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What is this view?

Binary interactions

In this tab, we display the list of interactions that you have selected using one of our search features. Despite the fact that our data are annotated to accurately reflect the interactions reported in scientific literature, the data is shown in this view as binary interactions. Whenever the data was reported as a co-complex involving more than two molecules, we store it as such in the IntAct database and post-process it so the portal can show it as binary interaction. This post-processing is the Spoke Expansion model (connects bait to all preys):



sourceExp

At any moment you can choose to display the expansion column in this view in order to see which interaction are spoke expanded and which are not.

Description of what has changed

  • We have added more download options to allow users to retrieve their interaction set using more standard formats such as PSI-MI XML and PSIMITAB (version 2.5, 2.6 or 2.7) but also XGMML, RDF and Biopax (level 2 and 3).
  • We have now four different table views : minimal(molecule names and interaction AC), basic (minimal + molecule links, interaction detection method, negative), standard(minimal + molecule species, confidences, publication details, experiment details), expanded (standard + more experiment details) and complete (all mitab 2.7 columns).

Configuring the view to your need"

In the header of the interaction table you will find a button: ‘Change Column Display’ that will show you all the columns/Table views available and allow you to update the current selected set.

Downloading the data into Standard formats"

In the header of the interaction table you will find a drop down list that contains all the formats currently supported when downloading the interaction data. Select one of them and click the export button next to the list. Please note that PSI-MI XML is only available when the interaction set is no bigger than 1000 interactions.

Opening the interaction details"

Clicking on the magnifying glass in the first column of the interaction table will open the details of the corresponding interaction in the Interaction Details tab, giving you access to more details of the manually curated record.

What is this view?

Browsing (Browse Tab)

This tab is meant to give you access to more content based on the currently selected set of interactions. Please note that linking to third party resources will only include up to 200 molecules , if you exceed this number you will see the warning icon (This number has been reduced to 125 molecules for mRNA expression). Now let’s look at the features available to you:

Limiting the scope of the current dataset with the Uniprot Taxonomy ontology

Allows users to browse the Uniprot Taxonomy hierarchy as a tree and select terms in order to narrow down their dataset. Once a term is selected, you are taken back to the interaction tab to review your dataset.

Limiting the scope of the current dataset with the GO ontology

Allows users to browse the GO hierarchy as a tree and select terms in order to narrow down their dataset. Once a term is selected, you are taken back to the interaction tab to review your dataset.

Limiting the scope of the current dataset with the ChEBI ontology

Allows users to browse the ChEBI hierarchy as a tree and select terms in order to narrow down their dataset. Once a term is selected, you are taken back to the interaction tab to review your dataset.

Bulk linking to third party resources by using involved proteins

  • Proteins by Reactome pathway: Sends your proteins to the Reactome SkyPainter that will show you the pathways in which these molecules are know to play a role.
  • Proteins by Chromosomal location: Sends your list of proteins to Ensembl’s Karyotype viewer and overlays the proteins on the chromosomes.
  • Proteins by mRNA expression: Sends your set of proteins to the ArrayExpress Atlas that will show the known gene expression based on experimental studies.
What is this view?

Searching Interactions (Search Tab)

As you can see in this tab we are now trying to give you more targeted choice to do your queries, please note that the examples provided in this tab are live links so you can simply click them to see the resulting interactions sets.

Using the Quick Search

In this search panel you are free to type anything that might relate to interactions, whether it is properties of their interactor (gene name, identifiers, GO term…) or more specific to the interaction like publication, authors, experimental detection method, ...

Some examples:

  • Try the query: imatinib
    This is a drug for which we have curated a number of interactions.
    Once you press the search button you should be taken to the Interaction Tab that lists 130 binary interactions.
    If you want to construct more complex queries we recommend you take a look at the Molecular Interaction Query Language, accessible from the quick search panel.
  • Try the query: species:yeast AND type:"direct interaction"
    This query selects all interactions involving yeast interactors that have been shown to have direct interactions. If you customize the column display of the interaction tab, you will see that not only “direct interaction” have been selected but also children terms in the PSI-MI ontology.

