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What is this view?

Binary interactions

In this tab, we display the list of interactions that you have selected using one of our search features. Despite the fact that our data are annotated to accurately reflect the interactions reported in scientific literature, the data is shown in this view as binary interactions. Whenever the data was reported as a co-complex involving more than two molecules, we store it as such in the IntAct database and post-process it so the portal can show it as binary interaction. This post-processing is the Spoke Expansion model (connects bait to all preys):



sourceExp

At any moment you can choose to display the expansion column in this view in order to see which interaction are spoke expanded and which are not.

Description of what has changed

  • We have added more download options to allow users to retrieve their interaction set using more standard formats such as PSI-MI XML and PSIMITAB (version 2.5, 2.6 or 2.7) but also XGMML, RDF and Biopax (level 2 and 3).
  • We have now four different table views : minimal(molecule names and interaction AC), basic (minimal + molecule links, interaction detection method, negative), standard(minimal + molecule species, confidences, publication details, experiment details), expanded (standard + more experiment details) and complete (all mitab 2.7 columns).

Configuring the view to your need"

In the header of the interaction table you will find a button: ‘Change Column Display’ that will show you all the columns/Table views available and allow you to update the current selected set.

Downloading the data into Standard formats"

In the header of the interaction table you will find a drop down list that contains all the formats currently supported when downloading the interaction data. Select one of them and click the export button next to the list. Please note that PSI-MI XML is only available when the interaction set is no bigger than 1000 interactions.

Opening the interaction details"

Clicking on the magnifying glass in the first column of the interaction table will open the details of the corresponding interaction in the Interaction Details tab, giving you access to more details of the manually curated record.

What is this view?

Browsing (Browse Tab)

This tab is meant to give you access to more content based on the currently selected set of interactions. Please note that linking to third party resources will only include up to 200 molecules , if you exceed this number you will see the warning icon (This number has been reduced to 125 molecules for mRNA expression). Now let’s look at the features available to you:

Limiting the scope of the current dataset with the Uniprot Taxonomy ontology

Allows users to browse the Uniprot Taxonomy hierarchy as a tree and select terms in order to narrow down their dataset. Once a term is selected, you are taken back to the interaction tab to review your dataset.

Limiting the scope of the current dataset with the GO ontology

Allows users to browse the GO hierarchy as a tree and select terms in order to narrow down their dataset. Once a term is selected, you are taken back to the interaction tab to review your dataset.

Limiting the scope of the current dataset with the ChEBI ontology

Allows users to browse the ChEBI hierarchy as a tree and select terms in order to narrow down their dataset. Once a term is selected, you are taken back to the interaction tab to review your dataset.

Bulk linking to third party resources by using involved proteins

  • Proteins by Reactome pathway: Sends your proteins to the Reactome SkyPainter that will show you the pathways in which these molecules are know to play a role.
  • Proteins by Chromosomal location: Sends your list of proteins to Ensembl’s Karyotype viewer and overlays the proteins on the chromosomes.
  • Proteins by mRNA expression: Sends your set of proteins to the ArrayExpress Atlas that will show the known gene expression based on experimental studies.
What is this view?

Searching Interactions (Search Tab)

As you can see in this tab we are now trying to give you more targeted choice to do your queries, please note that the examples provided in this tab are live links so you can simply click them to see the resulting interactions sets.

Using the Quick Search

In this search panel you are free to type anything that might relate to interactions, whether it is properties of their interactor (gene name, identifiers, GO term…) or more specific to the interaction like publication, authors, experimental detection method, ...

Some examples:

  • Try the query: imatinib
    This is a drug for which we have curated a number of interactions.
    Once you press the search button you should be taken to the Interaction Tab that lists 130 binary interactions.
    If you want to construct more complex queries we recommend you take a look at the Molecular Interaction Query Language, accessible from the quick search panel.
  • Try the query: species:yeast AND type:"direct interaction"
    This query selects all interactions involving yeast interactors that have been shown to have direct interactions. If you customize the column display of the interaction tab, you will see that not only “direct interaction” have been selected but also children terms in the PSI-MI ontology.

Using the Ontology Search

Open the Rearch Tab. This panel is specialised to give you an easy access to ontology search. So far you can search on 4 ontologies:

  • Gene Ontology
  • InterPro
  • PSI-MI
  • ChEBI

Whenever you start typing a query in this search panel, the system will search as you type and propose a list of matching controlled vocabulary terms. You can then select one of them and select matching interactions.

For example, type: cancer
You will be presented with a few choices, please note that each term is followed by the count of matching interactions in the IntAct database.

Select a term with the mouse or using the keyboard cursor keys and you will be taken to the interaction tab.

Searching the Compound chemical structure

In this panel you will be able to draw all or part of a chemical structure and search for chemical compounds. If you get any matched, you can then see all interactions involving them.

