ID   X51522; SV 1; linear; genomic DNA; STD; PHG; 11624 BP.
XX
AC   X51522;
XX
DT   17-APR-1990 (Rel. 23, Created)
DT   14-NOV-2006 (Rel. 89, Last updated, Version 31)
XX
DE   Bacteriophage P4 complete DNA genome
XX
KW   alpha gene; beta gene; cI gene; cII gene; complete genome; delta gene;
KW   DNA primase; epsilon gene; gop gene; integrase; psu gene;
KW   satellite bacteriophage; sid gene; unidentified reading frame.
XX
OS   Enterobacteria phage P4
OC   Viruses; Caudovirales.
XX
RN   [1]
RC   revised by [4]  MAT
RA   Halling C.H.;
RT   ;
RL   Submitted (26-JAN-1990) to the INSDC.
RL   Halling C.H., University of California, c/o Richard Calendar, Department of
RL   Molecular and Cell Biology, Wendell M. Stanley Hall, Room 229, Berkeley, CA
RL   94720, USA.
XX
RN   [2]
RP   1-11624
RX   DOI; 10.1093/nar/18.6.1649.
RX   PUBMED; 2183201.
RA   Halling C., Calendar R., Christie G.E., Dale E.C., Deho G., Finkel S.,
RA   Flensburg J., Ghisotti D., Kahn M.L., Lane K.B., Lin C.S., Lindqvist B.H.,
RA   Pierson L.S., Six E.W., Sunshine M.G., Ziermann R.;
RT   "DNA sequence of satellite bacteriophage P4";
RL   Nucleic Acids Res. 18(6):1649-1649(1990).
XX
RN   [3]
RP   9468-9652
RX   PUBMED; 1987128.
RA   Van Bokkelen G.B., Dale E.C., Halling C., Calendar R.;
RT   "Mutational analysis of a bacteriophage P4 late promoter";
RL   J. Bacteriol. 173(1):37-45(1991).
XX
RN   [4]
RP   1-11624
RA   Halling C.H.;
RT   ;
RL   Submitted (26-JAN-1994) to the INSDC.
RL   Halling C.H., University of California, c/o Richard Calendar, Department of
RL   Molecular and Cell Biology, Wendell M. Stanley Hall, Room 229, Berkeley, CA
RL   94720, USA.
XX
RN   [5]
RP   1-11624
RX   DOI; 10.1006/viro.1995.1551.
RX   PUBMED; 7483254.
RA   Haggard-Liungquist E., Jacobsen E., Rishovd S., Six W.S., Nilssen O.,
RA   Sunshine M.G., Lindqvist B.H., Kim K.J., Barreiro V., Koonin E.V.,
RA   Calendar R.;
RT   "Bacteriophage P2: genes involved in baseplate assembly.";
RL   Virology 213(1):109-121(1995).
XX
DR   MD5; 5d2b37d233869a1a950d50d7b8ecf920.
DR   EuropePMC; PMC104256; 10438819.
DR   EuropePMC; PMC107552; 9748449.
DR   EuropePMC; PMC165782; 12867472.
DR   EuropePMC; PMC29674; 11139624.
DR   EuropePMC; PMC3177188; 21685451.
DR   EuropePMC; PMC94026; 10464191.
DR   RFAM; RF01394; isrK.
DR   RFAM; RF01695; C4.
XX
CC   See also <M11914>, <X16283>, <X05623>, <X05947>, <M27748> and
CC   <M29479>.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..11624
FT                   /organism="Enterobacteria phage P4"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:10680"
FT   precursor_RNA   179..>1718
FT                   /note="primary transcript of gop and beta"
FT   CDS             247..648
FT                   /transl_table=11
FT                   /note="gop gene product (AA 1-133)"
FT                   /db_xref="UniProtKB/Swiss-Prot:P13058"
FT                   /protein_id="CAA35894.1"
FT                   /translation="MLNHVYGLIGVVGTIITIATSFIRNQDISQWLLVCSGWLAALLIG
FT                   WFTHRTIKAISNNHTNVIKSNMEVINSHNESNQNLMAENKELIKELARTSEQKEKMESI
FT                   AAYLATQNPQINAMPRTASRPENIDSEAN"
FT   variation       541
FT                   /replace="t"
FT                   /note="in mutant gop-am24"
FT   CDS             651..1718
FT                   /transl_table=11
FT                   /note="beta gene product (AA 1-355)"
FT                   /db_xref="InterPro:IPR025683"
FT                   /db_xref="UniProtKB/Swiss-Prot:P13057"
FT                   /protein_id="CAA35895.1"
FT                   /translation="MKVYFENYSYYPALRTRSAEMTGLNNLSYENKKKILPLISLGKWP
FT                   RSEEIQVSLDKSLEVMSNLPFILDVTKDNSHHCASSFELLSPENGFKNWIEFCSRNDNI
FT                   IPVVQMPDSAKLRDISIQARVLEELKGSIAFRIRNLNTDINKTLTSLVSMNSPENAIVF
FT                   IDLGYIRGNVSAITAAAINSINQIRTEIPEAIISVLATSFPSSVTNFCRENGQSGYIDV
FT                   IERELHQNIGGSDVAIYGDHGSIHSVVYDNIIGRYVPRIDIALNDSWYFERRPGMNKEG
FT                   FIEAAKSILAEYPHYQREDSWGAAMIRNAAIGDIAGMGSPAKWIAVRVNLHLNKQIELS
FT                   EALQYGFDHDEEDLI"
FT   variation       763
FT                   /replace="c"
FT                   /note="in mutant beta-ts103"
FT   regulatory      complement(1720..1749)
FT                   /note="put. rho independent terminator of transcription of
FT                   cII"
FT                   /regulatory_class="terminator"
