Enzyme - Gluconate 2-dehydrogenase

Alternative Name(s)
  • 2-keto-D-gluconate reductase.
  • 2-ketogluconate reductase.

Catalytic Activity

D-gluconate + NADP(+) = 2-dehydro-D-gluconate + H(+) + NADPH


There are no Cofactors for this Enzyme

Reaction Mechanism

There are no Reaction Mechanism for this Enzyme

Reaction Parameters

  • Kinetic Parameters
    Organism KM Value [mM] Substrate Comment
    Aspergillus niger 0.85 2-dehydro-D-gluconate pH 8.0, temperature not specified in the publication
    Gluconobacter oxydans 9.34 2-oxo-D-gluconate pH 7.0, 25°C
  • Temperature
    Organism Temperature Range Comment
    Acetobacter ascendens 35 - 65 about half-maximal activity at 35°C and 65°C
    Acetobacter pasteurianus 40 - 55 about half-maximal activity at 40°C and 55°C
  • pH
    Organism pH Range Comment
    Acetobacter ascendens 4.5 - 8.5 about half-maximal activity at pH 4.5 and 8.5, reduction of ketogluconate
    Acetobacter pasteurianus 5 - 9.2 about half-maximal activity at pH 5.0 and 9.2, reduction of ketogluconate
    Pseudomonas plecoglossicida 8 no activity above

Associated Proteins

Protein name Organism
Probable 2-ketogluconate reductase Bacillus subtilis (strain 168)
2-ketogluconate reductase Gluconobacter oxydans (strain 621H)
Glyoxylate/hydroxypyruvate reductase B Escherichia coli (strain K12)
TrkA potassium uptake protein:D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain:D-isomer specific 2-hydroxyacid Brucella abortus (strain 2308)
GhrB protein Burkholderia sp. MSHR3999