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What is this view?

Binary interactions

In this tab, we display the list of interactions that you have selected using one of our search features. Despite the fact that our data are annotated to accurately reflect the interactions reported in scientific literature, the data is shown in this view as binary interactions. Whenever the data was reported as a co-complex involving more than two molecules, we store it as such in the IntAct database and post-process it so the portal can show it as binary interaction. This post-processing is the Spoke Expansion model (connects bait to all preys):



sourceExp

At any moment you can choose to display the expansion column in this view in order to see which interaction are spoke expanded and which are not.

Description of what has changed

  • We have added more download options to allow users to retrieve their interaction set using more standard formats such as PSI-MI XML and PSIMITAB (version 2.5, 2.6 or 2.7) but also XGMML, RDF and Biopax (level 2 and 3).
  • We have now four different table views : minimal(molecule names and interaction AC), basic (minimal + molecule links, interaction detection method, negative), standard(minimal + molecule species, confidences, publication details, experiment details), expanded (standard + more experiment details) and complete (all mitab 2.7 columns).

Configuring the view to your need"

In the header of the interaction table you will find a button: ‘Change Column Display’ that will show you all the columns/Table views available and allow you to update the current selected set.

Downloading the data into Standard formats"

In the header of the interaction table you will find a drop down list that contains all the formats currently supported when downloading the interaction data. Select one of them and click the export button next to the list. Please note that PSI-MI XML is only available when the interaction set is no bigger than 1000 interactions.

Opening the interaction details"

Clicking on the magnifying glass in the first column of the interaction table will open the details of the corresponding interaction in the Interaction Details tab, giving you access to more details of the manually curated record.

What is this view?

Browsing (Browse Tab)

This tab is meant to give you access to more content based on the currently selected set of interactions. Please note that linking to third party resources will only include up to 200 molecules , if you exceed this number you will see the warning icon (This number has been reduced to 125 molecules for mRNA expression). Now let’s look at the features available to you:

Limiting the scope of the current dataset with the Uniprot Taxonomy ontology

Allows users to browse the Uniprot Taxonomy hierarchy as a tree and select terms in order to narrow down their dataset. Once a term is selected, you are taken back to the interaction tab to review your dataset.

Limiting the scope of the current dataset with the GO ontology

Allows users to browse the GO hierarchy as a tree and select terms in order to narrow down their dataset. Once a term is selected, you are taken back to the interaction tab to review your dataset.

Limiting the scope of the current dataset with the ChEBI ontology

Allows users to browse the ChEBI hierarchy as a tree and select terms in order to narrow down their dataset. Once a term is selected, you are taken back to the interaction tab to review your dataset.

Bulk linking to third party resources by using involved proteins

  • Proteins by Reactome pathway: Sends your proteins to the Reactome SkyPainter that will show you the pathways in which these molecules are know to play a role.
  • Proteins by Chromosomal location: Sends your list of proteins to Ensembl’s Karyotype viewer and overlays the proteins on the chromosomes.
  • Proteins by mRNA expression: Sends your set of proteins to the ArrayExpress Atlas that will show the known gene expression based on experimental studies.
What is this view?

Searching Interactions (Search Tab)

As you can see in this tab we are now trying to give you more targeted choice to do your queries, please note that the examples provided in this tab are live links so you can simply click them to see the resulting interactions sets.

Using the Quick Search

In this search panel you are free to type anything that might relate to interactions, whether it is properties of their interactor (gene name, identifiers, GO term…) or more specific to the interaction like publication, authors, experimental detection method, ...

Some examples:

  • Try the query: imatinib
    This is a drug for which we have curated a number of interactions.
    Once you press the search button you should be taken to the Interaction Tab that lists 130 binary interactions.
    If you want to construct more complex queries we recommend you take a look at the Molecular Interaction Query Language, accessible from the quick search panel.
  • Try the query: species:yeast AND type:"direct interaction"
    This query selects all interactions involving yeast interactors that have been shown to have direct interactions. If you customize the column display of the interaction tab, you will see that not only “direct interaction” have been selected but also children terms in the PSI-MI ontology.