FT   variation       1734
FT                   /replace="t"
FT                   /note="in mutant ts37"
FT   CDS             complement(1746..2540)
FT                   /transl_table=11
FT                   /note="cII gene product (AA 1-264)"
FT                   /db_xref="UniProtKB/Swiss-Prot:P13059"
FT                   /protein_id="CAA35896.1"
FT                   /translation="MAELLREQEVKIALIEWLYKKGMLSDATIINEMVVANWSRRADLA
FT                   IANGSLQAFEIKSDFDSLKRLDGQLKIFKSSFEKVTIVCAPKFTSEVSKRVSSDVGVVE
FT                   FQSNNSSVRFKIIQKGRVCSRLNKFVYLGFLLKSELKDLLNQYGITFSNELHRDCLEVL
FT                   ASKLSLNKIREFVITSIKNRYHATSNDFLSRLHGNQEISINDLALLSKAKRRNNSLVKF
FT                   EFKACSEKSLNEYHKVDVDSIVRKYGDDVSYIPEKVLKRVVG"
FT   precursor_RNA   complement(<1746..2560)
FT                   /note="primary transcript of cII"
FT   variation       1868
FT                   /replace="t"
FT                   /note="in mutant cII-am3"
FT   CDS             complement(2607..3926)
FT                   /transl_table=11
FT                   /note="int gene product (AA 1-440)"
FT                   /db_xref="GOA:P08320"
FT                   /db_xref="InterPro:IPR002104"
FT                   /db_xref="InterPro:IPR010998"
FT                   /db_xref="InterPro:IPR011010"
FT                   /db_xref="InterPro:IPR013762"
FT                   /db_xref="InterPro:IPR025166"
FT                   /db_xref="InterPro:IPR038488"
FT                   /db_xref="UniProtKB/Swiss-Prot:P08320"
FT                   /protein_id="CAA35897.1"
FT                   /translation="MCPQMKLNARQVETAKPKDKTYKMADGGGLYLEVSAKGSKYWRMK
FT                   YRRPSDKKEDRLAFGVWPTVTLAQARAKRDEAKKLLVQGIDPKVVQKEARAENSGAYTF
FT                   EAIAREWHASNKRWSEDHRSRVLRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGK
FT                   HDVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTTKARHYPALPSSRFPEFLARL
FT                   AAYRGRVMTRIAVKLSLLTFVRSSELRFARWDEFDFDKSLWRIPAKREEIKGVRYSYRG
FT                   MKMKEEHIVPLSRQAMILLNQLKQISGDKELLFPGDHDATKVMSENTVNSALRAMGYDT
FT                   KTEVCGHGFRTMARGALGESGLWSDDAIERQLSHSERNNVRAAYIHTSEHLDERRLMMQ
FT                   WWADYLDMNRNKYISLMIIQNTKKYLNKNSYWLIFKMSVK"
FT   repeat_region   complement(3621..3632)
FT                   /note="attP arm sequence repeat"
FT   repeat_region   3915..3926
FT                   /note="attP arm sequence repeat"
FT   repeat_region   complement(3942..3953)
FT                   /note="attP arm sequence repeat"
FT   repeat_region   complement(3954..3965)
FT                   /note="attP arm sequence repeat"
FT   misc_feature    complement(4078..4097)
FT                   /note="attP (phage attachment site core sequence)"
FT   repeat_region   complement(4151..4162)
FT                   /note="attP arm sequence repeat"
FT   repeat_region   complement(4163..4174)
FT                   /note="attP arm sequence repeat"
FT   repeat_region   4291..4410
FT                   /note="crr 120bp direct repeat"
FT   misc_feature    4291..4590
FT                   /note="crr (cis required region for replication)"
FT   repeat_region   4314..4321
FT                   /note="ori type 1 repeat"
FT   repeat_region   4323..4330
FT                   /note="ori type 1 repeat"
FT   repeat_region   complement(4366..4373)
FT                   /note="ori type 1 repeat"
FT   repeat_region   complement(4384..4391)
FT                   /note="ori type 1 repeat"
FT   repeat_region   4398..4405
FT                   /note="ori type 1 repeat"
FT   repeat_region   4471..4590
FT                   /note="crr 120bp direct repeat"
FT   repeat_region   4494..4501
FT                   /note="ori type 1 repeat"
FT   repeat_region   4503..4510
FT                   /note="ori type 1 repeat"
FT   repeat_region   complement(4546..4553)
FT                   /note="ori type 1 repeat"
FT   repeat_region   complement(4564..4571)
FT                   /note="ori type 1 repeat"
FT   repeat_region   4578..4585
FT                   /note="ori type 1 repeat"
FT   regulatory      complement(4604..4628)
FT                   /note="talpha put. rho-independent terminator of
FT                   transcription of alpha"
FT                   /regulatory_class="terminator"
FT   CDS             complement(4636..6969)
FT                   /transl_table=11
