ID   W28128; SV 1; linear; mRNA; EST; HUM; 615 BP.
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AC   W28128;
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DT   14-MAY-1996 (Rel. 47, Created)
DT   04-MAR-2000 (Rel. 63, Last updated, Version 2)
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DE   42f4 Human retina cDNA randomly primed sublibrary Homo sapiens cDNA, mRNA
DE   sequence.
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KW   EST.
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OS   Homo sapiens (human)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
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RN   [1]
RP   1-615
RA   Macke J., Smallwood P., Nathans J.;
RT   "Adult Human Retina cDNA";
RL   Unpublished.
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DR   MD5; d8ca838f3f96474dcdd6cc06cfc7446b.
DR   UNILIB; 543; 433.
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CC   On May 5, 1995 this sequence version replaced gi:798193.
CC   Contact: Dr. Jeremy Nathans
CC   Dr. Jeremy Nathans, Dept. of Molecular Biology and Genetics
CC   Johns Hopkins School of Medicine
CC   725 North Wolfe Street, Baltimore, MD 21205
CC   Tel: 410 955 4678
CC   Fax: 410 614 0827
CC   Email: jeremy_nathans@qmail.bs.jhu.edu
CC   Clones from this library are NOT available.
CC   PCR PRimers
CC   FORWARD: CTTTTGAGCAAGTTCAGCCTGGTTAAGT
CC   BACKWARD: GAGGTGGCTTATGAGTATTTCTTCCAGGGTAA
CC   Seq primer: GGGTAAAAAGCAAAAGAATT.
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FH   Key             Location/Qualifiers
FH
FT   source          1..615
FT                   /organism="Homo sapiens"
FT                   /lab_host="E. coli strain K802"
FT                   /mol_type="mRNA"
FT                   /sex="mixed (males and females)"
FT                   /dev_stage="adult"
FT                   /clone_lib="Human retina cDNA randomly primed sublibrary"
FT                   /tissue_type="retina"
FT                   /note="Organ: eye; Vector: lambda gt10; Site_1: EcoRI;
FT                   Site_2: EcoRI; The library used for sequencing was a
FT                   sublibrary derived from a human retina cDNA library.
FT                   Inserts from retina cDNA library DNA were isolated,
FT                   randomly primed, PCR amplified, size-selected, and cloned
FT                   into lambda gt10.  Individual plaques were arrayed and used
FT                   as templates for PCR amplification, and these PCR products
FT                   were used for sequencing."
FT                   /db_xref="taxon:9606"
FT                   /db_xref="UNILIB:543"
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SQ   Sequence 615 BP; 152 A; 104 C; 108 G; 178 T; 73 other;
     tnnnnncgcn catgacactg actttnngag aagcccaagt caatcatctt atagaatgac        60
     tacattcagg atttgtctaa taatttcccc atgattaaat tccagtttaa cacttggtgc       120
     aagaaaacta aataggtaaa attcttaagt cacatcagga ggggcataat gttagggtgt       180
     nccaccttgg gtgatgctaa attgaatcac ttggttattg tggtgaccat tgtaaaggta       240
     cattttcccc tgtataattg atacctcttc tgtaggatta tatttaaatc ctngtngaag       300
     atcctattcc acaatagctt ttcatccaat agttttagca tctattgatg aacgttacgt       360
     gaaaaatcta tgaactgctc ttttatgaac anntttttaa agnctttata aaactatttc       420
     ctcttactgg ttttncaact tngggggtaa nccnaatttg ggcccnttta aaatcccnaa       480
     atcaattgaa ggttngaatg gngntngngg tgttttnnng gaattgggnc cccanccaan       540
     tggattggnc ccnggggggg gncccnggtt cngcccnnnn nnnnnnnnnn nnnnnnnnnn       600
     nnnncnnnnn nnnnc                                                        615
//