Using the Ontology Search

Open the Rearch Tab. This panel is specialised to give you an easy access to ontology search. So far you can search on 4 ontologies:

  • Gene Ontology
  • InterPro
  • PSI-MI
  • ChEBI

Whenever you start typing a query in this search panel, the system will search as you type and propose a list of matching controlled vocabulary terms. You can then select one of them and select matching interactions.

For example, type: cancer
You will be presented with a few choices, please note that each term is followed by the count of matching interactions in the IntAct database.

Select a term with the mouse or using the keyboard cursor keys and you will be taken to the interaction tab.

Searching the Compound chemical structure

In this panel you will be able to draw all or part of a chemical structure and search for chemical compounds. If you get any matched, you can then see all interactions involving them.

First you have to open up the chemical search panel so that the applet can load, it might take a few seconds. Then you can start drawing your structure, for instance:

Once you have drawn your structure, select Similarity and press Search. You should be presented with a list of matching compound. Now choose one molecule and click the link: IntAct interactions. You will be taken to the interaction tab to review the data.

Complex Expansion

Binary interactions generated by co-complex expansion

Why should you care about complex expansion ?

Some experimental methods such as Tandem Affinity Purification do generate molecular interactions that can involve more than 2 molecules. Despite the fact that IntAct curation team do capture the molecular interaction as they were reported in the corresponding experiment, when you search using the intact web site, the results of your query is always shown as set of binary interactions (i.e. 2 molecules). We would like to draw your attention on the fact that whenever the reported interaction was a co-complex we do apply an expansion algorithm that transform this n-ary interaction into a set of binary interactions. While none of these agorithms is perfect and will very likely generate some false positive interactions, it is useful to present the data in a consistent manner. Bear in mind that we will strive to differentiate in the search results which interactions are a real experimental binary from expanded ones.

Existing expansion algorithm

There are several known algorithm allowing to transform an n-ary interaction into a set of binaries. The illustration below present the two well known expansion model and illustrates why they can be incorrect.



sourceExp

  • Spoke expansion: Links the bait molecule to all prey molecules. If N is the count of molecule in the complex, it generated N-1 binary interactions.
  • Matrix expansion: Links all molecule to all other molecule present in the complex. If N is the count of molecule in the complex, it generated (N*(N-1))/2 binary interactions.

Now the issue (as illustrated at the bottom right of the diagram above) with these two models lies in the fact that the real complex might not be articulated around the experimental bait but instead, this bait might be linked to a smaller complex, hence most binary interaction generated by spoke and matrix expansion result in false positive.



PSICQUIC

How is the number of interactions in other databases obtained?

PSICQUIC is a standard way to access molecular interaction databases across which it repeats the same query. The number of databases providing data may vary, depending on the status of their services and only those that are active are used in this query. By clicking on the number of interactions you will be redirected to the PSICQUIC View, where you can browse the results in those other resources.

The services currently active are:

Check the PSICQUIC site for more information.

IMEx

What is the significance of the IMEx dataset?"

IMEx is a network of databases which have agreed to supply a non-redundant set of data expertly manually annotated to the same consistent detailed standard which, as such, represents a high-quality subset of the data each individually provides. The number of databases providing data may vary, depending on the status of their services and only those that are active are used in this query. By clicking on the number of interactions you will be redirected to the IMEx View, where you can browse the results in those other resources.

The services currently active are:

Check the IMEx site for more information.

What is this view?

Representation of Experimental Features

This section shows the graphical representation of experimental features, where each participant is represented as a white rectangle with a black border and a line for each hundredth amino acid. All available features are attached to their associated participant and their categories are represented in the right side of the legend. The left side of the legend dynamically shows the range statuses occuring in the shown interaction. These are the possible range statuses:

sourceExp

Interacting with the widget

Hover over a feature to see more information in a tooltip.
sourceExp

To display a single interacting region click on it and click again to display all interacting regions.
Displaying all interacting regionsDisplaying one interacting region
sourceExpsourceExp
What is this view?