First you have to open up the chemical search panel so that the applet can load, it might take a few seconds. Then you can start drawing your structure, for instance:

Once you have drawn your structure, select Similarity and press Search. You should be presented with a list of matching compound. Now choose one molecule and click the link: IntAct interactions. You will be taken to the interaction tab to review the data.

Complex Expansion

Binary interactions generated by co-complex expansion

Why should you care about complex expansion ?

Some experimental methods such as Tandem Affinity Purification do generate molecular interactions that can involve more than 2 molecules. Despite the fact that IntAct curation team do capture the molecular interaction as they were reported in the corresponding experiment, when you search using the intact web site, the results of your query is always shown as set of binary interactions (i.e. 2 molecules). We would like to draw your attention on the fact that whenever the reported interaction was a co-complex we do apply an expansion algorithm that transform this n-ary interaction into a set of binary interactions. While none of these agorithms is perfect and will very likely generate some false positive interactions, it is useful to present the data in a consistent manner. Bear in mind that we will strive to differentiate in the search results which interactions are a real experimental binary from expanded ones.

Existing expansion algorithm

There are several known algorithm allowing to transform an n-ary interaction into a set of binaries. The illustration below present the two well known expansion model and illustrates why they can be incorrect.



sourceExp

  • Spoke expansion: Links the bait molecule to all prey molecules. If N is the count of molecule in the complex, it generated N-1 binary interactions.
  • Matrix expansion: Links all molecule to all other molecule present in the complex. If N is the count of molecule in the complex, it generated (N*(N-1))/2 binary interactions.

Now the issue (as illustrated at the bottom right of the diagram above) with these two models lies in the fact that the real complex might not be articulated around the experimental bait but instead, this bait might be linked to a smaller complex, hence most binary interaction generated by spoke and matrix expansion result in false positive.



PSICQUIC

How is the number of interactions in other databases obtained?

PSICQUIC is a standard way to access molecular interaction databases across which it repeats the same query. The number of databases providing data may vary, depending on the status of their services and only those that are active are used in this query. By clicking on the number of interactions you will be redirected to the PSICQUIC View, where you can browse the results in those other resources.

The services currently active are:

Check the PSICQUIC site for more information.

IMEx

What is the significance of the IMEx dataset?"

IMEx is a network of databases which have agreed to supply a non-redundant set of data expertly manually annotated to the same consistent detailed standard which, as such, represents a high-quality subset of the data each individually provides. The number of databases providing data may vary, depending on the status of their services and only those that are active are used in this query. By clicking on the number of interactions you will be redirected to the IMEx View, where you can browse the results in those other resources.

The services currently active are:

Check the IMEx site for more information.

What is this view?

Representation of Experimental Features

This section shows the graphical representation of experimental features, where each participant is represented as a white rectangle with a black border and a line for each hundredth amino acid. All available features are attached to their associated participant and their categories are represented in the right side of the legend. The left side of the legend dynamically shows the range statuses occuring in the shown interaction. These are the possible range statuses:

sourceExp

Interacting with the widget

Hover over a feature to see more information in a tooltip.
sourceExp

To display a single interacting region click on it and click again to display all interacting regions.
Displaying all interacting regionsDisplaying one interacting region
sourceExpsourceExp
What is this view?

Dynamic molecular interaction data

This section shows the graphical representation of dynamic molecular interactions. By default it displays all the interactions from one experiment using radio buttons to allow users to highlight interactions in different variable conditions.

Publication

PubMed Id: 31980649

Journal: Nature Communications

Year of Publication: 2019

Title: Extensive Rewiring of the EGFR Network in Colorectal Cancer Cells Expressing Transforming Levels of KRASG13D

Author List: Kennedy SA, Jarboui MA, Srihari S, Raso C, Bryan K, Dernayka L, Charitou T, Bernal-Llinares M, Herrera-Montavez C, Krstic A, Matallanas D, Kotlyar M, Jurisica I, Curak J, Wong V, Stagljar I, LeBihan T, Imrie L, Pillai P, Lynn MA, Fasterius E, Al-Khalili Szigyarto C, Breen J, Kiel C, Serrano L, Rauch N, Rukhlenko O, Kholodenko BN, Iglesias-Martinez LF, Ryan CJ, Pilkington R, Cammareri P, Sansom O, Shave S, Auer M, Horn N, Klose F, Ueffing M, Boldt K, Lynn DJ, Kolch W

Cross References:

Annotations:

Topic
Text
Cancer - Interactions investigated in the context of cancer

Experiment (93 interactions)

Accession: EBI-25372720

Name: kennedy-2019-2

Host organism: Human colon carcinoma HCT116 cells

Organism: Human colon carcinoma HCT116 cells

Accession: EBI-727882

Name: human-hct116

Description: Human colon carcinoma HCT116 cells

Cross References:

Database
Identifier
Secondary identifier
Qualifier
human

Interaction Detection Method: anti tag coip

Interaction detection method: anti tag coip
Accession: EBI-90

Name: anti tag coip

Description: anti tag coimmunoprecipitation

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0007
-
identity
pubmed
7708014
-
primary-reference


Annotations:
Topic
Text
definition
A specific antibody for the molecule of interest is not available, therefore the bait protein is expressed as a hybrid protein fused to a tag peptide/protein for which efficient and specific antibodies or a specific ligand are available.


