Submitting environmental sequences

This covers the submission of data derived from the sequencing of DNA or RNA from organisms that have not been isolated and is typically focused on entire microbial communities, size fractionated organisms, enrichments cultures and flow sorted microbes. Both amplicon (metabarcoding, diversity survey) and shotgun (metagenomics/metatranscriptomics) based methods are included here.

Minimally, ENA aims to capture and present both raw data and identifications (taxonomic or functional) from environmental sequencing approaches. Additionally, intermediate information, such as mappings between raw reads and identifications and cluster information can also be captured.

Amplicon-based

Submitting reads and samples

Most 16S surveys use Next Generation Sequencing. The read domain Webin submission tool (see here) should be used to submit both raw sequencing reads and samples. Minimal sample description should be provided in this step. If you have assembled and annotated MIMARKS compliant 16S sequences, please contact datasubs@ebi.ac.uk to receive advise on their submission.

Shotgun-based

The ENA archives Next Generation Sequencing data from metagenomics and metatranscriptomics studies into the read domain. Comprehensive instructions on read data submission can be found here. These guidelines include both programmatic and interactive submissions.

Submitting metagenome assemblies

Metagenome assemblies (contigs, scaffolds and chromosomes) shall be submitted via the ENA (Meta)Genome Assembly Pipeline. A detailed guide on how to submit genomic and metagenomic assemblies is available here.

Sample contextual data

For all studies, but especially metagenomics and metatranscriptomics studies, it is essential that the read data files, study and sequencing experiment description are accompanied by minimal sample metadata information in order to ensure that the data originating from the study are reproducible, meaningful and can be sufficiently analysed.

Minimal reporting requirements for samples of metagenomics and metatrancriptomics studies are addressed in the MIxS data standard described here.

Metagenomic data analysis

Sequencing experiments correctly annotated as metagenomic and containing adequate sample contextual data are eligible for analysis by the EBI Metagenomics services. For further information please contact the EBI metagenomics team directly.

Latest ENA news

11 Oct 2017: Read data download issues resolved

Read data download issues previously affecting ftp.sra.ebi.ac.uk and fasp.sra.ebi.ac.uk services now resolved.

06 Oct 2017: ENA read data download issues

Issues with read data download from ftp.sra.ebi.ac.uk and fasp.sra.ebi.ac.uk

04 Oct 2017: ENA Release 133

Release 133 of ENA's assembled/annotated sequences now available