FT                   /note="alpha gene product (AA 1-777)"
FT                   /db_xref="GOA:P10277"
FT                   /db_xref="InterPro:IPR004968"
FT                   /db_xref="InterPro:IPR006171"
FT                   /db_xref="InterPro:IPR013237"
FT                   /db_xref="InterPro:IPR014015"
FT                   /db_xref="InterPro:IPR014818"
FT                   /db_xref="InterPro:IPR027417"
FT                   /db_xref="InterPro:IPR034154"
FT                   /db_xref="InterPro:IPR036388"
FT                   /db_xref="InterPro:IPR036390"
FT                   /db_xref="PDB:1KA8"
FT                   /db_xref="UniProtKB/Swiss-Prot:P10277"
FT                   /protein_id="CAA35898.1"
FT                   /translation="MKMNVTATVSHALGHWPRILPALGIQVLKNRHQPCPVCGGSDRFR
FT                   FDDREGRGTWYCNQCGAGDGLKLVEKVFGVSPSDAAAKVAAVTGSLPPADPAVTTAAVD
FT                   ETDAARKNAAALAQTLMAKTRTGTGNAYLTRKGFPGRECRMLTGTHRAGGVSWRAGDLV
FT                   VPLYDDSGELVNLQLISADGRKRTLKGGQVRGTCHTLEGQNQAGKRLWIAEGYATALTV
FT                   HHLTGETVMVALSSVNLLSLASLARQKHPACQIVLAADRDLSGDGQKKAAAAADACEGV
FT                   VALPPVFGDWNDAFTQYGGEATRKAIYDAIRPPAESPFDTMSEAEFSAMSTSEKAMRIY
FT                   EHYGEALAVDANGQLLSRYENGVWKVLPPQDFARDVAGLFQRLRAPFSSGKVASVVDTL
FT                   KLIIPQQEAPSRRLIGFRNGVLDTQNGTFHPHSPSHWMRTLCDVDFTPPVDGETLETHA
FT                   PAFWRWLDRAAGGRAEKRDVILAALFMVLANRYDWQLFLEVTGPGGSGKSIMAEIATLL
FT                   AGEDNATSATIETLESPRERAALTGFSLIRLPDQEKWSGDGAGLKAITGGDAVSVDPKY
FT                   RDAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVIIHFPEQIAPQERDPQLKDKITREL
FT                   AVIVRHLMQKFSDPMLARSLLQSQQNSDEALNIKRDADPTFDFIGYLETLPQTSGMYMG
FT                   NASIIPRNYRKYLYHAYLAYMEANGYRNVLSLKMFGLGLPVMLKEYGLNYEKRHTKQGI
FT                   QTNLTLKEESYGDWLPKCDDPTTA"
FT   precursor_RNA   complement(<4636..8693)
FT                   /note="early transcript of cI through alpha"
FT   precursor_RNA   complement(<4636..9087)
FT                   /note="late delta regulated transcript of ORF88 through
FT                   alpha"
FT   variation       5032
FT                   /replace="a"
FT                   /note="in mutant alpha ts61"
FT   variation       5121
FT                   /replace="t"
FT                   /note="in mutant alpha am52"
FT   variation       6210
FT                   /replace="t"
FT                   /note="in mutant alpha am1"
FT   variation       6357
FT                   /replace="t"
FT                   /note="in mutant alpha am51"
FT   CDS             complement(6984..7304)
FT                   /transl_table=11
FT                   /note="ORF106 (AA 1-106)"
FT                   /db_xref="InterPro:IPR035317"
FT                   /db_xref="UniProtKB/Swiss-Prot:P68666"
FT                   /protein_id="CAA35899.1"
FT                   /translation="MKTPLPPVLRAALYRRAVACAWLTVCERQHRYPHLTLESLEAAIA
FT                   AELEGFYLRQHGEEKGRQIACALLEDLMESGPLKAAPSLSFLGLVVMDELCARHIKAPV
FT                   LH"
FT   CDS             complement(7440..7895)
FT                   /transl_table=11
FT                   /note="ORF151 (AA 1-151)"
FT                   /db_xref="UniProtKB/Swiss-Prot:P68664"
FT                   /protein_id="CAA35900.1"
FT                   /translation="MFDFPQPGEIYRSAGFPDVAVVGILEDGIPWEMPYRCPDIVWNPY
FT                   RRKFSILVRILADGRTTDIPLGRFLREFTCDRPDLFKRSPVNRHAVLKEMAGDPELQKW
FT                   REKYLDIYPQDTVPVSRAAPVAREWREIPRTEPDPETTPDNSYRNYL"
FT   CDS             complement(7888..8175)
FT                   /transl_table=11
FT                   /note="epsilon gene product (AA 1-95)"
FT                   /db_xref="UniProtKB/Swiss-Prot:P05463"
FT                   /protein_id="CAA35901.1"
FT                   /translation="MRNKKAPQTVSARHDAREHLSIEAYHKLNRASAVSRFVGGDLIHR
FT                   ELSGLHQLYIPHIFSYLNEDIDFVLNELKAKGLCRDFLAQQKDRGDRTHV"
FT   variation       8154
FT                   /replace="t"
FT                   /note="in mutant epsilon-am104"
FT   CDS             complement(8168..8584)
FT                   /transl_table=11
FT                   /note="cI gene product (AA 1-137)"
FT                   /db_xref="InterPro:IPR018880"
FT                   /db_xref="UniProtKB/Swiss-Prot:P05462"
FT                   /protein_id="CAA35902.1"
