The use of medicinal plants like T. tetraptera in the treatment of various human ailments such as respiratory infections, convulsions, and rheumatism has gained medical concern for the past few years especially in Africa [23,24], owing to the increasing cost and resistance of pharmaceutical antibiotics [19]. Parts of the plant, thus the leaf, the stem bark, and the fruits play crucial roles in antimicrobial activity due to the presence of phytochemicals [19,23,25]. Leaf extract of T. tetraptera has been known to have antimicrobial activity against Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, and Candida albicans [25]. On the other hand, the stem bark extract of T. tetraptera has also been reported to have higher antimicrobial activity against S. aureus, Streptococcus pneumonia, and Candida spp. as compared to the leaf [23]. This means the intermittent use of the parts of the plant could be employed in the treatment of several bacterial and fungal infections due to its antimicrobial activities [23]. Several studies have reported the antibacterial activity of T. tetraptera extracts against different bacteria. This effect may vary depending on the type of solvent employed in the extraction of the active compounds of the plant and the type of bacteria subjected to the treatment. Studies from Enabulele and Ugha [26] revealed that aqueous extracts of the fruit and seed of T. tetraptera can inhibit Klebsiella pneumoniae and S. aureus compared to ethanolic extract. Similar to antibacterial activity, extracts of T. tetraptera exert antifungal action on various pathogenic fungi. This effect may also be dependent on the parts of the plant as well as the solvent used for extraction. Anyamele et al. [22] revealed that aqueous fruit extract of T. tetraptera can inhibit Aspergillus and Mucor but is ineffective against Rhizopus, yet petroleum ether and ethanol extracts could inhibit the growth of Rhizopus [19]. This biological property of the plant makes it unique, and its usage should be encouraged in ethnomedicine.

Antioxidant activity is one of the most active biological properties of T. tetraptera which contributes to its usage in ethnomedicine since it acts as a reducing agent, preventing cell damage by neutralizing free radicals and inhibiting further oxidation [5]. This function is interplayed by the stem bark, fruit, and leaf of the plant. Famobuwa et al. [27] established that the stem bark of the plant has a lot of antioxidant activities. In addition, Koma et al. [23] also reported that the stem bark of the plant exhibits stronger free radical scavenging activity than the leaf. This property of T. tetraptera agrees with the assertion that the efficacy of the fruit and the stem bark of T. tetraptera in its bioactivities may be attributed to its potential antioxidant activity [27]. That notwithstanding, Adusei et al. [5] revealed that the pulp of T. tetraptera has more antioxidant activity when compared to the whole fruit and the seeds. The presence of phenolic compounds in plant materials is one of the chief contributors to the antioxidant properties shown by plant products. By employing HPLC/LC-MS analysis on ultrasound-assisted T. tetraptera dry fruit extract, Dzah [28] determined twenty-four (24) phenolic compounds. Highest amongst these compounds include orientin Epigallocatechin (310.22 μg/g), (366.00 μg/g), caffeic acid (344.91 μg/g), ferulic acid 4-O-glucuronide (493.02 μg/g), ferulic acid 4-O-glucoside (370.58 μg/g). The extract exhibited a high cellular antioxidant capacity in a dose-dependent manner when compared to gallic acid in HepG2 cells. The minimum inhibition concentrations of T. tetraptera extract and gallic acid were 171.79 and 242.70 μg/mL respectively [28]. Given that the extract of T. tetraptera has a cocktail of phenolic compounds compared to the pure gallic acid, such an effect was expected. Fig. 4 displays how flavonoids can exhibit antioxidant activity.

One other important biological property of T. tetraptera is its anti-inflammatory activities since its use has been very vital in the management of arthritis, inflammation, and rheumatic pains [31]. Different solvent fraction of the leaf carries varying anti-inflammatory activity [31]. In addition, the anti-arthritics property of T. tetraptera fruit is owed to the anti-inflammatory activity of the fruit [24]. The composition of carvacrol in T. tetraptera may be a crucial factor for the anti-inflammatory features displayed by the plant extract [22]. Carvacrol is a monoterpene that is available in the essential oil of different plants [32]. Because of the therapeutic effect of carvacrol, it is used in traditional medicine. Carvacrol has a positive effect in a dose-dependent manner in the reduction of IL-1β and IL-8 when assessed against the aforementioned pro-inflammatory cytokines [32]. Studies have also shown that carvacrol inhibits the expression and inflammatory cytokine levels of cyclooxygenase-2 and nitric oxide synthase [33]. Other compounds available in T. tetraptera such as menthol, methyl eugenol, α-copaene, and octadecanoic acid are known to possess anti-inflammatory activities [22]. Ojewole and Adewunmi [34] examined the anti-inflammatory effects of T. tetraptera fruit extract on egg albumin-induced inflammation of hind paw edema in rats. The results indicated a 50–800 mg/kg dose-dependent reduction of the inflammation induced [34]. These findings substantiate T. tetraptera as a potent medicinal plant that could be exploited in ethno-health care services. Fig. 5 demonstrates the mode of anti-inflammatory action by flavonoids.

