Cofactors

There are no Cofactors for this Enzyme

Reaction Mechanism

enoyl-CoA hydratase By Reference

Enoyl-CoA hydratase catalyses the hydration of trans-2-enoyl-CoA thiolesters to the corresponding 3(S)-hydroxyacyl-CoA compounds, the second step in the fatty acid beta-oxidation pathway. The enzymes degrade fatty acids of variable tail length from C4 up to at least C16, but the rate of the reaction decreases with increasing tail length. Enoyl-CoA hydratase is a member of the corotonase superfamily of enzymes.

Despite the thermodynamic preference for the aromatic conjugated unsaturated substrate, the enzyme has been shown to catalyse the dehydration of 3-hydroxy-3-phenylpropanoyl-CoA to cinnamoyl-CoA.




• Summary
• Step 1
• Step 2
• Products

Glu164 deprotonates the catalytic water, which adds to the C3 of the substrate. The intermediate collapses and the C2 of the substrate deprotonates Glu164.

Catalytic Residues

AA	Uniprot	Uniprot Resid	PDB	PDB Resid
Gly	P14604	141	1ey3	109
Ala	P14604	98	1ey3	66
Glu	P14604	144	1ey3	112
Glu	P14604	164	1ey3	132

Step Components

intermediate formation, intermediate terminated, bimolecular nucleophilic addition, proton transfer, native state of enzyme regenerated, overall reactant used, overall product formed



Step 1.

Glu164 deprotonates the catalytic water, which adds to the C3 of the substrate.



Step 2.

The intermediate collapses and the C2 of the substrate deprotonates Glu164.



Products.

The products of the reaction.

View Reaction Mechanisms in M-CSA

Reaction Parameters

• Kinetic Parameters

  Organism	KM Value [mM]	Substrate	Comment
  Metallosphaera sedula	260	crotonoyl-CoA	at pH 7.0 and 70°C

• Temperature

  There are no reaction parameters information for this Enzyme.

• pH

  Organism	pH Range	Comment
  Cupriavidus necator	6 - 7

Associated Proteins

Citations

• Biosynthesis of Vanillin by Rational Design of Enoyl-CoA Hydratase/Lyase.
• Boosting Lipid Production in the Diatom Phaeodactylum tricornutum by Knockdown of the Enoyl CoA Hydratase Using CRISPR Interference
• Enoyl-CoA hydratase/3-hydroxyacyl CoA dehydrogenase is essential for the production of DHA in zebrafish.
• Neuropathological Findings in Short-Chain enoyl-CoA Hydratase 1 Deficiency (ECHS1D): Case Report and Differential Diagnosis.
• Clinical improvements after treatment with a low-valine and low-fat diet in a pediatric patient with enoyl-CoA hydratase, short chain 1 (ECHS1) deficiency.
• Nicotinamide Mononucleotide Alleviates Cardiomyopathy Phenotypes Caused by Short-Chain Enoyl-Coa Hydratase 1 Deficiency.
• Loss of mitochondrial fatty acid β-oxidation protein short-chain Enoyl-CoA hydratase disrupts oxidative phosphorylation protein complex stability and function.
• Developing efficient vanillin biosynthesis system by regulating feruloyl-CoA synthetase and enoyl-CoA hydratase enzymes.
• Characterization of an (R)-specific enoyl-CoA hydratase from Streptomyces sp. strain CFMR 7: A metabolic tool for enhancing the production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate).
• Crystal structure of enoyl-CoA hydratase from Thermus thermophilus HB8.
• Exploring triheptanoin as treatment for short chain enoyl CoA hydratase deficiency.