Enzyme

3.5.1.14 - N-acyl-aliphatic-L-amino acid amidohydrolase

Alternative Name(s)
  • Hippuricase.
  • Aminoacylase I.
  • Dehydropeptidase II.
  • Acylase I.
  • Benzamidase.
  • Histozyme.
  • Aminoacylase 1.
  • N-acyl-L-amino-acid amidohydrolase.

Catalytic Activity

N-acetyl-L-cysteine-S-conjugate + H2O = acetate + an S-substituted L-cysteine

Cofactors

Zn(2+).

Reaction Mechanism

There are no Reaction Mechanism for this Enzyme

Reaction Parameters

  • Kinetic Parameters
    Organism KM Value [mM] Substrate Comment
    Sus scrofa 23 N-acetyl-L-Cys analysis by HPLC, pH 7.6, 37°C
    Homo sapiens 60 N-acetyl-L-Cys analysis by HPLC, pH 7.6, 37°C
    Thermococcus litoralis 19.6 N-benzoyl-L-valine F251K mutant protein, pH 8.0, 55°C
  • Temperature
    Organism Temperature Range Comment
    Sus scrofa 20 - 80
    Deinococcus radiodurans 20 - 90
    Alcaligenes sp. 25 - 75
    Geobacillus sp. 30 - 90 30°C: about 37.6% of maximal activity, 90°C: about 27% of maximal activity
    Pyrococcus horikoshii 60 - 100
  • pH
    Organism pH Range Comment
    Deinococcus radiodurans 3.7 - 10.8 using different buffers containing N-acetyl-D-Homophenylalanine or N-acetyl-L-Homophenylalanine
    Alcaligenes sp. 4 - 6.5
    Geobacillus sp. 4 - 11 pH 4.0: 59% of maximal activity, pH 11.0: 19% of maximal activity
    Sus scrofa 4.5 - 7.2 activity range, substrate N-acetyl-L-methionine
    Pyrococcus horikoshii 6 - 8.5

Associated Proteins

Protein name Organism
Aminoacylase-1 Human
Aminoacylase-1B Rat
N-acyl-L-amino acid amidohydrolase Geobacillus stearothermophilus
Aminoacylase-1A Rat
Acylamidase Rhodococcus erythropolis

Citations