Participant Identification Method: sequence tag

Participant identification method: sequence tag
Accession: EBI-81

Name: sequence tag

Description: sequence tag identification

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0102
-
identity
pubmed
10967324
-
primary-reference
pubmed
11752590
-
primary-reference
pubmed
11805837
-
primary-reference


Annotations:
Topic
Text
definition
This approach leads to protein identification by combining mass measurement and short amino acid sequence information obtained by tandem mass spectrometry. This information is then used to automatically find the best match in a sequence database. A mixture of peptides derived from a protease digestion is analysed by nanoelectrospray LC-MS/MS (Liquid Chromatography Tandem Mass Spectrometer or nanoESI MS/MS) mass spectrometry. Electrospray mass spectrometry cannot be applied to dilute samples and is affected by high salt. As a consequence peptides, normally extracted from acrylamide gels by in situ proteolysis, are desalted and concentrated on a microcolumn followed by elution into a capillary used for nanoelectrospray tandem mass spectrometry. A first mass spectrum (Normal mass spectrum or Q1 mass spectrum) gives information about the masses of all the peptides. Peptides observed in the normal mass spectrum are isolated in turn and dissociated into fragments by collision with gas molecules within the mass spectrometer. Some of the fragments obtained from a peptide constitute a nested set, differing by one amino acid, and the mass difference between them allows assignment of a partial sequence. The masses of the fragments define the position of the partial sequence in the peptide. Together with the cleavage specificity of the protease used to cleave the protein, and mass information such sequence tag provides much higher search specificity to match the a database entry. The procedure is repeated with several peptides from the digest, resulting in multiple identifications of the same protein or identification of several proteins from the peptide mixture. Unknown proteins can easily be identified by using the high specificity of the peptide sequence tag for searches in most sequence databases including EST or genome databases.


Cross References:

Annotations:

Topic
Text
The entry is part of the PrimesDB project, an IMEx observer member.
95 bait proteins were selected based on a highly curated EGFR signaling. The baits were expressed as FLAG-tagged proteins carefully titrating transfection to achieve a similar expression level in both cell lines (HCT116 and HKE3). Baits were immunoprecipitated and associated prey proteins were identified by high- resolution Orbitrap qMS. We defined preys as being “rewired” in EGFRNet mtKRAS-Hi (HCT116 cells), if the abundance of the prey protein in the pull-down was significantly different (P <= 0.05) compared to EGFRNetmtKRAS-Lo (HKE3 cells) and the significance A value for the prey protein was also <= 0.05. Interactions that were identified in only one EGFRNet, but where prey abundance was subsequently not found to be statistically significantly different in the respective bait-prey complexes in the two cell lines were not considered as rewired preys. (Only for 93 baits interactions were found).
imex curation
walter.kolch@ucd.ie,david.lynn@sahmri.com,karsten.boldt@uni-tuebingen.de
2019-12-05: Submitted by David J. Lynn, EMBL Australia Group, Adelaide, Australia

Interaction

Accession: EBI-25380474

Name: shc1-acly-1

Description: -

Type: association

Interaction type: association
Accession: EBI-1813191

Name: association

Description: association

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0914
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Interaction between molecules that may participate in formation of one, but possibly more, physical complexes. Often describes a set of molecules that are co-purified in a single pull-down or coimmunoprecipitation but might participate in formation of distinct physical complexes sharing a common bait.


Cross References:

Database
Identifier
Secondary identifier
Qualifier
-
-

Annotations:

Topic
Text
Suppl Data 2, 4 and 5

Participants (46)

Legend:
A
Annotation and Cross Reference  
P
Experimental Parameter   
S
Stoichiometry   
F
Experimental Feature
C
Participant Confidence
pp123pp
#
Name
Links
Primary Identifier
Aliases
Description
Species
Expression system
Experimental role
Biological role
Interactor type
More...
pp123pp
1
EBI-78835
 logo
SHC1
SHC
SHCA

[+3]
SHC-transforming protein 1
Homo sapiens
Organism Details

Accession: EBI-874

Name: human

Description: Homo sapiens

Cross References:

Database
Identifier
Secondary identifier
Qualifier
human

Expressed In Details

Accession:

Name:

-
bait
Experimental role: bait
Accession: EBI-49

Name: bait

Description: bait

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0496
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Molecule experimentally treated to capture its interacting partners.


unspecified role
Biological role: unspecified role
Accession: EBI-77781

Name: unspecified role

Description: unspecified role

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0499
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Role not specified or not applicable to the data.


protein
Interactor type: protein
Accession: EBI-619654

Name: protein

Description: protein

Cross References:
Database
Identifier
Secondary identifier
Qualifier
so
SO:0000358
-
see-also
psi-mi
MI:0326
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
A linear polymer of amino acids joined by peptide bonds in a specific sequence.