Using the Ontology Search

Open the Rearch Tab. This panel is specialised to give you an easy access to ontology search. So far you can search on 4 ontologies:

  • Gene Ontology
  • InterPro
  • PSI-MI
  • ChEBI

Whenever you start typing a query in this search panel, the system will search as you type and propose a list of matching controlled vocabulary terms. You can then select one of them and select matching interactions.

For example, type: cancer
You will be presented with a few choices, please note that each term is followed by the count of matching interactions in the IntAct database.

Select a term with the mouse or using the keyboard cursor keys and you will be taken to the interaction tab.

Searching the Compound chemical structure

In this panel you will be able to draw all or part of a chemical structure and search for chemical compounds. If you get any matched, you can then see all interactions involving them.

First you have to open up the chemical search panel so that the applet can load, it might take a few seconds. Then you can start drawing your structure, for instance:

Once you have drawn your structure, select Similarity and press Search. You should be presented with a list of matching compound. Now choose one molecule and click the link: IntAct interactions. You will be taken to the interaction tab to review the data.

Complex Expansion

Binary interactions generated by co-complex expansion

Why should you care about complex expansion ?

Some experimental methods such as Tandem Affinity Purification do generate molecular interactions that can involve more than 2 molecules. Despite the fact that IntAct curation team do capture the molecular interaction as they were reported in the corresponding experiment, when you search using the intact web site, the results of your query is always shown as set of binary interactions (i.e. 2 molecules). We would like to draw your attention on the fact that whenever the reported interaction was a co-complex we do apply an expansion algorithm that transform this n-ary interaction into a set of binary interactions. While none of these agorithms is perfect and will very likely generate some false positive interactions, it is useful to present the data in a consistent manner. Bear in mind that we will strive to differentiate in the search results which interactions are a real experimental binary from expanded ones.

Existing expansion algorithm

There are several known algorithm allowing to transform an n-ary interaction into a set of binaries. The illustration below present the two well known expansion model and illustrates why they can be incorrect.



sourceExp

  • Spoke expansion: Links the bait molecule to all prey molecules. If N is the count of molecule in the complex, it generated N-1 binary interactions.
  • Matrix expansion: Links all molecule to all other molecule present in the complex. If N is the count of molecule in the complex, it generated (N*(N-1))/2 binary interactions.

Now the issue (as illustrated at the bottom right of the diagram above) with these two models lies in the fact that the real complex might not be articulated around the experimental bait but instead, this bait might be linked to a smaller complex, hence most binary interaction generated by spoke and matrix expansion result in false positive.



PSICQUIC

How is the number of interactions in other databases obtained?

PSICQUIC is a standard way to access molecular interaction databases across which it repeats the same query. The number of databases providing data may vary, depending on the status of their services and only those that are active are used in this query. By clicking on the number of interactions you will be redirected to the PSICQUIC View, where you can browse the results in those other resources.

The services currently active are:

Check the PSICQUIC site for more information.

IMEx

What is the significance of the IMEx dataset?"

IMEx is a network of databases which have agreed to supply a non-redundant set of data expertly manually annotated to the same consistent detailed standard which, as such, represents a high-quality subset of the data each individually provides. The number of databases providing data may vary, depending on the status of their services and only those that are active are used in this query. By clicking on the number of interactions you will be redirected to the IMEx View, where you can browse the results in those other resources.

The services currently active are:

Check the IMEx site for more information.

What is this view?

Representation of Experimental Features

This section shows the graphical representation of experimental features, where each participant is represented as a white rectangle with a black border and a line for each hundredth amino acid. All available features are attached to their associated participant and their categories are represented in the right side of the legend. The left side of the legend dynamically shows the range statuses occuring in the shown interaction. These are the possible range statuses:

sourceExp

Interacting with the widget

Hover over a feature to see more information in a tooltip.
sourceExp

To display a single interacting region click on it and click again to display all interacting regions.
Displaying all interacting regionsDisplaying one interacting region
sourceExpsourceExp
What is this view?

