Cofactors

There are no Cofactors for this Enzyme

Reaction Mechanism

3'(2'),5'-bisphosphate nucleotidase By Reference

Catalyses the removal of the 3'-phosphate from adenosine 5'-phosphosulfate to reform AMP. This reaction helps drive the sulfur incorporation cycle for pathways such as methionine biosynthesis. Sensitivity of this essential enzyme to sodium and other metal ions results is responsible for characterisation of this enzyme as a salt tolerance protein [PMID:10205895]. Some members of this family are active also as inositol 1-monophosphatase.




• Summary
• Step 1
• Step 2
• Step 3
• Products

A water molecule bound to the first site magnesium ion is activated through a proton relay with Asp49 and Thr147. It then attacks the 3'-phosphate group, eliminating the product phosphate and a negatively charged intermediate which subsequently is reprotonated from bulk solvent via a proton relay involving the second ribose hydroxyl group and a water bound to the second magnesium site.

Catalytic Residues

AA	Uniprot	Uniprot Resid	PDB	PDB Resid
Asp	P32179	142	1qgx	142
Ile	P32179	144	1qgx	144
Asp	P32179	145	1qgx	145
Glu	P32179	72	1qgx	72
Asp	P32179	49	1qgx	49
Thr	P32179	147	1qgx	147
Asp	P32179	294	1qgx	294

Step Components

overall product formed, proton transfer, overall reactant used, bimolecular nucleophilic addition, native state of enzyme regenerated, inferred reaction step, proton relay



Step 1.

Asp49 activates the catalytic water through Thr147. The activated species then initiates a nucleophilic attack on the magnesium bound phosphate, eliminating it from the diphosphate substrate.



Step 2.

The intermediate is reprotonated from the bulk solvent via a proton relay through the other ribose sugar, the second magnesium bound water and thence bulk solvent.



Step 3.

Inferred return step.



Products.

The products of the reaction.

View Reaction Mechanisms in M-CSA

Reaction Parameters

There are no kinetic parameters information for this Enzyme

Associated Proteins

Citations

• Creation of salt-insensitive 3'(2'),5'-bisphosphate nucleotidase by modeling and mutagenesis approach.
• FIERY1 regulates light-mediated repression of cell elongation and flowering time via its 3'(2'),5'-bisphosphate nucleotidase activity.
• Complementation of an E. coli cysteine auxotrophic mutant for the structural modification study of 3′(2′),5′-bisphosphate nucleotidase
• Complementation of an E. coli cysteine auxotrophic mutant for the structural modification study of 3'(2'),5'-bisphosphate nucleotidase.
• Molecular cloning and biochemical characterization of a 3'(2'),5'-bisphosphate nucleotidase from Debaryomyces hansenii.
• Sulfur metabolism in subtropical marine mangrove sediments fundamentally differs from other habitats as revealed by SMDB.
• Multiplex CRISPR/Cas9-mediated genome editing to address drought tolerance in wheat.
• The Pro-Oncogenic Sphingolipid-Metabolizing Enzyme β-Galactosylceramidase Modulates the Proteomic Landscape in BRAF(V600E)-Mutated Human Melanoma Cells.
• Combined analyses of transcriptome and metabolome reveal the mechanism of exogenous strigolactone regulating the response of elephant grass to drought stress.
• A mutation in Arabidopsis SAL1 alters its in vitro activity against IP₃ and delays developmental leaf senescence in association with lower ROS levels.
• Proteomic differences in seminal fluid of social insects whose sperm differ in heat tolerance