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What is this view?

Binary interactions

In this tab, we display the list of interactions that you have selected using one of our search features. Despite the fact that our data are annotated to accurately reflect the interactions reported in scientific literature, the data is shown in this view as binary interactions. Whenever the data was reported as a co-complex involving more than two molecules, we store it as such in the IntAct database and post-process it so the portal can show it as binary interaction. This post-processing is the Spoke Expansion model (connects bait to all preys):



sourceExp

At any moment you can choose to display the expansion column in this view in order to see which interaction are spoke expanded and which are not.

Description of what has changed

  • We have added more download options to allow users to retrieve their interaction set using more standard formats such as PSI-MI XML and PSIMITAB (version 2.5, 2.6 or 2.7) but also XGMML, RDF and Biopax (level 2 and 3).
  • We have now four different table views : minimal(molecule names and interaction AC), basic (minimal + molecule links, interaction detection method, negative), standard(minimal + molecule species, confidences, publication details, experiment details), expanded (standard + more experiment details) and complete (all mitab 2.7 columns).

Configuring the view to your need"

In the header of the interaction table you will find a button: ‘Change Column Display’ that will show you all the columns/Table views available and allow you to update the current selected set.

Downloading the data into Standard formats"

In the header of the interaction table you will find a drop down list that contains all the formats currently supported when downloading the interaction data. Select one of them and click the export button next to the list. Please note that PSI-MI XML is only available when the interaction set is no bigger than 1000 interactions.

Opening the interaction details"

Clicking on the magnifying glass in the first column of the interaction table will open the details of the corresponding interaction in the Interaction Details tab, giving you access to more details of the manually curated record.

What is this view?

Browsing (Browse Tab)

This tab is meant to give you access to more content based on the currently selected set of interactions. Please note that linking to third party resources will only include up to 200 molecules , if you exceed this number you will see the warning icon (This number has been reduced to 125 molecules for mRNA expression). Now let’s look at the features available to you:

Limiting the scope of the current dataset with the Uniprot Taxonomy ontology

Allows users to browse the Uniprot Taxonomy hierarchy as a tree and select terms in order to narrow down their dataset. Once a term is selected, you are taken back to the interaction tab to review your dataset.

Limiting the scope of the current dataset with the GO ontology

Allows users to browse the GO hierarchy as a tree and select terms in order to narrow down their dataset. Once a term is selected, you are taken back to the interaction tab to review your dataset.

Limiting the scope of the current dataset with the ChEBI ontology

Allows users to browse the ChEBI hierarchy as a tree and select terms in order to narrow down their dataset. Once a term is selected, you are taken back to the interaction tab to review your dataset.

Bulk linking to third party resources by using involved proteins

  • Proteins by Reactome pathway: Sends your proteins to the Reactome SkyPainter that will show you the pathways in which these molecules are know to play a role.
  • Proteins by Chromosomal location: Sends your list of proteins to Ensembl’s Karyotype viewer and overlays the proteins on the chromosomes.
  • Proteins by mRNA expression: Sends your set of proteins to the ArrayExpress Atlas that will show the known gene expression based on experimental studies.
What is this view?

Searching Interactions (Search Tab)

As you can see in this tab we are now trying to give you more targeted choice to do your queries, please note that the examples provided in this tab are live links so you can simply click them to see the resulting interactions sets.

Using the Quick Search

In this search panel you are free to type anything that might relate to interactions, whether it is properties of their interactor (gene name, identifiers, GO term…) or more specific to the interaction like publication, authors, experimental detection method, ...

Some examples:

  • Try the query: imatinib
    This is a drug for which we have curated a number of interactions.
    Once you press the search button you should be taken to the Interaction Tab that lists 130 binary interactions.
    If you want to construct more complex queries we recommend you take a look at the Molecular Interaction Query Language, accessible from the quick search panel.
  • Try the query: species:yeast AND type:"direct interaction"
    This query selects all interactions involving yeast interactors that have been shown to have direct interactions. If you customize the column display of the interaction tab, you will see that not only “direct interaction” have been selected but also children terms in the PSI-MI ontology.

