ID   W30895; SV 1; linear; mRNA; EST; HUM; 411 BP.
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AC   W30895;
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DT   13-MAY-1996 (Rel. 47, Created)
DT   31-JAN-2011 (Rel. 107, Last updated, Version 6)
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DE   zb78e12.r1 Soares_senescent_fibroblasts_NbHSF Homo sapiens cDNA clone
DE   IMAGE:309742 5', mRNA sequence.
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KW   EST.
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OS   Homo sapiens (human)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
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RN   [1]
RP   1-411
RX   PUBMED; 8889549.
RA   Hillier L., Lennon G., Becker M., Bonaldo M.F., Chiapelli B., Chissoe S.,
RA   Dietrich N., DuBuque T., Favello A., Gish W., Hawkins M., Hultman M.,
RA   Kucaba T., Lacy M., Le M., Le N., Mardis E., Moore B., Morris M.,
RA   Parsons J., Prange C., Rifkin L., Rohlfing T., Schellenberg K.,
RA   Soares M.B., Tan F., Thierry-Meg J., Trevaskis E., Underwood K.,
RA   Wohldmann P., Waterston R., Wilson R., Marra M.;
RT   "Generation and analysis of 280,000 human expressed sequence tags";
RL   Genome Res. 6(9):807-828(1996).
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DR   MD5; 119d9182b13ff1142c5bfd4e0b262961.
DR   UNILIB; 117; 423.
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CC   Contact: Wilson RK
CC   Washington University School of Medicine
CC   4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
CC   Tel: 314 286 1800
CC   Fax: 314 286 1810
CC   Email: est@watson.wustl.edu
CC   This clone is available royalty-free through LLNL ; contact the
CC   IMAGE Consortium (info@image.llnl.gov) for further information.
CC   Insert Length: 706   Std Error: 0.00
CC   Seq primer: mob.REGA+ET
CC   High quality sequence stop: 270.
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FH   Key             Location/Qualifiers
FH
FT   source          1..411
FT                   /organism="Homo sapiens"
FT                   /lab_host="DH10B (ampicillin resistant)"
FT                   /mol_type="mRNA"
FT                   /clone_lib="LIBEST_000423
FT                   Soares_senescent_fibroblasts_NbHSF"
FT                   /clone="IMAGE:309742"
FT                   /tissue_type="senescent fibroblast"
FT                   /note="Vector: pT7T3D (Pharmacia) with a modified
FT                   polylinker V_TYPE: phagemid; Site_1: Not I; Site_2: Eco RI;
FT                   1st strand cDNA was primed with a Not I - oligo(dT) primer
FT                   [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'],
FT                   double-stranded cDNA was size selected, ligated to Eco RI
FT                   adapters (Pharmacia), digested with Not I and cloned into
FT                   the Not I and Eco RI sites of a modified pT7T3 vector
FT                   (Pharmacia). Library went through one round of
FT                   normalization to a Cot = 5. Library constructed by Bento
FT                   Soares and M.Fatima Bonaldo."
FT                   /db_xref="taxon:9606"
FT                   /db_xref="UNILIB:117"
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SQ   Sequence 411 BP; 140 A; 69 C; 77 G; 122 T; 3 other;
     gcacaacaag tgattgtcca gggtgtccat gagctctatg atctggagga gactccagtc        60
     gagctggaag gatgacactg agagaacaaa tcgattggtc ctccttggca gaaatttaga       120
     taaggatatc cttaaacagc tgtttatagc tactgtgaca gaaacagaaa agcagtggac       180
     aacacatttc aaagaagatc aagtttgtac ataacactag aggcatttct tatcaaaagg       240
     attggataat aanaataagt ttctactggg tatatttcaa gcatttattt attactttag       300
     ttaccgaatt ccaatatact ttaaaantgg tatttgtttt acagcatacc taaaatggta       360
     gcaaatccgg tactgtaaaa cctttnacct tcctacagtt atatatgata t                411
//