Enzyme
1.1.1.49 - Glucose-6-phosphate dehydrogenase (NADP(+))
Alternative Name(s)
- Glucose-6-phosphate dehydrogenase.
- Glucose-6-phosphate 1-dehydrogenase.
- GPD.
- G6PD.
- G6PDH.
Catalytic Activity
D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + H(+) + NADPH
Cofactors
There are no Cofactors for this Enzyme
Reaction Mechanism
Glucose-6-phosphate dehydrogenase (G6PDH) is a ubiquitous protein, present in bacteria and all eukaryotic cell types. The enzyme catalyses the the first step in the pentose pathway: the conversion of glucose-6-phosphate to gluconolactone 6-phosphate in the presence of NADP, producing NADPH. The ubiquitous expression of the enzyme gives it a major role in the production of NADPH for the many NADPH-mediated reductive processes in all cells. Deficiency of G6PDH is a common genetic abnormality affecting millions of people worldwide. Many sequence variants, most caused by single point mutations, are known, exhibiting a wide variety of phenotypes.
The oxidation of glucose 6-phosphate by G6PD involves general base (His240) abstraction of a proton from the C1−OH, thereby allowing transfer of the hydride from C1 to the C4 position of the nicotinamide ring of the coenzyme.
Catalytic Residues
| AA | Uniprot | Uniprot Resid | PDB | PDB Resid |
|---|---|---|---|---|
| His | P11411 | 179 | 1dpg | 178 |
| Asp | P11411 | 178 | 1dpg | 177 |
| His | P11411 | 241 | 1dpg | 240 |
Step Components
overall product formed, proton transfer, overall reactant used, native state of enzyme regenerated, inferred reaction step, hydride transfer
Step 1.
His240 abstracts a proton from the substrate, eliminating a hydride that is added to NAD(P).
Step 2.
Inferred return step to deprotonate His240, ready for another reaction cycle.
Products.
The products of the reaction.
Reaction Parameters
There are no kinetic parameters information for this Enzyme
Associated Proteins
Citations
- G6PC1 and G6PC2 influence G6P flux but not HSD11B1 activity.
- Cell metabolism pathways involved in the pathophysiological changes of diabetic peripheral neuropathy.
- Evaluation of Cell Responses of Saccharomyces cerevisiae under Cultivation Using Wheat Bran as a Nutrient Resource by Analyses of Growth Activities and Comprehensive Gene Transcription Levels
- Apollo-NADP+ reveals in vivo adaptation of NADPH/NADP+ metabolism in electrically activated pancreatic β cells.
- Critical Role of 6-Phosphogluconate Dehydrogenase in TAp73-Mediated Cancer Cell Proliferation.
- Cytosolic malic enzyme and glucose-6-phosphate dehydrogenase modulate redox balance in NSCLC with acquired drug resistance.
- Rapid phosphorylation of glucose-6-phosphate dehydrogenase by casein kinase 2 sustains redox homeostasis under ionizing radiation.
- In Silico RNAseq and Biochemical Analyses of Glucose-6-Phosphate Dehydrogenase (G6PDH) from Sweet Pepper Fruits: Involvement of Nitric Oxide (NO) in Ripening and Modulation.
- PBX3 promotes pentose phosphate pathway and colorectal cancer progression by enhancing G6PD expression.
- Inferring mitochondrial and cytosolic metabolism by coupling isotope tracing and deconvolution.
- The pentose phosphate pathway in health and disease.