Dynamic molecular interaction data

This section shows the graphical representation of dynamic molecular interactions. By default it displays all the interactions from one experiment using radio buttons to allow users to highlight interactions in different variable conditions.

Publication

PubMed Id: 28514442

Journal: Nature

Year of Publication: 2017

Title: Architecture of the human interactome defines protein communities and disease networks.

Author List: Huttlin EL., Bruckner RJ., Paulo JA., Cannon JR., Ting L., Baltier K., Colby G., Gebreab F., Gygi MP., Parzen H., Szpyt J., Tam S., Zarraga G., Pontano-Vaites L., Swarup S., White AE., Schweppe DK., Rad R., Erickson BK., Obar RA., Guruharsha KG., Li K., Artavanis-Tsakonas S., Gygi SP., Harper JW.

Cross References:

Experiment (5110 interactions)

Accession: EBI-21498811

Name: huttlin-2017-3

Host organism: Homo sapiens 293 cells transformed with SV40 large T antigen

Organism: Homo sapiens 293 cells transformed with SV40 large T antigen

Accession: EBI-308002

Name: human-293t

Description: Homo sapiens 293 cells transformed with SV40 large T antigen

Cross References:

Database
Identifier
Secondary identifier
Qualifier
human
-

Interaction Detection Method: anti tag coip

Interaction detection method: anti tag coip
Accession: EBI-90

Name: anti tag coip

Description: anti tag coimmunoprecipitation

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0007
-
identity
pubmed
7708014
-
primary-reference


Annotations:
Topic
Text
definition
A specific antibody for the molecule of interest is not available, therefore the bait protein is expressed as a hybrid protein fused to a tag peptide/protein for which efficient and specific antibodies or a specific ligand are available.


Participant Identification Method: sequence tag

Participant identification method: sequence tag
Accession: EBI-81

Name: sequence tag

Description: sequence tag identification

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0102
-
identity
pubmed
10967324
-
primary-reference
pubmed
11752590
-
primary-reference
pubmed
11805837
-
primary-reference


Annotations:
Topic
Text
definition
This approach leads to protein identification by combining mass measurement and short amino acid sequence information obtained by tandem mass spectrometry. This information is then used to automatically find the best match in a sequence database. A mixture of peptides derived from a protease digestion is analysed by nanoelectrospray LC-MS/MS (Liquid Chromatography Tandem Mass Spectrometer or nanoESI MS/MS) mass spectrometry. Electrospray mass spectrometry cannot be applied to dilute samples and is affected by high salt. As a consequence peptides, normally extracted from acrylamide gels by in situ proteolysis, are desalted and concentrated on a microcolumn followed by elution into a capillary used for nanoelectrospray tandem mass spectrometry. A first mass spectrum (Normal mass spectrum or Q1 mass spectrum) gives information about the masses of all the peptides. Peptides observed in the normal mass spectrum are isolated in turn and dissociated into fragments by collision with gas molecules within the mass spectrometer. Some of the fragments obtained from a peptide constitute a nested set, differing by one amino acid, and the mass difference between them allows assignment of a partial sequence. The masses of the fragments define the position of the partial sequence in the peptide. Together with the cleavage specificity of the protease used to cleave the protein, and mass information such sequence tag provides much higher search specificity to match the a database entry. The procedure is repeated with several peptides from the digest, resulting in multiple identifications of the same protein or identification of several proteins from the peptide mixture. Unknown proteins can easily be identified by using the high specificity of the peptide sequence tag for searches in most sequence databases including EST or genome databases.