Diabetes mellitus (DM) is a metabolic disease that occurs as a result of insulin deficiency [35]. According to the World Health Organization (WHO), about 180 million people worldwide have diabetes [35]. Treatment of diabetes has gained a lot of public attention for the past few years due to the increasing number of diseases. The WHO projects that over 180 million people worldwide will be diabetic by 2030 [35]. Allopathic drugs have been mainly used for the management of DM but recent studies have established the role of T. tetraptera in the management of hyperglycemic cases [35]. The generation of free radicals and reduction of antioxidant capacity have been linked to diabetes mellitus in several studies. In diabetic patients, the decrease in antioxidant parameters is determined [36]. Thus, studies have suggested the application of phytochemicals in the management of diabetes since they can exert free radical scavenging actions and antioxidant potential [36]. Phytochemicals such as flavonoids are found to fight the complexities of diabetes [36] and given that T. tetraptera has a significant concentration of flavonoids, it explains the potential in the management of diabetes [28]. The stem and leaf extract of T. tetraptera is identified to contain limonene, a cyclic monoterpene [22]. The compound limonene possesses both antihyperglycemic and antioxidant effects and in turn, can meliorate the effects of diabetic intricacies [36]. Furthermore, a study by Adesina et al. [3] showed that the fruit extract of T. tetraptera has a better glucose-lowering activity relative to the diabetes mellitus standard drug, glibenclamide. In addition, the root bark extract of T. tetraptera has been recommended as a remedy for diabetes mellitus management [35].

One of the medicinal plants that have evolved as possible alternatives to chemical-based insecticides (with harmful effects on the environment and non-target organisms) is T. tetraptera [37]. T. tetraptera extract has been considered very useful in the control of malaria infection due to its strong larvicidal activity against the larvae of Anopheles gambiae [37]. In addition, the fruit extract of T. tetraptera has been reported to exhibit schizonticidal activity in early infection of Plasmodium falciparum as well as having a strong anti-plasmodia activity [3]. Moreover, African trypanosomiasis and helminthiasis are neglected tropical diseases (NTDS) that mostly affect people in remote areas of Sub-Saharan Africa [38]. Control and elimination of these diseases have been a challenge but Obeng et al. [38] revealed that T. tetraptera fruits and stem bark have anti-trypanosomal and anti-helminthic activities which encourage its usage in the control and management of parasitic infections. Also, T. tetraptera extracts have been reported to have the highest molluscicidal activity amongst some indigenous plants against freshwater snails which helps to control Schistosomiasis or Bilharzia [39]. Moreover, Adesina et al. [3] reported that most parts of the plant including the stem bark, the fruit, and the root carry molluscicidal activity. This makes the use of T. tetraptera in controlling schistosomiasis very promising and needs further investigation.

Chemotherapeutic substances that have been involved in the management and treatment of cancer have been developed from medicinal plants [10] since recent cancer therapeutics have unbearable side effects like hair loss, toxicity, and chemo-resistance [10,40]. The effects of cancer drugs have called for less toxic and more effective ways of managing cancer [40]. As a medicinal plant, T. tetraptera has been reported to be cytotoxic to carcinoma cells and reduce tumor burden [10]. In addition, the methanolic extract of the fruits of the plant has been reported to have an anti-proliferative ability against leukemia and human breast cancer [40]. Dzah [28] studied the cytotoxicity of various concentrations of T. tetraptera extract on the human liver cancer cell line HepG2. In all the concentrations of the extract used (50 μg/mL – 350 μg/mL), T. tetraptera extract had a high cytotoxic effect relative to the same concentrations of gallic acid [28]. The concentration of the extract was proportional to the anti-cancer effect, with a minimum inhibition concentration of 168.06 μg/mL. Polyphenols have been associated with the induction of cellular apoptosis through various mechanisms. Polyphenols may become prooxidants under oxidative stress and produce significant concentrations of free radicals which can induce cancer cell apoptosis [28,41]. In different cancer cells, aqueous extract of T. tetraptera displayed prooxidant action to induce changes in mitochondrial membrane potential [42]. Plant polyphenols can also induce conditions to mimic caloric restriction mimetics in cells, thus inducing molecular mechanisms governing mitochondrial biogenesis and mitophagy [43]. Such effects are some reasons behind the antiproliferative and antitumor characteristics of polyphenols and their potential in cancer management and treatment. This property of T. tetraptera fruit extract is very encouraging and calls for further isolation and categorizing of potential anti-cancer agents from the plant to enhance its use in ethnomedicine [40].

