Enzyme - Methylenetetrahydrofolate dehydrogenase (NADP(+))

Alternative Name(s)

There are no alternative names for this Enzyme

Catalytic Activity

(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADP(+) = 5,10-methenyltetrahydrofolate + NADPH


There are no Cofactors for this Enzyme

Reaction Mechanism

    Enzymes involved in tetrahydrofolate metabolism are of particular pharmaceutical interest, as their function is crucial for amino acid and DNA biosynthesis.

    In eukaryotes, the enzyme that performs this reaction occurs as a trifunctional enzyme that also has methylenetetrahydrofolate dehydrogenase (NADP+) (EC and formate-tetrahydrofolate ligase (EC activity. In some prokaryotes, it occurs as a bifunctional enzyme that also has dehydrogenase (EC activity or formiminotetrahydrofolate cyclodeaminase (EC activity. This particular entry represents the dehydrogenase activity.

    Although the human protein represented here is actually trifunctional, the representative PDB structure only contains the dehydrogenase (D) and cyclohydrolase (C) domains, which have an overlapping active site (as determined by chemical modification and kinetic studies).

    5,10-methylene-tetrahydrofolate is converted to 5,10-methenyltetrahydrofolate. The mechanism involves hydride transfer from the substrate to NADP+.
    Catalytic Residues
    AA Uniprot Uniprot Resid PDB PDB Resid
    Gln P11586 100 1a4i 100
    Lys P11586 56 1a4i 56
    Asp P11586 125 1a4i 125

Reaction Parameters

There are no kinetic parameters information for this Enzyme

Associated Proteins

Protein name Organism
Bifunctional protein FolD 4, chloroplastic Mouse-ear cress
Bifunctional protein FolD 1/3 Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3)
Bifunctional protein FolD 1 Paracoccus denitrificans (strain Pd 1222)
Bifunctional protein FolD 2 Mouse-ear cress
Bifunctional protein MdtA Methylorubrum extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1)