Cross References:

Annotations:

Topic
Text
Experimental roles have been inferred upon counting participant occurrences and might not reflect the actual constructs used in the original experiments. Interactions depicted in this experiment may be an aggregate from different co-immunoprecipitations, using bait constructs that may or may not represent the full length sequence of the referenced protein.
edward_huttlin@hms.harvard.edu,steven.gygi@hms.harvard.edu,wade_harper@hms.harvard.edu
imex curation

Interaction

Accession: EBI-21834089

Name: vim-des-1

Description: -

Type: association

Interaction type: association
Accession: EBI-1813191

Name: association

Description: association

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0914
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Interaction between molecules that may participate in formation of one, but possibly more, physical complexes. Often describes a set of molecules that are co-purified in a single pull-down or coimmunoprecipitation but might participate in formation of distinct physical complexes sharing a common bait.


Cross References:

Database
Identifier
Secondary identifier
Qualifier
-

Annotations:

Topic
Text
Supplementary table 1

Participants (12)

Legend:
A
Annotation and Cross Reference  
P
Experimental Parameter   
S
Stoichiometry   
F
Experimental Feature
C
Participant Confidence
#
Name
Links
Primary Identifier
Aliases
Description
Species
Expression system
Experimental role
Biological role
Interactor type
More...
1
EBI-353844
 logo
VIM
Vimentin
Homo sapiens
Organism Details

Accession: EBI-874

Name: human

Description: Homo sapiens

Cross References:

Database
Identifier
Secondary identifier
Qualifier
human

Expressed In Details

Accession:

Name:

-
bait
Experimental role: bait
Accession: EBI-49

Name: bait

Description: bait

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0496
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Molecule experimentally treated to capture its interacting partners.


unspecified role
Biological role: unspecified role
Accession: EBI-77781

Name: unspecified role

Description: unspecified role

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0499
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Role not specified or not applicable to the data.


protein
Interactor type: protein
Accession: EBI-619654

Name: protein

Description: protein

Cross References:
Database
Identifier
Secondary identifier
Qualifier
so
SO:0000358
-
see-also
psi-mi
MI:0326
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
A linear polymer of amino acids joined by peptide bonds in a specific sequence.


A
Participant: Vimentin

Participant: Vimentin

Accession: EBI-21834091

Name: EBI-353844

Cross References:

Database
Identifier
Secondary identifier
Qualifier
-

P
Participant: Vimentin

Participant: Vimentin

Accession: EBI-21834091

Name: EBI-353844

Parameters:

Type
Value
Unit
Base
Exponent
Uncertainty
No records found.


S
Participant: Vimentin

Participant: Vimentin

Accession: EBI-21834091

Name: EBI-353844

Stoichiometry: 0.0

F
Participant: Vimentin

Participant: Vimentin

Accession: EBI-21834091

Name: EBI-353844

Features:

Legend:
A
Annotation and Cross Reference  
Feature Name
Feature Type
Detection Method
Range positions
More...
C-terminal HA-flag tag
  • c-c
  • A
    Feature: EBI-21834093

    Feature: C-terminal HA-flag tag

    Accession: EBI-21834093

    Name: C-terminal HA-flag tag


    C
    Participant: Vimentin

    Participant: Vimentin

    Accession: EBI-21834091

    Name: EBI-353844

    Confidences:

    Type
    Value
    No records found.

    2
    EBI-475646
     logo
    Neurofilament triplet L protein
    68 kDa neurofilament protein
    NEFL

    [+2]
    Neurofilament light polypeptide
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Neurofilament light polypeptide

    Participant: Neurofilament light polypeptide

    Accession: EBI-21834096

    Name: EBI-475646

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    -

    P
    Participant: Neurofilament light polypeptide

    Participant: Neurofilament light polypeptide

    Accession: EBI-21834096

    Name: EBI-475646

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Neurofilament light polypeptide

    Participant: Neurofilament light polypeptide

    Accession: EBI-21834096

    Name: EBI-475646

    Stoichiometry: 0.0

    F
    Participant: Neurofilament light polypeptide

    Participant: Neurofilament light polypeptide

    Accession: EBI-21834096

    Name: EBI-475646

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Neurofilament light polypeptide

