ID   X04466; SV 1; circular; genomic DNA; STD; PRO; 9957 BP.
XX
AC   X04466; J01558-J01562; K02333; M16195; M17497; M23861; M38599; V00377;
AC   X00141; X00223; X01287;
XX
DT   19-SEP-1987 (Rel. 13, Created)
DT   23-OCT-2008 (Rel. 97, Last updated, Version 18)
XX
DE   Bacteriocinogenic plasmid CloDF13 complete nucleotide sequence
XX
KW   bacteriocin; ccl gene; cex gene; cim gene; circular; cloacin; cun gene;
KW   dpi gene; immunity protein; inverted repeat; K-L operon; mobA gene;
KW   mobB gene; origin of replication; plasmid; rpi gene; SOS-box.
XX
OS   Escherichia coli
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OG   Plasmid CloDF13
XX
RN   [1]
RP   1-9957
RX   DOI; 10.1016/0147-619X(86)90072-7.
RX   PUBMED; 3749334.
RA   Nijkamp H.J., de Lang R., Stuitje A.R., den Elsen P.J., Veltkamp E.,
RA   van Putten A.J.;
RT   "The complete nucleotide sequence of the bacteriocinogenic plasmid
RT   CloDF13";
RL   Plasmid 16(2):135-160(1986).
XX
RN   [2]
RP   2994-5063
RX   DOI; 10.1093/nar/11.24.8791.
RX   PUBMED; 6324101.
RA   van Den Elzen P.J.M., Hakkaart M.J.J., van Putten A.J., Walters H.H.B.,
RA   Veltkamp E., Nijkamp H.J.H.;
RT   "Structure and regulation of gene expression of a Clo DF13 plasmid DNA
RT   region involved in plasmid segregation and incompatibility.";
RL   Nucleic Acids Res. 11(24):8791-8808(1983).
XX
RN   [3]
RP   1126-1467
RX   DOI; 10.1093/nar/8.19.4349.
RX   PUBMED; 6253914.
RA   Den Elzen P.J., Gaastra W., Spelt C.E., de Graaf F.K., Veltkamp E.,
RA   Nijkamp H.J.;
RT   "Molecular structure of the immunity gene and immunity protein of the
RT   bacteriocinogenic plasmid Clo DF13";
RL   Nucleic Acids Res. 8(19):4349-4363(1980).
XX
RN   [4]
RP   1-296,9355-9957
RX   DOI; 10.1007/BF00392189.
RX   PUBMED; 6336327.
RA   Snijders A., van Putten A.J., Veltkamp E., Nijkamp H.J.;
RT   "Localization and nucleotide sequence of the bom region of Clo DF13";
RL   Mol. Gen. Genet. 192(3):444-451(1983).
XX
RN   [5]
RP   932-1082
RX   DOI; 10.1007/BF00270635.
RX   PUBMED; 6276683.
RA   Hakkaart M.J.J., Veltkamp E., Nijkamp H.J.J.;
RT   "Protein H encoded by plasmid Clo DF13 involved in lysis of the bacterial
RT   host. I. Localisation of the gene and identification and subcellular
RT   localisation of the gene H product";
RL   Mol. Gen. Genet. 183(2):318-325(1981).
XX
RN   [6]
RP   4405-4905
RX   DOI; 10.1016/0092-8674(84)90090-4.
RX   PUBMED; 6319008.
RA   Hakkaart M.J.J., van Den Elzen P.J.M., Veltkamp E., Nijkamp H.J.J.;
RT   "Maintenance of multicopy plasmid Clo DF13 in E. coli cells: evidence for
RT   site-specific recombination at parB";
RL   Cell 36(1):203-209(1984).
XX
RN   [7]
RX   DOI; 10.1093/nar/11.8.2465.
RX   PUBMED; 6344017.
RA   Den Elzen P.J., Walters H.H., Nijkamp H.J.;
RT   "Molecular structure and function of the bacteriocin gene and bacteriocin
RT   protein of plasmid Clo DF13";
RL   Nucleic Acids Res. 11(8):2465-2477(1983).
XX
RN   [8]
RX   DOI; 10.1016/0147-619X(81)90078-0.
RX   PUBMED; 6164067.
RA   Veltkamp E., Stuitje A.R.;
RT   "Replication and structure of the bacteriocinogenic plasmids Clo DF13 and
RT   CoI E1";
RL   Plasmid 5(1):76-99(1981).
XX
RN   [9]
RX   DOI; 10.1093/nar/8.7.1459.
RX   PUBMED; 6253936.
RA   Stuitje A.R., Veltkamp E., Maat J., Heyneker H.L.;
RT   "The nucleotide sequence surrounding the replication origin of the cop3
RT   mutant of the bacteriocinogenic plasmid Clo DF13";
RL   Nucleic Acids Res. 8(7):1459-1473(1980).
