Cofactors

There are no Cofactors for this Enzyme

Reaction Mechanism

There are no Reaction Mechanism for this Enzyme

Reaction Parameters

• Kinetic Parameters

  Organism	KM Value [mM]	Substrate	Comment
  Thermus thermophilus	14	ATP	pH 8.0, 75°C
  Mycobacterium tuberculosis	26	ATP	in 50 mM Tris-HCl pH 8.0, at 25°C
  Mycolicibacterium smegmatis	1315	D-ribose 5-phosphate	pH 7.5, 25°C
  Pyrobaculum calidifontis	5183	D-ribose 5-phosphate	pH 10.5, 55°C, recombinant enzyme

• Temperature

  Organism	Temperature Range	Comment
  Methanocaldococcus jannaschii	72 - 95	70% of maximal activity at 72°C and 95°C

• pH

  Organism	pH Range	Comment
  Mycobacterium tuberculosis	4.5 - 9.5	the enzyme shows activity across a broad range of pH levels (4.5-9.5) and is only found to be inactive at high pH 10.5
  Salmonella enterica subsp. enterica serovar Typhimurium	6 - 9.5	very low activity below and above
  Homo sapiens	6.5 - 9.3	At pH 6.5, about 25% and 50% of maximum activity for isoenzymes PRSI and PRSII, respectively. At pH 9.3, about 40% and 60% of maximum activity for isoenzymes PRSII and PRSI, respectively
  [Bacillus] caldolyticus	6.5 - 9.5	pH 6.5: about 25% of maximal activity, pH 9.5: about 80% of maximal activity
  Bacillus subtilis	7 - 9.5	at pH 7 and pH 9.5, about 35% and 85% of the maximal activity, respectively

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Associated Proteins

Citations

• Improvement of an enzymatic cascade synthesis of nicotinamide mononucleotide via protein engineering and reaction-process reinforcement
• Enhancing the biosynthesis of riboflavin in the recombinant Escherichia coli BL21 strain by metabolic engineering.
• Metabolic reprogramming in colorectal cancer: regulatory networks and therapy.
• Metabolic engineering of Escherichia coli for biosynthesis of β-nicotinamide mononucleotide from nicotinamide.
• Toward novel treatment against filariasis: Insight into genome-wide co-evolutionary analysis of filarial nematodes and Wolbachia.
• Transcriptome profiles of high-lysine adaptation reveal insights into osmotic stress response in Corynebacterium glutamicum.
• The role of Dmnt1 during spermatogenesis of the insect Oncopeltus fasciatus.
• Analysis and functional relevance of the chaperone TRAP-1 interactome in the metabolic regulation and mitochondrial integrity of cancer cells.
• Tryptophan plays an important role in yeast's tolerance to isobutanol.
• Construction of the model for predicting prognosis by key genes regulating EGFR-TKI resistance.
• Staphylococcus epidermidis RP62A's Metabolic Network: Validation and Intervention Strategies.