ID   W33544; SV 1; linear; mRNA; EST; MUS; 352 BP.
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AC   W33544;
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DT   16-MAY-1996 (Rel. 47, Created)
DT   04-MAR-2000 (Rel. 63, Last updated, Version 3)
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DE   mc52e08.r1 Soares mouse embryo NbME13.5 14.5 Mus musculus cDNA clone
DE   IMAGE:352166 5' similar to gb:D13748 EUKARYOTIC INITIATION FACTOR 4A-I
DE   (HUMAN); gb:X03039_cds1 Mouse mRNA for initiation factor eIF-4AI (MOUSE);,
DE   mRNA sequence.
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KW   EST.
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OS   Mus musculus (house mouse)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
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RN   [1]
RP   1-352
RA   Marra M., Hillier L., Allen M., Bowles M., Dietrich N., Dubuque T.,
RA   Geisel S., Kucaba T., Lacy M., Le M., Martin J., Morris M.,
RA   Schellenberg K., Steptoe M., Tan F., Underwood K., Moore B., Theising B.,
RA   Wylie T., Lennon G., Soares B., Wilson R., Waterston R.;
RT   "The WashU-HHMI Mouse EST Project";
RL   Unpublished.
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DR   MD5; 8a0dfc4e4ef226584084c49d9ca83363.
DR   UNILIB; 562; 437.
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CC   On Sep 21, 1992 this sequence version replaced gi:276140.
CC   Contact: Marra M/Mouse EST Project
CC   WashU-HHMI Mouse EST Project
CC   Washington University School of MedicineP
CC   4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
CC   Tel: 314 286 1800
CC   Fax: 314 286 1810
CC   Email: mouseest@watson.wustl.edu
CC   This clone is available royalty-free through LLNL ; contact the
CC   IMAGE Consortium (info@image.llnl.gov) for further information.
CC   MGI:223966
CC   Trace considered overall poor quality
CC   Seq primer: -28M13 rev2 from Amersham
CC   High quality sequence stop: 1.
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FH   Key             Location/Qualifiers
FH
FT   source          1..352
FT                   /organism="Mus musculus"
FT                   /lab_host="DH10B"
FT                   /strain="C57BL/6J"
FT                   /mol_type="mRNA"
FT                   /sex="unknown"
FT                   /dev_stage="13.5-14.5dpc total fetus"
FT                   /clone_lib="Soares mouse embryo NbME13.5 14.5"
FT                   /clone="IMAGE:352166"
FT                   /tissue_type="embryo"
FT                   /note="Vector: pT7T3D-Pac (Pharmacia) with a modified
FT                   polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA
FT                   was primed with a Not I - oligo(dT) primer [5'
FT                   TGTTACCAATCTGAAGTGGGAGCGGCCGCGGAAATTTTTTTTTTTTTTTTTTTTTTTT
FT                   T 3'], on equal amounts of mRNA from 2 13.5dpc and 2
FT                   14.5dpc embryos [total RNA provided by  Minoru Ko, Wayne
FT                   State Univ., from 2 ]; double-stranded cDNA was ligated to
FT                   Eco RI adaptors (Pharmacia), digested with Not I and cloned
FT                   into the Not I and Eco RI sites of the modified pT7T3
FT                   vector. Library went through one round of normalization,
FT                   and was constructed by Bento Soares and M.Fatima Bonaldo. 
FT                   "
FT                   /db_xref="taxon:10090"
FT                   /db_xref="UNILIB:562"
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SQ   Sequence 352 BP; 96 A; 79 C; 97 G; 80 T; 0 other;
     caacaccaga aggaaggtgg actggctcac cgagaagatg catgcccgag atatcactgt        60
     gtctgcgatg cacggagata tggaccataa ggaacgagat gtgatcatga gggagttccg       120
     gtctggctct agcagagtat taattaccac tgacctgttg gccagagcat tagatgtgca       180
     gcaggtctcc ttagtcatca actatgacct tcccaccaac agggagaact acatccacag       240
     aatcggtcga ggtggtcggt ttggtcgtaa gggtgtggct attaacatgg tgacgaagga       300
     agacagaagg actcttcgag acctttgagc tttcctagaa caccttccat tg               352
//