FT                   /translation="MMVWCVVSRADGIPCILPASAHYAAESMVAQAGQPPGWPVSCEAG
FT                   ILTPVWAIAIERENSGDSVICYSQEAAIMATTLTPSHPEFVFVFAAVRRADRHPRICML
FT                   RTVAGDERSARRSLVRDYVLSLAARLPVVEVSRA"
FT   variation       8255
FT                   /replace="gagaa"
FT                   /note="in mutant ash26 (double mutation)"
FT   variation       8433
FT                   /replace="a"
FT                   /note="in mutant ash-am29"
FT   variation       8434
FT                   /replace="c"
FT                   /note="T is C in mutant ash23"
FT   variation       8438..8442
FT                   /replace="ccccc"
FT                   /note="in mutants ash2, ash4, ash10 and ash29"
FT   variation       8438..8442
FT                   /replace="ccccc"
FT                   /note="in ash26 (double mutation)"
FT   variation       8443
FT                   /replace="c"
FT                   /note="in mutant ash-ts7"
FT   variation       8446
FT                   /replace="t"
FT                   /note="in mutant cI405"
FT   variation       8447
FT                   /replace="c"
FT                   /note="in mutant ash8"
FT   variation       8448
FT                   /replace="a"
FT                   /note="in mutant ash9"
FT   variation       8474
FT                   /replace="a"
FT                   /note="in mutant ash3"
FT   CDS             complement(8764..9030)
FT                   /transl_table=11
FT                   /note="ORF88 product (AA 1-88) (put. DNA-binding protein)"
FT                   /db_xref="GOA:P12552"
FT                   /db_xref="InterPro:IPR009061"
FT                   /db_xref="InterPro:IPR010260"
FT                   /db_xref="UniProtKB/Swiss-Prot:P12552"
FT                   /protein_id="CAA35903.1"
FT                   /translation="MQAVFSSPSPAPVTPLMPLPDITQERFLRVPEVMHLCGLSRSTIY
FT                   ELIRKGEFPPQVSLGGKNVAWLHSEVTAWMAGRIAGRKRGYDA"
FT   rep_origin      <9086..9809
FT                   /note="origin of DNA replication"
FT   variation       9099
FT                   /replace="t"
FT                   /note="in mutant vir1"
FT   repeat_region   9176..9185
FT                   /note="ori type 2 repeat"
FT   repeat_region   9186..9195
FT                   /note="ori type 2 repeat"
FT   repeat_region   9196..9205
FT                   /note="ori type 2 repeat"
FT   repeat_region   complement(9310..9317)
FT                   /note="ori type 1 repeat"
FT   repeat_region   complement(9320..9327)
FT                   /note="ori type 1 repeat"
FT   repeat_region   complement(9331..9338)
FT                   /note="ori type 1 repeat"
FT   repeat_region   9353..9360
FT                   /note="ori type 1 repeat"
FT   repeat_region   complement(9396..9403)
FT                   /note="ori type 1 repeat"
FT   repeat_region   complement(9406..9413)
FT                   /note="ori type 1 repeat"
FT   precursor_RNA   9557..11460
FT                   /note="late delta regulated transcript of sid through psu"
FT   CDS             9583..10317
FT                   /transl_table=11
FT                   /note="sid gene product (AA 1-244)"
FT                   /db_xref="UniProtKB/Swiss-Prot:P05461"
FT                   /protein_id="CAA35904.1"
FT                   /translation="MSDHTIPEYLQPALAQLEKARAAHLENARLMDETVTAIERAEQEK
FT                   NALAQADGNDADDWRTAFRAAGGVLSDELKQRHIERVARRELVQEYDNLAVVLNFERER
FT                   LKGACDSTATAYRKAHHHLLSLYAEHELEHALNETCEALVRAMHLSILVQENPLANTTG
FT                   HQGYVAPEKAVMQQVKSSLEQKIKQMQISLTGEPVLRLTGLSAATLPHMDYEVAGTPAQ
FT                   RKVWQDKIDQQGAELKARGLLS"
FT   variation       10288
FT                   /replace="t"
FT                   /note="in mutant sid-am1"
FT   CDS             10314..10814
FT                   /transl_table=11
FT                   /note="delta gene product (AA 1-166)"
FT                   /db_xref="GOA:P12551"
FT                   /db_xref="InterPro:IPR007684"
FT                   /db_xref="UniProtKB/Swiss-Prot:P12551"
FT                   /protein_id="CAA35905.1"
FT                   /translation="MIYCPSCGHVAHTRRAHFMDDGTKIMIAQCRNIYCSATFEASESF
FT                   FSDSKDSGMEYISGKQRYRDSLTSASCGMKRPKRMLVTGYCCRRCKGLALSRTSRRLSQ
FT                   EVTERFYVCTDPGCGLVFKTLQTINRFIVRPVTPDELAERLHEKQELPPVRLKTQSYSL
FT                   RLE"
FT   variation       10593