XX
DR   MD5; e2d6438b192fc1745c1ebad659242338.
DR   RFAM; RF00106; RNAI.
XX
CC   16 identified biological functions can be assigned to the primary
CC   structure of the CloDF13 DNA.  The functions include those of
CC   eight protein encoding genes (cex gene for protein H; cim gene
CC   for immunity protein=protein E; ccl gene for cloacin=protein A;
CC   cun gene for protein K; dpi gene for L-protein; rpi gene for
CC   protein D; mobB gene for protein C; mobA gene for protein B), two
CC   untranslated RNA species RNA I; RNA II) and six DNA sites.  For
CC   convenience the CloDF13 genome has been devided into 5 defined
CC   regions: region I (origin of vegetative replication, priming and
CC   control of replication, type I incompatibility),region II
CC   (cloacin DF13, cloacin immunity, cloacin release, cloacin operon
CC   control), region III (double-stranded DNA-phage interaction, type
CC   II incompatibility multimer resolution), rergion IV (inhibition
CC   of male specific RNA phages and transfer of Flac), and region V
CC   (mobility proteins, basis of mobility).
CC   
CC   Data kindly reviewed (25-MAY-1984) by A.J. van Putten
CC   Data kindly reviewed by Hakkaart M.J.J.
CC   Data kindly reviewed (26-SEPT-1987) by Van Putten
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..9957
FT                   /organism="Escherichia coli"
FT                   /plasmid="CloDF13"
FT                   /strain="K12 P678-54"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:562"
FT   misc_feature    complement(<1..147)
FT                   /note="bom region; basis of mobilisation (circular
FT                   molecule)"
FT   regulatory      complement(63..68)
FT                   /note="-10 region (bom 1)"
FT                   /regulatory_class="promoter"
FT   regulatory      complement(83..88)
FT                   /note="-35 region (bom 1)"
FT                   /regulatory_class="promoter"
FT   regulatory      complement(85..90)
FT                   /note="-10 region (bom 2)"
FT                   /regulatory_class="promoter"
FT   misc_feature    105..119
FT                   /note="pot. interaction site for cAMP/-CRP receptor protein
FT                   complex"
FT   regulatory      complement(109..114)
FT                   /note="-35 region (bom 2)"
FT                   /regulatory_class="promoter"
FT   misc_feature    282..282
FT                   /note="origin of vegetative replication"
FT   regulatory      733..738
FT                   /note="-35 region (RNA I)"
FT                   /regulatory_class="promoter"
FT   regulatory      757..762
FT                   /note="-10 region (RNA I)"
FT                   /regulatory_class="promoter"
FT   repeat_region   complement(845..853)
FT                   /note="inverted repeat A'"
FT   regulatory      complement(845..866)
FT                   /note="put. stem-loop structure; put. transcription
FT                   terminator (RNA I)"
FT                   /regulatory_class="terminator"
FT   repeat_region   complement(858..866)
FT                   /note="inverted repeat A"
FT   regulatory      complement(885..890)
FT                   /note="-10 region (RNA II)"
FT                   /regulatory_class="promoter"
FT   repeat_region   complement(902..909)
FT                   /note="inverted repeat B'"
FT   regulatory      complement(902..924)
FT                   /note="put. stem-loop structure; put. transcription
FT                   terminator (cex gene)"
FT                   /regulatory_class="terminator"
FT   regulatory      complement(908..913)
FT                   /note="-35 region (RNA II)"
FT                   /regulatory_class="promoter"
FT   repeat_region   complement(917..924)
FT                   /note="inverted repeat B"
FT   CDS             complement(933..1082)
FT                   /transl_table=11
FT                   /note="protein H (AA 1-49)"
FT                   /db_xref="GOA:P02987"