A
Participant: SHC-transforming protein 1

Participant: SHC-transforming protein 1

Accession: EBI-25380478

Name: EBI-78835

P
Participant: SHC-transforming protein 1

Participant: SHC-transforming protein 1

Accession: EBI-25380478

Name: EBI-78835

Parameters:

Type
Value
Unit
Base
Exponent
Uncertainty
No records found.


S
Participant: SHC-transforming protein 1

Participant: SHC-transforming protein 1

Accession: EBI-25380478

Name: EBI-78835

Stoichiometry: 0.0

F
Participant: SHC-transforming protein 1

Participant: SHC-transforming protein 1

Accession: EBI-25380478

Name: EBI-78835

Features:

Legend:
A
Annotation and Cross Reference  
Feature Name
Feature Type
Detection Method
Range positions
More...
flag tag
  • n-n
  • A
    Feature: EBI-25380480

    Feature: flag tag

    Accession: EBI-25380480

    Name: flag tag


    C
    Participant: SHC-transforming protein 1

    Participant: SHC-transforming protein 1

    Accession: EBI-25380478

    Name: EBI-78835

    Confidences:

    Type
    Value
    No records found.

    2
    EBI-2548214
     logo
    ACOT9
    Acyl-CoA thioester hydrolase 9
    CGI-16
    Acyl-coenzyme A thioesterase 9, mitochondrial
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Acyl-coenzyme A thioesterase 9, mitochondrial

    Participant: Acyl-coenzyme A thioesterase 9, mitochondrial

    Accession: EBI-25380482

    Name: EBI-2548214

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HCT116 IP / Prey in control): 1.33

    P
    Participant: Acyl-coenzyme A thioesterase 9, mitochondrial

    Participant: Acyl-coenzyme A thioesterase 9, mitochondrial

    Accession: EBI-25380482

    Name: EBI-2548214

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Acyl-coenzyme A thioesterase 9, mitochondrial

    Participant: Acyl-coenzyme A thioesterase 9, mitochondrial

    Accession: EBI-25380482

    Name: EBI-2548214

    Stoichiometry: 0.0

    F
    Participant: Acyl-coenzyme A thioesterase 9, mitochondrial

    Participant: Acyl-coenzyme A thioesterase 9, mitochondrial

    Accession: EBI-25380482

    Name: EBI-2548214

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Acyl-coenzyme A thioesterase 9, mitochondrial

    Participant: Acyl-coenzyme A thioesterase 9, mitochondrial

    Accession: EBI-25380482

    Name: EBI-2548214

    Confidences:

    Type
    Value
    No records found.

    3
    EBI-1043499
     logo
    Mt-GrpE#1
    HMGE
    GRPEL1

    [+1]
    GrpE protein homolog 1, mitochondrial
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: GrpE protein homolog 1, mitochondrial

    Participant: GrpE protein homolog 1, mitochondrial

    Accession: EBI-25380485

    Name: EBI-1043499

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HCT116 IP / Prey in control): 1.46

    P
    Participant: GrpE protein homolog 1, mitochondrial

    Participant: GrpE protein homolog 1, mitochondrial

    Accession: EBI-25380485

    Name: EBI-1043499

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: GrpE protein homolog 1, mitochondrial

    Participant: GrpE protein homolog 1, mitochondrial

    Accession: EBI-25380485

    Name: EBI-1043499

    Stoichiometry: 0.0

    F
    Participant: GrpE protein homolog 1, mitochondrial

    Participant: GrpE protein homolog 1, mitochondrial

    Accession: EBI-25380485

    Name: EBI-1043499

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: GrpE protein homolog 1, mitochondrial

    Participant: GrpE protein homolog 1, mitochondrial

    Accession: EBI-25380485

    Name: EBI-1043499

    Confidences:

    Type
    Value
    No records found.

    4
    EBI-3440103
     logo
    GAREM1
    C18orf11
    FAM59A

    [+2]
    GRB2-associated and regulator of MAPK protein 1
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: GRB2-associated and regulator of MAPK protein 1

    Participant: GRB2-associated and regulator of MAPK protein 1

    Accession: EBI-25380488

    Name: EBI-3440103

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HCT116 IP / Prey in control): 5.75

    P
    Participant: GRB2-associated and regulator of MAPK protein 1

    Participant: GRB2-associated and regulator of MAPK protein 1

    Accession: EBI-25380488

    Name: EBI-3440103

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: GRB2-associated and regulator of MAPK protein 1

    Participant: GRB2-associated and regulator of MAPK protein 1

    Accession: EBI-25380488

    Name: EBI-3440103

    Stoichiometry: 0.0

    F
    Participant: GRB2-associated and regulator of MAPK protein 1

    Participant: GRB2-associated and regulator of MAPK protein 1

    Accession: EBI-25380488

    Name: EBI-3440103

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: GRB2-associated and regulator of MAPK protein 1

    Participant: GRB2-associated and regulator of MAPK protein 1

    Accession: EBI-25380488

    Name: EBI-3440103

    Confidences:

    Type
    Value
    No records found.