FT                   /replace="a"
FT                   /note="in mutant delta1 and delta2"
FT   variation       10611
FT                   /replace="t"
FT                   /note="in mutant delta6"
FT   variation       10614
FT                   /replace="t"
FT                   /note="in mutant delta4"
FT   variation       10692
FT                   /replace="g"
FT                   /note="in mutants org1, org2, org3 and org4"
FT   CDS             10888..11460
FT                   /transl_table=11
FT                   /note="psu gene product (AA 1-190)"
FT                   /db_xref="InterPro:IPR010006"
FT                   /db_xref="InterPro:IPR038395"
FT                   /db_xref="PDB:3RX6"
FT                   /db_xref="PDB:4DVD"
FT                   /db_xref="UniProtKB/Swiss-Prot:P05460"
FT                   /protein_id="CAA35906.1"
FT                   /translation="MESTALQQAFDTCQNNKAAWLQRKNELAAAEQEYLRLLSGEGRNV
FT                   SRLDELRNIIEVRKWQVNQAAGRYIRSHEAVQHISIRDRLNDFMQQHGTALAAALAPEL
FT                   MGYSELTAIARNCAIQRATDALREALLSWLAKGEKINYSAQDSDILTTIGFRPDVASVD
FT                   DSREKFTPAQNMIFSRKSAQLASRQSV"
FT   variation       10946
FT                   /replace="a"
FT                   /note="in mutant psu-am1"
FT   variation       11020
FT                   /replace="t"
FT                   /note="in mutant psu3"
FT   variation       11020
FT                   /replace="t"
FT                   /note="in mutant psu2"
XX
SQ   Sequence 11624 BP; 3008 A; 2864 C; 2893 G; 2859 T; 0 other;
     ggcgaggcgg ggaaagcacc gcgcgcaaac cgacaagtta gttaattatt tgtgtagtca        60
     aagtgccttc agtacatacc tcgttaatac attggagcat aatgaagaaa atctatggcc       120
     tatggtccaa aactgtcttt tttgatggca ctatcctgaa aaatatgcaa aaaatagatt       180
     gatgtaaggt ggttcttgtc agtgtcgcaa gatccttaag aattcgtggc atgagagagt       240
     taaaggatgc tgaatcatgt atatggatta attggcgttg ttgggaccat aatcactatt       300
     gctactagct tcatccgcaa ccaagatatt tcacaatggt tgctcgtttg ttctggatgg       360
     ttagctgctt tacttattgg atggtttaca cacagaacga ttaaagcaat tagcaacaac       420
     cacacgaatg ttataaaaag taatatggag gtaatcaaca gtcataatga atccaatcaa       480
     aacttgatgg ctgaaaacaa agaattgatt aaagaacttg caagaacttc agaacagaaa       540
     gagaagatgg aaagtatagc tgcttatctt gctactcaga acccccaaat taacgctatg       600
     ccaagaacag caagccgacc agaaaatatc gattcggagg ctaattaaat atgaaagtat       660
     attttgaaaa ttactcatac tatccagccc taagaacacg atcagcagaa atgaccggac       720
     tgaataattt atcatatgag aataagaaaa aaatattacc tttaatttct ttggggaaat       780
     ggcctcgctc ggaagaaata caggtatcgt tagataaaag tttagaagtg atgagtaatc       840
     tcccatttat tttggatgtc acaaaggata attctcatca ttgcgcgtca agcttcgaac       900
     tactctctcc tgagaatggt tttaaaaact ggatagagtt ttgttccaga aatgataata       960
     ttattccagt agtccagatg ccggattcag caaaacttag agatatatct atccaagcca      1020
     gggttttaga ggaacttaaa ggttcaattg catttagaat tagaaatcta aatacagaca      1080
     ttaataaaac cctgacgtca ctagtatcga tgaactctcc agaaaatgcc atagttttca      1140
     tagatttggg ctacattaga gggaatgtat ctgctataac agctgcagca ataaattcga      1200
     ttaatcagat tagaactgag attcctgaag ccataattag tgtgctagca acaagcttcc      1260
     ctagttcagt tacaaatttt tgccgtgaaa atggacagtc cggatatatt gatgttattg      1320
     agagagaatt gcatcaaaat attggtggaa gtgatgtcgc tatttatggc gatcatggtt      1380
     caattcattc agtagtgtat gacaacataa ttgggcgata tgttcctaga attgatatag      1440
     cgttaaatga ttcatggtat ttcgagcgcc gccctgggat gaataaagaa gggttcattg      1500
     aagctgcgaa atcaattttg gcagagtatc cacattatca aagggaggac tcttggggag      1560
     ctgcaatgat aagaaatgct gctatagggg atatagcagg tatgggaagt ccagctaaat      1620
     ggattgctgt tcgagtgaat ctacacttaa ataagcaaat tgagctatct gaagctctac      1680
     agtatggctt tgatcatgat gaggaagatc ttatctaata aaaaactagg gaaggttagc      1740
     cttccctagc ccacaaccct tttaagtact ttttccggaa tataggaaac atcatcgcca      1800
     tatttcctaa ctatagagtc aacatcaacc ttatggtatt catttaaaga tttctccgaa      1860
     caagccttaa actcaaactt aaccaaagaa ttattccttc ttttagcttt gctcaataag      1920
     gcaagatcgt ttatagatat ttcttgatta ccatggagtc tagataaaaa atcattagat      1980
     gtcgcatgat acctattttt aatagatgta atgacaaatt ctctaatctt gtttaaagat      2040
