Cofactors

There are no Cofactors for this Enzyme

Reaction Mechanism

2-dehydropantoate 2-reductase By Reference

Ketopantoate reductase (KPR), isolated from Escherichia coli, catalyses the reduction of ketopantoate by NADPH to form pantoate and NADP+. This is the second reaction in the pathway for the biosynthesis of pantothenate (vitamin B5). KPR is a member of the 6-phosphogluconate dehydrogenase superfamily. The reaction proceeds by a sequential ordered bi:bi kinetic mechanism, with NADPH binding first, followed by a conformational change and the binding of ketopantoate. After the reaction NADP+ dissociates first, followed by pantoate.




• Summary
• Step 1
• Step 2
• Products

The pro-S hydride of NADPH is transferred to the si face of the C2 carbonyl of ketopantoate. The developing alkoxide is protonated by Lys176 to form pantoate. Asn98 stabilises the active conformation of Lys176 and promotes the dissociation of NADP+.

Catalytic Residues

AA	Uniprot	Uniprot Resid	PDB	PDB Resid
Lys	P0A9J4	176	1yon	176

Step Components

overall reactant used, overall product formed, proton transfer, bimolecular nucleophilic addition, aromatic unimolecular elimination by the conjugate base, native state of enzyme regenerated, inferred reaction step, cofactor used, hydride transfer



Step 1.

Initial binding of NADPH induces a conformational change that facilitates ketopantoate binding. Nucleophilic addition of the C4 pro-S hydride of NADPH to the si face of ketopantoate then occurs. The developing alkoxide is protonated by Lys176.



Step 2.

In an inferred reaction step Lys176 is reprotonated to regenerate the native state of the enzyme.



Products.

The products of the reaction.

View Reaction Mechanisms in M-CSA

Reaction Parameters

There are no kinetic parameters information for this Enzyme

Associated Proteins

Citations

• Dynamically Regulating Glucose Uptake to Reduce Overflow Metabolism with a Quorum-Sensing Circuit for the Efficient Synthesis of d-Pantothenic Acid in Bacillus subtilis.
• Metabolic pathways that permit Mycobacterium avium subsp. hominissuis to transition to different environments encountered within the host during infection.
• Comparative analysis of genome-wide transcriptional responses to continuous heat stress in Pleurotus tuoliensis.
• Integration of Meta-Multi-Omics Data Using Probabilistic Graphs and External Knowledge.
• Comparative genomics of the proteostasis network in extreme acidophiles.
• Identification and Validation of Reference Genes for Expression Analysis in Nitrogen-Fixing Bacteria under Environmental Stress.
• Genome assembly of the chemosynthetic endosymbiont of the hydrothermal vent snail Alviniconcha adamantis from the Mariana Arc.
• In silico genomic analysis of the potential probiotic Lactiplantibacillus pentosus CF2-10N reveals promising beneficial effects with health promoting properties.
• Crystallographic and biophysical analyses of Pseudomonas aeruginosa ketopantoate reductase: Implications of ligand induced conformational changes in cofactor recognition.
• Functional characteristics of Svl3 and Pam1 that are required for proper cell wall formation in yeast cells.
• Manganese Modulates Metabolic Activity and Redox Homeostasis in Translationally Blocked Lactococcus cremoris, Impacting Metabolic Persistence, Cell Culturability, and Flavor Formation.