Cofactors

There are no Cofactors for this Enzyme

Reaction Mechanism

beta-galactosidase By Reference

Catalyses the hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactoside. Belongs to the glycosyl hydrolase 2 family.




Glu537 initiates a nucleophilic attack on the substrate, which eliminates the first product. Glu461 activates the catalytic water, which attacks the enzyme-substrate adduct, eliminating the final product and returning the enzyme to its native state.

Catalytic Residues

AA	Uniprot	Uniprot Resid	PDB	PDB Resid
His	P00722	358	4v40	357
Glu	P00722	462	4v40	461
Glu	P00722	538	4v40	537
His	P00722	392	4v40	391
Glu	P00722	417	4v40	416
Asp	P00722	202	4v40	201
His	P00722	419	4v40	418
Phe	P00722	602	4v40	601
Asn	P00722	605	4v40	604
Asn	P00722	598	4v40	597
View Reaction Mechanisms in M-CSA

Reaction Parameters

There are no kinetic parameters information for this Enzyme

Associated Proteins

Citations

• Effect of Chemical Modification on the Distribution of Electrophoretic Mobilities of Individual Molecules of E. coli beta-Galactosidase
• Fidelity-oriented fluorescence imaging probes for beta-galactosidase: From accurate diagnosis to precise treatment.
• Streamlining the Detection of Human Thyroid Receptor Ligand Interactions with XL1-Blue Cell-Free Protein Synthesis and Beta-Galactosidase Fusion Protein Biosensors.
• Quantification of beta-galactosidase activity as a marker of radiation-driven cellular senescence.
• Alpha-amylase and alphaglucosidase inhibitory properties, beta-galactosidase activity, and probiotic potential of lactic acid bacteria and bifidobacteria from Apis mellifera intermissa and its products.
• Acceleration of lactose hydrolysis using beta-galactosidase and deep eutectic solvents.
• Beta-galactosidase gene family genome-wide identification and expression analysis of members related to fruit softening in melon (Cucumis melo L.).
• Whole-mount Senescence-Associated Beta-Galactosidase (SA-β-GAL) Activity Detection Protocol for Adult Zebrafish.
• Developing a far-red fluorogenic beta-galactosidase probe for senescent cell imaging and photoablation.
• Disentangling molecular and clinical stratification patterns in beta-galactosidase deficiency.
• One-step purification of a recombinant beta-galactosidase using magnetic cellulose as a support: Rapid immobilization and high thermal stability.