Enzyme

6.3.4.2 - CTP synthase (glutamine hydrolyzing)

Alternative Name(s)
  • UTP--ammonia ligase.
  • CTP synthetase.

Catalytic Activity

ATP + H2O + L-glutamine + UTP = ADP + CTP + 2 H(+) + L-glutamate + phosphate

Cofactors

There are no Cofactors for this Enzyme

Reaction Mechanism

There are no Reaction Mechanism for this Enzyme

Reaction Parameters

  • Kinetic Parameters
    Organism KM Value [mM] Substrate Comment
    Saccharomyces cerevisiae 0.04 UTP pH 8.0, 30°C, phosphorylated enzyme, in presence of 2 mM ATP
    Trypanosoma brucei 0.07 ATP
    Rattus norvegicus 0.07 GTP
    Bos taurus 0.07 GTP
    Chlamydia trachomatis 0.097 ATP pH 7.2
  • Temperature
    Organism Temperature Range Comment
    Anoxybacillus gonensis 70 63% of maximum activity
  • pH
    Organism pH Range Comment
    Escherichia coli 7 - 10 ammonia-dependent activity rises linearly from pH 7 to pH 10
    Mus musculus 7.3 - 9.5 pH 7.3: about 35% of maximal activity, pH 9.5: about 40% of maximal activity
    Trypanosoma brucei 7.3 assay at
    Saccharomyces cerevisiae 7.5 - 9 pH 7.5: about 70% of maximal activity, pH 9.0: about 55% of maximal activity, dUTP-dependent activity

Associated Proteins

Protein name Organism
CTP synthase 2 Human
CTP synthase Fruit fly
CTP synthase 1 Human
CTP synthase 1-A African clawed frog
Putative CTP synthase Ureaplasma parvum serovar 3 (strain ATCC 700970)

Citations