    5
    EBI-297683
     logo
    Adaptor protein complex AP-2 subunit mu
    AP-2 mu chain
    AP2M1

    [+9]
    AP-2 complex subunit mu
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: AP-2 complex subunit mu

    Participant: AP-2 complex subunit mu

    Accession: EBI-25380491

    Name: EBI-297683

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HCT116 IP / Prey in control): 10.47

    P
    Participant: AP-2 complex subunit mu

    Participant: AP-2 complex subunit mu

    Accession: EBI-25380491

    Name: EBI-297683

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: AP-2 complex subunit mu

    Participant: AP-2 complex subunit mu

    Accession: EBI-25380491

    Name: EBI-297683

    Stoichiometry: 0.0

    F
    Participant: AP-2 complex subunit mu

    Participant: AP-2 complex subunit mu

    Accession: EBI-25380491

    Name: EBI-297683

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: AP-2 complex subunit mu

    Participant: AP-2 complex subunit mu

    Accession: EBI-25380491

    Name: EBI-297683

    Confidences:

    Type
    Value
    No records found.

    6
    EBI-302474
     logo
    USP7
    HAUSP
    Ubiquitin thioesterase 7

    [+3]
    Ubiquitin carboxyl-terminal hydrolase 7
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Ubiquitin carboxyl-terminal hydrolase 7

    Participant: Ubiquitin carboxyl-terminal hydrolase 7

    Accession: EBI-25380494

    Name: EBI-302474

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HCT116 IP / Prey in control): 2.13

    P
    Participant: Ubiquitin carboxyl-terminal hydrolase 7

    Participant: Ubiquitin carboxyl-terminal hydrolase 7

    Accession: EBI-25380494

    Name: EBI-302474

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Ubiquitin carboxyl-terminal hydrolase 7

    Participant: Ubiquitin carboxyl-terminal hydrolase 7

    Accession: EBI-25380494

    Name: EBI-302474

    Stoichiometry: 0.0

    F
    Participant: Ubiquitin carboxyl-terminal hydrolase 7

    Participant: Ubiquitin carboxyl-terminal hydrolase 7

    Accession: EBI-25380494

    Name: EBI-302474

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Ubiquitin carboxyl-terminal hydrolase 7

    Participant: Ubiquitin carboxyl-terminal hydrolase 7

    Accession: EBI-25380494

    Name: EBI-302474

    Confidences:

    Type
    Value
    No records found.

    7
    EBI-302524
     logo
    USP9X
    DFFRX
    USP9

    [+7]
    Probable ubiquitin carboxyl-terminal hydrolase FAF-X
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Probable ubiquitin carboxyl-terminal hydrolase FAF-X

    Participant: Probable ubiquitin carboxyl-terminal hydrolase FAF-X

    Accession: EBI-25380497

    Name: EBI-302524

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HCT116 IP / Prey in control): 6.93

    P
    Participant: Probable ubiquitin carboxyl-terminal hydrolase FAF-X

    Participant: Probable ubiquitin carboxyl-terminal hydrolase FAF-X

    Accession: EBI-25380497

    Name: EBI-302524

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Probable ubiquitin carboxyl-terminal hydrolase FAF-X

    Participant: Probable ubiquitin carboxyl-terminal hydrolase FAF-X

    Accession: EBI-25380497

    Name: EBI-302524

    Stoichiometry: 0.0

    F
    Participant: Probable ubiquitin carboxyl-terminal hydrolase FAF-X

    Participant: Probable ubiquitin carboxyl-terminal hydrolase FAF-X

    Accession: EBI-25380497

    Name: EBI-302524

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Probable ubiquitin carboxyl-terminal hydrolase FAF-X

    Participant: Probable ubiquitin carboxyl-terminal hydrolase FAF-X

    Accession: EBI-25380497

    Name: EBI-302524

    Confidences:

    Type
    Value
    No records found.

    8
    EBI-762428
     logo
    Acidic protein rich in leucines
    Silver-stainable protein SSP29
    ANP32B

    [+3]
    Acidic leucine-rich nuclear phosphoprotein 32 family member B
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Acidic leucine-rich nuclear phosphoprotein 32 family member B

    Participant: Acidic leucine-rich nuclear phosphoprotein 32 family member B

    Accession: EBI-25380500

    Name: EBI-762428

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HCT116 IP / Prey in control): 1.33

    P
    Participant: Acidic leucine-rich nuclear phosphoprotein 32 family member B

    Participant: Acidic leucine-rich nuclear phosphoprotein 32 family member B

    Accession: EBI-25380500

    Name: EBI-762428

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Acidic leucine-rich nuclear phosphoprotein 32 family member B

    Participant: Acidic leucine-rich nuclear phosphoprotein 32 family member B

    Accession: EBI-25380500

    Name: EBI-762428

    Stoichiometry: 0.0

    F
    Participant: Acidic leucine-rich nuclear phosphoprotein 32 family member B

    Participant: Acidic leucine-rich nuclear phosphoprotein 32 family member B

    Accession: EBI-25380500

    Name: EBI-762428

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Acidic leucine-rich nuclear phosphoprotein 32 family member B

    Participant: Acidic leucine-rich nuclear phosphoprotein 32 family member B

    Accession: EBI-25380500

    Name: EBI-762428

    Confidences:

    Type
    Value
    No records found.

