Cofactors

There are no Cofactors for this Enzyme

Reaction Mechanism

isochorismatase By Reference

Isochorismatase, also known as 2,3 dihydro-2,3 dihydroxybenzoate synthase, catalyses the conversion of isochorismate, in the presence of water, to 2,3-dihydroxybenzoate and pyruvate, via the hydrolysis of a vinyl ether, an uncommon reaction in biological systems. Isochorismatase is part of the phenazine biosynthesis pathway. Phenazines are antimicrobial compounds that provide the competitive advantage for certain bacteria.




The carboxylate of Asp38 acts as a general acid to protonate the substrate, yielding a carbocation/oxocarbonium ion that is then rapidly hydrated to form a hemiketal intermediate, which then decomposes spontaneously to products.

Catalytic Residues

AA	Uniprot	Uniprot Resid	PDB	PDB Resid
Asp	P0DPB9	38	1nf9	38
His	P0DPB9	37	1nf9	37
Lys	P0DPB9	122	1nf9	122
View Reaction Mechanisms in M-CSA

Reaction Parameters

• Kinetic Parameters

  Organism	KM Value [mM]	Substrate	Comment
  Escherichia coli	0.015	isochorismate
  Pseudomonas aeruginosa	0.1	isochorismate	mutant R54K, pH 7.5, 30°C

• Temperature

  There are no reaction parameters information for this Enzyme.

• pH

  There are no reaction parameters information for this Enzyme.

Associated Proteins

Citations

• Cloning and expression of the phenazine-1-carboxamide hydrolysis gene pzcH and the identification of the key amino acids necessary for its activity.
• Catabolite repression control protein antagonist, a novel player in Pseudomonas aeruginosa carbon catabolite repression control.
• Structural and Functional Characterization of the Ureidoacrylate Amidohydrolase RutB from Escherichia coli.
• Whole Genome Analysis and Assessment of the Metabolic Potential of Gordonia rubripertincta Strain 112, a Degrader of Aromatic and Aliphatic Compounds.
• Converting the E. coli Isochorismatase Nicotinamidase into γ-Lactamase.
• The versatile defender: exploring the multifaceted role of p62 in intracellular bacterial infection.
• Evidence of an intracellular interaction between the Escherichia coli enzymes EntC and EntB and identification of a potential electrostatic channeling surface.
• Genomics of Acinetobacter baumannii iron uptake.
• ISOC1 is a novel potential tumor suppressor in hepatocellular carcinoma.
• An unconventionally secreted effector from the root knot nematode Meloidogyne incognita, Mi-ISC-1, promotes parasitism by disrupting salicylic acid biosynthesis in host plants.
• Wastewater bacteria remediating the pharmaceutical metformin: Genomes, plasmids and products.