ID   W24643; SV 1; linear; mRNA; EST; HUM; 238 BP.
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AC   W24643;
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DT   09-MAY-1996 (Rel. 47, Created)
DT   31-JAN-2011 (Rel. 107, Last updated, Version 6)
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DE   zb62h05.r1 Soares_fetal_lung_NbHL19W Homo sapiens cDNA clone IMAGE:308217
DE   5' similar to gb:J00314_rna2 TUBULIN BETA-1 CHAIN (HUMAN), mRNA sequence.
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KW   EST.
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OS   Homo sapiens (human)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
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RN   [1]
RP   1-238
RX   PUBMED; 8889549.
RA   Hillier L., Lennon G., Becker M., Bonaldo M.F., Chiapelli B., Chissoe S.,
RA   Dietrich N., DuBuque T., Favello A., Gish W., Hawkins M., Hultman M.,
RA   Kucaba T., Lacy M., Le M., Le N., Mardis E., Moore B., Morris M.,
RA   Parsons J., Prange C., Rifkin L., Rohlfing T., Schellenberg K.,
RA   Soares M.B., Tan F., Thierry-Meg J., Trevaskis E., Underwood K.,
RA   Wohldmann P., Waterston R., Wilson R., Marra M.;
RT   "Generation and analysis of 280,000 human expressed sequence tags";
RL   Genome Res. 6(9):807-828(1996).
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DR   MD5; 66ae415e02c7ba24e4ab6cbd03517d41.
DR   UNILIB; 121; 415.
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CC   Contact: Wilson RK
CC   Washington University School of Medicine
CC   4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
CC   Tel: 314 286 1800
CC   Fax: 314 286 1810
CC   Email: est@watson.wustl.edu
CC   This clone is available royalty-free through LLNL ; contact the
CC   IMAGE Consortium (info@image.llnl.gov) for further information.
CC   Putative full length read
CC   Insert Length: 330   Std Error: 0.00
CC   Seq primer: mob.REGA+ET
CC   High quality sequence stop: 176.
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FH   Key             Location/Qualifiers
FH
FT   source          1..238
FT                   /organism="Homo sapiens"
FT                   /lab_host="DH10B (ampicillin resistant)"
FT                   /mol_type="mRNA"
FT                   /dev_stage="19 weeks"
FT                   /clone_lib="LIBEST_000415 Soares_fetal_lung_NbHL19W"
FT                   /clone="IMAGE:308217"
FT                   /note="Organ: lung; Vector: pT7T3D (Pharmacia) with a
FT                   modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st
FT                   strand cDNA was primed with a Not I - oligo(dT) primer
FT                   [5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCAATTTTTTTTTTTTTTTTTT-3'],
FT                   double-stranded cDNA was size selected, ligated to Eco RI
FT                   adapters (Pharmacia), digested with Not I and cloned into
FT                   the Not I and Eco RI sites of a modified pT7T3 vector
FT                   (Pharmacia). Library went through one round of
FT                   normalization to a Cot = 5. Library constructed by Bento
FT                   Soares and M.Fatima Bonaldo. This library was constructed
FT                   from the same fetus as the fetal heart library, Soares
FT                   fetal heart NbHH19W."
FT                   /db_xref="taxon:9606"
FT                   /db_xref="UNILIB:121"
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SQ   Sequence 238 BP; 45 A; 55 C; 62 G; 70 T; 6 other;
     tcagncagca naagaggagg aggatttcgg tgaggaggcc gaagaggagg cctaaggaga        60
     gccccatcac ctcaggcttc tcagttccct tagccgtctt actcaactgc ccctttcctc       120
     tccctcagaa tttgtgtttg ctgcctctat cttgtttttt gttttttctt ctgggggggg       180
     tctagaacag tgcctggcac atatgtaggg gcgccnatna atnantggtt tgtttgaa         238
//