    9
    EBI-7876312
     logo
    ANKS1B
    Amyloid-beta protein intracellular domain-associated protein 1
    E2A-PBX1-associated protein
    Ankyrin repeat and sterile alpha motif domain-containing protein 1B
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Ankyrin repeat and sterile alpha motif domain-containing protein 1B

    Participant: Ankyrin repeat and sterile alpha motif domain-containing protein 1B

    Accession: EBI-25380503

    Name: EBI-7876312

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HCT116 IP / Prey in control): 9.98

    P
    Participant: Ankyrin repeat and sterile alpha motif domain-containing protein 1B

    Participant: Ankyrin repeat and sterile alpha motif domain-containing protein 1B

    Accession: EBI-25380503

    Name: EBI-7876312

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Ankyrin repeat and sterile alpha motif domain-containing protein 1B

    Participant: Ankyrin repeat and sterile alpha motif domain-containing protein 1B

    Accession: EBI-25380503

    Name: EBI-7876312

    Stoichiometry: 0.0

    F
    Participant: Ankyrin repeat and sterile alpha motif domain-containing protein 1B

    Participant: Ankyrin repeat and sterile alpha motif domain-containing protein 1B

    Accession: EBI-25380503

    Name: EBI-7876312

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Ankyrin repeat and sterile alpha motif domain-containing protein 1B

    Participant: Ankyrin repeat and sterile alpha motif domain-containing protein 1B

    Accession: EBI-25380503

    Name: EBI-7876312

    Confidences:

    Type
    Value
    No records found.

    10
    EBI-1048612
     logo
    Odin
    ANKS1A
    ANKS1

    [+2]
    Ankyrin repeat and SAM domain-containing protein 1A
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Ankyrin repeat and SAM domain-containing protein 1A

    Participant: Ankyrin repeat and SAM domain-containing protein 1A

    Accession: EBI-25380506

    Name: EBI-1048612

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HCT116 IP / Prey in control): 9.98

    P
    Participant: Ankyrin repeat and SAM domain-containing protein 1A

    Participant: Ankyrin repeat and SAM domain-containing protein 1A

    Accession: EBI-25380506

    Name: EBI-1048612

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Ankyrin repeat and SAM domain-containing protein 1A

    Participant: Ankyrin repeat and SAM domain-containing protein 1A

    Accession: EBI-25380506

    Name: EBI-1048612

    Stoichiometry: 0.0

    F
    Participant: Ankyrin repeat and SAM domain-containing protein 1A

    Participant: Ankyrin repeat and SAM domain-containing protein 1A

    Accession: EBI-25380506

    Name: EBI-1048612

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Ankyrin repeat and SAM domain-containing protein 1A

    Participant: Ankyrin repeat and SAM domain-containing protein 1A

    Accession: EBI-25380506

    Name: EBI-1048612

    Confidences:

    Type
    Value
    No records found.

    11
    EBI-356829
     logo
    HSPH1
    HSP105
    HSP110

    [+3]
    Heat shock protein 105 kDa
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Heat shock protein 105 kDa

    Participant: Heat shock protein 105 kDa

    Accession: EBI-25380509

    Name: EBI-356829

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HCT116 IP / Prey in control): 1.75

    P
    Participant: Heat shock protein 105 kDa

    Participant: Heat shock protein 105 kDa

    Accession: EBI-25380509

    Name: EBI-356829

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Heat shock protein 105 kDa

    Participant: Heat shock protein 105 kDa

    Accession: EBI-25380509

    Name: EBI-356829

    Stoichiometry: 0.0

    F
    Participant: Heat shock protein 105 kDa

    Participant: Heat shock protein 105 kDa

    Accession: EBI-25380509

    Name: EBI-356829

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Heat shock protein 105 kDa

    Participant: Heat shock protein 105 kDa

    Accession: EBI-25380509

    Name: EBI-356829

    Confidences:

    Type
    Value
    No records found.

    12
    EBI-395967
     logo
    IPO4
    IMP4B
    RANBP4

    [+2]
    Importin-4
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Importin-4

    Participant: Importin-4

    Accession: EBI-25380512

    Name: EBI-395967

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HCT116 IP / Prey in control): 1.36

    P
    Participant: Importin-4

    Participant: Importin-4

    Accession: EBI-25380512

    Name: EBI-395967

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Importin-4

    Participant: Importin-4

    Accession: EBI-25380512

    Name: EBI-395967

    Stoichiometry: 0.0

    F
    Participant: Importin-4

    Participant: Importin-4

    Accession: EBI-25380512

    Name: EBI-395967

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Importin-4

    Participant: Importin-4

    Accession: EBI-25380512

    Name: EBI-395967

    Confidences:

    Type
    Value
    No records found.

