126.96.36.199 - Phosphopyruvate hydratase
- 2-phosphoglycerate dehydratase.
(2R)-2-phosphoglycerate = H2O + phosphoenolpyruvate
Yeast enolase (2-phospho-D-glycerate hydrolase) is a metalloenzyme which catalyses the reversible dehydration of D-2-phos-phoglycerate (PGA) to phosphoenolpyruvate (PEP). The enzyme has an absolute requirement for the presence of a divalent cation, as is characteristic of the enolase family.
Mitochondrial targeting of tRK1 in yeast is achieved by the successive actions of enolase 2 and the precursor of the mitochondrial lysyl-tRNA synthetase (preMSK). At the mitochondrial outer membrane, preMSK takes over enolase to start the import process properly; A fraction of the canonical tRNA L-form tRK1 pool is deviated from the cytosolic translation process by the enolase 2, which favours the tRNA conformational change leading to the formation of the F-form.
|AA||Uniprot||Uniprot Resid||PDB||PDB Resid|
intermediate formation, intermediate terminated, assisted keto-enol tautomerisation, rate-determining step, proton transfer, dehydration, overall reactant used, unimolecular elimination by the conjugate base, intermediate collapse, overall product formed
Organism KM Value [mM] Substrate Comment Klebsiella pneumoniae 23.5 phosphoenolpyruvate in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl Homo sapiens 48.5 phosphoenolpyruvate in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl Saccharolobus solfataricus 58.44 phosphoenolpyruvate 65°C, pH not specified in the publication Theileria annulata 350 2-phospho-D-glycerate 1.5 mM MgCl2 in 50 mM Tris/HCl (pH 7.4), at 25°C Plasmodium falciparum 520 2-phospho-D-glycerate recombinant, dimeric enzyme
Organism Temperature Range Comment Bacillus anthracis 25 - 45 at pH 6.8, recombinant protein Clostridioides difficile 25 - 80 80°C: 27% of maximal activity Pyrococcus furiosus 40 - 90 no activity at 25°C, activity increases from 40°C to 90°C, the highest assay temperature used
Organism pH Range Comment Brucella abortus 5 - 10 activity range, recombinant enzyme Plasmodium falciparum 5 - 5.5 pH-dependent dissociation reveals that protonation of groups at the intersubunit interface is responsible for dissociation Macaca mulatta 6 - 9 pH 6.0: about 35% of maximal activity, pH 9.0: about 30% of maximal activity Trichinella spiralis 6 - 7.5 Leuconostoc mesenteroides 6 - 8.3 catalytic activity above and below pH 6.8 remains constant at 30% of maximal activity
- Characterization and epitope prediction of phosphopyruvate hydratase from Penaeus monodon (black tiger shrimp).
- cDNA cloning and expression analysis of a phosphopyruvate hydratase gene from the chinese shrimp Fenneropenaeus chinensis.
- Enolase: a metabolic checkpoint behind diverse exhaustion stages of CD8+ T cells in chronic HBV and HCV.
- Molecular cloning of kuruma shrimp Marsupenaeus japonicus phosphopyruvate hydratase and its role in infection by white spot syndrome virus and Vibrio alginolyticus
- Electrochemiluminescence resonance energy transfer between a Ru-ZnMOF self-enhanced luminophore and a double quencher ZnONF@PDA to detect NSE.
- Large-scale preparation of yeast strains expressing condensates derived from a glycolytic enzyme via controlled dissolved oxygen levels under hypoxia.
- Steroid-responsive ocular flutter with truncal ataxia and anti-amino-terminal of α-enolase antibody.
- High-Fat Mouse Model to Explore the Relationship between Abnormal Lipid Metabolism and Enolase in Pancreatic Cancer.
- Pyruvate kinase and phosphopyruvate hydratase as novel IgE reactive proteins in prawn.
- Treatment of the CRND8 mouse model for cerebral amyloid angiopathy, exhibited increased levels of neuron specific enolase in brain tissue following long-term treatment with a modified C5a receptor agonist, accompanied by improved cognitive function.
- Soluble Enolase 1 of Candida albicans and Aspergillus fumigatus Stimulates Human and Mouse B Cells and Monocytes.