Dynamic molecular interaction data

This section shows the graphical representation of dynamic molecular interactions. By default it displays all the interactions from one experiment using radio buttons to allow users to highlight interactions in different variable conditions.

Publication

PubMed Id: 26496610

Journal: Cell

Year of Publication: 2015

Title: A human interactome in three quantitative dimensions organized by stoichiometries and abundances.

Author List: Hein MY., Hubner NC., Poser I., Cox J., Nagaraj N., Toyoda Y., Gak IA., Weisswange I., Mansfeld J., Buchholz F., Hyman AA., Mann M.

Cross References:

Experiment (1323 interactions)

Accession: EBI-10990999

Name: hein-2015-2

Host organism: Homo sapiens epitheloid cervix carcinoma cells

Organism: Homo sapiens epitheloid cervix carcinoma cells

Accession: EBI-307614

Name: human-hela

Description: Homo sapiens epitheloid cervix carcinoma cells

Cross References:

Database
Identifier
Secondary identifier
Qualifier
human
-

Interaction Detection Method: anti tag coip

Interaction detection method: anti tag coip
Accession: EBI-90

Name: anti tag coip

Description: anti tag coimmunoprecipitation

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0007
-
identity
pubmed
7708014
-
primary-reference


Annotations:
Topic
Text
definition
A specific antibody for the molecule of interest is not available, therefore the bait protein is expressed as a hybrid protein fused to a tag peptide/protein for which efficient and specific antibodies or a specific ligand are available.


Participant Identification Method: sequence tag

Participant identification method: sequence tag
Accession: EBI-81

Name: sequence tag

Description: sequence tag identification

Cross References:
Database
Identifier
Secondary identifier
Qualifier
pubmed
11752590
-
primary-reference
pubmed
11805837
-
primary-reference
psi-mi
MI:0102
-
identity
pubmed
10967324
-
primary-reference


Annotations:
Topic
Text
definition
This approach leads to protein identification by combining mass measurement and short amino acid sequence information obtained by tandem mass spectrometry. This information is then used to automatically find the best match in a sequence database. A mixture of peptides derived from a protease digestion is analysed by nanoelectrospray LC-MS/MS (Liquid Chromatography Tandem Mass Spectrometer or nanoESI MS/MS) mass spectrometry. Electrospray mass spectrometry cannot be applied to dilute samples and is affected by high salt. As a consequence peptides, normally extracted from acrylamide gels by in situ proteolysis, are desalted and concentrated on a microcolumn followed by elution into a capillary used for nanoelectrospray tandem mass spectrometry. A first mass spectrum (Normal mass spectrum or Q1 mass spectrum) gives information about the masses of all the peptides. Peptides observed in the normal mass spectrum are isolated in turn and dissociated into fragments by collision with gas molecules within the mass spectrometer. Some of the fragments obtained from a peptide constitute a nested set, differing by one amino acid, and the mass difference between them allows assignment of a partial sequence. The masses of the fragments define the position of the partial sequence in the peptide. Together with the cleavage specificity of the protease used to cleave the protein, and mass information such sequence tag provides much higher search specificity to match the a database entry. The procedure is repeated with several peptides from the digest, resulting in multiple identifications of the same protein or identification of several proteins from the peptide mixture. Unknown proteins can easily be identified by using the high specificity of the peptide sequence tag for searches in most sequence databases including EST or genome databases.