    Participant: Neurofilament light polypeptide

    Accession: EBI-21834096

    Name: EBI-475646

    Confidences:

    Type
    Value
    Specific binding CompPASS-Plus score: 0.99996937190774005
    Non-specific binding CompPASS-Plus score: 3.0628092263057998E-5
    False-positive CompPASS-Plus score: 6.5550960836537999E-26

    3
    EBI-1105035
     logo
    NEFM
    NEF3
    NFM

    [+3]
    Neurofilament medium polypeptide
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Neurofilament medium polypeptide

    Participant: Neurofilament medium polypeptide

    Accession: EBI-21834102

    Name: EBI-1105035

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    -

    P
    Participant: Neurofilament medium polypeptide

    Participant: Neurofilament medium polypeptide

    Accession: EBI-21834102

    Name: EBI-1105035

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Neurofilament medium polypeptide

    Participant: Neurofilament medium polypeptide

    Accession: EBI-21834102

    Name: EBI-1105035

    Stoichiometry: 0.0

    F
    Participant: Neurofilament medium polypeptide

    Participant: Neurofilament medium polypeptide

    Accession: EBI-21834102

    Name: EBI-1105035

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Neurofilament medium polypeptide

    Participant: Neurofilament medium polypeptide

    Accession: EBI-21834102

    Name: EBI-1105035

    Confidences:

    Type
    Value
    Specific binding CompPASS-Plus score: 0.99999974606624997
    Non-specific binding CompPASS-Plus score: 2.5393374564178001E-7
    False-positive CompPASS-Plus score: 7.4390985440714001E-29

    4
    EBI-21600741
     logo
    GFAP
    Glial fibrillary acidic protein
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Glial fibrillary acidic protein

    Participant: Glial fibrillary acidic protein

    Accession: EBI-21834108

    Name: EBI-21600741

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    -

    P
    Participant: Glial fibrillary acidic protein

    Participant: Glial fibrillary acidic protein

    Accession: EBI-21834108

    Name: EBI-21600741

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Glial fibrillary acidic protein

    Participant: Glial fibrillary acidic protein

    Accession: EBI-21834108

    Name: EBI-21600741

    Stoichiometry: 0.0

    F
    Participant: Glial fibrillary acidic protein

    Participant: Glial fibrillary acidic protein

    Accession: EBI-21834108

    Name: EBI-21600741

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Glial fibrillary acidic protein

    Participant: Glial fibrillary acidic protein

    Accession: EBI-21834108

    Name: EBI-21600741

    Confidences:

    Type
    Value
    Specific binding CompPASS-Plus score: 0.99993591360802003
    Non-specific binding CompPASS-Plus score: 6.4086391983027996E-5
    False-positive CompPASS-Plus score: 3.0105475702754E-17

    5
    EBI-2556886
     logo
    IDE
    Insulin protease
    Insulysin

    [+1]
    Insulin-degrading enzyme
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Insulin-degrading enzyme

    Participant: Insulin-degrading enzyme

    Accession: EBI-21834114

    Name: EBI-2556886

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    -

    P
    Participant: Insulin-degrading enzyme

    Participant: Insulin-degrading enzyme

    Accession: EBI-21834114

    Name: EBI-2556886

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Insulin-degrading enzyme

    Participant: Insulin-degrading enzyme

    Accession: EBI-21834114

    Name: EBI-2556886

    Stoichiometry: 0.0

    F
    Participant: Insulin-degrading enzyme

    Participant: Insulin-degrading enzyme

    Accession: EBI-21834114

    Name: EBI-2556886

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Insulin-degrading enzyme

    Participant: Insulin-degrading enzyme

    Accession: EBI-21834114

    Name: EBI-2556886

    Confidences:

    Type
    Value
    Specific binding CompPASS-Plus score: 0.78679823305858898
    Non-specific binding CompPASS-Plus score: 0.213201766930393
    False-positive CompPASS-Plus score: 1.1017847642141301E-11