FT                   /db_xref="InterPro:IPR003059"
FT                   /db_xref="UniProtKB/Swiss-Prot:P02987"
FT                   /protein_id="CAA28145.1"
FT                   /translation="MKKAKAIFLFILIVSGFLLVACQANYIRDVQGGTVAPSSSSELTG
FT                   IAVQ"
FT   regulatory      complement(1086..1092)
FT                   /note="rRNA binding site (cex gene)"
FT                   /regulatory_class="ribosome_binding_site"
FT   regulatory      complement(1153..1157)
FT                   /note="rho-dependent transcription terminator (cim gene)"
FT                   /regulatory_class="terminator"
FT   CDS             complement(1167..1424)
FT                   /transl_table=11
FT                   /note="immunity protein (AA 1-85)"
FT                   /db_xref="GOA:P02986"
FT                   /db_xref="InterPro:IPR003063"
FT                   /db_xref="InterPro:IPR036528"
FT                   /db_xref="UniProtKB/Swiss-Prot:P02986"
FT                   /protein_id="CAA28146.1"
FT                   /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
FT                   NGWFDVEKPWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
FT   regulatory      complement(1430..1433)
FT                   /note="rRNA binding site (cim)"
FT                   /regulatory_class="ribosome_binding_site"
FT   CDS             complement(1434..3119)
FT                   /transl_table=11
FT                   /note="g cloacin(AA 1-561)"
FT                   /db_xref="GOA:P00645"
FT                   /db_xref="InterPro:IPR009105"
FT                   /db_xref="InterPro:IPR016128"
FT                   /db_xref="InterPro:IPR024566"
FT                   /db_xref="InterPro:IPR024575"
FT                   /db_xref="InterPro:IPR036302"
FT                   /db_xref="InterPro:IPR036725"
FT                   /db_xref="UniProtKB/Swiss-Prot:P00645"
FT                   /protein_id="CAA28147.1"
FT                   /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
FT                   PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
FT                   ATPGAEGPALSFSGDALSSAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
FT                   IMTSLPADTVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
FT                   TKRPGVFSVSIPGLPALQVSVPKGVPAAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
FT                   FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
FT                   EDKNITTLNGRITSTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
FT                   VRYLDKDIQNQNAKLKAAQASLNAMNDALSRDKAALSGAMESRKQKEKKAKEAENKLNE
FT                   EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
FT                   EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
FT   regulatory      complement(3128..3131)
FT                   /note="rRNA binding site (ccl gene; cloacin)"
FT                   /regulatory_class="ribosome_binding_site"
FT   misc_feature    3165..3174
FT                   /note="SOS-box, pot. lexA bindig site"
FT   regulatory      complement(3187..3192)
FT                   /note="-10 region (cloacin operon)"
FT                   /regulatory_class="promoter"
FT   misc_feature    3211..3234
FT                   /note="cII, pot. interaction site for cAMP-CRA"
FT   regulatory      complement(3216..3221)
FT                   /note="-35 region (cloacin operon)"
FT                   /regulatory_class="promoter"
FT   misc_feature    3324..3348
FT                   /note="cI, pot. interaction site for cAMP-CRA rec. protein
FT                   complex"
FT   regulatory      3327..3333
FT                   /note="-35 region (K-L operon)"
FT                   /regulatory_class="promoter"
FT   regulatory      3350..3355
FT                   /note="-10 region (K-L operon)"
FT                   /regulatory_class="promoter"
FT   regulatory      3802..3806
FT                   /note="rRNA binding site (cun gene)"
FT                   /regulatory_class="ribosome_binding_site"
