{"systematicName":"CPA1:CPA2","synonyms":["carbamoyl-phosphate synthase (glutamine-hydrolysing)","carbamoyl-phosphate synthetase (glutamine-hydrolysing)","carbamoylphosphate synthetase II","carbamyl phosphate synthetase (glutamine)","glutamine-dependent carbamyl phosphate synthetase","GD-CPSase","glutamine-dependent carbamoyl-phosphate synthase","carbamoyl phosphate synthetase","CPS","CPS-A","carbon-dioxide::L-glutamine amido-ligase (ADP-forming, carbamate-phosphorylating)","CPSase A","arginine pathway carbamoylphosphate synthase","carbamoyl-phosphate synthase arginine-specific","hydrogen-carbonate:L-glutamine amido-ligase (ADP-forming, carbamate-phosphorylating)","CARA-CARB complex"],"functions":["Carbamoyl-phosphate synthase is a heterodimer consisting in the CPA1 and CPA2 subunits. CPS catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, as the first step in biosynthetic pathway leading to formation of arginine and/or urea. The small subunit (glutamine amidotransferase) binds and cleaves glutamine and the large subunit (synthetase) accepts the ammonia moiety cleaved from glutamine, binds all of the remaining substrates, and carries out all of the other catalytic events."],"properties":["MW =140, 000 (7.9S) (estimated by sucrose gradient centrifugation); MW = 175, 000 (by gel filtration).  CPA1 subunit is 45 kDa and MW= 36, 000 (by gel filtration).  CPA2 subunit is 124 kDa and MW= 130, 000 (by gel filtration) . The apparent Michaelis constant at pH 7.5 is 1.25 mM for glutamine."],"ac":"EBI-1248649","complexAc":"CPX-579","name":"Carbamoyl-phosphate synthase arginine-specific","species":"Saccharomyces cerevisiae; 559292","ligands":[],"complexAssemblies":["Heterodimer"],"diseases":[],"participants":[{"interactorAC":"EBI-1248663","identifier":"P07258","identifierLink":"https://www.uniprot.org/uniprotkb/P07258/entry","name":"CPA1","description":"Carbamoyl phosphate synthase arginine-specific small chain","stochiometry":"minValue: 1, maxValue: 1","bioRole":"enzyme","bioRoleMI":"MI:0501","bioRoleDefinition":"Molecule catalyzing a modification on its interacting partner.","interactorType":"protein","interactorTypeMI":"MI:0326","interactorTypeDefinition":"A linear polymer of amino acids joined by peptide bonds in a specific sequence.","linkedFeatures":[{"featureAc":"EBI-9256624","participantId":"P03965","name":"cpa1 binding region","featureType":"binding region","featureTypeMI":"MI:0117","featureTypeDefinition":"A region of a molecule or a component of a complex identified as being involved in an interaction. 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