Enzyme - Glutamate--cysteine ligase

Alternative Name(s)
  • Gamma-glutamylcysteine synthetase.
  • Gamma-glutamyl-L-cysteine synthetase.

Catalytic Activity

ATP + L-cysteine + L-glutamate = ADP + H(+) + L-gamma-glutamyl-L-cysteine + phosphate


There are no Cofactors for this Enzyme

Reaction Mechanism

There are no Reaction Mechanism for this Enzyme

Reaction Parameters

  • Kinetic Parameters
    Organism KM Value [mM] Substrate Comment
    Synechocystis sp. 0.0086 ATP pH 7.5, 25°C, mutant R248K
    Pseudoalteromonas haloplanktis 0.044 ATP pH 8.0, 15°C, recombinant enzyme
    Escherichia coli 46.6 ATP pH 8.0, 25°C, mutant C106S/C164S/C205S/C223S/C357S/C372S/C395S/C433S/C439S/S372C/S395Y
  • Temperature

    There are no reaction parameters information for this Enzyme.

  • pH
    Organism pH Range Comment
    Nicotiana tabacum 7 - 9 50% of maximal activity at pH 7.0 and pH 9.0
    Pseudoalteromonas haloplanktis 7 - 9 narrow pH range, highest activity at pH 7.8-8.6

Associated Proteins

Protein name Organism
Glutamate--cysteine ligase EgtA Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)
Glutamate--cysteine ligase, chloroplastic Mouse-ear cress
Glutamate--cysteine ligase A, chloroplastic Rice
Putative glutamate--cysteine ligase 2-1 Arthrobacter sp. (strain FB24)
Glutamate--cysteine ligase B, chloroplastic Rice