FT   CDS             3813..4379
FT                   /transl_table=11
FT                   /note="K protein (AA 1-188)"
FT                   /db_xref="UniProtKB/Swiss-Prot:P02988"
FT                   /protein_id="CAA28148.1"
FT                   /translation="MDKRTTLVALVSIIFFTSGCDDQKKGNGNAESTQITKNDITTQLK
FT                   LDKTQQPDDIEKKIKEIQANSTPEKIKKFNELKDDDPKILRESVVSEIKKKLPLLVDEA
FT                   TLMTDVSTDGGTFSYKYVTKGISASTMESDVWKDAMQKNIKNSYCSDDARLKVFRELFP
FT                   EGVIYNYYLSDKLIYTYKALPSICS"
FT   regulatory      4402..4416
FT                   /note="put. stem-loop structure; put. transcription
FT                   terminator (cun gene)"
FT                   /regulatory_class="terminator"
FT   repeat_region   4402..4409
FT                   /note="inverted repeat C"
FT   repeat_region   4410..4416
FT                   /note="inverted repeat C'"
FT   regulatory      4453..4457
FT                   /note="rRNA binding site (dpi gene)"
FT                   /regulatory_class="ribosome_binding_site"
FT   CDS             4465..4728
FT                   /transl_table=11
FT                   /note="L protein (AA 1-87)"
FT                   /db_xref="UniProtKB/Swiss-Prot:P02989"
FT                   /protein_id="CAA28149.1"
FT                   /translation="MAAYTSETAKFLQSVSSDQTWWKSRYNPGDSVPHSGIYRCTVCGK
FT                   EITSNGNDPFPPQNHHQHSQQQAIKWQLIVRTDTKGDRFGIK"
FT   repeat_region   4799..4819
FT                   /note="inverted repeat D"
FT   regulatory      4799..4819
FT                   /note="put. stem-loop structure; put. transcription
FT                   terminator (dpi gene)"
FT                   /regulatory_class="terminator"
FT   repeat_region   4812..4819
FT                   /note="inverted repeat D'"
FT   regulatory      6267..6272
FT                   /note="-35 region (rpi 1)"
FT                   /regulatory_class="promoter"
FT   regulatory      6298..6303
FT                   /note="-10 region (rpi 1)"
FT                   /regulatory_class="promoter"
FT   regulatory      6321..6367
FT                   /note="-35 region (rpi 2)"
FT                   /regulatory_class="promoter"
FT   regulatory      6383..6388
FT                   /note="-10 region (rpi 2)"
FT                   /regulatory_class="promoter"
FT   regulatory      6523..6527
FT                   /note="rRNA binding site (rpi gene)"
FT                   /regulatory_class="ribosome_binding_site"
FT   CDS             6536..6970
FT                   /transl_table=11
FT                   /note="D protein (AA 1-144)"
FT                   /db_xref="UniProtKB/Swiss-Prot:P13809"
FT                   /protein_id="CAA28150.1"
FT                   /translation="MAFSSLLSRSSYIPMDFIIARCRARSTKQKTYAPVCQASTTISSR
FT                   CSLVGMMQRSPSRSSASSINTMYSAIIPHSVVINLKHANSRSRSSSAVLRASPVCGLTI
FT                   SSTDRIARCTLTCVARSGQMYQISSMPFARRLNRYPDTTS"
FT   regulatory      6988..7016
FT                   /note="put. stem-loop structure; put. transcription
FT                   terminator (rpi/mobB)"
FT                   /regulatory_class="terminator"
FT   repeat_region   6988..6997
FT                   /note="inverted repeat E"
FT   repeat_region   7008..7016
FT                   /note="inverted repeat E'"
FT   CDS             complement(7061..7507)
FT                   /transl_table=11
FT                   /note="C protein (AA 1-148)"
FT                   /db_xref="GOA:P08097"
FT                   /db_xref="UniProtKB/Swiss-Prot:P08097"
FT                   /protein_id="CAA28151.1"
FT                   /translation="MILRGCTMALERYNVSQPKRQAERGENTADPALAAGRACSTAELV
FT                   ARRLGIAAVQPVYRFLDTWWRKGCWSGEITRLTAVRSACGADAARGGVLVRRGRAADGH
FT                   YSVSCRRGVCAQLPRTGWPFELLRLAMEGNSRGAPAGGTCTVLP"
FT   regulatory      complement(7511..7514)
FT                   /note="rRNA binding site (mobB gene)"
FT                   /regulatory_class="ribosome_binding_site"