Tetrapleura tetraptera contains significant concentrations of piperazine, 2-hydroxy-gamma-butyrolacetone, 6-octadecenoic acid, octadecanoic acid, and thymol [[44], [45], [46]]. Such compounds possess antiviral properties against certain viruses. Studies have shown that piperazine can inhibit the growth of the highly pathogenic chikungunya virus. The treatment of chikungunya virus with 6 mM piperazine efficiently inhibits the growth of the virus after 72 h [45]. Thymol (2-isopropyl-5-methylphenol) has been long applied as an antiviral agent in traditional medicine. Thymol like camphene and menthol shows significant binding affinity to the spike glycoprotein of SARS-CoV-2, even though it lacks hydrogen binding interaction with the protein. In the preparation of disinfectant against COVID-19, thymol was part of the active ingredients listed by the United States Environmental Protection Agency and the Canadian Government [47]. Thus, the presence of such compounds in T. tetraptera presents a potential use of the plant for combating some viruses. Also, studies have shown that compounds such as octadecanoic acid can produce essential synergistic antiviral effects when combined with a commercial drug like ribavirin with a low cytotoxic effect [48]. With T. tetraptera containing some concentration of octadecanoic acid [22], its combination with other compounds could produce effective antiviral action prior to significant studies.

Tetrapleura tetraptera contains saponins, a naturally occurring compound widely found in the cells of plants. Saponins improve the immune system by decreasing blood lipids and blood glucose response [54]. High levels of saponins diets can inhibit platelet aggregation [54]. Bile acids are released into the intestine when food is consumed [18]. The presence of saponins in T. tetraptera offers detergent properties that enable their attachment to release bile and halt the reabsorption of bile acids produced by the body [18]. Hence, lowering body cholesterol levels, and reducing the risk of a heart attack.

Saponins possess the ability to activate the mammalian immune system [54]. The significant concentration of saponins in T. tetraptera provides the plant with its immunomodulatory effects. The presence of saponins can help with immunoregulation and the reduction of inflammation and oxidative stress in various disease models [55]. The production of serum antibodies can be promoted by saponins [55]. The chemical composition of T. tetraptera with such an essential component grants the human body capacity to combat viruses and some fungi such as Candida albicans and thrush [18].

Extract of T. tetraptera is employed in the treatment of various ailments in traditional medicine. Its use in malaria treatment is no exception, as it possesses antiplasmodial activity [18]. [56,57] reported the use of T. tetraptera extract in treating malaria in Gabon and Cameroon ([56,57]. Methanolic extract of the bark and roots of T. tetraptera inhibited the growth of Plasmodium falciparum 3D7 at 83.6 and 76.3% respectively [57]. Different groups of phytoconstituents are known to possess antimalarial properties. This includes alkaloids, flavonoids, steroids, and terpenes. Alkaloids in particular are considered essential and potent because of their powerful antimalarial properties. The fruit extract of T. tetraptera contains between 1.88% and 2.22% of alkaloids [18]. This demonstrates the plant's antimalarial actions and utilization in African traditional medicine for malaria therapy.