Using the Ontology Search

Open the Rearch Tab. This panel is specialised to give you an easy access to ontology search. So far you can search on 4 ontologies:

  • Gene Ontology
  • InterPro
  • PSI-MI
  • ChEBI

Whenever you start typing a query in this search panel, the system will search as you type and propose a list of matching controlled vocabulary terms. You can then select one of them and select matching interactions.

For example, type: cancer
You will be presented with a few choices, please note that each term is followed by the count of matching interactions in the IntAct database.

Select a term with the mouse or using the keyboard cursor keys and you will be taken to the interaction tab.

Searching the Compound chemical structure

In this panel you will be able to draw all or part of a chemical structure and search for chemical compounds. If you get any matched, you can then see all interactions involving them.

First you have to open up the chemical search panel so that the applet can load, it might take a few seconds. Then you can start drawing your structure, for instance:

Once you have drawn your structure, select Similarity and press Search. You should be presented with a list of matching compound. Now choose one molecule and click the link: IntAct interactions. You will be taken to the interaction tab to review the data.

Complex Expansion

Binary interactions generated by co-complex expansion

Why should you care about complex expansion ?

Some experimental methods such as Tandem Affinity Purification do generate molecular interactions that can involve more than 2 molecules. Despite the fact that IntAct curation team do capture the molecular interaction as they were reported in the corresponding experiment, when you search using the intact web site, the results of your query is always shown as set of binary interactions (i.e. 2 molecules). We would like to draw your attention on the fact that whenever the reported interaction was a co-complex we do apply an expansion algorithm that transform this n-ary interaction into a set of binary interactions. While none of these agorithms is perfect and will very likely generate some false positive interactions, it is useful to present the data in a consistent manner. Bear in mind that we will strive to differentiate in the search results which interactions are a real experimental binary from expanded ones.

Existing expansion algorithm

There are several known algorithm allowing to transform an n-ary interaction into a set of binaries. The illustration below present the two well known expansion model and illustrates why they can be incorrect.



sourceExp

  • Spoke expansion: Links the bait molecule to all prey molecules. If N is the count of molecule in the complex, it generated N-1 binary interactions.
  • Matrix expansion: Links all molecule to all other molecule present in the complex. If N is the count of molecule in the complex, it generated (N*(N-1))/2 binary interactions.

Now the issue (as illustrated at the bottom right of the diagram above) with these two models lies in the fact that the real complex might not be articulated around the experimental bait but instead, this bait might be linked to a smaller complex, hence most binary interaction generated by spoke and matrix expansion result in false positive.



PSICQUIC

How is the number of interactions in other databases obtained?

PSICQUIC is a standard way to access molecular interaction databases across which it repeats the same query. The number of databases providing data may vary, depending on the status of their services and only those that are active are used in this query. By clicking on the number of interactions you will be redirected to the PSICQUIC View, where you can browse the results in those other resources.

The services currently active are:

Check the PSICQUIC site for more information.

IMEx

What is the significance of the IMEx dataset?"

IMEx is a network of databases which have agreed to supply a non-redundant set of data expertly manually annotated to the same consistent detailed standard which, as such, represents a high-quality subset of the data each individually provides. The number of databases providing data may vary, depending on the status of their services and only those that are active are used in this query. By clicking on the number of interactions you will be redirected to the IMEx View, where you can browse the results in those other resources.

The services currently active are:

Check the IMEx site for more information.

What is this view?

Representation of Experimental Features

This section shows the graphical representation of experimental features, where each participant is represented as a white rectangle with a black border and a line for each hundredth amino acid. All available features are attached to their associated participant and their categories are represented in the right side of the legend. The left side of the legend dynamically shows the range statuses occuring in the shown interaction. These are the possible range statuses:

sourceExp

Interacting with the widget

Hover over a feature to see more information in a tooltip.
sourceExp

To display a single interacting region click on it and click again to display all interacting regions.
Displaying all interacting regionsDisplaying one interacting region
sourceExpsourceExp
What is this view?

Dynamic molecular interaction data

This section shows the graphical representation of dynamic molecular interactions. By default it displays all the interactions from one experiment using radio buttons to allow users to highlight interactions in different variable conditions.