    6
    EBI-1055572
     logo
    DES
    Desmin
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Desmin

    Participant: Desmin

    Accession: EBI-21834120

    Name: EBI-1055572

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    -

    P
    Participant: Desmin

    Participant: Desmin

    Accession: EBI-21834120

    Name: EBI-1055572

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Desmin

    Participant: Desmin

    Accession: EBI-21834120

    Name: EBI-1055572

    Stoichiometry: 0.0

    F
    Participant: Desmin

    Participant: Desmin

    Accession: EBI-21834120

    Name: EBI-1055572

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Desmin

    Participant: Desmin

    Accession: EBI-21834120

    Name: EBI-1055572

    Confidences:

    Type
    Value
    Specific binding CompPASS-Plus score: 0.99999956208549401
    Non-specific binding CompPASS-Plus score: 1.7043885467491801E-9
    False-positive CompPASS-Plus score: 4.3621011751564598E-7

    7
    EBI-10966836
     logo
    NES
    Nbla00170
    Nestin
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Nestin

    Participant: Nestin

    Accession: EBI-21834126

    Name: EBI-10966836

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    -

    P
    Participant: Nestin

    Participant: Nestin

    Accession: EBI-21834126

    Name: EBI-10966836

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Nestin

    Participant: Nestin

    Accession: EBI-21834126

    Name: EBI-10966836

    Stoichiometry: 0.0

    F
    Participant: Nestin

    Participant: Nestin

    Accession: EBI-21834126

    Name: EBI-10966836

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Nestin

    Participant: Nestin

    Accession: EBI-21834126

    Name: EBI-10966836

    Confidences:

    Type
    Value
    Specific binding CompPASS-Plus score: 0.99999828576321204
    Non-specific binding CompPASS-Plus score: 1.71008379774293E-6
    False-positive CompPASS-Plus score: 4.15299062888255E-9

    8
    EBI-297954
     logo
    EPPK1
    EPIPL
    450 kDa epidermal antigen
    Epiplakin
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Epiplakin

    Participant: Epiplakin

    Accession: EBI-21834132

    Name: EBI-297954

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    -

    P
    Participant: Epiplakin

    Participant: Epiplakin

    Accession: EBI-21834132

    Name: EBI-297954

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Epiplakin

    Participant: Epiplakin

    Accession: EBI-21834132

    Name: EBI-297954

    Stoichiometry: 0.0

    F
    Participant: Epiplakin

    Participant: Epiplakin

    Accession: EBI-21834132

    Name: EBI-297954

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Epiplakin

    Participant: Epiplakin

    Accession: EBI-21834132

    Name: EBI-297954

    Confidences:

    Type
    Value
    Specific binding CompPASS-Plus score: 0.84471048032291096
    Non-specific binding CompPASS-Plus score: 0.15528951964823301
    False-positive CompPASS-Plus score: 2.88554482501423E-11

    9
    EBI-366258
     logo
    INA
    66 kDa neurofilament protein
    Neurofilament 5

    [+1]
    Alpha-internexin
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Alpha-internexin

    Participant: Alpha-internexin

    Accession: EBI-21834138

    Name: EBI-366258

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    -

    P
    Participant: Alpha-internexin

    Participant: Alpha-internexin

    Accession: EBI-21834138

    Name: EBI-366258

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Alpha-internexin

    Participant: Alpha-internexin

    Accession: EBI-21834138

    Name: EBI-366258

    Stoichiometry: 0.0

    F
    Participant: Alpha-internexin

    Participant: Alpha-internexin

    Accession: EBI-21834138

    Name: EBI-366258

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Alpha-internexin

    Participant: Alpha-internexin

    Accession: EBI-21834138

    Name: EBI-366258

    Confidences:

    Type
    Value
    Specific binding CompPASS-Plus score: 0.99999670941974494
    Non-specific binding CompPASS-Plus score: 3.2905771294234799E-6
    False-positive CompPASS-Plus score: 3.1256348063080101E-12

