{"systematicName":"4xPFK1:4xPFK2","synonyms":["6-phosphofructokinase","phosphofructokinase","phosphohexokinase","6PF-1-K complex","Pfk-1 complex","PFK complex"],"functions":["Catalyzes the phosphorylation of fructose 6-phosphate to fructose 1,6-bisphosphate in the presence of MgATP, the first irreversible step for glycolysis. The enzyme is stablilized in the presence of Fructose 6-P and exhibits cooperative binding of Fructose 6-P. Its activity is controlled by many allosteric activators and inhibitors (including ATP), showing its crucial role in regulation of glycolytic flux. The enzyme can exist in either the active R-state or inhibited T-state. The effectors either modulate the affinity of Pfk for Fructose 6-P or overcome inhibitory effects. Pfk is an octamer composed of 4 alpha and 4 beta subunits."],"properties":["MW = 835 kDA (approximately). Each subunit is approximately 105 kDa. The native octameric structure has a sedimentation coefficient of 21 S. The molecule is elongated with length of 24 nm and width of 17 nm. There is mild asymmetry between the top and bottom tetramers, each composed of 2 alpha and 2 beta subunits."],"ac":"EBI-1212614","complexAc":"CPX-554","name":"6-phosphofructokinase complex","species":"Saccharomyces cerevisiae; 559292","ligands":[],"complexAssemblies":["Heterooctamer"],"diseases":[],"participants":[{"interactorAC":"EBI-1212625","identifier":"P16861","identifierLink":"https://www.uniprot.org/uniprotkb/P16861/entry","name":"PFK1","description":"ATP-dependent 6-phosphofructokinase subunit alpha","stochiometry":"minValue: 4, maxValue: 4","bioRole":"enzyme","bioRoleMI":"MI:0501","bioRoleDefinition":"Molecule catalyzing a modification on its interacting partner.","interactorType":"protein","interactorTypeMI":"MI:0326","interactorTypeDefinition":"A linear polymer of amino acids joined by peptide bonds in a specific sequence.","linkedFeatures":[{"featureAc":"EBI-9818865","participantId":"P16862","name":"pfk1 binding region","featureType":"binding region","featureTypeMI":"MI:0117","featureTypeDefinition":"A region of a molecule or a component of a complex identified as being involved in an interaction. 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