FT   repeat_region   complement(7611..7616)
FT                   /note="inverted repeat F'"
FT   regulatory      complement(7611..7630)
FT                   /note="put. stem-loop structure; put. transcription
FT                   terminator (mob gene) site"
FT                   /regulatory_class="terminator"
FT   repeat_region   complement(7625..7630)
FT                   /note="inverted repeat F"
FT   CDS             complement(7649..9238)
FT                   /transl_table=11
FT                   /note="B protein (AA 1-529)"
FT                   /db_xref="GOA:P08098"
FT                   /db_xref="InterPro:IPR016387"
FT                   /db_xref="InterPro:IPR027417"
FT                   /db_xref="UniProtKB/Swiss-Prot:P08098"
FT                   /protein_id="CAA28152.1"
FT                   /translation="MGLSHKERREYGRSYKRDNNRTAPFSCWPRWIRPGGWSGHGGMSG
FT                   TTRTGVTARLAFWGGWLSPCLSRAGEKPSCVRTSTRTQIITCCSRLTCSGLPGARAGWL
FT                   SGLCWRGSGVLLLDGHFAAADEAVNRKLNRRAGAQPATGEMTDVRQPAFRGAGTVNALA
FT                   RFFHGRGPETAGVFLGKDEQGEPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRSVR
FT                   DFGDAVVYFDQRGPVAALLPAHCPNFTLLDLRPGKPAQLNLFRDLDQYALKNLLVAGFN
FT                   LSETGHVADHYRISEQKAAKLIAEQVSAGGKHSAGAGGGVRAAGGPEKGREGADHQAGE
FT                   RGRPERPADGQRHRRGGDNQRRRLLYVIGSMDDEAVIRVQKMLFARCAQIIIARDEFRR
FT                   WPHASIMLDEIKYLLSKYVLNALGTVRSRDCNLRLAHHAGRLRAAGQDLPADFVKTTVL
FT                   DNTPIRWFYRAASQESRSGAGQTGEIRVDVERRGPAGRRGTWSISAGTASSRRSRPLFD
FT                   VNTLQHLLTGSR"
FT   regulatory      complement(9245..9249)
FT                   /note="rRNA binding site (mobA gene)"
FT                   /regulatory_class="ribosome_binding_site"
FT   regulatory      complement(9398..9403)
FT                   /note="-10 region (mobility operon)"
FT                   /regulatory_class="promoter"
FT   regulatory      complement(9419..9424)
FT                   /note="-35 region (mobility operon)"
FT                   /regulatory_class="promoter"
FT   misc_feature    complement(9775..>9957)
FT                   /note="bom region; basis of mobilisation (circular
FT                   molecule)"
XX
SQ   Sequence 9957 BP; 2332 A; 3018 C; 2399 G; 2208 T; 0 other;
     aacgtaaaat gttcagcgaa aaaccgacat ggttcaccta tcctgataat tgatcgtcag        60
     gcaatagaaa gacgtaatca ggggacaata tcccacatca gcgcgtccgg tgaccgtctt       120
     gcaggtgccg gggaaattgc gcctcctcgc tcactcggtc gctacgctcc ggccgtgaga       180
     ctgcggcggg cgctgcggac acatacaaag ttacccacag attccgtgta taagcagggg       240
     actaacatgt gaggcaaaac agcagggccg cgccggtggc gtttttccat aggctccgcc       300
     ctcctgccag agttcacata aacagacgct tttccggtgc atctgtggga gccgtgaggc       360
     tcaaccatga atctgacagt acgggcgaaa cccgacagga cttaaagatc cccaccgttt       420
     ccggcgggtc gctccctctt gcgctctcct gttccgaccc tgccgtttac cggatacctg       480
     ttccgccttt ctcccttacg ggaagtgtgg cgctttctca tagctcacac actggtatct       540
     cggctcggtg taggtcgttc gctccaagct gggctgtaag caagaactcc ccgttcagcc       600
     cgactgctgc gccttatccg gtaactgttc acttgagtcc aacccggaaa agcacggtaa       660
     aacgccactg gcagcagcca ttggtaactg ggagttcgca gaggatttgt ttagctaaac       720
     acgcggttgc tcttgaagtg tgcgccaaag tccggctaca ctggaaggac agatttggtt       780
     gctgtgctct gcgaaagcca gttaccacgg ttaagcagtt ccccaactga cttaaccttc       840
     gatcaaacca cctccccagg tggttttttc gtttacaggg caaaagatta cgcgcagaaa       900
     aaaaggatct caagaagatc ctttgatctt ttctactgaa ccgcgatccc cgtcagttca       960
     gaggaggacg atggtgccac cgttccaccc tgaacatccc ggatatagtt tgcctgacat      1020
     gcgaccagaa gaaagcctga aactatcaat ataaaaagaa aaatagcctt tgcctttttc      1080
     atataccttc cttgtattca gaggcttcgg cattcatccg tgaacaccgg aagcctgtat      1140
     ttcaactcat ccttgataac ttttgcttac cagttaccat cccggtaaac aaaggataca      1200
