Enzyme - Enoyl-[acyl-carrier-protein] reductase (NADH)

Alternative Name(s)
  • NADH-specific enoyl-ACP reductase.
  • Enoyl-ACP reductase.
  • NADH-enoyl acyl carrier protein reductase.

Catalytic Activity

2,3-saturated acyl-[ACP] + NAD(+) = a (2E)-enoyl-[ACP] + H(+) + NADH


There are no Cofactors for this Enzyme

Reaction Mechanism

    Enoyl ACP reductase catalyses the last step in fatty acid biosynthesis. Therefore it is a potential target for antibacterial agent development. It catalyses the NAD(P)H-dependent reduction of enoyl acyl carrier protein.

    The bacterial form of the enzyme is different from the human form as it exists as a free globular protein rather than a part of a multienzyme complex. EACPR's show homology, and similarity to hydroxysteroid dehydrogenase, and also beta-keto reductase, suggesting divergent evolution has played a role in the development of the pathway of lipid biosynthesis.

    The catalytic mechanism involves C3 of the substrate being subject to hydride attack by NADH, a bound cofactor. Formation of an enolate intermediate follows, which accepts a proton from Tyr 156. A role in transition state stabilisation through hydrogen bonding has also been suggested for Tyr 156. The enol product would then tautomerise to give the reduced acyl product. Tyr 156 therefore acts as the base that donates the proton to the enolate anion, and Lys 163 acts to stabilise the negatively charged transition state.
    Catalytic Residues
    AA Uniprot Uniprot Resid PDB PDB Resid
    Tyr P0AEK4 156 1qsg 159
    Lys P0AEK4 163 1qsg 166
    Step Components

    overall reactant used, proton transfer, overall product formed, native state of enzyme regenerated, keto-enol tautomerisation, cofactor used, hydride transfer, michael addition

    Step 1.

    C3 of the substrate is attacked by a hydride from NADH, forming an enolate and NAD. The oxygen of the enolate is protonated by Tyr156 resulting in an enol.

    Step 2.

    The enol tautomerises to form the product.


    The products of the reaction.

Reaction Parameters

There are no kinetic parameters information for this Enzyme

Associated Proteins

Protein name Organism
Enoyl-[acyl-carrier-protein] reductase [NADH] FabV Aeromonas salmonicida (strain A449)
Enoyl-[acyl-carrier-protein] reductase [NADH], chloroplastic Mouse-ear cress
Enoyl-[acyl-carrier-protein] reductase [NADH] 2 Ensifer meliloti
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI Escherichia coli (strain K12)
Enoyl-[acyl-carrier-protein] reductase [NADH] 2, chloroplastic Rice