Publication

PubMed Id: 17979178

Journal: Proteomics (1615-9853)

Year of Publication: 2007

Title: A novel tandem affinity purification strategy for the efficient isolation and characterisation of native protein complexes.

Author List: Gloeckner CJ., Boldt K., Schumacher A., Roepman R., Ueffing M.

Cross References:

Database
Identifier
Secondary identifier
Qualifier
-
-

Annotations:

Topic
Text
Synapse - Interactions of proteins with an established role in the presynapse.
Apoptosis - Interactions involving proteins with a function related to apoptosis

Experiment (3 interactions)

Accession: EBI-1643967

Name: gloeckner-2007-3

Host organism: Homo sapiens transformed primary embryonal kidney cells

Organism: Homo sapiens transformed primary embryonal kidney cells

Accession: EBI-307628

Name: human-293

Description: Homo sapiens transformed primary embryonal kidney cells

Cross References:

Database
Identifier
Secondary identifier
Qualifier
-
human
-

Interaction Detection Method: tap

Interaction detection method: tap
Accession: EBI-933908

Name: tap

Description: tandem affinity purification

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0676
-
identity
pubmed
11403571
-
primary-reference


Annotations:
Topic
Text
definition
Tandem affinity purification allows rapid purification under native conditions of complexes, even when expressed at their natural level. Prior knowledge of complex composition or function is not required. The TAP method requires fusion of the a multiple tag, either N- or C-terminally, to the target (or bait) protein of interest. The multiple tag allows two steps purification steps ensuring a highly selective complex purification.


Participant Identification Method: ms participant

Participant identification method: ms participant
Accession: EBI-608794

Name: ms participant

Description: Identification by mass spectrometry

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0427
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
identification by mass spectrometry.


Cross References:

Database
Identifier
Secondary identifier
Qualifier
-
-

Annotations:

Topic
Text
marius.ueffing@gsf.de
The authors used a modified TAP tagging protocol. The TAP tag consisted of the Strep tag (WSHPQFEK) and a single FLAG tag. Unlike conventional TAP tagging constructs the TEV cleavage site was ommited.
In this experiment purified proteins were sperated using 4-12% NuPAGE gradient gels (Invitrogen), stained and excised from the gel prior to Mass Spectrophotometric based identification.
Only protein-protein interactions
-
9
imex curation

Interaction

Accession: EBI-1644092

Name: ywha1-ywhaq-tsc2-1

Description: Proteins interacting with 14-3-3 eta

Type: association

Interaction type: association
Accession: EBI-1813191

Name: association

Description: association

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0914
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Interaction between molecules that may participate in formation of one, but possibly more, physical complexes. Often describes a set of molecules that are co-purified in a single pull-down or coimmunoprecipitation but might participate in formation of distinct physical complexes sharing a common bait.


Cross References:

Database
Identifier
Secondary identifier
Qualifier
-
-

Annotations:

Topic
Text
Table S3
The participant EBI-1047183(Q15643) was duplicated. It is posssible that originally this interaction did contain more participants pointing to different Trembl proteins which have been merged to a single swissprot protein later.

Participants (78)

Legend:
A
Annotation and Cross Reference  
P
Experimental Parameter   
S
Stoichiometry   
F
Experimental Feature
C
Participant Confidence
pp1234pp
#
Name
Links
Primary Identifier
Aliases
Description
Species
Expression system
Experimental role
Biological role
Interactor type
More...
pp1234pp
1
EBI-306940
 logo
YWHAH
YWHA1
Protein AS1
14-3-3 protein eta
Homo sapiens
Organism Details

Accession: EBI-874

Name: human

Description: Homo sapiens

Cross References:

Database
Identifier
Secondary identifier
Qualifier
human

Expressed In Details

Accession:

Name:

-
bait
Experimental role: bait
Accession: EBI-49

Name: bait

Description: bait

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0496
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Molecule experimentally treated to capture its interacting partners.


unspecified role
Biological role: unspecified role
Accession: EBI-77781

Name: unspecified role

Description: unspecified role

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0499
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Role not specified or not applicable to the data.


protein
Interactor type: protein
Accession: EBI-619654

Name: protein

Description: protein

Cross References:
Database
Identifier
Secondary identifier
Qualifier
so
SO:0000358
-
see-also
psi-mi
MI:0326
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
A linear polymer of amino acids joined by peptide bonds in a specific sequence.