     aagtaatcaa atttactaat atcgattaca tttttaaagt gtggctgtaa tatcgaaacc      1260
     catggttttt caacatcaaa ccaaccatta ttaatattat cctttaaagg catccccaga      1320
     ctttcaatga cagaaccatc atcaccgaag tcttttgagt attcaccgcc tttaaactct      1380
     tcggttttct tatcaaacca atgaatatgt aatttaagcc ccatatttac ctcttaaaga      1440
     tattttttaa tgtttcgttt tggatccggc cctttaacct gcttacccgt ttttggatcg      1500
     aatgcgccga ggtgttcgcc atcactggcg cggtatcctt caagctcacc gtgctgggaa      1560
     tcccattcat aaattttacg ctttttatca ccataccatc gggcccgctt accacctcct      1620
     ccttgtttag gggttttagg ttttccctct ttcaactctc ccaacccctt aatatcttca      1680
     gtcttgggat caggaaaata atcatggcca taatctttag ttcccttgcg aggctttttc      1740
     ttttcctcat ttaatttatt ttctgcctcc ttcgcttttt tctccttttg tttccggctc      1800
     tccatcgccc cggaaagcgc agccttatct ctggataagg catcattcat tgcgtttaaa      1860
     gatgcctgag cagcttttaa tttcgcattc tgattctgaa tatccttatc aagatagcga      1920
     acctgatcct gccacccgga gccatattca tgaggagggt tataagtcac aaaatcatcc      1980
     ttattcgcct ccgcctcttt caccttttta tcagcttcct ggacagcagc cctggcgcca      2040
     ggaatcgctt tttctgtcga tgtaatcctg ccgtttaaag tcgtaatatt tttatcttcg      2100
     gcatccagct cagctttcgc tttatcatat tctcttttca gcccctcttc cggatgagcg      2160
     gcgtcccatg cctgctggcg acgcttttct tcctcctgac gctgttttac ctgtgccggg      2220
     gtaagaacat ctgtcaccga cacatagacc ggcttctgtc cggtctcttt cgggaaacga      2280
     ataacggcct cacgggagtt accaccggcc gtaaaaccag ccggacgtga atcacctttt      2340
     tcagcaacaa tgccttttgg cggggctttc gctgccggaa cacctttcgg tacgctcacc      2400
     tgcagagccg ggagacccgg aatagacaca ctgaataccc ccggacgttt tgtcggtttc      2460
     gcatccacca caggaacact cattggccga cccgcgacaa ccgcaatatg ctgccgttca      2520
     tccttcacca catccacaac ccgttgtctg acacgaaccg tcgcttggtc cagcggtaaa      2580
     gtacttgccg gagtctccgt caccgtatcg gccggtaatg acgtcataat ttttgacatc      2640
     attttcggat catcttttgc aatctcagaa ggcagtacac catagatcgc aatcccccac      2700
     agaccaaact taaacggacc tttcagggca gccagcacat cggcaacggc tgacgacagc      2760
     gcatcgccgg aaaaggataa agccggtcct tcagcccccg gtgtagccag ggcaggcaga      2820
     ccgaaggcca tcgcggtggc cgaagactgt ccaccaccag agctaccgct atgattgccg      2880
     ctgttaccac cattaccacc gggtttactc ccgccgttac caaactcccc cggattatag      2940
     ttatgaatat ctccattcgg cgtatgcgtc gtaccccagc ccggcccgct gtttggatcc      3000
     gtaccaccac ctgatgaagg gccaccttta ccagacgtac cgttcacatt cccactggcc      3060
     tgaccaccat tatgtcccag acctgaatta cccggacctc gaccgtctcc accactcata      3120
     tcgttttcct ctttgacatt taaaacaata aattaaaacc aaaatactgt atatatacac      3180
     agtatgatta ttaccttact cctccagttt tcaggtgtca atatatgaga ttagttctca      3240
     tcttcaggca caaaaaagcg cccggagccg cctgtaagca gatcgcgtct tttcccgaaa      3300
     atttctcatg gcaacaaatt ttattatttg agatattcat cacacttact tttatattgg      3360
     tatagatgta cctgaaagcg aaatggcatg aggagaggac ctgcggttaa actgttcggg      3420
     aaacctgaga cgcccggagg ccactatgac gcacctgaaa ctgactcccg ctgaagaaaa      3480
     gcgcctggcg gacgcctttg tcgctcaggc actggccatg ggcctgaaag ggaacaaaac      3540
     gctgccaaag gcggtaaaac aacaggtgcg ggaaagggtg cttcgtgcca ttcaggacga      3600
     acgtgaagcc aggcgggcgc aggaaatgcg tcgggagaaa tacgaggcgg cggaaaccac      3660
     cttcacctgg cagccgcagc gccgccggtg aaaacgattt aaggttctac tttcaggtgt      3720
     atccacttcg gaagcatttt agggatatgg tattatcgaa aatcatgctt tcggcaatcc      3780
     aatgggttta tacgatttaa aaagggaaaa acatggacaa aaggacaacg ttagtagctc      3840
     tggtttctat catctttttc acttctggat gtgatgacca gaaaaaaggg aatggcaacg      3900
     ctgaatcgac acaaatcact aaaaacgata taacaactca acttaagtta gataaaacgc      3960
     aacaacctga cgatatagaa aagaaaataa aagaaatcca ggccaactcc acccctgaaa      4020
     aaataaaaaa attcaatgaa ttaaaagatg acgaccctaa aatcctacga gagtcggttg      4080
     tttcagaaat taagaaaaaa ctaccactat tggttgacga agccacatta atgacagatg      4140