     aacttagatg ccaaaacctc aagacaatcc ctatgtagtt catttgaaaa tgtaattcca      2100
     tactgattaa gcagatcttt aagctcagac ttaagcaaaa aacccaaata aacaaattta      2160
     tttaagcgag agcaaaccct tcctttttga attattttaa agcgtacgct actattatta      2220
     gattgaaatt ctacaacgcc aacatctgat gaaacccgtt tagatacttc actggtaaat      2280
     tttggcgcgc acacaattgt aactttttcg aagctagatt taaaaatttt taactgacca      2340
     tccaagcgct taagagagtc aaaatcgctt tttatttcaa aagcttgtag gcttccattg      2400
     gcaatagcca aatcagctct tctgctccag ttagcaacaa ccatttcatt aatgatcgtt      2460
     gcatcactaa gcatgccttt tttatataac cattcaatta gggctatttt aacttcctgc      2520
     tctcgcaata actcagccat gttaatcaca ctcacttcac gttgattttt attatagcaa      2580
     ctaacaatta aaaactccag attatgttac ttaacactca ttttaaaaat caaccaataa      2640
     ctatttttat ttaagtattt ctttgtattt tgaataatca taaggctaat atatttatta      2700
     cggttcatat ccaaataatc agcccaccac tgcatcatta aacgccgctc atccaaatgc      2760
     tcggaggtat ggatatatgc cgcacgtaca ttattacgct ctgagtggct cagttgcctc      2820
     tctatcgcgt catcactcca taaccccgac tcccccaacg caccacgcgc catagtccta      2880
     aacccatgcc cacacacctc ggttttagta tcatagccca tcgcacgcaa tgcgctgttt      2940
     accgtgtttt cactcataac cttagttgcg tcatgatccc ccggaaaaag cagctcttta      3000
     tcaccactaa tctgctttaa ctggtttaat aaaatcatcg cctgtcgact aagcggaacg      3060
     atatgttcct ctttcatctt catgcctcgg tacgaataac gcacaccttt aatttcttct      3120
     cgctttgcag gtatacgcca aagagattta tcgaagtcga attcatccca acgtgcgaaa      3180
     cgtaattcac tggaacgcac aaaagttagc aaggaaagct tgaccgcaat ccgtgtcatt      3240
     acacggccac gatatgcagc aagacgtgca agaaactcag ggaatcggct agaaggtaaa      3300
     gcagggtaat gtcgcgcttt ggttgtcgat agcgcaccag ccatatcgct ggctggattt      3360
     gagtcgatgt aatcgttctg tacggcataa cgcataatgg ctgtgacgcg ctgttgcagg      3420
     cgctgagcga catcgtgttt gccactggca tcaacttttt taatcggggc taacaggtgg      3480
     ctggttttaa gctgccgaat gtcggacgaa ccgatatgag ggaatatata aagctcaaga      3540
     tagcgaagaa cgcgcgatcg atggtcttca ctccagcgct tgttactggc atgccattct      3600
     cgggcgatag cttcaaaagt atatgccccc gaattctcgg cccgagcttc tttttgtacg      3660
     acttttgggt ctatgccctg tactaaaagc tttttagctt catcgcgttt tgctcttgcc      3720
     tgagcaagcg tcacagtagg ccaaacacca aacgcaagac gatcctcttt tttgtcagag      3780
     ggacgtctgt atttcatgcg ccagtatttg gaacctttgg ccgaaacctc aagatacaaa      3840
     ccaccaccat cggccatttt gtaggttttg tcttttggct ttgcggtctc gacctgtctg      3900
     gcgttgagct tcatttgggg gcacatttct aatcgaagtt aagatgcccc caattatgcc      3960
     cccaatgata tccggatttc aacggacaac ctcggaagac gcaggacgaa aaaatcgctg      4020
     caagcattga ttttaaaggg atatttggac tttctcggat ggtcttggaa gtaagaatgg      4080
     tgccgaaggc cggactcaaa catcaaaata agttaatgat aaaaaacaaa taataaaaca      4140
     caacaatgaa atatgccccc ttttgtgccc ccactgtttt tctgaccaat ctattttcag      4200
     cccatcaata aatcggaaag ttaaatcatt tttaatcagt aagtttggat ccgtagctcg      4260
     gatccaaacc agtgcatctt ttatccacat aaaaaatttt ttttcgaaag aactgttcac      4320
     actgttcacc tttctgtttt ctccttttat ttcagagtga taggtggtga ataatgggtg      4380
     aagggtgaac attcgattct tcacctccgg cattctgccg atgtgactca taccggtgat      4440
     taatcctccg cactgaaatc actcaggaag aaaaaagttt tttttgattt gattgttcac      4500
     actgttcacc tttcgttttt ctcttttaat ttcagtgtga taacgggtga atatacggtg      4560
     aagggtgaac agtggattgt tcaccttcgg gggatatcgg gataaaaaaa gaccggcaga      4620
     tgccggtcag gtgggtcagg ctgttgtagg gtcgtcacat tttggcagcc agtcgccgta      4680
     gctttcctct ttcagcgtca ggttggtctg tatcccctgt ttggtatggc gtttctcgta      4740
     attcagtccg tattccttca gcatcaccgg cagccccagc ccgaacattt tcagactgag      4800
     tacattccgg tagccgtttg cctccatgta ggccagatag gcgtgataga ggtatttacg      4860
     gtaattgcgc gggatgatac tggcgttccc catatacatg ccgctggtct gcggcagggt      4920
     ttccagatag ccgataaaat caaacgtcgg gtcggcatcc cgtttgatgt tcagtgcctc      4980
     gtctgagttc tgctgggact gaagcagtga ccgggcgagc atcgggtcgc tgaacttctg      5040
     catcaggtga cgcacgatga ccgccagctc gcgggtgatt ttgtccttaa gctgcgggtc      5100
     gcgctcctgc ggggctatct gttccgggaa gtgaataatc acccgtcggc gtgacacgcc      5160
     gccgctgcgg tcggtgaagc gcatcgggtt attgttcacg gccagaatca ccgccgggat      5220
     gtgcgtggag tacgcatccc ggtatttcgg gtcaacggac accgcatcgc cgccggtgat      5280