Cross References:

Annotations:

Topic
Text
Author confidence calculation Protein identifications as obtained from MS spectra processing were filtered, removing hits to the reverse decoy database as well as proteins only identified by modified peptides. It was required that each protein be quantified in all replicates from the AP-MS samples of at least one cell line. Protein LFQ intensities were logarithmized and missing values imputed by values simulating noise around the detection limit. For each protein, a non-parametric method was used to select a subset of samples that provide a distribution of background intensities for this protein. This subset was used first to normalize all protein intensities to represent relative enrichment, and then to serve as the control group for a two-tailed Welch’s t test. Specific outliers in the volcano plots of logarithmized p values against enrichments were determined by an approach making use of the asymmetry in the outlier population (see url links for each interaction). Two cut-offs of different stringencies, representing 1 and 5% of enrichment false discovery rate (FDR), respectively, were used. Correlation coefficients between the intensity profiles of interacting proteins were calculated as additional quality parameters. Enrichment FDR (classes A, B and C) and profile correlation (modifier + or –) define the confidence class of an interaction (see author assigned scores). Relative abundance and interaction stoichiometry estimations: Estimating interaction stoichiometries requires the comparison of the amounts of different proteins relative to each other in one IP. To this end, the authors first subtracted the median intensity across all samples to account for the proportion due to unspecific binding. Then LFQ intensities were divided by the number of theoretically observable peptides for this protein. This corrects for biases introduced by different lengths of the protein sequences and the frequency and distribution of proteolytic cleavage sites. Finally, interaction stoichiometries were expressed relative to the bait protein. Cellular copy numbers and relative abundances were calculated using a similar approach on the whole proteome data and brought to absolute scale by normalization to a total protein amount of 200 pg in a cell volume of 1 pl for a HeLa cell.
imex curation
hein@biochem.mpg.de,mmann@biochem.mpg.de
09-06-2015: Submitted by Marco Hein, Max Planck Institute of Biochemistry, Martinsried.
Only protein-protein interactions
-
PAM-SILAC experiments mentioned in the text and summarized in figure 5 were not added since the authors stated that the SILAC data just confirmed the large screening results for a set of selected baits and did not provide further detail on the interactions. The cell lines tested with PAM-SILAC were MCP_ky_0002211 (interaction id EBI-11003881), MCP_ky_0006158 (interaction id EBI-11131339) and MCP_ky_0003099 (interaction id EBI-11029579).

Interaction

Accession: EBI-11132267

Name: cdk4-aspm-1

Description: -

Type: association

Interaction type: association
Accession: EBI-1813191

Name: association

Description: association

Cross References:
Database
Identifier
Secondary identifier
Qualifier
pubmed
14755292
-
primary-reference
psi-mi
MI:0914
-
identity


Annotations:
Topic
Text
definition
Interaction between molecules that may participate in formation of one, but possibly more, physical complexes. Often describes a set of molecules that are co-purified in a single pull-down or coimmunoprecipitation but might participate in formation of distinct physical complexes sharing a common bait.


Cross References:

Database
Identifier
Secondary identifier
Qualifier
-

Annotations:

Topic
Text
Suppl. table S2. For bait details see supp. table S1
Cell line id: MCP_ky_0006201

Participants (12)

Legend:
A
Annotation and Cross Reference  
P
Experimental Parameter   
S
Stoichiometry   
F
Experimental Feature
C
Participant Confidence
#
Name
Links
Primary Identifier
Aliases
Description
Species
Expression system
Experimental role
Biological role
Interactor type
More...
1
EBI-295644
 logo
CDK4
Cell division protein kinase 4
PSK-J3
Cyclin-dependent kinase 4
Homo sapiens
Organism Details

Accession: EBI-874

Name: human

Description: Homo sapiens

Cross References:

Database
Identifier
Secondary identifier
Qualifier
human

Expressed In Details

Accession:

Name:

-
bait
Experimental role: bait
Accession: EBI-49

Name: bait

Description: bait

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0496
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Molecule experimentally treated to capture its interacting partners.


unspecified role
Biological role: unspecified role
Accession: EBI-77781

Name: unspecified role

Description: unspecified role

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0499
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Role not specified or not applicable to the data.


protein
Interactor type: protein
Accession: EBI-619654

Name: protein

Description: protein

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0326
-
identity
pubmed
14755292
-
primary-reference
so
SO:0000358
-
see-also


Annotations:
Topic
Text
definition
A linear polymer of amino acids joined by peptide bonds in a specific sequence.