     tatcaactga cggaggtaca ttctcataca aatatgtaac caaagggatt tcagccagca      4200
     cgatggaaag tgatgtgtgg aaagatgcca tgcaaaaaaa cataaaaaat agctactgct      4260
     ctgacgatgc cagactaaaa gtattcagag agctatttcc tgaaggtgtc atttacaact      4320
     actacttaag tgacaaatta atctacacat acaaggcact tccatcgatt tgttcctaaa      4380
     gtatttacca taacgtcgca agctgggcgc cccagcgggc ggcttttcta tatctgtcct      4440
     ataaattcag aaaaggaaag ctaaatggct gcatatacca gcgaaacagc gaagttttta      4500
     caaagcgtca gtagcgatca aacatggtgg aagtcacgat ataatccagg agacagcgtt      4560
     ccccattctg gcatttatcg atgtacagtc tgcggaaaag agatcaccag taacggcaac      4620
     gacccgttcc cgccgcaaaa ccaccatcag cacagtcagc aacaagcaat caaatggcaa      4680
     ctgattgtca gaacggacac taaaggtgac cgtttcggaa tcaaatgatt gagagccatc      4740
     cggagctttt tttgtacctg aaactcgaag atatttacca taacatccct tatcggtacc      4800
     gccagtaagg cactggcggc gtttccggcg gctacgggat aaaacaagca acaacccacc      4860
     cggaaaaccg ccggaaacgc gcccggtaaa ttttatccta atgcatgcct atgcacgcaa      4920
     ctgcatagca tatgttttga cgtaaaatgg atgccgtaag cccttttttt accgactttc      4980
     tgcttcatca gcctgactga cacgaccata ttctttcaga ccgccccctg ctaaacttct      5040
     tcatatcacc gcattaaccc gggagctccg caatgagcaa cactacacac tacgaaaacg      5100
     ccaacttctg cgggaactgg ctgaaaacct gccccgcgta gaccgccgga aggctggccc      5160
     gcaccaaagc cgccgcaacg cctggctaac gaagaactgg cccaggcaga gtatgaagat      5220
     caggttcgcg ctaaagtgac cgccgcccgg cctgacacgc gctctggcat gaccacggaa      5280
     caacttcgcc agagactgca tggacgctat caggaactac gcgatgccgt atgaggtgga      5340
     atggaagcag ggagccatcg aggatgtgac agcattgttt gactacattg cggaaaattc      5400
     atcctgtggg atgcgcagaa accgttaccg atcgcgttct cgctgcggcg gacaaactgg      5460
     ccgacttccc gcggccagcg gccagaatcc ccataccgtt agctaataac cgatgacggg      5520
     ggcgcaattc atttgcgtgg ccgtcgccgg ttggacaagc gcagcgcgtc agcgttttcc      5580
     ccagtgccac caccggcgcg acgtttcggg atgtgtatct gctgtctccg gtttgtgttc      5640
     cagcagtctc atggcctgca tttcctgcgc agtccggcta cctcttcccg cagcgcttta      5700
     atctccgaaa tcagtgcctc agacaccgat gcttcagtct cacccggcag tctcaacccg      5760
     tcccgctcct gagtctcacc ctgtcttaat tcaccgtact cccggataag ctcgctgtat      5820
     ccaccacccg accaccatca gcttccgtac ggtatgatac gcggcctttc gccatatcac      5880
     gatacagaga actccgggct ttccccgtca gttccgtcgc ctgtctcacc gtcacccacg      5940
     ccattgtctc acctccggtt ttgagacagt atacccgaca gaagcacacc acacccggaa      6000
     gacagaaaac atgacgaagc aggaaaaaac tgccctcaat atggctaaat tcattaagga      6060
     ccagtcgctg ctgttgcctc gagaaactga acgagctgga tatggatacc ccggccgaca      6120
     tgtgcgagcg gcttcacgag gatgcggaaa cactctggca ggtgatgcgg gataagctcg      6180
     ggaaggaata acagcaccgg ccatgccggt gcgccgttta tttttgcgac gggccaccac      6240
     cggcgggcgg gtttgtcctc cggcagttca ggcaccggca cgttaaaata gcggctgtta      6300
     tattcccgca tcagctccgc tttcagcttc tggttcaccg cgctttcccc gtgccgctcg      6360
     cttaccacct caatcatcgc gttaatctgc tccggtgagc acttcccggc atcaaccacc      6420
     tgcacaccgt tctcgatggc gaaccgctcc accttgcgat agaggtaatc actcagcttc      6480
     agttcctgtg ggtttgtccc gttcccattg cggattttca ccggtgaaaa agtacgtggc      6540
     tttttcgtcg ctgttgagcc gctcgtcata catcccgatg gatttcatca tcgccagatg      6600
     ccgggcccgg tcgacaaaac agaaaacata cgccccggtc tgccaggcga gcaccaccat      6660
     atcttcaaga tgctcgctgg tcggcatgat gcagcgctcg ccgtcccggt cctccgcgtc      6720
     ttcaataaat accatgtaca gcgccataat ccctcactcc gttgtcataa atttaaagca      6780
     cgcaaacagc cgttcacgat ccagctccgc cgttttgcgg gcctcgccgg tctgtggact      6840
     cacgatttca tccaccgaca ggatcgcccg ctgcaccctg acctgcgtcg cccggtccgg      6900
     gcagatgtac cagatttcat cgatgccgtt cgcccggcgg ttaaacagat acccggacac      6960
     cacctcctga tagcgctgcg gctcttcacg gtgcgctcca cctcaaaagc caccgttctg      7020
     ccgtccagct ccgccagccg gtccggcacc ttcatccctt tcagggcaat acggtgcagg      7080