Tetrapleura tetraptera tumbles amongst 25 different plant species employed in diarrhea treatment [58]. T. tetraptera is used as the basis for several herbal remedies because of the astringent properties of tannins present in the plant. Tannin-rich plant fruits can be used to alleviate intestinal inflammation, reduce diarrhea, and relieve nausea [18]. T. tetraptera possesses some of the major groups of tannins, including gallotannins (e.g., gallic acid) [59], Ellagitannins (e.g., ellagic acid) [60], and proanthocyanidins or condensed tannins [61] (e.g., catechin, epigallocatechin) [28,62]. These types of tannins have significant effects in the treatment of diarrhea. For instance, the supplementation of gallic acid (400 mg/kg) in piglets reduces diarrhea occurrence [63]. Similarly, ellagic acid is a natural polyphenol drug known to have anti-diarrhea effects. Studies by Chen et al. [64] revealed that the administration of 0.3 mL (10 mg/mL) of ellagic acid in mice protected the ileum against castor oil-induced diarrhea through the activation of the peroxisome proliferator-activated receptor (PPAR) signaling pathway. The presence of ellagic acid in T. tetraptera (151.00 μg/g) with other forms of tannins provides the biological activity of T. tetraptera against diarrhea. Furthermore, other studies have shown that the administration of Actitan-F (a natural molecular complex of tannin) can truncate the duration of acute diarrhea in children [65]. Likewise, the use of chestnut tannins, for example, can reduce neonatal calf diarrhea [65]. Compared to subjects not fed with chestnut tannins, calves treated with tannins experienced a reduction in diarrheic episodes by almost 4 days [66]. The effects of the various types of tannins in the control of diarrhea explain why T. tetraptera has traditionally been utilized to treat diarrhea and gastrointestinal inflammation [18].

Extracts of T. tetraptera have proven significant glucose binding capacity in a concentration-dependent manner. In a study by Eyenga et al. [67], any given concentration from the extract of T. tetraptera showed substantial glucose-binding ability. The glucose adsorption capacity of T. tetraptera is attributed to the high fiber content of the fruit (>40%) (Eyenga et al. [67]. The binding of fibers to free glucose has been one of the mechanisms employed in inducing glucose reduction [67]. Traditionally, the fruit of T. tetraptera is utilized as a culinary spice to cure diabetes and hypertension by locals in Ghana, the Yoruba tribe of Nigeria, and indigenes of the southern and western parts of Cameroon [9]. Many physiological characteristics of phytochemicals are advantageous to the health of humans. Through enhancing lipid metabolism, antioxidant status, capillary function, reducing blood pressure and cholesterol, and boosting glucose metabolism, phytochemicals in plant-based meals can benefit diabetes patients' overall health [50]. Also, the zinc content of T. tetraptera demonstrates its importance in the control of diabetes [68]. Zinc has several benefits for both type-1 and type-2 diabetes [69]. Its usage as a supplement in diabetic patients enhances glycemic control and improves lipid parameters [69].

Through various modes of action such as inhibition of proliferation, invasion, metastasis, inflammation, and activation of apoptosis, cancer can be prevented by flavonoids such as epigallocatechin-3-gallate [70]. Epigallocatechin-3-gallate (EGCG) can induce apoptosis of hypophosphorylated retinoblastoma and avoid invasion and metastasis in pharyngeal carcinoma cells. EGCG can decrease the expression of vascular endothelial growth factor (VEGF) expression and prevent gastric tumor cell proliferation [70]. The availability of EGCG in T. tetraptera endows it with potential antiproliferative activity. Similarly, bioflavonoid like rutin is known to counteract several cancers through mechanisms like oxidative stress, angiogenesis modulation, malignant cell growth inhibition, and cell cycle arrest [71]. T. tetraptera has about 295.97 μg/g of rutin available in the fruit [28] presenting the plant with significant anticancer activity. Also, scopoletin (a coumarin available in various edible plants) presents anticancer activity via multiple mechanisms like modulating cell cycle arrest, inducing apoptosis, and regulating multiple signaling pathways [72]. T. tetraptera possesses scopoletin, which is significant in conferring anticancer properties [72,73]. People susceptible to certain types of cancer may benefit from scopoletin obtained from the application of T. tetraptera extracts. A study by Yuan et al. [74] revealed the inhibitory activity of scopoletin against small-cell lung cancer. Similarly, Shi et al. [75] showed that a derivative of scopoletin exhibits an anti-tumor effect against breast cancer MDA-MB-231 cell lines. The basic chemical structures of some key phytochemicals in T. tetraptera with health properties are shown in Fig. 6.

