ID   W28125; SV 1; linear; mRNA; EST; HUM; 602 BP.
XX
AC   W28125;
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DT   14-MAY-1996 (Rel. 47, Created)
DT   04-MAR-2000 (Rel. 63, Last updated, Version 2)
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DE   42d11 Human retina cDNA randomly primed sublibrary Homo sapiens cDNA, mRNA
DE   sequence.
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KW   EST.
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OS   Homo sapiens (human)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
XX
RN   [1]
RP   1-602
RA   Macke J., Smallwood P., Nathans J.;
RT   "Adult Human Retina cDNA";
RL   Unpublished.
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DR   MD5; f54fc0ccd1ea25dcd9770de270066b48.
DR   UNILIB; 543; 433.
XX
CC   On May 5, 1995 this sequence version replaced gi:798172.
CC   Contact: Dr. Jeremy Nathans
CC   Dr. Jeremy Nathans, Dept. of Molecular Biology and Genetics
CC   Johns Hopkins School of Medicine
CC   725 North Wolfe Street, Baltimore, MD 21205
CC   Tel: 410 955 4678
CC   Fax: 410 614 0827
CC   Email: jeremy_nathans@qmail.bs.jhu.edu
CC   Clones from this library are NOT available.
CC   PCR PRimers
CC   FORWARD: CTTTTGAGCAAGTTCAGCCTGGTTAAGT
CC   BACKWARD: GAGGTGGCTTATGAGTATTTCTTCCAGGGTAA
CC   Seq primer: GGGTAAAAAGCAAAAGAATT.
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FH   Key             Location/Qualifiers
FH
FT   source          1..602
FT                   /organism="Homo sapiens"
FT                   /lab_host="E. coli strain K802"
FT                   /mol_type="mRNA"
FT                   /sex="mixed (males and females)"
FT                   /dev_stage="adult"
FT                   /clone_lib="Human retina cDNA randomly primed sublibrary"
FT                   /tissue_type="retina"
FT                   /note="Organ: eye; Vector: lambda gt10; Site_1: EcoRI;
FT                   Site_2: EcoRI; The library used for sequencing was a
FT                   sublibrary derived from a human retina cDNA library.
FT                   Inserts from retina cDNA library DNA were isolated,
FT                   randomly primed, PCR amplified, size-selected, and cloned
FT                   into lambda gt10.  Individual plaques were arrayed and used
FT                   as templates for PCR amplification, and these PCR products
FT                   were used for sequencing."
FT                   /db_xref="taxon:9606"
FT                   /db_xref="UNILIB:543"
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SQ   Sequence 602 BP; 136 A; 139 C; 121 G; 63 T; 143 other;
     tcnnnngccc caatgnaaat acaccancga tggagtatgc gtacgtcttc cagtaaaaaa        60
     ggacaatata aataaacatc tncaagaacc tctcagggac gtctgcacgt ctactgtggg       120
     gcgcacagca cagatggacg caggggctgg ggctccaggg cagttgctgc cacacccgcc       180
     ggggtcctac cacactacac tcagaaacgt cacacggagt gggaaaaaag ccacagcttg       240
     ctcagctncc gacaaagagc ggattcacag accgcccgat ctcagagaag tgagnggggc       300
     acccncccca aggnntnntt tttttttttt tttttgggga nngnanccan nnncnaaaan       360
     aaaacccaaa accccccana accccngnnn nnngggggna ancccaaggc ccgggggnna       420
     aaaaggnaaa ngcnnnccaa anggnggggg gaacccccaa aaaaanaaag aacccccnan       480
     gggggggncc cnngcncggg ccccnnnnnn nnnnnnnnnn nnnnnncccc nncnnnnnnn       540
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       600
     nn                                                                      602
//