     taccgccggc cggtgccccc cgggaattgc cctccatggc gaggcgcagg agctcgaacg      7140
     gccagccggt tcgcggcagc tgcgcgcaga caccgcgacg gcatgaaacg gaataatgtc      7200
     cgtcagcggc tcgtcctcgt cgaacgagaa cgccaccccg tgcggcgtca gccccacacg      7260
     ctgacctgac ggccgtcaac cgggttattt cgcccgacca gcagcccttt cgccaccagg      7320
     tgtccaggaa gcggtaaacc ggctgtaccg ccgcaatccc cagccgtcgc gcaaccagct      7380
     ccgccgtgga acaagctctt cccgcagcca gcgcaggatc agccgtgttt tctccgcgtt      7440
     ccgcctgccg cttcggctgg ctgacgttgt atcgctccag tgccatcgta cagccccgca      7500
     aaatcatcat caccgcccgt gccgcctctc cggctggtac tccgccagcg ggtccgtttt      7560
     cgccagcacc gggaaggttt tcagcggaat ttcccggcga tcaacccgca gcggctctga      7620
     acgcagccgc gccacgccag cccggtcatc accgcgaacc cgtcagcaga tgttgcaggg      7680
     tgttcacgtc aaacagcggc cggctccttc tggatgaagc tgtccccgct gatatgctcc      7740
     acgttccccg cctcccggct ggcccgcggc gctccacgtc cacccggatt tccccggtct      7800
     ggcccgcgcc actgcgcgac tcctggctgg ccgcccggta gaaccagcgg atcggcgtgt      7860
     tatccagcac cgtggttttc acaaaatcag cgggcagatc ctggccagct gcccgaagtc      7920
     gcccagcgtg gtgcgcgaga cgcaggttgc agtcccggct gcgcacggtc cccagcgcgt      7980
     tcagcacgta cttcgacagc aggtatttaa tctcatcgag catgatgctc gcgtgcggcc      8040
     acctccggaa ctcatcccgc gcaatgatga tttgtgcgca gcgggcaaac agcatcttct      8100
     gcacccggat caccgcctca tcgtccatcg agccgatgac gtacagcagc cggcgccgtt      8160
     gattatcccc gccacgtcga tgccgctgtc cgtctgcagg acgctcaggt cggccacgtt      8220
     ctccagcttg gtgatcagcc ccttcacgtc ctttttcagg gcctccggca gcgcgtacgc      8280
     cgccgccagc acctgctgaa tgtttgcccc ctgcggaaac ctgctccgca atcagcttcg      8340
     ccgctttctg ctcgctgatg cggtagtggt ccgccacgtg cccggtctca ctcaggttaa      8400
     acccggcaac cagcagattc ttcagggcgt actggtcgag atcgcggaac agattcagct      8460
     gcgccggttt ccccggccgc agatccagca gggtgaagtt tgggcagtgc gccggaagga      8520
     gcgcggccac cggtcccctt tggtcgaagt acaccaccgc gtccccgaaa tcgcgtaccg      8580
     agcgaatgag cgcgttggtg gccatcacgc tcttgccgct cccgggcagg ccgaggatct      8640
     gaatgttggt cttgcgccag gtatcacgcg ggaccagcac cggctcgccc tgctcatcct      8700
     tacccagaaa cacgcctgct gtttccgggc ctcgtccgtg gaaaaatcgg gccagcgcgt      8760
     tgacggtacc ggctccccga aatgcaggtt ggcggacgtc ggtcatttca ccggtcgctg      8820
     gctgtgcgcc ggctctcctg ttcagtttcc ggttcaccgc ctcgtcagca gcggcaaaat      8880
     gaccgtcaag cagcagaacg ccagagccgc gccaacacaa tcccgacaac cagcccgccc      8940
     gcgcaccagg caatcccgaa caggttagcc ggctgcagca ggtaataatc tgcgttcgcg      9000
     tagaggtccg gacgcaggac ggtttctccc cagctctcga taaacacggc gacaaccagc      9060
     ccccccagaa ggccagacgg gctgtgacgc cagtacgcgt agttccagac atgccgccgt      9120
     gccctgacca tccgcccggg cgaatccagc gaggccagca cgaaaatggc gcagtccggt      9180
     tattatcgcg cttatacgat cggccatact cccgtcgctc cttatgactc agccccacaa      9240
     aacgcatccg gcccagagtt cagccagacc gaaaaacagg ccgataacca ggaaagcccc      9300
     ggccagaccg taatactccc tgtaaagcca gctgacccac gggtaaatct gccggggcca      9360
     gctccgggat atgccactgc caggtaagcg aatccgtatt aaacatgtaa tctctctcct      9420
     tcagacgacg ggtttaccgg cgtggtgtgc agcccccgct gcacaagccg cggacatgcc      9480
     ccaagggatg gcggcggcgt tcacgtccgt ggtgactgcg ttcagctttc accacggagg      9540
     ccagcctctt tccgccccat gcggaaaagg cgcagcccgg aggaacaacg agcacgcccc      9600
     agcgtgtgag aggaaaaggc ggcaccccag tgcggtcttt tccggtgttc cggcccccgt      9660
     cagagttcgc catagcgagc ggctgaccac ccgcaaaatc agaggcagct ttgctgcgtc      9720
     gtcaggtgcg tccagcaccg tggatttttg tgggtggtcg ggcgtgagcc tgacgggggc      9780
     cgggtgaatt tctggcgtgg ccggcgtcca gccgacagaa ccagaaagag ggggaaagac      9840
     cgtgcgtcgg tggcgtccag ccagcgtaag caggggattg ccccctgaag tcagccacgc      9900
     cccagcgggg atgacttgca tttctccgca aggagaaaaa agcaacagag ataggtt         9957
//