     ggccttgagt ccggcaccgt cgccgctcca tttttcctgg tccggcaggc gtatcagtga      5340
     gaagccagtt aacgcggcac gttcacgcgg ggattccagc gtctcgatgg tggccgacgt      5400
     ggcgttatcc tccccggcca gcagggtggc tatttcggcc atgatacttt tgccgctgcc      5460
     gccgggaccg gtcacctcca gaaagagctg ccagtcgtag cggtttgcca gcaccataaa      5520
     cagtgcagcc agaatcacgt cgcgtttttc cgcacggcca ccggcggcac ggtcaagcca      5580
     gcgccagaag gcgggggcgt gggtttccag cgtttcaccg tccaccggcg gggtgaaatc      5640
     cacatcgcac agggtgcgca tccagtgtga cggactgtgc gggtggaacg tgccgttctg      5700
     cgtgtcgagc acgccgttac gaaagccaat caggcggcgg gagggggctt cctgctgcgg      5760
     aataatcagc ttcagggtat ccaccacgga ggccaccttc ccggaggaga acggcgcacg      5820
     cagacgctga aacagcccgg ccacatcccg ggcaaagtcc tgtggcggca gcaccttcca      5880
     gacaccattt tcatagcggg acagaagctg gccgttggca tcgaccgcga gcgcctcgcc      5940
     gtaatgctca tagatacgca tggccttttc gctggtactc atggcggaaa actccgcttc      6000
     gctcatggtg tcgaacgggc tttcagccgg tggccggatg gcatcgtaaa tggccttacg      6060
     ggtggcctcc ccgccgtact gcgtgaaggc atcattccag tcaccgaaga ccggcggcag      6120
     ggcaacaaca ccttcacacg catctgcggc tgcggcggct tttttctggc cgtcaccact      6180
     gaggtcacgg tcagcggcaa ggacaatctg acaggcgggg tgcttctgcc gggcaaggct      6240
     ggccagagaa aggaggttca cggaagaaag cgccaccatc accgtttcac cggtcaggtg      6300
     atgtacggta agtgcggtcg cgtatccctc cgctatccac agacgttttc cggcctgatt      6360
     ctgtccttca agggtgtgac aggtgcccct gacctgtccg cctttcaggg tgcgcttacg      6420
     gccgtcagca ctgattaact gaaggttaac cagttcgccg ctgtcgtcat acagtggcac      6480
     cacaaggtca ccggcgcgcc agctcacgcc accggctctg tgtgtgccgg tcagcatccg      6540
     gcattcccgg ccgggaaagc ccttgcgggt caggtaggcg ttaccggttc cggtacgggt      6600
     tttcgccatc agggtttgtg ccagtgcggc ggcgttcttc cgggcagcgt ctgtttcatc      6660
     aacggcggcg gtcgtcactg ccgggtcagc cggtggcagg ctgccggtca cggcagccac      6720
     ctttgcggcc gcgtcggacg gggaaacacc aaaaaccttt tcaaccagtt tcaggccgtc      6780
     accggcacca cactgattgc agtaccaggt gccgcgcccc tccctgtcat caaaacggaa      6840
     gcggtcactc ccgccacaga ccggacaggg ctgatgacgg ttcttcagca cctgaatccc      6900
     cagcgccggg agaatacgcg gccagtggcc gagcgcatgg ctgacggtgg cggttacgtt      6960
     cattttcatg gtgttgttct ccttcagtgc agtaccggcg cttttatgtg acgggcacag      7020
     agttcatcca tcacaaccag cccgagaaag gacagcgacg gcgcggcctt cagggggccg      7080
     gattccatta aatcttccag cagggcacag gctatctgac gccctttttc ctcaccgtgc      7140
     tggcgcagat aaaagccttc cagctcagcg gcgatggccg cctccagtga ctcaagggtg      7200
     agatgcgggt agcggtgctg acgttcgcac acggtcagcc aggcacaggc gacagcgcga      7260
     cggtaaaggg cagcgcgtaa gacgggcggt aagggtgttt tcatttgctt ttctccctgt      7320
     gacagatgac tgcattccgt gccggttgca ttaactgata aggcatatct gcgtctcctg      7380
     aagacgtgcg tatccctgcg cgaatacgca catttaattt ttcgggggtc gttttttaat      7440
     tacagataat tgcggtaact gttatccggg gtggtttccg ggtcaggctc cgtgcgggga      7500
     atttcccgcc attcccgcgc caccggtgct gcccggctga ccggaacagt gtcctgcggg      7560
     taaatatcca gatatttttc ccgccatttc tgtaattccg ggtctccggc catttctttc      7620
     agtaccgcat gccggtttac ggggctgcgt ttaaacaggt caggacggtc acaggtaaat      7680
     tcccgcagaa aacgccccag cgggatgtct gtggtgcgtc cgtcagcgag gatacgcaca      7740
     aggatactga atttacggcg gtacgggttc cagacaatgt ccgggcagcg gtacggcatt      7800
     tcccacggaa taccgtcttc cagaatgccg accacggcca catcgggaaa accggcagaa      7860
     cggtaaatct caccgggctg gggaaaatca aacatgcgtc ctgtctcccc ggtctttctg      7920
     ctgggcgaga aaatcgcggc acaggccttt ggctttcagc tcattcagca caaaatcaat      7980
     atcttcattc aggtagctga aaatatgtgg aatgtagagc tgatgcaggc cggagagttc      8040
     acggtgaatc aaatcacccc caacaaaccg ggatacggcg ctggcgcggt tgagcttatg      8100
     gtaagcctca atgctgaggt gttcacgggc gtcatgacgc gctgagacgg tctgaggggc      8160
     ttttttatta cgcacgggac acctccacca ccggcagacg ggcagcaagg gagagcacat      8220
     agtcacggac aagggaacgg cgggcactgc gttcatcacc ggcgacggtg cgaagcatac      8280
     agatacgggg atgacggtct gcgcgacgga cagccgcaaa cacaaagaca aattcagggt      8340
     gtgagggggt aagggttgta gccatgatgg cagcctcctg tgaatagcaa ataacgctat      8400
     cgccggagtt ctcacgctcg atggcgatag cccagacggg ggtgagaata ccggcttcac      8460