A
Participant: Cyclin-dependent kinase 4

Participant: Cyclin-dependent kinase 4

Accession: EBI-11132292

Name: EBI-295644

Cross References:

Database
Identifier
Secondary identifier
Qualifier
-

P
Participant: Cyclin-dependent kinase 4

Participant: Cyclin-dependent kinase 4

Accession: EBI-11132292

Name: EBI-295644

Parameters:

Type
Value
Unit
Base
Exponent
Uncertainty
No records found.


S
Participant: Cyclin-dependent kinase 4

Participant: Cyclin-dependent kinase 4

Accession: EBI-11132292

Name: EBI-295644

Stoichiometry: 0.0

F
Participant: Cyclin-dependent kinase 4

Participant: Cyclin-dependent kinase 4

Accession: EBI-11132292

Name: EBI-295644

Features:

Legend:
A
Annotation and Cross Reference  
Feature Name
Feature Type
Detection Method
Range positions
More...
lap tag
  • c-c
  • A
    Feature: EBI-11132345

    Feature: lap tag

    Accession: EBI-11132345

    Name: lap tag

    Annotations:

    Topic
    Text
    This tag was formed by the concatentation of the following sub-sequences: TEV cleavage site,S epitope,PreScission protease cleavage site,GFP


    C
    Participant: Cyclin-dependent kinase 4

    Participant: Cyclin-dependent kinase 4

    Accession: EBI-11132292

    Name: EBI-295644

    Confidences:

    Type
    Value
    No records found.

    2
    EBI-375053
     logo
    CDKN2A
    CDKN2
    Cyclin-dependent kinase 4 inhibitor A

    [+3]
    Cyclin-dependent kinase inhibitor 2A
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference
    so
    SO:0000358
    -
    see-also


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Cyclin-dependent kinase inhibitor 2A

    Participant: Cyclin-dependent kinase inhibitor 2A

    Accession: EBI-11132287

    Name: EBI-375053

    Annotations:

    P
    Participant: Cyclin-dependent kinase inhibitor 2A

    Participant: Cyclin-dependent kinase inhibitor 2A

    Accession: EBI-11132287

    Name: EBI-375053

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    1.561
    10
    0
    0.0
    9.782
    10
    0
    0.0


    S
    Participant: Cyclin-dependent kinase inhibitor 2A

    Participant: Cyclin-dependent kinase inhibitor 2A

    Accession: EBI-11132287

    Name: EBI-375053

    Stoichiometry: 0.0

    F
    Participant: Cyclin-dependent kinase inhibitor 2A

    Participant: Cyclin-dependent kinase inhibitor 2A

    Accession: EBI-11132287

    Name: EBI-375053

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Cyclin-dependent kinase inhibitor 2A

    Participant: Cyclin-dependent kinase inhibitor 2A

    Accession: EBI-11132287

    Name: EBI-375053

    Confidences:

    Type
    Value
    A+

    3
    EBI-295695
     logo
    NUP133
    Nucleoporin Nup133
    133 kDa nucleoporin
    Nuclear pore complex protein Nup133
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference
    so
    SO:0000358
    -
    see-also


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Nuclear pore complex protein Nup133

    Participant: Nuclear pore complex protein Nup133

    Accession: EBI-11132295

    Name: EBI-295695

    Annotations:

    P
    Participant: Nuclear pore complex protein Nup133

    Participant: Nuclear pore complex protein Nup133

    Accession: EBI-11132295

    Name: EBI-295695

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    3.72
    10
    -5
    0.0
    7.106
    10
    0
    0.0


    S
    Participant: Nuclear pore complex protein Nup133

    Participant: Nuclear pore complex protein Nup133

    Accession: EBI-11132295

    Name: EBI-295695

    Stoichiometry: 0.0

    F
    Participant: Nuclear pore complex protein Nup133

    Participant: Nuclear pore complex protein Nup133

    Accession: EBI-11132295

    Name: EBI-295695

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Nuclear pore complex protein Nup133

