Cofactors

There are no Cofactors for this Enzyme

Reaction Mechanism

UDP-N-acetylglucosamine pyrophosphorylase By Reference

The bifunctional enzyme GlmU is able to catalyse two steps in the pathway of bacterial cell wall synthesis:

1. The C-terminal domain converts glucosamine-1-phosphate to N-acetyl glucosamine-1-phosphate using Acetyl CoA as the donor
2. The N-terminal domain (this entry) utilises UTP to activate the N-acetyl glucosamine-1-phosphate forming UDP-acetyl-glucosamine and pyrophosphate.

Interest in the processes catalysed by this enzyme stems from its potential as an antibiotic target.




• Summary
• Step 1
• Step 2
• Products

The mechanism of the transfer of UDP to N acetylglucosamine-1-phosphate proceeds via nucleophilic attack from the phosphate oxygen of the phosphate moiety on the alpha phosphate producing a pentavalent phosphate transition state. This is stabilised by Mg2+ at the active site and Arg 18, and collapses to release the beta and gamma phosphates as pyrophosphate.

Catalytic Residues

AA	Uniprot	Uniprot Resid	PDB	PDB Resid
Arg	P0ACC7	18	1hv9	18

Step Components

overall reactant used, overall product formed, proton transfer, bimolecular nucleophilic substitution, inferred reaction step



Step 1.

The phosphate of N-acetyl-alpha-D-glucosamine 1-phosphate attacks the alpha phosphate of UTP. This releases diphosphate and forms the product.



Step 2.

The diphosphate group is protonated. The proton may be sourced from the solvent.



Products.

The products of the reaction.

View Reaction Mechanisms in M-CSA

Reaction Parameters

There are no kinetic parameters information for this Enzyme

Associated Proteins

Citations

• N-glycolylated carbohydrates in nature.
• Genomic reconstruction and features of glycosylation pathways in the apicomplexan Cryptosporidium parasites.
• Chimaeribacter arupi a new member of the Yersineacea family has the characteristics of a human pathogen.
• In Silico Probiogenomic Characterization of Lactobacillus delbrueckii subsp. lactis A4 Strain Isolated from an Armenian Honeybee Gut.
• Application of proteomics to determine the mechanism of ozone on sweet cherries (Prunus avium L.) by time-series analysis.
• Computer-Based Identification of Potential Druggable Targets in Multidrug-Resistant Acinetobacter baumannii: A Combined In Silico, In Vitro and In Vivo Study.
• Transcriptome-based strategies for identifying aluminum tolerance genes in popcorn (Zea mays L. var. everta).
• Silencing uridine diphosphate N-acetylglucosamine pyrophosphorylase gene impairs larval development in Henosepilachna vigintioctopunctata.
• Transcriptomic analysis of albendazole resistance in human diarrheal parasite Giardia duodenalis.
• In vitro and in silico assessment of probiotic and functional properties of Bacillus subtilis DE111^®.
• Human Erysipelothrix rhusiopathiae infection via bath water - case report and genome announcement.