     aggataccgg ccagcccgga ggctgccccg cctgagctac cattgactct gcggcataat      8520
     gagcggacgc gggcaggatg cacggaatgc catctgcacg actgaccaca caccacacca      8580
     taatctggcg ctctgtggca ttgattgcga cacaaaaaaa gacgcgtggc gcgtcatatg      8640
     tcgcctgtga attgctcggg ttctcacgcc cggctgccga ttttgcggca ggcgaaaaac      8700
     tatatccgca aatgccggaa aaaggcaagc cagaaaaagg gagtttttgc agagcgggca      8760
     tcatcatgcg tcgtaccccc gtttgcgtcc ggcaatgcgt ccggccatcc atgcggtgac      8820
     ttcagagtgc agccaggcca catttttacc gccaagactc acctgcggcg gaaattcccc      8880
     cttacggatg agttcgtaga tggtcgagcg tgacaggccg cacaggtgca tcacttccgg      8940
     cacacgtaaa aaacgctcct gcgtgatgtc cggcagcggc atcagtggcg tcactggggc      9000
     gggagacggg gaagaaaaaa cagcttgcat cgggctacct cgttaatgtc catacagcac      9060
     cggataagtc cgtccggctt cgggtagcgc tttattttgt gaatattttc agcagacgca      9120
     acagggggga tttgttcagg ctgtcttaca atggctgtgt gttttttgtt catctccact      9180
     taaagtcatt taaagccact taaagcaatt tgtaattttt atagtgaaat acaaatcgtt      9240
     tcttcttatt cattcccggc gaattaataa aaacaaacag tagtaaacag cacaaaaagc      9300
     ccatcaacgg gtgaacagtg gtgaacagac ggtgaacagt cattactgcg attgttcacc      9360
     ctttaactta ctgtattact tatctttttt attaaggtga acagaggtga acagtaaaat      9420
     ataaaaaaac aaacagtaag ccggtttttc ctgcgacctt ttcctggctt gccggtctga      9480
     ggatgagtct cctgtgtcag ggctggcaca tctgcaatgc gtcgtgttgt tgtccggtgt      9540
     acgtcacaat tttcttaacc tgaagtgacg aggagccgga aaatgtctga ccacactatc      9600
     cctgaatatc tgcaacccgc actggcacaa ctggaaaagg ccagagccgc ccatcttgag      9660
     aacgcccgcc tgatggatga gaccgtcacg gccattgaac gggcagagca ggaaaaaaat      9720
     gcgctggcgc aggccgacgg aaacgacgct gacgactggc gcacggcctt tcgtgcagcc      9780
     ggtggtgtcc tgagcgacga gctgaaacag cgccacattg agcgcgtggc acgccgggag      9840
     ctggtacagg aatatgacaa tctggccgtg gtgctgaatt tcgaacgtga acgcctgaaa      9900
     ggggcgtgtg acagcacggc caccgcctac cggaaggcac atcatcacct tctgagtctg      9960
     tatgcagagc atgagctgga acacgccctg aatgaaacct gtgaggcgct tgtccgggca     10020
     atgcatctga gcattctggt acaggaaaat ccgctcgcca acaccaccgg ccatcagggc     10080
     tacgtcgcac cggaaaaggc tgtcatgcag caggtgaaat catcgctgga acagaaaatt     10140
     aaacagatgc aaatcagcct caccggcgag ccggttctcc ggctgaccgg actgtcagcg     10200
     gcaacactcc cgcacatgga ttatgaggtg gcaggcacac cggcacagcg caaggtgtgg     10260
     caggacaaaa tagaccagca gggagcagag cttaaggcca gagggctgct gtcatgattt     10320
     actgtccgtc gtgtggacat gttgctcaca cccgtcgcgc acatttcatg gacgatggca     10380
     ccaagataat gattgcacag tgccggaata tttattgctc tgcgacattt gaagcgagtg     10440
     aaagcttttt ctctgacagt aaagattcag gaatggaata catttcaggc aaacagagat     10500
     accgcgattc actgacgtca gcctcctgcg gtatgaaacg cccgaaaaga atgcttgtta     10560
     ccggatattg ttgtcggaga tgtaaaggcc ttgcactgtc aagaacatcg cggcgtctgt     10620
     ctcaggaagt caccgagcgt ttttatgtgt gcacggatcc gggctgtggt ctggtgttta     10680
     aaacgcttca gaccatcaac cgcttcattg tccgcccggt cacgccggac gaactggcag     10740
     aacgcctgca tgaaaaacag gaactgccgc cagtacggtt aaaaacacaa tcatattcgc     10800
     tgcgtctgga atgagggctg ccggttaaca ccggccgtcg ccgcacaccg tatttttatt     10860
     cttcagcatg atgagaaaga gataacgatg gaaagcacag ccttacagca ggcctttgac     10920
     acctgtcaga ataacaaagc agcatggctg caacgcaaaa atgagctggc agcggccgaa     10980
     caggaatatc tgcggcttct gtcaggagaa ggcagaaacg tcagtcgcct ggacgaatta     11040
     cgcaatatta tcgaagtcag aaaatggcag gtgaatcagg ccgccggtcg ttatattcgt     11100
     tcgcatgaag ccgttcagca catcagcatc cgcgaccggc tgaatgattt tatgcagcag     11160
     cacggcacag cactggcggc cgcactggca ccggagctga tgggctacag tgagctgacg     11220
     gccattgccc gaaactgtgc catacagcgt gccacagatg ccctgcgtga agcccttctg     11280
     tcctggcttg cgaagggtga aaaaattaat tattccgcac aggatagcga cattttaacg     11340
     accatcggat tcaggcctga cgtggcttcg gtggatgaca gccgtgaaaa attcacccct     11400
     gcgcagaaca tgattttttc gcgtaaaagt gcgcaactgg catcacgtca gtcagtgtaa     11460
     aattccccga aaatccgccc gtttttactg aaaaaagcca tgcatcgata aggtgcatgg     11520
     ctttgcatgc gttttcctgc ctcattttct gcaaaccgcg ccattcccgg cgcggtctga     11580
     gcgtgtcagt gcaactgcat taaaaccgcc ccgcaaagcg ggcg                      11624
//