Enzyme
2.3.3.5 - 2-methylcitrate synthase
Alternative Name(s)
- Methylcitrate synthase.
- MCS.
- 2-methylcitrate oxaloacetate-lyase.
- Methylcitrate synthetase.
Catalytic Activity
H2O + oxaloacetate + propanoyl-CoA = (2S,3S)-2-methylcitrate + CoA + H(+)
Cofactors
There are no Cofactors for this Enzyme
Reaction Mechanism
There are no Reaction Mechanism for this Enzyme
Reaction Parameters
-
Kinetic Parameters
Organism KM Value [mM] Substrate Comment Salmonella enterica subsp. enterica serovar Typhimurium 7.05 acetyl-CoA in 50 mM HEPES pH 8.0, at 30°C Aspergillus fumigatus 2900 acetyl-CoA pH 8.0, temperature not specified in the publication, mutant enzyme G352A -
Temperature
Organism Temperature Range Comment Yarrowia lipolytica 10 - 50 Aspergillus nidulans 20 - 70 Rhodothermus marinus 40 - 90 Fusarium solani 49 - 54 maximum activity Fusarium verticillioides 49 - 54 maximum activity -
pH
Organism pH Range Comment Aspergillus nidulans 5.5 - 10.5 Yarrowia lipolytica 6 - 9.5 Fusarium solani 8 - 9 maximum activity Fusarium verticillioides 8 - 9 maximum activity
Associated Proteins
Citations
- Comparative studies of Aspergillus fumigatus 2-methylcitrate synthase and human citrate synthase.
- Cloning, expression, purification and bioinformatic analysis of 2-methylcitrate synthase from Mycobacterium tuberculosis.
- Enhanced production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with modulated 3-hydroxyvalerate fraction by overexpressing acetolactate synthase in Cupriavidus necator H16.
- Proteomic Profiling of Paracoccidioides brasiliensis in Response to Phenacylideneoxindol Derivative: Unveiling Molecular Targets and Pathways.
- New Methylcitrate Synthase Inhibitor Induces Proteolysis, Lipid Degradation and Pyruvate Excretion in Paracoccidioides brasiliensis.
- Preliminary X-ray crystallographic analysis of 2-methylcitrate synthase from Salmonella typhimurium.
- Crystal structure of Salmonella typhimurium 2-methylcitrate synthase: Insights on domain movement and substrate specificity.
- Systems-Wide Dissection of Organic Acid Assimilation in Pseudomonas aeruginosa Reveals a Novel Path To Underground Metabolism.
- In silico detection and characterization of novel virulence proteins of the emerging poultry pathogen Gallibacterium anatis.
- Neisseria meningitidis Sibling Small Regulatory RNAs Connect Metabolism with Colonization by Controlling Propionate Use.
- Systems-wide dissection of organic acid assimilation in Pseudomonas aeruginosa reveals a novel path to underground metabolism