 protein zeta/delta
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference
    so
    SO:0000358
    -
    see-also


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: 14-3-3 protein zeta/delta

    Participant: 14-3-3 protein zeta/delta

    Accession: EBI-25391826

    Name: EBI-347088

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    -

    Annotations:

    Topic
    Text
    Prey abundance was significantly different between HCT116 and HKE3 cells.
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 9.68
    SILAC Ratio (Prey in HCT116 IP / Prey in HKE3 IP): 0.39

    P
    Participant: 14-3-3 protein zeta/delta

    Participant: 14-3-3 protein zeta/delta

    Accession: EBI-25391826

    Name: EBI-347088

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: 14-3-3 protein zeta/delta

    Participant: 14-3-3 protein zeta/delta

    Accession: EBI-25391826

    Name: EBI-347088

    Stoichiometry: 0.0

    F
    Participant: 14-3-3 protein zeta/delta

    Participant: 14-3-3 protein zeta/delta

    Accession: EBI-25391826

    Name: EBI-347088

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: 14-3-3 protein zeta/delta

    Participant: 14-3-3 protein zeta/delta

    Accession: EBI-25391826

    Name: EBI-347088

    Confidences:

    Type
    Value
    No records found.

    15
    EBI-356687
     logo
    CCT6A
    CCT6
    CCTZ

    [+4]
    T-complex protein 1 subunit zeta
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference
    so
    SO:0000358
    -
    see-also


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: T-complex protein 1 subunit zeta

    Participant: T-complex protein 1 subunit zeta

    Accession: EBI-25391832

    Name: EBI-356687

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 1.60

    P
    Participant: T-complex protein 1 subunit zeta

    Participant: T-complex protein 1 subunit zeta

    Accession: EBI-25391832

    Name: EBI-356687

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: T-complex protein 1 subunit zeta

    Participant: T-complex protein 1 subunit zeta

    Accession: EBI-25391832

    Name: EBI-356687

    Stoichiometry: 0.0

    F
    Participant: T-complex protein 1 subunit zeta

    Participant: T-complex protein 1 subunit zeta

    Accession: EBI-25391832

    Name: EBI-356687

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: T-complex protein 1 subunit zeta

    Participant: T-complex protein 1 subunit zeta

    Accession: EBI-25391832

    Name: EBI-356687

    Confidences:

    Type
    Value
    No records found.

    16
    EBI-354932
     logo
    HSPA9
    GRP75
    HSPA9B

    [+5]
    Stress-70 protein, mitochondrial
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference
    so
    SO:0000358
    -
    see-also


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Stress-70 protein, mitochondrial

    Participant: Stress-70 protein, mitochondrial

    Accession: EBI-25391835

    Name: EBI-354932

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 3.06

    P
    Participant: Stress-70 protein, mitochondrial

    Participant: Stress-70 protein, mitochondrial

    Accession: EBI-25391835

    Name: EBI-354932

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Stress-70 protein, mitochondrial

    Participant: Stress-70 protein, mitochondrial

    Accession: EBI-25391835

    Name: EBI-354932

    Stoichiometry: 0.0

    F
    Participant: Stress-70 protein, mitochondrial

    Participant: Stress-70 protein, mitochondrial

    Accession: EBI-25391835

    Name: EBI-354932

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Stress-70 protein, mitochondrial

    Participant: Stress-70 protein, mitochondrial

    Accession: EBI-25391835

    Name: EBI-354932

    Confidences:

    Type
    Value
    No records found.

    17
    EBI-356933
     logo
    HSPA4
    APG2
    Heat shock 70-related protein APG-2

    [+1]
    Heat shock 70 kDa protein 4
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference
    so
    SO:0000358
    -
    see-also


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Heat shock 70 kDa protein 4

    Participant: Heat shock 70 kDa protein 4

    Accession: EBI-25391838

    Name: EBI-356933

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 3.25

    P
    Participant: Heat shock 70 kDa protein 4

    Participant: Heat shock 70 kDa protein 4

    Accession: EBI-25391838

    Name: EBI-356933

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Heat shock 70 kDa protein 4

    Participant: Heat shock 70 kDa protein 4

    Accession: EBI-25391838

    Name: EBI-356933

    Stoichiometry: 0.0

    F
    Participant: Heat shock 70 kDa protein 4

    Participant: Heat shock 70 kDa protein 4

    Accession: EBI-25391838

    Name: EBI-356933

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Heat shock 70 kDa protein 4

    Participant: Heat shock 70 kDa protein 4

    Accession: EBI-25391838

    Name: EBI-356933

    Confidences:

    Type
    Value
    No records found.