    13
    EBI-5658424
     logo
    C3orf29
    HCV NS5A-transactivated protein 4 splice variant A-binding protein 1
    hJumpy

    [+1]
    Myotubularin-related protein 14
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Myotubularin-related protein 14

    Participant: Myotubularin-related protein 14

    Accession: EBI-25380515

    Name: EBI-5658424

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HCT116 IP / Prey in control): 1.35

    P
    Participant: Myotubularin-related protein 14

    Participant: Myotubularin-related protein 14

    Accession: EBI-25380515

    Name: EBI-5658424

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Myotubularin-related protein 14

    Participant: Myotubularin-related protein 14

    Accession: EBI-25380515

    Name: EBI-5658424

    Stoichiometry: 0.0

    F
    Participant: Myotubularin-related protein 14

    Participant: Myotubularin-related protein 14

    Accession: EBI-25380515

    Name: EBI-5658424

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Myotubularin-related protein 14

    Participant: Myotubularin-related protein 14

    Accession: EBI-25380515

    Name: EBI-5658424

    Confidences:

    Type
    Value
    No records found.

    14
    EBI-1050422
     logo
    LRRK1
    KIAA1790
    Leucine-rich repeat serine/threonine-protein kinase 1
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Leucine-rich repeat serine/threonine-protein kinase 1

    Participant: Leucine-rich repeat serine/threonine-protein kinase 1

    Accession: EBI-25380518

    Name: EBI-1050422

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HCT116 IP / Prey in control): 2.61

    P
    Participant: Leucine-rich repeat serine/threonine-protein kinase 1

    Participant: Leucine-rich repeat serine/threonine-protein kinase 1

    Accession: EBI-25380518

    Name: EBI-1050422

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Leucine-rich repeat serine/threonine-protein kinase 1

    Participant: Leucine-rich repeat serine/threonine-protein kinase 1

    Accession: EBI-25380518

    Name: EBI-1050422

    Stoichiometry: 0.0

    F
    Participant: Leucine-rich repeat serine/threonine-protein kinase 1

    Participant: Leucine-rich repeat serine/threonine-protein kinase 1

    Accession: EBI-25380518

    Name: EBI-1050422

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Leucine-rich repeat serine/threonine-protein kinase 1

    Participant: Leucine-rich repeat serine/threonine-protein kinase 1

    Accession: EBI-25380518

    Name: EBI-1050422

    Confidences:

    Type
    Value
    No records found.

    15
    EBI-350322
     logo
    DDB1
    Damage-specific DNA-binding protein 1
    UV-damaged DNA-binding factor

    [+7]
    DNA damage-binding protein 1
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: DNA damage-binding protein 1

    Participant: DNA damage-binding protein 1

    Accession: EBI-25380521

    Name: EBI-350322

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HCT116 IP / Prey in control): 1.35

    P
    Participant: DNA damage-binding protein 1

    Participant: DNA damage-binding protein 1

    Accession: EBI-25380521

    Name: EBI-350322

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: DNA damage-binding protein 1

    Participant: DNA damage-binding protein 1

    Accession: EBI-25380521

    Name: EBI-350322

    Stoichiometry: 0.0

    F
    Participant: DNA damage-binding protein 1

    Participant: DNA damage-binding protein 1

    Accession: EBI-25380521

    Name: EBI-350322

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: DNA damage-binding protein 1

    Participant: DNA damage-binding protein 1

    Accession: EBI-25380521

    Name: EBI-350322

    Confidences:

    Type
    Value
    No records found.

    16
    EBI-1044041
     logo
    NCAPD2
    CAPD2
    KIAA0159

    [+5]
    Condensin complex subunit 1
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Condensin complex subunit 1

    Participant: Condensin complex subunit 1

    Accession: EBI-25380524

    Name: EBI-1044041

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HCT116 IP / Prey in control): 1.34

    P
    Participant: Condensin complex subunit 1

    Participant: Condensin complex subunit 1

    Accession: EBI-25380524

    Name: EBI-1044041

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Condensin complex subunit 1

    Participant: Condensin complex subunit 1

    Accession: EBI-25380524

    Name: EBI-1044041

    Stoichiometry: 0.0

    F
    Participant: Condensin complex subunit 1

    Participant: Condensin complex subunit 1

    Accession: EBI-25380524

    Name: EBI-1044041

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Condensin complex subunit 1

    Participant: Condensin complex subunit 1

    Accession: EBI-25380524

    Name: EBI-1044041

    Confidences:

    Type
    Value
    No records found.

