Enzyme - D-alanine--D-alanine ligase

Alternative Name(s)
  • Alanine:alanine ligase (ADP-forming).
  • Alanylalanine synthetase.
  • D-alanylalanine synthetase.
  • D-Ala-D-Ala synthetase.
  • D-alanyl-D-alanine synthetase.

Catalytic Activity

ATP + 2 D-alanine = ADP + D-alanyl-D-alanine + H(+) + phosphate


There are no Cofactors for this Enzyme

Reaction Mechanism

    D-alanine--D-alanine ligase (EC: is a bacterial enzyme involved in cell-wall biosynthesis. It participates in forming UDP-N-acetylmuramoyl pentapeptide, the peptidoglycan precursor.

    This enzyme catalyses the transfer of a phosphate group from ATP to carboxyl oxygen of D-alanine, forming an intermediate, D-alanyl acylphosphate. Then the acyl group is transferred from D-alanyl acylphosphate to the amine group of the second D-alanine substrate, releasing inorganic phosphate.
    Catalytic Residues
    AA Uniprot Uniprot Resid PDB PDB Resid
    Asn P07862 272 2dln 272
    Ser P07862 150 2dln 150
    Arg P07862 255 2dln 255
    Asp P07862 257 2dln 257
    Glu P07862 270 2dln 270
    Glu P07862 15 2dln 15
    Gly P07862 276 2dln 276
    Tyr P07862 216 2dln 216

Reaction Parameters

There are no kinetic parameters information for this Enzyme

Associated Proteins

Protein name Organism
D-alanine--D-alanine ligase Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
D-alanine--D-alanine ligase B Escherichia coli (strain K12)
D-alanine--D-alanine ligase A Escherichia coli (strain K12)
Bifunctional enzyme MurC/Ddl Chlamydia trachomatis (strain D/UW-3/Cx)
D-alanine--D-alanine ligase (D-alanylalanine synthetase) (D-Ala-D-Ala ligase) Ralstonia solanacearum CFBP2957