    10
    EBI-711736
     logo
    N-recognin-1
    Ubiquitin-protein ligase E3-alpha-1
    Ubiquitin-protein ligase E3-alpha-I

    [+2]
    E3 ubiquitin-protein ligase UBR1
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: E3 ubiquitin-protein ligase UBR1

    Participant: E3 ubiquitin-protein ligase UBR1

    Accession: EBI-21834144

    Name: EBI-711736

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    -

    P
    Participant: E3 ubiquitin-protein ligase UBR1

    Participant: E3 ubiquitin-protein ligase UBR1

    Accession: EBI-21834144

    Name: EBI-711736

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: E3 ubiquitin-protein ligase UBR1

    Participant: E3 ubiquitin-protein ligase UBR1

    Accession: EBI-21834144

    Name: EBI-711736

    Stoichiometry: 0.0

    F
    Participant: E3 ubiquitin-protein ligase UBR1

    Participant: E3 ubiquitin-protein ligase UBR1

    Accession: EBI-21834144

    Name: EBI-711736

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: E3 ubiquitin-protein ligase UBR1

    Participant: E3 ubiquitin-protein ligase UBR1

    Accession: EBI-21834144

    Name: EBI-711736

    Confidences:

    Type
    Value
    Specific binding CompPASS-Plus score: 0.92244954016559699
    Non-specific binding CompPASS-Plus score: 7.7550459818429396E-2
    False-positive CompPASS-Plus score: 1.5973710344727501E-11

    11
    EBI-2341274
     logo
    RSPRY1
    KIAA1972
    UNQ328/PRO444
    RING finger and SPRY domain-containing protein 1
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: RING finger and SPRY domain-containing protein 1

    Participant: RING finger and SPRY domain-containing protein 1

    Accession: EBI-21834150

    Name: EBI-2341274

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    -

    P
    Participant: RING finger and SPRY domain-containing protein 1

    Participant: RING finger and SPRY domain-containing protein 1

    Accession: EBI-21834150

    Name: EBI-2341274

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: RING finger and SPRY domain-containing protein 1

    Participant: RING finger and SPRY domain-containing protein 1

    Accession: EBI-21834150

    Name: EBI-2341274

    Stoichiometry: 0.0

    F
    Participant: RING finger and SPRY domain-containing protein 1

    Participant: RING finger and SPRY domain-containing protein 1

    Accession: EBI-21834150

    Name: EBI-2341274

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: RING finger and SPRY domain-containing protein 1

    Participant: RING finger and SPRY domain-containing protein 1

    Accession: EBI-21834150

    Name: EBI-2341274

    Confidences:

    Type
    Value
    Specific binding CompPASS-Plus score: 0.99037023228216003
    Non-specific binding CompPASS-Plus score: 9.6297677178435997E-3
    False-positive CompPASS-Plus score: 6.7488122743561003E-21

    12
    EBI-764342
     logo
    Kelch-like protein 16
    GAN
    GAN1

    [+1]
    Gigaxonin
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Gigaxonin

    Participant: Gigaxonin

    Accession: EBI-21834156

    Name: EBI-764342

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    -

    P
    Participant: Gigaxonin

    Participant: Gigaxonin

    Accession: EBI-21834156

    Name: EBI-764342

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Gigaxonin

    Participant: Gigaxonin

    Accession: EBI-21834156

    Name: EBI-764342

    Stoichiometry: 0.0

    F
    Participant: Gigaxonin

    Participant: Gigaxonin

    Accession: EBI-21834156

    Name: EBI-764342

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Gigaxonin

    Participant: Gigaxonin

    Accession: EBI-21834156

    Name: EBI-764342

    Confidences:

    Type
    Value
    Specific binding CompPASS-Plus score: 0.99809213299424004
    Non-specific binding CompPASS-Plus score: 1.9078670057556999E-3
    False-positive CompPASS-Plus score: 8.7584961340663993E-28


    Graphical Representation of Experimental Features