    Participant: Nuclear pore complex protein Nup133

    Accession: EBI-11132295

    Name: EBI-295695

    Confidences:

    Type
    Value
    B-

    4
    EBI-10963884
     logo
    TMEM126A
    Transmembrane protein 126A
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference
    so
    SO:0000358
    -
    see-also


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Transmembrane protein 126A

    Participant: Transmembrane protein 126A

    Accession: EBI-11132300

    Name: EBI-10963884

    Annotations:

    P
    Participant: Transmembrane protein 126A

    Participant: Transmembrane protein 126A

    Accession: EBI-11132300

    Name: EBI-10963884

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    2.251
    10
    -4
    0.0
    1.79
    10
    0
    0.0


    S
    Participant: Transmembrane protein 126A

    Participant: Transmembrane protein 126A

    Accession: EBI-11132300

    Name: EBI-10963884

    Stoichiometry: 0.0

    F
    Participant: Transmembrane protein 126A

    Participant: Transmembrane protein 126A

    Accession: EBI-11132300

    Name: EBI-10963884

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Transmembrane protein 126A

    Participant: Transmembrane protein 126A

    Accession: EBI-11132300

    Name: EBI-10963884

    Confidences:

    Type
    Value
    B-

    5
    EBI-10963388
     logo
    ZC3H14
    Mammalian suppressor of tau pathology-2
    Renal carcinoma antigen NY-REN-37
    Zinc finger CCCH domain-containing protein 14
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference
    so
    SO:0000358
    -
    see-also


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Zinc finger CCCH domain-containing protein 14

    Participant: Zinc finger CCCH domain-containing protein 14

    Accession: EBI-11132305

    Name: EBI-10963388

    Annotations:

    Topic
    Text
    Missing cellular copy number precludes calculation of relative abundance.
    B-

    P
    Participant: Zinc finger CCCH domain-containing protein 14

    Participant: Zinc finger CCCH domain-containing protein 14

    Accession: EBI-11132305

    Name: EBI-10963388

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    3.481
    10
    -5
    0.0


    S
    Participant: Zinc finger CCCH domain-containing protein 14

    Participant: Zinc finger CCCH domain-containing protein 14

    Accession: EBI-11132305

    Name: EBI-10963388

    Stoichiometry: 0.0

    F
    Participant: Zinc finger CCCH domain-containing protein 14

    Participant: Zinc finger CCCH domain-containing protein 14

    Accession: EBI-11132305

    Name: EBI-10963388

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Zinc finger CCCH domain-containing protein 14

    Participant: Zinc finger CCCH domain-containing protein 14

    Accession: EBI-11132305

    Name: EBI-10963388

    Confidences:

    Type
    Value
    B-

    6
    EBI-968626
     logo
    CDK13
    CDC2L
    CHED

    [+6]
    Cyclin-dependent kinase 13
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference
    so
    SO:0000358
    -
    see-also


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Cyclin-dependent kinase 13

    Participant: Cyclin-dependent kinase 13

    Accession: EBI-11132310

    Name: EBI-968626

    Annotations:

    P
    Participant: Cyclin-dependent kinase 13

    Participant: Cyclin-dependent kinase 13

    Accession: EBI-11132310

    Name: EBI-968626

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    1.799
    10
    -2
    0.0
    5.584
    10
    -1
    0.0


    S
    Participant: Cyclin-dependent kinase 13

    Participant: Cyclin-dependent kinase 13

    Accession: EBI-11132310

    Name: EBI-968626

    Stoichiometry: 0.0

    F
    Participant: Cyclin-dependent kinase 13

    Participant: Cyclin-dependent kinase 13

    Accession: EBI-11132310

    Name: EBI-968626

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Cyclin-dependent kinase 13

    Participant: Cyclin-dependent kinase 13

    Accession: EBI-11132310

    Name: EBI-968626

    Confidences:

    Type
    Value
    A

    7
    EBI-308745
     logo
    ZNFX1
    KIAA1404
    NFX1-type zinc finger-containing protein 1
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference
    so
    SO:0000358
    -
    see-also


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: NFX1-type zinc finger-containing protein 1

    Participant: NFX1-type zinc finger-containing protein 1

    Accession: EBI-11132315

    Name: EBI-308745

    Annotations:

    P
    Participant: NFX1-type zinc finger-containing protein 1

    Participant: NFX1-type zinc finger-containing protein 1

    Accession: EBI-11132315

    Name: EBI-308745

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    3.285
    10
    -4
    0.0
    3.716
    10
    -1
    0.0


    S
    Participant: NFX1-type zinc finger-containing protein 1

    Participant: NFX1-type zinc finger-containing protein 1

    Accession: EBI-11132315

    Name: EBI-308745

    Stoichiometry: 0.0

    F
    Participant: NFX1-type zinc finger-containing protein 1

    Participant: NFX1-type zinc finger-containing protein 1

    Accession: EBI-11132315

    Name: EBI-308745

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: NFX1-type zinc finger-containing protein 1

    Participant: NFX1-type zinc finger-containing protein 1

    Accession: EBI-11132315

    Name: EBI-308745

    Confidences:

    Type
    Value
    B-

    8
    EBI-11100581
     logo
    SMTN
    SMSMO
    L2
    Smoothelin
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference
    so
    SO:0000358
    -
    see-also


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Smoothelin

    Participant: Smoothelin

    Accession: EBI-11132320

    Name: EBI-11100581

    Annotations:

    P
    Participant: Smoothelin

    Participant: Smoothelin

    Accession: EBI-11132320

    Name: EBI-11100581

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    8.448
    10
    -5
    0.0
    5.114
    10
    -2
    0.0


    S
    Participant: Smoothelin

    Participant: Smoothelin

    Accession: EBI-11132320

    Name: EBI-11100581

    Stoichiometry: 0.0

    F
    Participant: Smoothelin

    Participant: Smoothelin

    Accession: EBI-11132320

    Name: EBI-11100581

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Smoothelin

    Participant: Smoothelin

    Accession: EBI-11132320

    Name: EBI-11100581

    Confidences:

    Type
    Value
    B

    9
    EBI-11132271
     logo
    SORBS1
    KIAA0894
    KIAA1296

    [+5]
    Sorbin and SH3 domain-containing protein 1
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference
    so
    SO:0000358
    -
    see-also


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Sorbin and SH3 domain-containing protein 1

    Participant: Sorbin and SH3 domain-containing protein 1

    Accession: EBI-11132325

    Name: EBI-11132271

    Annotations:

    P
    Participant: Sorbin and SH3 domain-containing protein 1

    Participant: Sorbin and SH3 domain-containing protein 1

    Accession: EBI-11132325

    Name: EBI-11132271

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    4.001
    10
    -5
    0.0
    2.882
    10
    -2
    0.0


    S
    Participant: Sorbin and SH3 domain-containing protein 1

    Participant: Sorbin and SH3 domain-containing protein 1

    Accession: EBI-11132325

    Name: EBI-11132271

    Stoichiometry: 0.0

    F
    Participant: Sorbin and SH3 domain-containing protein 1

    Participant: Sorbin and SH3 domain-containing protein 1

    Accession: EBI-11132325

    Name: EBI-11132271

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Sorbin and SH3 domain-containing protein 1

    Participant: Sorbin and SH3 domain-containing protein 1

    Accession: EBI-11132325

    Name: EBI-11132271

    Confidences:

    Type
    Value
    B-

    10
    EBI-375013
     logo
    CCND3
    G1/S-specific cyclin-D3
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference
    so
    SO:0000358
    -
    see-also


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: G1/S-specific cyclin-D3

    Participant: G1/S-specific cyclin-D3

    Accession: EBI-11132330

    Name: EBI-375013

    Annotations:

    Topic
    Text
    Missing cellular copy number precludes calculation of relative abundance.
    A

    P
    Participant: G1/S-specific cyclin-D3

    Participant: G1/S-specific cyclin-D3

    Accession: EBI-11132330

    Name: EBI-375013

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    8.798
    10
    -4
    0.0


    S
    Participant: G1/S-specific cyclin-D3

    Participant: G1/S-specific cyclin-D3

    Accession: EBI-11132330

    Name: EBI-375013

    Stoichiometry: 0.0

    F
    Participant: G1/S-specific cyclin-D3

    Participant: G1/S-specific cyclin-D3

    Accession: EBI-11132330

    Name: EBI-375013

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: G1/S-specific cyclin-D3

    Participant: G1/S-specific cyclin-D3

    Accession: EBI-11132330

    Name: EBI-375013

    Confidences:

    Type
    Value
    A

    11
    EBI-1057621
     logo
    ASPM
    MCPH5
    Abnormal spindle protein homolog
    Abnormal spindle-like microcephaly-associated protein
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference
    so
    SO:0000358
    -
    see-also


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Abnormal spindle-like microcephaly-associated protein

    Participant: Abnormal spindle-like microcephaly-associated protein

    Accession: EBI-11132335

    Name: EBI-1057621

    Annotations:

    P
    Participant: Abnormal spindle-like microcephaly-associated protein

    Participant: Abnormal spindle-like microcephaly-associated protein

    Accession: EBI-11132335

    Name: EBI-1057621

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    6.287
    10
    -5
    0.0
    2.315
    10
    -1
    0.0


    S
    Participant: Abnormal spindle-like microcephaly-associated protein

    Participant: Abnormal spindle-like microcephaly-associated protein

    Accession: EBI-11132335

    Name: EBI-1057621

    Stoichiometry: 0.0

    F
    Participant: Abnormal spindle-like microcephaly-associated protein

    Participant: Abnormal spindle-like microcephaly-associated protein

    Accession: EBI-11132335

    Name: EBI-1057621

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Abnormal spindle-like microcephaly-associated protein

    Participant: Abnormal spindle-like microcephaly-associated protein

    Accession: EBI-11132335

    Name: EBI-1057621

    Confidences:

    Type
    Value
    B-

    12
    EBI-711280
     logo
    CDKN2B
    MTS2
    p14-INK4b

    [+2]
    Cyclin-dependent kinase 4 inhibitor B
    Homo sapiens
    Organism Details

    Accession: EBI-874

    Name: human

    Description: Homo sapiens

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    human

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference
    so
    SO:0000358
    -
    see-also


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Cyclin-dependent kinase 4 inhibitor B

    Participant: Cyclin-dependent kinase 4 inhibitor B

    Accession: EBI-11132340

    Name: EBI-711280

    Annotations:

    Topic
    Text
    Missing cellular copy number precludes calculation of relative abundance.
    A+

    P
    Participant: Cyclin-dependent kinase 4 inhibitor B

    Participant: Cyclin-dependent kinase 4 inhibitor B

    Accession: EBI-11132340

    Name: EBI-711280

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    8.495
    10
    -4
    0.0


    S
    Participant: Cyclin-dependent kinase 4 inhibitor B

    Participant: Cyclin-dependent kinase 4 inhibitor B

    Accession: EBI-11132340

    Name: EBI-711280

    Stoichiometry: 0.0

    F
    Participant: Cyclin-dependent kinase 4 inhibitor B

    Participant: Cyclin-dependent kinase 4 inhibitor B

    Accession: EBI-11132340

    Name: EBI-711280

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Cyclin-dependent kinase 4 inhibitor B

    Participant: Cyclin-dependent kinase 4 inhibitor B

    Accession: EBI-11132340

    Name: EBI-711280

    Confidences:

    Type
    Value
    A+


    Graphical Representation of Experimental Features