Enzyme

1.1.1.39 - Malate dehydrogenase (decarboxylating)

Alternative Name(s)
  • Malic enzyme.
  • Pyruvic-malic carboxylase.
  • NAD-malic enzyme.

Catalytic Activity

(S)-malate + NAD(+) = CO2 + NADH + pyruvate

Cofactors

There are no Cofactors for this Enzyme

Reaction Mechanism

There are no Reaction Mechanism for this Enzyme

Reaction Parameters

  • Kinetic Parameters
    Organism KM Value [mM] Substrate Comment
    Arabidopsis thaliana 10.3 (S)-malate pH 6.4, temperature not specified in the publication, NAD-ME1
    Rhodopseudomonas palustris 2900 NADP+ pH 7.2, 30°C, with (S)-malate
  • Temperature
    Organism Temperature Range Comment
    Brevundimonas diminuta 30 - 60 30°C: about 35% of maximal activity, 60°C: about 50% of maximal activity
  • pH
    Organism pH Range Comment
    Solanum tuberosum 5.8 - 7.6 pH 5.8: about 60% of maximal activity, pH 7.6: about 40% of maximal activity
    Clupea harengus 6 - 8 decarboxylation reaction, activity range
    Brevundimonas diminuta 6 - 8.5 about 40% of maximal activity at pH 6.0 and at pH 8.5
    Glossina morsitans 7 - 8.7 pH 7.0: about 30% of maximal activity, pH 8.7: about 45% of maximal activity

Associated Proteins

Protein name Organism
NAD-dependent malic enzyme 62 kDa isoform, mitochondrial Potato
NAD-dependent malic enzyme 65 kDa isoform, mitochondrial Prince-of-Wales feather
NAD-dependent malic enzyme 1, mitochondrial Mouse-ear cress
NAD-dependent malic enzyme 2, mitochondrial Mouse-ear cress
NAD-dependent malic enzyme 59 kDa isoform, mitochondrial Potato

Citations