    17
    EBI-704279
     logo
    nPKC-delta
    PRKCD
    Tyrosine-protein kinase PRKCD
    Protein kinase C delta type
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Protein kinase C delta type

    Participant: Protein kinase C delta type

    Accession: EBI-25380527

    Name: EBI-704279

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HCT116 IP / Prey in control): 4.55

    P
    Participant: Protein kinase C delta type

    Participant: Protein kinase C delta type

    Accession: EBI-25380527

    Name: EBI-704279

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Protein kinase C delta type

    Participant: Protein kinase C delta type

    Accession: EBI-25380527

    Name: EBI-704279

    Stoichiometry: 0.0

    F
    Participant: Protein kinase C delta type

    Participant: Protein kinase C delta type

    Accession: EBI-25380527

    Name: EBI-704279

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Protein kinase C delta type

    Participant: Protein kinase C delta type

    Accession: EBI-25380527

    Name: EBI-704279

    Confidences:

    Type
    Value
    No records found.

    18
    EBI-2266035
     logo
    PTPN12
    Protein-tyrosine phosphatase G1
    PTP-PEST
    Tyrosine-protein phosphatase non-receptor type 12
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Tyrosine-protein phosphatase non-receptor type 12

    Participant: Tyrosine-protein phosphatase non-receptor type 12

    Accession: EBI-25380530

    Name: EBI-2266035

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HCT116 IP / Prey in control): 3.17

    P
    Participant: Tyrosine-protein phosphatase non-receptor type 12

    Participant: Tyrosine-protein phosphatase non-receptor type 12

    Accession: EBI-25380530

    Name: EBI-2266035

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Tyrosine-protein phosphatase non-receptor type 12

    Participant: Tyrosine-protein phosphatase non-receptor type 12

    Accession: EBI-25380530

    Name: EBI-2266035

    Stoichiometry: 0.0

    F
    Participant: Tyrosine-protein phosphatase non-receptor type 12

    Participant: Tyrosine-protein phosphatase non-receptor type 12

    Accession: EBI-25380530

    Name: EBI-2266035

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Tyrosine-protein phosphatase non-receptor type 12

    Participant: Tyrosine-protein phosphatase non-receptor type 12

    Accession: EBI-25380530

    Name: EBI-2266035

    Confidences:

    Type
    Value
    No records found.

    19
    EBI-359174
     logo
    SLC25A11
    SLC20A4
    Solute carrier family 25 member 11
    Mitochondrial 2-oxoglutarate/malate carrier protein
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Mitochondrial 2-oxoglutarate/malate carrier protein

    Participant: Mitochondrial 2-oxoglutarate/malate carrier protein

    Accession: EBI-25380533

    Name: EBI-359174

    Annotations:

    Topic
    Text
    SILAC Ratio (Prey in HCT116 IP / Prey in control): 1.45

    P
    Participant: Mitochondrial 2-oxoglutarate/malate carrier protein

    Participant: Mitochondrial 2-oxoglutarate/malate carrier protein

    Accession: EBI-25380533

    Name: EBI-359174

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Mitochondrial 2-oxoglutarate/malate carrier protein

    Participant: Mitochondrial 2-oxoglutarate/malate carrier protein

    Accession: EBI-25380533

    Name: EBI-359174

    Stoichiometry: 0.0

    F
    Participant: Mitochondrial 2-oxoglutarate/malate carrier protein

    Participant: Mitochondrial 2-oxoglutarate/malate carrier protein

    Accession: EBI-25380533

    Name: EBI-359174

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Mitochondrial 2-oxoglutarate/malate carrier protein

    Participant: Mitochondrial 2-oxoglutarate/malate carrier protein

    Accession: EBI-25380533

    Name: EBI-359174

    Confidences:

    Type
    Value
    No records found.

    20
    EBI-2562430
     logo
    AKAP12
    AKAP250
    A-kinase anchor protein 250 kDa

    [+2]
    A-kinase anchor protein 12
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: A-kinase anchor protein 12

    Participant: A-kinase anchor protein 12

    Accession: EBI-25380536

    Name: EBI-2562430

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    -

    Annotations:

    Topic
    Text
    Prey abundance was significantly different between HCT116 and HKE3 cells.
    SILAC Ratio (Prey in HCT116 IP / Prey in control): 1.43
    SILAC Ratio (Prey in HCT116 IP / Prey in HKE3 IP): 1.86

    P
    Participant: A-kinase anchor protein 12

    Participant: A-kinase anchor protein 12

    Accession: EBI-25380536

    Name: EBI-2562430

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: A-kinase anchor protein 12

    Participant: A-kinase anchor protein 12

    Accession: EBI-25380536

    Name: EBI-2562430

    Stoichiometry: 0.0

    F
    Participant: A-kinase anchor protein 12

    Participant: A-kinase anchor protein 12

    Accession: EBI-25380536

    Name: EBI-2562430

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: A-kinase anchor protein 12

    Participant: A-kinase anchor protein 12

    Accession: EBI-25380536

    Name: EBI-2562430

    Confidences:

    Type
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    Graphical Representation of Experimental Features