In the brewing process of sorghum beer (locally known as “Pito”), an alcoholic beverage commonly available in the northern part of Ghana and other West African nations, T. tetraptera is added to the boiled wort. It helps in the extraction of available bioactive compounds and aroma, thus, enhancing the nutritional content and flavor of the brewed “Pito” [7]. Studies have suggested that the extracts from T. tetraptera could ensure smooth and rapid fermentation by increasing yeast nutrition in the wort. To improve yeast nutrition, calcium chloride, gypsum, and other minerals are used in brewing processes to enhance yeast nutrition. Such an approach is not practiced by local brewers of “Pito”, it thus suggested that based on the chemical composition of T. tetraptera its addition indirectly provides the needed minerals for the yeast to ensure ideal fermentation [7,76].

Besides its application in local alcoholic beverage fermentation, T. tetraptera in addition to honey is used to produce a non-alcoholic beverage known as “Natu Prekese drink” [7]. It is claimed that this beverage aids in preventing menstrual pain, boosts the immune system, and improves blood circulation. However, its application as a dietary supplement has not been evaluated by the FDA, and as such the product is not intended to diagnose, treat, cure, or prevent disease or health condition. Similarly [77], produced a non-alcoholic “prekese” beverage extracted syrup from T. tetraptera. The production of the non-alcoholic drink involved milling and boiling the fruits of T. tetraptera. The obtained filtrate was concentrated into a syrup that could be reconstituted by mixing with water and sugar into a ready-to-drink product. Given that the efficiency of most functional fruit drinks in the market is antioxidant level dependent, the produced drink's antioxidant level was determined. Compared to drinks rich in polyphenols available in the USA such as pomegranate juice, red wine, blueberry juice, black cherry juice, and orange juice, the “prekese” drink possessed higher antioxidant activity [77]. Individuals cognizant of the taste, aroma, and color of beverages produced from T. tetraptera have shown a significant penchant for such drinks. The quality of produced “prekese” drinks show a range of properties acceptable by the Ghana Standards Authority for beverages [77].

In addition to the possibility of producing beverages from T. tetraptera, it has the potential to preserve other sources of beverages upon its incorporation as a preservative. The addition of extracts from T. tetraptera (100 mg/mL) to a freshly prepared watermelon juice saw a reduction in fungal and bacterial load compared to unpreserved juice for 7 days [78]. There is increasing market demand for nutritious food free of chemical preservatives and microbially safe. Freshly prepared juices are usually consumed in non-chemically and non-thermally treated forms, thus maintaining most of the nutrients needed by the consumer. Notwithstanding, there is the challenge of controlling potential spores-forming microbes such as Bacillus sp. In such foods.

T. tetraptera extract has a minimum inhibition concentration of 50 mg/mL against Bacillus cereus, a common fruit juice spoilage microbe. This effect thus suggests that the incorporation of T. tetraptera extracts can potentially enhance the shelf-life of freshly prepared fruit juice while maintaining its nutritive value [78]. The growth of microorganisms such as Staphylococcus aureus, Bacillus cereus, and Saccharomyces cerevisiae can also be reduced upon the addition of essential oil from T. tetraptera. The minimum inhibition concentration against these microbes as determined by Ref. [79] were 42, 46, and 36 mg/mL respectively. Essential oil from T. tetraptera can be applied as a natural fruit juice preservative owing to its bactericidal capacity.

Hot water and organic solvent extracts from T. tetraptera can increase the sugar content of fruit juice. The addition of T. tetraptera extracts to pineapple and pawpaw juices saw an increase in the sugar content from 19.5% to 20.0% and 15.0%–16.0% respectively using hot water extraction. Similarly, T. tetraptera extracts using ethanol enhanced the sugar content of pineapple and pawpaw juice by 2% and 1.5% respectively [80]. The increase in sugar content of the fruit juices by the addition of T. tetraptera stems from the high content of glucose (3.25 mg/mL), sucrose (2.62 mg/mL), and fructose (1.82 mg/mL) present in the extracts of T. tetraptera [80]. Given the flavor, antimicrobial potential, and sugar content of T. tetraptera, it stands as a promising candidate in the application of beverages as natural additives instead of synthetic flavoring agents.