    18
    EBI-359815
     logo
    YWHAB
    Protein kinase C inhibitor protein 1
    Protein 1054
    14-3-3 protein beta/alpha
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference
    so
    SO:0000358
    -
    see-also


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: 14-3-3 protein beta/alpha

    Participant: 14-3-3 protein beta/alpha

    Accession: EBI-25391841

    Name: EBI-359815

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    -

    Annotations:

    Topic
    Text
    Prey abundance was significantly different between HCT116 and HKE3 cells.
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 8.96
    SILAC Ratio (Prey in HCT116 IP / Prey in HKE3 IP): 0.44

    P
    Participant: 14-3-3 protein beta/alpha

    Participant: 14-3-3 protein beta/alpha

    Accession: EBI-25391841

    Name: EBI-359815

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: 14-3-3 protein beta/alpha

    Participant: 14-3-3 protein beta/alpha

    Accession: EBI-25391841

    Name: EBI-359815

    Stoichiometry: 0.0

    F
    Participant: 14-3-3 protein beta/alpha

    Participant: 14-3-3 protein beta/alpha

    Accession: EBI-25391841

    Name: EBI-359815

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: 14-3-3 protein beta/alpha

    Participant: 14-3-3 protein beta/alpha

    Accession: EBI-25391841

    Name: EBI-359815

    Confidences:

    Type
    Value
    No records found.

    19
    EBI-347834
     logo
    DNAJA1
    DNAJ2
    HDJ2

    [+7]
    DnaJ homolog subfamily A member 1
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference
    so
    SO:0000358
    -
    see-also


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: DnaJ homolog subfamily A member 1

    Participant: DnaJ homolog subfamily A member 1

    Accession: EBI-25391847

    Name: EBI-347834

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 1.74

    P
    Participant: DnaJ homolog subfamily A member 1

    Participant: DnaJ homolog subfamily A member 1

    Accession: EBI-25391847

    Name: EBI-347834

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: DnaJ homolog subfamily A member 1

    Participant: DnaJ homolog subfamily A member 1

    Accession: EBI-25391847

    Name: EBI-347834

    Stoichiometry: 0.0

    F
    Participant: DnaJ homolog subfamily A member 1

    Participant: DnaJ homolog subfamily A member 1

    Accession: EBI-25391847

    Name: EBI-347834

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: DnaJ homolog subfamily A member 1

    Participant: DnaJ homolog subfamily A member 1

    Accession: EBI-25391847

    Name: EBI-347834

    Confidences:

    Type
    Value
    No records found.

    20
    EBI-359854
     logo
    YWHAQ
    14-3-3 protein tau
    14-3-3 protein T-cell

    [+1]
    14-3-3 protein theta
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference
    so
    SO:0000358
    -
    see-also


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: 14-3-3 protein theta

    Participant: 14-3-3 protein theta

    Accession: EBI-25391850

    Name: EBI-359854

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    -

    Annotations:

    Topic
    Text
    Prey abundance was significantly different between HCT116 and HKE3 cells.
    SILAC Ratio (Prey in HKE3 IP / Prey in control): 9.49
    SILAC Ratio (Prey in HCT116 IP / Prey in HKE3 IP): 0.34

    P
    Participant: 14-3-3 protein theta

    Participant: 14-3-3 protein theta

    Accession: EBI-25391850

    Name: EBI-359854

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: 14-3-3 protein theta

    Participant: 14-3-3 protein theta

    Accession: EBI-25391850

    Name: EBI-359854

    Stoichiometry: 0.0

    F
    Participant: 14-3-3 protein theta

    Participant: 14-3-3 protein theta

    Accession: EBI-25391850

    Name: EBI-359854

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: 14-3-3 protein theta

    Participant: 14-3-3 protein theta

    Accession: EBI-25391850

    Name: EBI-359854

    Confidences:

    Type
    Value
    No records found.


    Graphical Representation of Experimental Features