A
Participant: 14-3-3 protein eta

Participant: 14-3-3 protein eta

Accession: EBI-1644102

Name: EBI-306940

P
Participant: 14-3-3 protein eta

Participant: 14-3-3 protein eta

Accession: EBI-1644102

Name: EBI-306940

Parameters:

Type
Value
Unit
Base
Exponent
Uncertainty
No records found.


S
Participant: 14-3-3 protein eta

Participant: 14-3-3 protein eta

Accession: EBI-1644102

Name: EBI-306940

Stoichiometry: 0.0

F
Participant: 14-3-3 protein eta

Participant: 14-3-3 protein eta

Accession: EBI-1644102

Name: EBI-306940

Features:

Legend:
A
Annotation and Cross Reference  
Feature Name
Feature Type
Detection Method
Range positions
More...
c-terminal
  • c-c
  • A
    Feature: EBI-1644103

    Feature: c-terminal

    Accession: EBI-1644103

    Name: c-terminal

    c-terminal
  • c-c
  • A
    Feature: EBI-1644105

    Feature: c-terminal

    Accession: EBI-1644105

    Name: c-terminal


    C
    Participant: 14-3-3 protein eta

    Participant: 14-3-3 protein eta

    Accession: EBI-1644102

    Name: EBI-306940

    Confidences:

    Type
    Value
    No records found.

    2
    EBI-359832
     logo
    YWHAG
    Protein kinase C inhibitor protein 1
    14-3-3 protein gamma
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: 14-3-3 protein gamma

    Participant: 14-3-3 protein gamma

    Accession: EBI-1644094

    Name: EBI-359832

    P
    Participant: 14-3-3 protein gamma

    Participant: 14-3-3 protein gamma

    Accession: EBI-1644094

    Name: EBI-359832

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: 14-3-3 protein gamma

    Participant: 14-3-3 protein gamma

    Accession: EBI-1644094

    Name: EBI-359832

    Stoichiometry: 0.0

    F
    Participant: 14-3-3 protein gamma

    Participant: 14-3-3 protein gamma

    Accession: EBI-1644094

    Name: EBI-359832

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: 14-3-3 protein gamma

    Participant: 14-3-3 protein gamma

    Accession: EBI-1644094

    Name: EBI-359832

    Confidences:

    Type
    Value
    No records found.

    3
    EBI-1052114
     logo
    La ribonucleoprotein domain family member 1
    LARP1
    KIAA0731

    [+1]
    La-related protein 1
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: La-related protein 1

    Participant: La-related protein 1

    Accession: EBI-1644095

    Name: EBI-1052114

    P
    Participant: La-related protein 1

    Participant: La-related protein 1

    Accession: EBI-1644095

    Name: EBI-1052114

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: La-related protein 1

    Participant: La-related protein 1

    Accession: EBI-1644095

    Name: EBI-1052114

    Stoichiometry: 0.0

    F
    Participant: La-related protein 1

    Participant: La-related protein 1

    Accession: EBI-1644095

    Name: EBI-1052114

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: La-related protein 1

    Participant: La-related protein 1

    Accession: EBI-1644095

    Name: EBI-1052114

    Confidences:

    Type
    Value
    No records found.

    4
    EBI-81968
     logo
    PARD3
    PAR3
    PAR3A

    [+3]
    Partitioning defective 3 homolog
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Partitioning defective 3 homolog

    Participant: Partitioning defective 3 homolog

    Accession: EBI-1644096

    Name: EBI-81968

    P
    Participant: Partitioning defective 3 homolog

    Participant: Partitioning defective 3 homolog

    Accession: EBI-1644096

    Name: EBI-81968

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Partitioning defective 3 homolog

    Participant: Partitioning defective 3 homolog

    Accession: EBI-1644096

    Name: EBI-81968

    Stoichiometry: 0.0

    F
    Participant: Partitioning defective 3 homolog

    Participant: Partitioning defective 3 homolog

    Accession: EBI-1644096

    Name: EBI-81968

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Partitioning defective 3 homolog

    Participant: Partitioning defective 3 homolog

    Accession: EBI-1644096

    Name: EBI-81968

    Confidences:

    Type
    Value
    No records found.

