ID   W41549; SV 1; linear; mRNA; EST; MUS; 1115 BP.
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AC   W41549;
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DT   21-MAY-1996 (Rel. 47, Created)
DT   04-MAR-2000 (Rel. 63, Last updated, Version 2)
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DE   mc50c10.r1 Soares mouse p3NMF19.5 Mus musculus cDNA clone IMAGE:351954 5'
DE   similar to gb:L07037 Mus musculus (MOUSE);, mRNA sequence.
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KW   EST.
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OS   Mus musculus (house mouse)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
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RN   [1]
RP   1-1115
RA   Marra M., Hillier L., Allen M., Bowles M., Dietrich N., Dubuque T.,
RA   Geisel S., Kucaba T., Lacy M., Le M., Martin J., Morris M.,
RA   Schellenberg K., Steptoe M., Tan F., Underwood K., Moore B., Theising B.,
RA   Wylie T., Lennon G., Soares B., Wilson R., Waterston R.;
RT   "The WashU-HHMI Mouse EST Project";
RL   Unpublished.
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DR   MD5; 37cb23c37c02c5674815adfba9201c72.
DR   UNILIB; 637; 431.
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CC   On Apr 14, 1993 this sequence version replaced gi:692633.
CC   Contact: Marra M/Mouse EST Project
CC   WashU-HHMI Mouse EST Project
CC   Washington University School of MedicineP
CC   4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
CC   Tel: 314 286 1800
CC   Fax: 314 286 1810
CC   Email: mouseest@watson.wustl.edu
CC   This clone is available royalty-free through LLNL ; contact the
CC   IMAGE Consortium (info@image.llnl.gov) for further information.
CC   MGI:223754
CC   Seq primer: ETPrimer
CC   High quality sequence stop: 436.
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FH   Key             Location/Qualifiers
FH
FT   source          1..1115
FT                   /organism="Mus musculus"
FT                   /lab_host="DH10B (ampicillin resistant)"
FT                   /mol_type="mRNA"
FT                   /dev_stage="19.5 dpc total fetus"
FT                   /clone_lib="Soares mouse p3NMF19.5"
FT                   /clone="IMAGE:351954"
FT                   /note="Vector: pT7T3D (Pharmacia) with a modified
FT                   polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA
FT                   was primed with a Not I - oligo(dT) primer [5'
FT                   TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'],
FT                   double-stranded cDNA was size selected, ligated to Eco RI
FT                   adapters (Pharmacia), digested with Not I and cloned into
FT                   the Not I and Eco RI sites of a modified pT7T3 vector
FT                   (Pharmacia). Library went through one round of
FT                   normalization to a Cot = 5. Library constructed by Bento
FT                   Soares and M.Fatima Bonaldo. RNA was kindly provided by Dr.
FT                   Minoru Ko (Wayne State University)."
FT                   /db_xref="taxon:10090"
FT                   /db_xref="UNILIB:637"
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SQ   Sequence 1115 BP; 224 A; 258 C; 332 G; 299 T; 2 other;
     gaaagtaagg accaatgatc ataaggtgct gggggaggtg cgggtacagt acactagcag        60
     agacagcttc aaggcctttg ctaaggccct gggtgtcatg gatgacctca agtctggtgt       120
     ccccagggct ggctaccgtg gcattgtcac tttccagttt cggggccggc gtgtgcacct       180
     ggcaccccca cagacatgga ctggctatga tcctagctgg aattagcagc acctgccttt       240
     ccctcctggg tctccttgcc gcgtgctgag ccgaggcagg cctgcagggc tgggctacac       300
     catcctgcca gtgtctccct ctcagtgtct cctcttggat ctgtagatct tccctttgtg       360
     tcctggccag cccccaaatg gcattctagt gcacaaatca taggatgaga gttatactcc       420
     tgttgtcaag ggagtattgt gtggtatgtt cggggcatat tgaacaagaa accactgtgt       480
     ggtatgggga gggttgggcc tgttggggca aggtttgtaa tgtctcgaag ggcatctgca       540
     gagagcttgg cagctccagt ctcctctaga agccttctca acgtggatct gggccctgtt       600
     aacccatgag ccctctttaa tgtccgatcg tccctttctg aaacttccct cagggttgng       660
     gctggaatta tggaggaagg aatggacttt tgctagaatg ccttcatggg gagccctgct       720
     agagttactg tgatatgttc tcccggggaa tcctcggggg ttctcccctg ggagtgtggc       780
     ttgtctgtgg aagtattctg tttttgagaa aggagaaaag aactctgtgg ggcgaggcta       840
     aaggtgttat tgtgcagggg ggcgctccct cttttagtag gacggcctct tccaccatgt       900
     gattgcgcca tgaggtgaca atcttaggct acgcccgtga aagaaaaaat gtcggagggc       960
     gctcacacgt ccaagcagag ggtggaaaac tttgtctgtc tagtacaatt ttatcggtag      1020
     aaaaaaaaaa agtgttgtgg tcgtgggact cctttgggcc gatttgaggc ccccccctgt      1080
     gtgataattg cgngttcttg gttgtggtgg ttgtt                                 1115
//