T. tetraptera is usually used in traditional dishes mainly because of its flavor. Together with vegetables such as garlic, pepper, and palm fruits, it is used to prepare a Ghanaian local delicacy known as “palm nut” soup [81]. In the southeast region of Nigeria, the pod of T. tetraptera is employed as a spice for seasoning [81]. Owing to the significant antimicrobial properties of T. tetraptera, it can be applied in the preservation of foods such as pork and chicken. By applying root extracts of T. tetraptera (1%v/v) on pork loaded with 1 × 10⁴ CFU/mL of Escherichia coli and Staphylococcus aureus for 6 days, about 99.99% of the microbial load was reduced [82]. The minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) of 12.5 and 25.0 μg/mL were observed against E. coli and 3.125 and 6.25 μg/mL against S. aureus respectively [82]. It such effect, T. tetraptera has a prospect in its usage as a natural preservative in the meat industry.

Frying is one of the major cooking methods widely used around the globe. Coconut oil is a common example of oil mostly employed in frying. A challenge associated with food prepared with oil is rancidity. Rancidity is related to the odor and off-flavor of oil over a period [83]. This is usually caused by lipid oxidation (reaction of oil and fat with molecular oxygen). Thus, an integral property applied in considering the use of oil in foods is their oxidative stability. To ensure oxidative stability, synthetic antioxidants such as butylated hydroxytoluene (BHT) are used in enhancing the oxidative stability of cooking oils. The use of T. tetraptera in coconut and palm kernel oils can serve as a natural antioxidant in the prevention of oxidative rancidity [83]. After treating both coconut and palm kernel oil with 0.01% of T. tetraptera for 12 days [83], observed a reduction in the p-anisidine value to 4.47 ± 0.65 from 6.45 ± 2.83 and 3.12 ± 0.47 from 5.27 ± 0.55 respectively. p-anisidine values like oxidative stability and peroxide value are indicators of oil quality. It suggests oil deterioration during frying and relates directly with the presence of aldehydes and ketones presence (organics compounds responsible for the rancid taste and flavor of fatty substances). Thus, a lower p-anisidine value of coconut and palm kernel oil after treatment with T. tetraptera shows its capacity to be employed in oil preservation.

T. tetraptera has the capacity to augment the shelf-life of perishable vegetables such as tomatoes and pepper after treatment with aqueous extracts of T. tetraptera. The addition of different concentrations of the extract (0.25 mg/mL - 1 mg/mL) correlated with the increased shelf-life of pepper and tomatoes with increasing concentration [84]. Treatment of tomatoes and pepper with an extract concentration of 1 mg/mL resulted in almost double the shelf life of untreated vegetables. Such effects of aqueous extract from T. tetraptera are attributed to the presence of antimicrobial, phytochemical, and antioxidant properties [84].

Traditionally, T. tetraptera is used in the treatment of cancer, diabetes, and other diseases. As such studies have explored the application of extracts of T. tetraptera against various cancer cell lines to validate the cytotoxic effects. Dichloromethane methanol extract of T. tetraptera fruits, exhibited cytotoxic effects against B16–F1 murine melanoma cells, SKMel-505 BRAF wildtype melanoma cells, MaMel-80a BRAF-V600E homozygous mutant melanoma cells, and SKMel-28 BRAF-V600E homozygous mutant melanoma cells [85]. The lethal dose (LD₅₀) determined from this extract was above 5000 mg/kg body weight. Furthermore, the methanolic extract of T. tetraptera has been employed as an antiproliferative agent against cancer cell lines [40]. evaluated the antiproliferative properties of T. tetraptera against MCF-7, Chang liver cell, and Jurgkat cell lines and observed a positive cytotoxic effect on these cells at a concentration above 100 μg/mL using whole fruit extract. Various fractions of the extract may exhibit different degrees of antiproliferative activity on the applied cell lines [40]. The aforementioned studies show the potential of T. tetraptera extracts as an anticancer agent.