    5
    EBI-359854
     logo
    YWHAQ
    14-3-3 protein tau
    14-3-3 protein T-cell

    [+1]
    14-3-3 protein theta
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: 14-3-3 protein theta

    Participant: 14-3-3 protein theta

    Accession: EBI-1644097

    Name: EBI-359854

    P
    Participant: 14-3-3 protein theta

    Participant: 14-3-3 protein theta

    Accession: EBI-1644097

    Name: EBI-359854

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: 14-3-3 protein theta

    Participant: 14-3-3 protein theta

    Accession: EBI-1644097

    Name: EBI-359854

    Stoichiometry: 0.0

    F
    Participant: 14-3-3 protein theta

    Participant: 14-3-3 protein theta

    Accession: EBI-1644097

    Name: EBI-359854

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: 14-3-3 protein theta

    Participant: 14-3-3 protein theta

    Accession: EBI-1644097

    Name: EBI-359854

    Confidences:

    Type
    Value
    No records found.

    6
    EBI-1051317
     logo
    OSBPL3
    KIAA0704
    ORP3

    [+1]
    Oxysterol-binding protein-related protein 3
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Oxysterol-binding protein-related protein 3

    Participant: Oxysterol-binding protein-related protein 3

    Accession: EBI-1644098

    Name: EBI-1051317

    P
    Participant: Oxysterol-binding protein-related protein 3

    Participant: Oxysterol-binding protein-related protein 3

    Accession: EBI-1644098

    Name: EBI-1051317

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Oxysterol-binding protein-related protein 3

    Participant: Oxysterol-binding protein-related protein 3

    Accession: EBI-1644098

    Name: EBI-1051317

    Stoichiometry: 0.0

    F
    Participant: Oxysterol-binding protein-related protein 3

    Participant: Oxysterol-binding protein-related protein 3

    Accession: EBI-1644098

    Name: EBI-1051317

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Oxysterol-binding protein-related protein 3

    Participant: Oxysterol-binding protein-related protein 3

    Accession: EBI-1644098

    Name: EBI-1051317

    Confidences:

    Type
    Value
    No records found.

    7
    EBI-721019
     logo
    KLC1
    KLC
    KNS2
    Kinesin light chain 1
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Kinesin light chain 1

    Participant: Kinesin light chain 1

    Accession: EBI-1644099

    Name: EBI-721019

    P
    Participant: Kinesin light chain 1

    Participant: Kinesin light chain 1

    Accession: EBI-1644099

    Name: EBI-721019

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Kinesin light chain 1

    Participant: Kinesin light chain 1

    Accession: EBI-1644099

    Name: EBI-721019

    Stoichiometry: 0.0

    F
    Participant: Kinesin light chain 1

    Participant: Kinesin light chain 1

    Accession: EBI-1644099

    Name: EBI-721019

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Kinesin light chain 1

    Participant: Kinesin light chain 1

    Accession: EBI-1644099

    Name: EBI-721019

    Confidences:

    Type
    Value
    No records found.

    8
    EBI-347088
     logo
    YWHAZ
    Protein kinase C inhibitor protein 1
    14-3-3 protein zeta/delta
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: 14-3-3 protein zeta/delta

    Participant: 14-3-3 protein zeta/delta

    Accession: EBI-1644100

    Name: EBI-347088

    P
    Participant: 14-3-3 protein zeta/delta

    Participant: 14-3-3 protein zeta/delta

    Accession: EBI-1644100

    Name: EBI-347088

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: 14-3-3 protein zeta/delta

    Participant: 14-3-3 protein zeta/delta

    Accession: EBI-1644100

    Name: EBI-347088

    Stoichiometry: 0.0

    F
    Participant: 14-3-3 protein zeta/delta

    Participant: 14-3-3 protein zeta/delta

    Accession: EBI-1644100

    Name: EBI-347088

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: 14-3-3 protein zeta/delta

    Participant: 14-3-3 protein zeta/delta

    Accession: EBI-1644100

    Name: EBI-347088

    Confidences:

    Type
    Value
    No records found.

