220.127.116.11 - Alcohol dehydrogenase
- Aldehyde reductase.
primary alcohol + NAD(+) = an aldehyde + H(+) + NADH
Zn(2+) or Fe cation.
An NAD+-dependent enzyme that catalyzes the oxidation of alcohols to aldehydes/ketones and that is also able to further oxidize aldehydes to their corresponding carboxylic acids. It is a zinc-independent protein which is a member of the short-chain dehydrogenases/reductases (SDR) family. It is known to be inhibited by 2,2,2-trifluoroethanol and pyrazole.
The NAD cofactor binds first, causing one of the active site waters to deprotonate Tyr152. Once the alcohol substrate has been bound, Tyr152 abstracts its hydroxyl proton, initiating the hydride transfer to NAD. The ketone product is released first, followed by the NADH.
|AA||Uniprot||Uniprot Resid||PDB||PDB Resid|
overall product formed, aromatic bimolecular nucleophilic addition, proton transfer, hydride transfer, cofactor used
Organism KM Value [mM] Substrate Comment Sphingomonas sp. A1 0.000015 D-sorbitol at pH 7.9 and 25°C Citrobacter sp. S-77 0.007 ethanol pH 7.0, 30°C Oenococcus oeni 0.01 1,2-propanediol pH 8.0, 60°C, recombinant enzyme Aeropyrum pernix 0.017 ethanol pH 8.0, 60°C Saccharomyces cerevisiae 0.018 propanal pH 7.0, 30°C
Organism Temperature Range Comment Flavobacterium frigidimaris 0 - 60 0°C: about 70% of maximal activity, about 45% of maximal activity Sporotrichum pulverulentum 10 - 60 10°C: about 65% of maximal activity, 50°C: about 40% of maximal activity Brassica napus 10 - 45 activity increases from 10°C to 45°C Oenococcus oeni 10 - 80 more than 40% of activity between 40°C and 70°C with all substrates. The enzyme is not capable of reducing acetaldehyde at 80°C, while remaining activity for alcohol oxidation, temperature optimum lies between 50°C to 60°C [Candida] maris 10 - 75 activity range, profile overview
Organism pH Range Comment Thermoplasma acidophilum 2 - 8 Methylorubrum extorquens 4 - 8 pH 4.0: about 35% of maximal activity, pH 8.0: about 70% of maximal activity [Candida] maris 4 - 8.5 oxidation reaction activity range, profile overview Candida parapsilosis 4 - 7.5 pH 4.0: about 80% of maximal activity, pH 7.5: about 60% of maximal activity Sulfolobus acidocaldarius 4.9 - 5.8 pH 4.9: about 25% of maximal activity, pH 5.8: about 40% of maximal activity, reduction reaction
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