Hyperuricemia is a condition related to an elevated level of uric acid in the blood. The increase in the concentration of uric acid in the body is associated with higher activity of xanthine oxide [86]. Application of T. tetraptera fruit extracts showed inhibitory effects against Fe²⁺ and xanthine oxidase (XO) induced lipid peroxidation. Treatment of induced lipid peroxidation in the lungs, liver, and kidney of rats with different concentrations of T. tetraptera extracts saw a reduction in XO activity with increasing concentrations of T. tetraptera fruit extracts. By using 60 μg/mL of T. tetraptera extract, about 80%, 60%, and 55% of xanthine oxidase inhibition was observed by Ref. [86] in the lungs, kidney, and liver respectively of induced lipid oxidation rats. Higher lipid peroxidation inhibition was reported in rats induced with Fe²⁺. Inhibition of about 82%, 81%, and 60% was observed for the kidney, lungs, and liver respectively. A similar dose-dependent inhibition effect on lipid oxidation of hepatic tissue can occur upon treatment with ethanolic extract of T. tetraptera fruit peel, as reported by Ref. [44]. The presence of phytochemicals, DPPH radical scavenging ability, and nitric oxide radical scavenging ability of the extracts of T. tetraptera is suggested to impact the inhibition of lipid peroxidation. Compared to gallic acid and ascorbic acid, T. tetraptera exhibits low scavenging capacity and in turn low lipid oxidation inhibition [44]. The lipid peroxidation inhibitory capacity of T. tetraptera fruit extract indicates the therapeutic application of T. tetraptera against hyperuricemia and related conditions in a dose-dependent manner [86].

Plants in general have extensive applications in the traditional African setting for the treatment or management of cognitive dysfunction. The presence of significant amounts of p-coumaric acid, rutin, catechin, quercetin, ellagic acid, gallic acid, and chlorogenic acid of T. tetraptera extracts has been linked to the enhanced cognition of host organisms [87]. Studies from Ref. [87] suggest that the administration of T. tetraptera extracts can improve memory indices and prevent scopolamine-induced memory deficits in rats. Scopolamine is a psychoactive drug applied to induce amnesia experimentally. Its application can reduce spatial and non-spatial memory in rats, however, pretreatment of rats with 300 mg/kg aqueous T. tetraptera extracts can reverse declined memory indices caused by scopolamine. This shows the prophylactic ability of T. tetraptera against cognitive impairment in amnesia-induced rats.

By decreasing the elevated activity of Alanine Transaminase (ALT), Aspartate Transaminase (AST), and phenylalanine ammonia-lyase (PAL) in serum, T. tetraptera can be used for the prevention of liver damage. Subjecting rats to CCl₄ treatment results in reduced glutathione, superoxide dismutase (SOD), and catalase (CAT) levels. Oral administration of T. tetraptera extract in liver injury-induced rats saw an increase in the glutathione, SOD, and CAT activity whereas decreasing the malondialdehyde (MDA) levels compared to the untreated subjects. A microphotograph of the rats’ liver showed a reversal to the normal state of the injured rats after T. tetraptera treatment of 100 mg/kg for 48 h [88]. The significant concentrations of phytochemicals available in T. tetraptera extracts and their corresponding oxidation scavenging ability give it treatment and preventive capacity against several ailments.

Other than the extensive applications of T. tetraptera in food and medicine, it can also be used to supplement feeds for farm animals and pets for various reasons [89]. Owing to the challenges of the use of antibiotics in feeds for animals, T. tetraptera can be employed as a feed supplement in place of antibiotics. By fortifying broiler chicken feed with 0.2% T. tetraptera fruit powder [89], observed an increase in body weight relative to chickens fed with a diet containing 0.4% T. tetraptera and a diet with no supplementation. The said study observed a similar feed conversion ratio (FCR) for chickens fed with an antibiotic-supplemented diet (FCR = 2.02) and 0.2 % T. tetraptera-supplemented diet (FCR = 2.03). With a lower cost of production and improved growth performance, T. tetraptera powder could be employed as an antibiotic growth promoter in producing chicken meat free of antibiotic residues and preventing the creation of antibiotic resistance.

Similarly, the employment of T. tetraptera as a feed additive in rabbit bucks' diet resulted in a reduction in the feed conversion ratio relative to percent dosage. The Lowest feed conversion ratio (2.43 ± 0.03) was recorded when the subjects were fed with 1% of T. tetraptera compared to FCR (3.76 ± 0.50) of subjects without the addition of T. tetraptera [90]. High livestock production efficiency is associated with lower FCR [90]. Thus, the reduction in FCR in the rabbits after the inclusion of 1% of T. tetraptera reveals the potential of T. tetraptera as a natural feed additive for livestock. T. tetraptera can also serve as a feed additive for a diet of West African Dwarf rams. Supplementation of Panicum maximum with varying concentrations of T. tetraptera increased glutathione peroxidase and superoxidase after the feeding trial. The diet formulation containing up to 2% of T. tetraptera improved the thermo-physiological responses (pulse rate and breathing rate) in the rams and decreased the level of oxidative stress [91].