    9
    EBI-713738
     logo
    p21-activated kinase 4
    PAK4
    KIAA1142
    Serine/threonine-protein kinase PAK 4
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Serine/threonine-protein kinase PAK 4

    Participant: Serine/threonine-protein kinase PAK 4

    Accession: EBI-1644101

    Name: EBI-713738

    P
    Participant: Serine/threonine-protein kinase PAK 4

    Participant: Serine/threonine-protein kinase PAK 4

    Accession: EBI-1644101

    Name: EBI-713738

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Serine/threonine-protein kinase PAK 4

    Participant: Serine/threonine-protein kinase PAK 4

    Accession: EBI-1644101

    Name: EBI-713738

    Stoichiometry: 0.0

    F
    Participant: Serine/threonine-protein kinase PAK 4

    Participant: Serine/threonine-protein kinase PAK 4

    Accession: EBI-1644101

    Name: EBI-713738

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Serine/threonine-protein kinase PAK 4

    Participant: Serine/threonine-protein kinase PAK 4

    Accession: EBI-1644101

    Name: EBI-713738

    Confidences:

    Type
    Value
    No records found.

    10
    EBI-1049582
     logo
    IRS2
    Insulin receptor substrate 2
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Insulin receptor substrate 2

    Participant: Insulin receptor substrate 2

    Accession: EBI-1644107

    Name: EBI-1049582

    P
    Participant: Insulin receptor substrate 2

    Participant: Insulin receptor substrate 2

    Accession: EBI-1644107

    Name: EBI-1049582

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Insulin receptor substrate 2

    Participant: Insulin receptor substrate 2

    Accession: EBI-1644107

    Name: EBI-1049582

    Stoichiometry: 0.0

    F
    Participant: Insulin receptor substrate 2

    Participant: Insulin receptor substrate 2

    Accession: EBI-1644107

    Name: EBI-1049582

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Insulin receptor substrate 2

    Participant: Insulin receptor substrate 2

    Accession: EBI-1644107

    Name: EBI-1049582

    Confidences:

    Type
    Value
    No records found.

    11
    EBI-1049676
     logo
    NRip11
    RAB11FIP2
    KIAA0941
    Rab11 family-interacting protein 2
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Rab11 family-interacting protein 2

    Participant: Rab11 family-interacting protein 2

    Accession: EBI-1644108

    Name: EBI-1049676

    P
    Participant: Rab11 family-interacting protein 2

    Participant: Rab11 family-interacting protein 2

    Accession: EBI-1644108

    Name: EBI-1049676

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Rab11 family-interacting protein 2

    Participant: Rab11 family-interacting protein 2

    Accession: EBI-1644108

    Name: EBI-1049676

    Stoichiometry: 0.0

    F
    Participant: Rab11 family-interacting protein 2

    Participant: Rab11 family-interacting protein 2

    Accession: EBI-1644108

    Name: EBI-1049676

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Rab11 family-interacting protein 2

    Participant: Rab11 family-interacting protein 2

    Accession: EBI-1644108

    Name: EBI-1049676

    Confidences:

    Type
    Value
    No records found.

    12
    EBI-1644036
     logo
    TBC1D1
    KIAA1108
    TBC1 domain family member 1
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: TBC1 domain family member 1

    Participant: TBC1 domain family member 1

    Accession: EBI-1644109

    Name: EBI-1644036

    P
    Participant: TBC1 domain family member 1

    Participant: TBC1 domain family member 1

    Accession: EBI-1644109

    Name: EBI-1644036

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: TBC1 domain family member 1

    Participant: TBC1 domain family member 1

    Accession: EBI-1644109

    Name: EBI-1644036

    Stoichiometry: 0.0

    F
    Participant: TBC1 domain family member 1

    Participant: TBC1 domain family member 1

    Accession: EBI-1644109

    Name: EBI-1644036

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: TBC1 domain family member 1

    Participant: TBC1 domain family member 1

    Accession: EBI-1644109

    Name: EBI-1644036

    Confidences:

    Type
    Value
    No records found.

    13
    EBI-359815
     logo
    YWHAB
    Protein kinase C inhibitor protein 1
    Protein 1054
    14-3-3 protein beta/alpha
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.